Therapeutic inhibitor of vascular smooth muscle cells

1 -q

1 KINETICS OF NRAN01 ON A375m/m

o

O)

(/) m <

.01

~~i—i—i i i N11—i—i—i i 11111—i—\—i i 11111—i—\—i 111111 .01 .1 1 10 100

ug/ml NRAN01

.01

□ 5 MIN

♦ 15 MIN

■ 30 MIN

o 60 MIN

1 1—I I I I I I f 1 1 1—I I I I I I 1 1—I—I Mill 1 1—I I I III

.01

1 1 10

ug/ml NRAN01

100

FIG. 4B

110-1 10090-I

f£ 30 A

o a: \

I 70-J *60

50 -I

40

24 HOUR RORIDIN A-o/n RECOVERY ON B054

□ □ □ □

□ NRAN01-RA ♦ FREE RA

Mm i ni|—i ri 11 ill]—i i i 11 ui|—i -i 11 ii ri|—n rrnnj" i i i nni| 0001 .001 .01 .1 1 10 100

ug/ml OF RORIDIN A

FIG. 5A

120^ 100

_j o

% 80

8 60-]

40-^

20

24 HOUR EXPOSURE OF NRAN01-RA-o/n
RECOVERY ON B054, HT29, M14

.0001

□ B054-NRAN01-RA
♦ HT29-NRAN01-RA
■ M14-NRAN01-RA

19n 24 HOUR EXPOSURE OF NRAN01-PE-o/n lzu_1 RECOVERY ON B054, HT29, M14

11n 5 MIN. RORIDIN A, DIRECT,

~| [3H]LEUCINE-4 HOURS ON A375m/m