WO2013183797A1

WO2013183797A1 - Regenerative cell extraction device

Info

Publication number: WO2013183797A1
Application number: PCT/KR2012/004425
Authority: WO
Inventors: 도병록; 이정규; 김지향; 박승훈; 김명진; 강성구; 김철근; 김종훈
Original assignee: 주식회사 휴림바이오셀
Priority date: 2012-06-05
Filing date: 2012-06-05
Publication date: 2013-12-12

Abstract

The present invention relates to a regenerative cell extraction device for the isolation and breakdown of tissue comprising regenerative cells. The regenerative cell extraction device according to the present invention comprises: a container which rotates upon receiving a rotational force from the outside, and is formed so as to have a space for accommodating tissue; a hollow stocking-and-releasing tube which is inserted into the container in such a way that one end thereof is disposed at the bottom end of the container and the other end thereof is disposed on the outside of the container; and a screen member which is provided on the inside of the container so as to prevent lumps of more than a predetermined size, which are formed during the process of isolating tissue, from descending to the bottom end of the container.

Description

Regenerated Cell Extractor

The present invention relates to an apparatus for extracting cells by separating tissues, and more particularly, apparatus for extracting regenerated cells such as stem cells by performing separation according to specific gravity by rotating tissues such as adipose tissue, bone marrow, and cord blood. It is about.

Stem cells are defined as cells that possess clonogenic and self-renewal ability that can differentiate into multiple cell lines under certain conditions. Embryonic stem cells are derived from mammalian embryos at the blastocyst stage and possess the ability to differentiate into almost all cells present in the body, while adult stem cells are stem cells that are present in trace amounts in differentiated tissue after birth. It is a cell with the capacity of a cell.

Adult stem cells offer practical advantages over embryonic stem cells. In other words, adult stem cells, unlike embryonic stem cells, do not cause ethical problems and can be extracted from the patient. They are inherent in various tissues of the human body and have a high supply amount. The most available sources of adult stem cells are bone marrow, peripheral blood, umbilical cord / umbilical cord blood and adipose tissue, as confirmed in recent studies. These cells can maintain, yield and replace the final differentiated cells in their own specific tissues as a result of tissue damage due to physiological cell turnover or wounds.

This ability, called cell plasticity, has led to the development of therapeutic applications aimed at regenerating defective tissues for the purpose of restoring the physiology and function of damaged organs. Adult stem cells are distributed not only in hematopoietic cells but also in various tissues, as known for decades, and as recently discovered, can be differentiated into blood vessels, muscles, bones, cartilage, skin, nerves, and the like. These cells are known as mesenchymal stem cells. In addition, platelet concentrates can be used to accelerate wound healing and, consequently, play a role in regenerative medicine that aids in the reconstitution of tissues such as bone, skin or other tissues.

Recently, adipose tissue has been found to contain large amounts of stem cells, progenitor cells and matrix materials suitable for therapeutic applications. Adipose tissue is also a rich source of vascular endothelial cells, which can contribute to tissue regeneration by promoting growth of renal blood vessels and stimulating stem and progenitor cell growth.

On the other hand, in order to extract cells from the adipose tissue, when the adipose tissue is washed in a container or separated from the adipose tissue using an enzyme, collagen is separated from the adipose tissue and agglomeration in a container occurs. I do it.

The collagen formed in this way has a problem in that it is difficult to separate the adipose tissue and extract the regenerated cells because the device is malfunctioning by closing the entrance and the tube communicating with the outside, there is a request for a solution.

SUMMARY OF THE INVENTION The present invention has been made in view of the above-described problems, and an object thereof is to provide a regenerated cell extracting device having an improved structure to prevent a malfunction of the device by closing the entrance tube by agglomerates of a predetermined size or more, such as collagen.

The regenerated cell extracting apparatus according to the present invention for achieving the above object is to extract the regenerated cells by separating the tissue (tissue), the container is formed by rotating the rotational force received from the outside and the space is accommodated tissue and formed; One end is disposed at the lower end of the container and the other end has a hollow entrance tube inserted into the container to be disposed outside the container.

