A pyruvate oxidase with excellent thermal stability, which is stable at pH 7.0 for 10 minutes at a temperature of up to 45.degree. C., an analytical method using said pyruvate oxidase, and an analytical reagent used for said method....http://www.google.com.au/patents/US4965194?utm_source=gb-gplus-sharePatent US4965194 - Pyruvate oxidase and an analytical method using the same