US 20030194740A1
(19) United States
(12) Patent Application Publication (io) Pub. No.: US 2003/0194740 Al
Williams (43) Pub. Date: Oct. 16,2003
(54) SYSTEM AND METHOD FOR NUCLEIC ACID SEQUENCING BY POLYMERASE SYNTHESIS
(75) Inventor: John G.K. Williams, Lincoln, NE (US)
Correspondence Address:
TOWNSEND AND TOWNSEND AND CREW,
LLP
TWO EMBARCADERO CENTER
EIGHTH FLOOR
SAN FRANCISCO, CA 94111-3834 (US)
(73) Assignee: LI-COR, Inc., Lincoln, NE
(21) Appl. No.: 10/438,639
(22) Filed: May 14, 2003
Related U.S. Application Data
(62) Division of application No. 09/859,104, filed on May 14, 2001, which is a division of application No. 09/460,303, filed on Dec. 13, 1999, now Pat. No. 6,255,083.
(60) Provisional application No. 60/112,078, filed on Dec. 14, 1998. Provisional application No. 60/115,496, filed on Jan. 11, 1999.
Publication Classification
(51) Int. CI.7 B05D 3/00; C12Q 1/68;
C12P 19/34
(52) U.S. CI 435/6; 435/91.2; 427/2.11
(57) ABSTRACT
This invention relates to improved methods for sequencing and genotyping nucleic acid in a single molecule configuration. The method involves single molecule detection of fluorescent labeled PPi moieties released from NTPs as a polymerase extension product is created.