In addition, a screen member is installed inside the container to prevent a mass of a predetermined size or more formed in the process of separating the tissue from falling down to the lower end of the container, and the screen member may be formed in various shapes such as a comb tooth shape.

In the regenerative cell extracting apparatus according to the present invention, the screen member is installed to prevent entry of substances that can hinder the movement of the pipe, such as collagen mass formed during separation and decomposition of tissues, into the bottom of the container. The problem of being closed by the collagen lump has been solved, thereby providing an advantage that the separation and disassembly of tissues can be performed smoothly and efficiently.

1 is a schematic isolated perspective view of a regenerated cell extracting apparatus according to a first embodiment of the present invention.

FIG. 2 is a schematic cross-sectional view taken along the line II-II of FIG. 1.

3 is a schematic cross-sectional view taken along line III-III of FIG. 2.

4 is a schematic cross-sectional view of a regenerated cell extraction apparatus according to a second embodiment of the present invention.

FIG. 5 is a schematic cross-sectional view taken along the line VV of FIG. 4.

6 is a schematic cross-sectional view of a regenerated cell extraction apparatus according to a third embodiment of the present invention.

7 is a schematic cross-sectional view taken along line VII-VII of FIG. 6.

8 is a schematic exploded perspective view illustrating a modified coupling example of the screen member and the cap in the first embodiment.

9 is a cross-sectional view of the cap and the screen member shown in FIG.

The regenerated cell extracting apparatus according to the present invention for achieving the above object is to extract the regenerated cells by separating the tissue (tissue), the container is formed by rotating the rotational force received from the outside and the space is accommodated the tissue is formed; One end is disposed at the lower end of the container and the other end has a hollow entrance tube inserted into the container to be disposed outside the container.

The screen member is provided with a plurality of locking members formed toward the outside from the center portion of the container, the plurality of locking members are arranged to be spaced apart from each other along the circumferential direction.

In addition, the plurality of locking members are disposed along the circumferential direction of the entrance pipe, the interval between the plurality of locking members may be formed in the range of 1 ~ 5mm.

In addition, the locking member is formed by bending toward the lower outer portion of the container from the upper center portion of the container, it is made of a flexible material. The lower end of the locking member is preferably supported in contact with the inner wall or the bottom of the container.

In addition, to facilitate separation of the tissue, a plurality of plate-shaped stirring blades protruding from the bottom of the container is formed, a plurality of slits that the stirring blades can be fitted to the lower end of the plurality of supporting members Is formed. However, even if there is no slit, the stirring blade may be sandwiched between the catching members, the stirring blade may not be inserted between the catching members.

On the other hand, the latching member employed in another embodiment of the present invention, the first catching portion formed upward from the bottom of the container, and the second catching portion extending from the upper end of the first catching portion to the outer side of the container Equipped.

In addition, it may be further provided with an annular first auxiliary locking member disposed surrounding the second locking portions of the plurality of locking members.

And a plurality of second auxiliary catching members disposed between the catching member and the access pipe to be spaced apart from each other along the circumferential direction of the access pipe, and formed to extend upward from the bottom surface of the container. It can be provided.

Hereinafter, with reference to the accompanying drawings, a first embodiment of the present invention will be described in more detail.

1 is a schematic exploded perspective view of a regenerated cell extracting apparatus according to a first embodiment of the present invention, FIG. 2 is a schematic cross-sectional view taken along line II-II of FIG. 1, and FIG. 3 is a schematic cross-sectional view taken along line III-III of FIG. 2.

1 to 3, the regenerated cell extracting apparatus 100 according to the first embodiment of the present invention is to rotate the tissue containing the regenerated cells, such as fat tissue (fat tissue), etc. to separate according to the specific gravity The container 10 and the entrance pipe 30 are provided.

The container 10 is used to rotate and separate adipose tissue, and the container 10 includes a bowl 11 and a cap 12 coupled to the upper portion of the bowl 11. When the ball 11 and the cap 12 are coupled, a space 13 is formed therein to accommodate adipose tissue.

