WO2015177811A3 - System and method for measuring permeability of drugs/toxic/ chemical compounds - Google Patents

System and method for measuring permeability of drugs/toxic/ chemical compounds Download PDF

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Publication number
WO2015177811A3
WO2015177811A3 PCT/IN2015/000217 IN2015000217W WO2015177811A3 WO 2015177811 A3 WO2015177811 A3 WO 2015177811A3 IN 2015000217 W IN2015000217 W IN 2015000217W WO 2015177811 A3 WO2015177811 A3 WO 2015177811A3
Authority
WO
WIPO (PCT)
Prior art keywords
lipid
monolayer
permeability
drugs
membrane
Prior art date
Application number
PCT/IN2015/000217
Other languages
French (fr)
Other versions
WO2015177811A2 (en
Inventor
S.Harshal
Doble Mukesh
Original Assignee
Indian Institute Of Technology Madras
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Indian Institute Of Technology Madras filed Critical Indian Institute Of Technology Madras
Priority to US15/313,626 priority Critical patent/US20170138969A1/en
Priority to GB1621320.9A priority patent/GB2541832B/en
Publication of WO2015177811A2 publication Critical patent/WO2015177811A2/en
Publication of WO2015177811A3 publication Critical patent/WO2015177811A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/08—Investigating permeability, pore-volume, or surface area of porous materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/08—Investigating permeability, pore-volume, or surface area of porous materials
    • G01N2015/086—Investigating permeability, pore-volume, or surface area of porous materials of films, membranes or pellicules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2405/00—Assays, e.g. immunoassays or enzyme assays, involving lipids
    • G01N2405/04—Phospholipids, i.e. phosphoglycerides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Abstract

An improved system and method for measuring the permeability of drugs/toxin chemical compounds utilizing phospholipid monolayer is disclosed. A phospholipid monolayer can be deposited on to a cellulose membrane by vertical lifting or Langmuir Schafer technique. The phospholipid monolayer or Langmuir Blodgett (LB) film can be coated on the cellulose membrane or porous support. Depositing the lipid onto porous support without the use of solvent and maintaining the lipid arrangement,packing similar to biological cell membrane is challenging. The lipid coated membrane support is attached to donor wells, which can be fabricated using Polystyrene tubes/ Poly acrylic tubes. The permeability of the drug/toxin/chemical compound can be further measured using the phospholipid monolayer by Boyden chamber approach. Such an improved method and system can be adapted for effective measurement of permeability of drugs/toxin chemical compounds through lipid layer in order to predict entry of such compounds into the blood stream. The current monolayer based system(LB- PAMPA) is solvent free, the lipid arrangement is similar to cell membrane and hence biomimetic, The system utilizes lipid monolayer which contains lipid molecules arranged in a definite order approaching packing in the cell membrane. The method can be adapted for mixture of lipids or pure lipids, the coating method is scalable and the entire system is High throughput and biomimetic.
PCT/IN2015/000217 2014-05-23 2015-05-22 System and method for measuring permeability of drugs/toxic/ chemical compounds WO2015177811A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US15/313,626 US20170138969A1 (en) 2014-05-23 2015-05-22 System And Method For Measuring Permeability Of Drugs/Toxic/Chemical Compounds
GB1621320.9A GB2541832B (en) 2014-05-23 2015-05-22 System and method for measuring permeability of drugs/toxic/chemical compounds

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN2550CH2014 2014-05-23
IN2550/CHE/2014 2014-05-23

Publications (2)

Publication Number Publication Date
WO2015177811A2 WO2015177811A2 (en) 2015-11-26
WO2015177811A3 true WO2015177811A3 (en) 2016-01-21

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2015/000217 WO2015177811A2 (en) 2014-05-23 2015-05-22 System and method for measuring permeability of drugs/toxic/ chemical compounds

Country Status (3)

Country Link
US (1) US20170138969A1 (en)
GB (1) GB2541832B (en)
WO (1) WO2015177811A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11293911B2 (en) 2017-12-28 2022-04-05 Korea Advanced Institute Of Science And Technology Multi-phase liquid composition, device for measuring permeability of drugs, and method for measuring permeability of drugs
CN111100789B (en) * 2018-12-24 2023-08-11 天津大学 Phospholipid membrane perforating system and method based on ultra-ultrasonic sound hole effect

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030111406A1 (en) * 2000-03-23 2003-06-19 Kiyohiko Sugano Lipid membrane, method for measuring membrane permeability, and method for screening
US20030219716A1 (en) * 2002-01-31 2003-11-27 Pion, Inc. Method and apparatus for improving in vitro measurement of membrane permeability of chemical compounds
EP1779921A1 (en) * 2005-10-27 2007-05-02 Becton, Dickinson & Company Immobilized multi-layer artificial lipid membrane for permeability measurements with the PAMPA method

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE532576T1 (en) * 2005-09-20 2011-11-15 Aquaporin As BIOMIMETIC WATER MEMBRANE USED IN GENERATING ENERGY FROM SALT GRADIENTS, COMPRISING AQUASPORINS

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030111406A1 (en) * 2000-03-23 2003-06-19 Kiyohiko Sugano Lipid membrane, method for measuring membrane permeability, and method for screening
US20030219716A1 (en) * 2002-01-31 2003-11-27 Pion, Inc. Method and apparatus for improving in vitro measurement of membrane permeability of chemical compounds
EP1779921A1 (en) * 2005-10-27 2007-05-02 Becton, Dickinson & Company Immobilized multi-layer artificial lipid membrane for permeability measurements with the PAMPA method

Also Published As

Publication number Publication date
GB201621320D0 (en) 2017-02-01
WO2015177811A2 (en) 2015-11-26
GB2541832B (en) 2020-08-19
GB2541832A (en) 2017-03-01
US20170138969A1 (en) 2017-05-18

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