United States Patent m
Walter
[ii] 4,273,868 [45] Jun. 16, 1981
[54] COLOR STABLE GLUCOSE TEST
[75] Inventor: Bert Walter, South Bend, Ind.
[73] Assignee: Miles Laboratories, Inc., Elkhart, Ind.
[21] Appl. No.: 14,500
[22] Filed: Feb. 23, 1979
[51] Int. C1.3 C12Q 1/54
[52] U.S. CI 435/14; 435/28
[58] Field of Search 435/14, 28, 25
[56] References Cited
U.S. PATENT DOCUMENTS
2,905,594 9/1959 Morris 435/28
3,350,278 10/1967 Gretton et al 435/14
3,853,470 12/1974 Mora et al 435/14 X
FOREIGN PATENT DOCUMENTS
1464359 2/1977 United Kingdom .
1464360 2/1977 United Kingdom .
Primary Examiner—Thomas G. Wyse
Attorney, Agent, or Firm—Charles J. Herron
[57] ABSTRACT
Test means, such as a composition, a test device, method of making the test device and process for determining glucose in a body fluid sample are disclosed. More particularly, the contemplated test means comprises glucose oxidase, a peroxidatively active substance, such as peroxidase, and a 3,3',5,5'-tetraalkylbenzidine indicator in an amount sufficient rapidly to produce upon contact of the test means with a predetermined amount of a glucose containing sample, a stable colored reaction product. The preferred benzidine indicator is 3,3',5,5'-tetramethylbenzidine.
1 Claim, 5 Drawing Figures