The body portion of the ball 11 is formed to gradually decrease in diameter toward the lower side, but the degree of the decrease in diameter is small. Of course, the diameter does not become small and may be constant. However, the lower part of the ball 11 is formed in a shape in which the diameter rapidly decreases toward the lower side, the groove portion 14 is formed concave at the lowest end of the ball (11).

A through hole 15 is formed in the central portion of the cap 12, and a protruding wall portion 16 is formed to protrude upward from the outer circumference of the through hole 15.

In addition, the stirring blade 17 is formed on the inner wall of the lower portion of the ball (11). The stirring blade 17 is formed in a plate shape and protrudes from the inner wall toward the space 13. Four stirring blades 17 are arranged symmetrically at intervals of 90 ° with respect to the center of rotation of the container 10. Of course, there may be only one stirring blade 17, although not necessarily symmetrical, it is preferable that the symmetrical arrangement. Stirring blade 17 is rotated together in accordance with the rotation of the vessel 10, serves to smoothly stir adipose tissue.

The entrance tube 30 functions as a flow path for transferring the washing liquid, the enzyme, or the like to the space 13 of the container 10 or for discharging the substance in the container 10 to the outside. The entrance tube 30 is formed in a hollow shape and is inserted into the container 10 through the through hole 15 of the container 10. The lower end of the entrance pipe 30 is disposed in the groove 14 of the container 10 in a state of being close to the bottom of the container 10, and the upper end of the entrance pipe 30 is disposed above the container 10.

In addition, in the present embodiment, an auxiliary pipe 40 is installed to communicate the inside and the outside of the container 10 in an independent path from the access pipe 30. The auxiliary pipe 40 functions as a passage through which air flows into and out of the container 10. Therefore, the auxiliary pipe 40 does not need to be inserted to the lower end of the container 10, it is sufficient that only the upper end of the container 10 is inserted.

The entrance tube 30 and the auxiliary tube 40 may be formed separately, but are integrally formed in this embodiment. That is, the entrance tube 30 is disposed long in the vertical direction and the upper portion is formed to be bent, the auxiliary tube 40 is formed to a larger diameter than the entrance tube 30 is wrapped around the entrance tube 30. The upper part of the auxiliary pipe 40 is formed by bending in the direction opposite to the direction in which the entry pipe 30 is bent. The interior of the auxiliary pipe 40, more precisely, the space between the inner peripheral surface of the auxiliary pipe 40 and the outer peripheral surface of the entrance pipe 30 forms a flow path 45 through which air can flow. However, the passage 45 is not necessarily used only for the inflow and outflow of air, and may be used as the inflow and outflow path of various materials, such as to discharge substances such as blood contaminants or inject a washing solution, if necessary.

In addition, an insertion hole 35 is formed at an upper end of the entrance pipe 30, and a stopper 36 is screwed to the insertion hole 35. When the stopper 36 is removed, a syringe needle (not shown) may be inserted into the entrance tube 30 through the insertion hole 35 to suck the liquid substance placed in the groove 14 of the container 10. When the syringe needle is not used, the insertion hole 35 is closed by the stopper 36 to prevent contamination of the space 13 inside the container 10. In addition to the syringe needle, a tube may be inserted into the insertion hole 35 to exchange materials with the inside of the container 10.

And, the lower side of the auxiliary pipe 40, the shielding member 47 is fitted to the entrance pipe 30 is coupled. When the container 10 is rotated to separate the adipose tissue, a liquid substance such as blood or washing liquid may be rapidly raised to the upper part of the container 10, and these materials may be inside or in the auxiliary tube 40. It may be drawn between the 40 and the inner circumferential surface of the through hole 15 of the container 10. The shielding member 47 surrounds the through hole 15 and the auxiliary pipe 40 to prevent blood contaminants from being introduced into the auxiliary pipe 40 or the like. However, the auxiliary pipe 40 is to be through the space 13, so that the wrapping here is not to be completely sealed, the gap (d) is formed in the upper portion to communicate with the space (13).

