Method for treatment of tissue

l

R' 5 - Semialdehyde

C=0 i

R' Lysine a - Amine Adipic Acid • R

R

NH i

CH o - CH

i

NH

____Aldol_____^H ... C=CH-CH2-CH2-CH2 £ ... C^e^<L0 CHO ^

R Cross Link

FIG. 3

R
i

NH
l

CH _ CH 2 - CHO + NH 2 - CH 2 - CH - CH 2 - CH 2 - CH

OH 9=°

R'

Derived Aldehyde Hydroxylysine

R
I

NH

CH CH 2 - CH=N - CH 2 - CH - CH 2 - CH 2 - CH

C=0

I

Reduction

R

2

OH

p Lobile Schiff Base ,

? NH

NH I

CH -CH2-CH2-CH2-CH2-NH-CH2-CH -CH2-CH2- CH

1 OH C=0

CO OH i

i Stable Crosslink R'

R'

FIG. 4