A method of determining a ligand of interest in a ligand is described. The method, which makes it possible to detect and quantify a ligand in a liquid, makes use of a biotin-avidin system which can be used to carry out immunoassays in both heterogeneous and homogeneous format. The basic components used...http://www.google.com.au/patents/US4863876?utm_source=gb-gplus-sharePatent US4863876 - Method of detecting and quantifying ligands in liquids via biotin-avidin-medicated fluorescence polarization