In addition, spiral outer auxiliary stirring blades (not shown) are formed on the outer circumferential surface of the entrance tube 30, and the adipose tissue may be stirred together with the stirring blade 17 at the lower side of the container 10.

Meanwhile, the container 10 should be rotated relative to the entrance pipe 30 and the auxiliary pipe 40, and the space 13 of the container 10 is sealed between the auxiliary pipe 40 and the container 10. Should be sealed.

In this embodiment, the compressive sealing member 50 and the friction member 60 are employed, so that the space 13 is closed while the container 10 is rotated. That is, the sealing member 50 employed in the present embodiment is formed in an annular shape and fitted to the protruding wall portion 16 of the cap 12, and the flange portion 48 and the cap (formed on the outer circumferential surface of the auxiliary pipe 40) 12) is interposed between. Sealing member 50 is made of an elastically compressible rubber material, when the compression between the flange portion 48 and the cap 12 of the auxiliary pipe 40, the gap between the through-hole 15 and the auxiliary pipe 40 Is completely sealable.

However, when the sealing member 50 is rotated together with the container 10, the rubber sealing member 50 has a high coefficient of friction and thus does not smoothly rotate due to friction between the flange portion 48 and high heat of friction. This happens. The upper side of the sealing member 50 is attached to the friction member 60 of the material having a low coefficient of friction and high heat transfer coefficient. In this embodiment, the friction member 60 made of a ceramic material and formed in an annular shape is attached to the upper portion of the sealing member 50, and the friction member 60 is in close contact with the flange portion 48 of the auxiliary pipe 40. Surface contact.

However, in another embodiment, a retaining ring (not shown) and a bearing (not shown) may be used to achieve relative rotation and sealing between the auxiliary pipe 40 and the container 10.

In addition, in the present embodiment, since the auxiliary pipe 40 has a structure in which the entrance pipe 30 and the auxiliary pipe 40 are integrally formed in a form surrounding the entrance pipe 30, the outer circumferential surface of the auxiliary pipe 40 and the through hole ( Although sealing between 15) has been a problem, in other embodiments the auxiliary pipe 40 may or may not be formed separately from the access pipe 30. Even in this embodiment, the entrance pipe 30 is fitted in the through hole 15, in order to ensure the sealing and relative rotation between the entrance pipe 30 and the through hole 15, on the outer peripheral surface of the entrance pipe 30 A flange portion (not shown) is formed, and the sealing member 50 and the friction member 60 may be provided between the flange portion (not shown) and the cap 12 formed on the outer circumferential surface of the access pipe 30.

On the other hand, the access pipe 30 is connected to the pump (not shown) by a connecting tube (not shown), to provide a substance such as enzyme, washing liquid, etc. to the container 10 through the access pipe 30, or the container ( 10) Discharge fat cells or waste fluid to the outside. In this embodiment, since a material such as a washing solution should be introduced into and out of the container 10, the pump should provide suction in both directions. The pump uses a peristaltic pump that rotates in both directions to provide suction.

In the regenerative cell extracting apparatus 100 having the above-described configuration, after the adipose tissue and the washing solution are added, the container 10 is rotated to wash the adipose tissue, or the collagenase enzyme is added to decompose the adipose tissue. ), Collagen is released from adipose tissue. The collagen discharged as described above may form a large mass while agglomerating with each other, and when the collagen mass enters the groove 14 of the container 10, the entrance tube 30 may be closed.

In the present invention, the collagen lump is provided with

screen members

70, 80, and 90 to prevent the entrance tube 30 from closing.

The screen member 70 employed in the first embodiment has a supporting member 71 and a plurality of locking members 72. The support member 71 is formed in an annular shape to surround the entrance pipe 30 and the shielding member 47 and is coupled to the bottom surface of the cap 12. The plurality of locking members 72 extend downward from the lower end of the support member 71 and are bent to the outside of the container 10 to be in contact with the bottom or inner surface of the container 10.

As shown in FIG. 3, when the plurality of catching members 72 are viewed from above, the catching members 72 are formed outwardly from the center of the container 10, and the circumferential direction (more specifically, Spaced apart from each other along the circumferential direction) so as to be disposed radially as a whole.

The interval between the plurality of catching members 72 is formed in a range of approximately 1 to 5 mm, so that the collagen mass is not discharged through the catching members 72. However, the above interval may vary in accordance with the type of tissue.

In addition, a slit 73 is formed at the lower end of the locking member 72 disposed at a position corresponding to the stirring blades 17 of the locking members 72. The slit 73 is fitted with a plate-shaped stirring blade 17 is engaged.

The locking member 72 is made of a flexible material to bend in the vertical direction.

Hereinafter, a process of separating the adipose tissue by the regenerated cell extracting apparatus 100 having the above-described configuration will be described.

First, the adipose tissue to be separated is introduced into the regenerated cell extracting apparatus 100 through the entrance tube 30. For example, after pre-extracting adipose tissue from the human body through a syringe, the adipose tissue may be introduced into the extraction apparatus 100 again. However, in order to prevent contamination during the transfer process, the syringe needle is directly plugged into the human body and a tube connected to the syringe syringe is connected to the entrance tube 30, and the inside of the container 10 using a pump (not shown). Inducing a negative pressure in the adipose tissue can be introduced directly into the container 10 directly from the human body.

As described above, when adipose tissue flows into the container 10, a pump (not shown) is operated to transfer the washing liquid to the container 10. The rotary jig (not shown) is operated to repeat the rotation and stop with respect to the container (10). When the rotation and stop are repeated at regular intervals, the adipose tissue and the washing solution are centrifuged while mutually stirring, and the adipose tissue introduced with the blood-derived contaminants is washed.

When the container is rotated in this way, the adipose tissue and the washing liquid are rotated while being in close contact with the inner wall of the container 10, and the water level is increased. It is pushed up to the upper side of the container 10 between the inner wall of the (10) or between the locking member (72).

After stopping the vessel 10 for about one minute, the materials that have risen along the inner wall of the vessel are lowered to the lower portion of the vessel 10 and separated according to specific gravity. That is, heavy components such as washing liquid, blood, and body fluid are disposed in the lower part of the container 10, and washed fatty tissue is disposed on these upper parts, and the oil component is disposed on the uppermost layer.

However, the collagen, which has been separated from the adipose tissue and formed a lump of a predetermined size, does not come down to the bottom of the container 10 by being caught by the locking member 72 of the screen member even after the rotation of the container 10 is stopped. In particular, since the locking members 72 are supported on the inner surface or the bottom surface of the container 10, even if the locking member 72 is made of a flexible material, the collagen mass may push out the locking member 72 and flow downward. Can't. In addition, even if the locking member 72 is not supported on the inner surface or the bottom surface of the container 10, it maintains a resistance that does not bend by the weight of the collagen mass. Therefore, the collagen lump does not occur in the bottom of the container 10, in particular, the groove 14 to close the entrance pipe 30.

Meanwhile, after discharging blood contaminants in the above state, the collagenase enzyme is introduced through the entrance tube 30, and the container 10 is rotated again for about 30 minutes. Collagenase decomposes adipose tissue, and the decomposed components are separated by the rotation of the container 10. When the rotation is stopped and allowed to stand for about 1 minute, the aqueous solution containing the adipose-derived stem cells is a relatively heavy component. (10) is placed below, on which mature fat cells (matured fat cells) and oil components are placed. Aqueous solutions containing adipose derived stem cells are discharged for use in subsequent processes.

In the process of decomposing adipose tissue by the enzyme, if the collagen decomposed from the adipose tissue agglomerates with each other to form a large mass, the mass is caught by the catching member 72 of the screen member 70 even if the rotation of the vessel is stopped. 10) can not come down to the bottom. Therefore, the collagen lump does not occur in the bottom of the container 10, in particular, the groove 14 to close the entrance pipe 30.

So far, the screen member 70 has been described as being simply coupled to the cap 12, but in another variation, the screen member 70 may be configured to be fitted to the cap 12. Such a modification is shown in FIGS. 8 and 9.

FIG. 8 is a schematic exploded perspective view illustrating a modified coupling example of the screen member and the cap in the first embodiment, and FIG. 9 is a cross-sectional view of the screen member and the cap shown in FIG.

8 and 9, the insertion portion 19 is concave in the center portion of the cap 12, and the through hole 15 is provided in the insertion portion 19. Of course, the protruding wall portion 16 is formed upward from the outer circumference of the through hole 15. The support member 71 of the screen member 70 is coupled by being fitted to the fitting portion 19 of the cap 12. The cutout 19 is formed above the support member 71. The cutout 19 is for allowing air to flow smoothly between the inside and the outside of the screen member 70, and a plurality of cutouts 19 are formed in this embodiment. Even when the screen member 70 is fitted to the fitting portion 19 of the cap 12, a portion of the lower portion of the cutout 79 is exposed to the lower portion of the fitting portion 19, so that the inner side of the screen member 70 may be exposed. Air can flow between the outside. In addition, a through hole is formed in the support member even if the cutout portion is not formed, so that air can flow by communicating between the inside and the outside of the screen member.

Although the first embodiment has been described so far, the configuration of the screen member is not limited to the screen member 70 employed in the first embodiment, and various forms are possible. Hereinafter,

screen members

80 and 90 employed in the second and third embodiments will be described.

In the regenerative cell extracting apparatus 200 according to the second embodiment and the regenerative cell extracting apparatus 300 according to the third embodiment, the container 10, the access pipe 30, the auxiliary pipe 40, and the sealing member 50 are provided. And all components, such as the friction member 60 is the same as in the first embodiment, so a description thereof will be omitted, and only the screen members (80,90) will be described.

4 is a schematic cross-sectional view of a regenerated cell extraction apparatus according to a second embodiment of the present invention, Figure 5 is a schematic cross-sectional view of the V-V line of FIG.

4 and 5, the screen member 80 employed in the regenerative cell extracting apparatus 200 according to the second embodiment is installed on the inclined bottom surface of the container 10. More specifically, the screen member 80 includes a first catching portion 81 and a second catching portion 82.

The first catching portion 81 is formed upward from the inclined bottom surface of the container 10, and is slightly bent to the outside of the container 10. The second catching portion 82 extends downward from the top end of the first catching portion 81 but is slightly bent outwardly of the container 10, such that the screen member 80 is the same as a shark fin when viewed from the side. To form.

In addition, as shown in FIG. 5, when the screen member 80 is viewed from the top to the bottom, the screen members 80 extend radially outward from the center of the container with respect to the entrance tube 30.

In addition, the first locking portion 81 and the second locking portion 82 may be blocked so that the screen member 80 may be formed in the form of a plate-shaped shark fin as a whole, or may be drilled therebetween.

The screen member 80 having the above-described configuration is disposed in a plurality of spaced apart from each other along the circumferential direction of the container 10, the interval between the screen member 80 is set to about 1 ~ 5mm, but such a gap is various Can be variable. Since the above intervals take into account the size of the collagen mass, the size of the collagen mass may vary depending on the tissue to be separated or the process conditions, so that the interval between the screen members may vary.

Meanwhile, in the second embodiment, the screen member 80 includes a first auxiliary catching member 83 to surround the second catching portions 82. The first auxiliary locking member 83 is formed in an annular shape and is in close contact with the second locking portion 82. Since the collagen lump is pushed toward the inner wall of the container 10 in the process of decomposing adipose tissue, when the rotation of the container 10 is stopped, it is likely to be caught by the second catching portion 82 of the screen member 80. Accordingly, the first auxiliary catching member 83 formed along the circumferential direction is installed in the plurality of second catching portions 82 formed in the radial direction from the center of the container 10 to the outer side, so that the screen member 80 is more densely formed. By being woven, it prevents the collagen mass from falling to the bottom of the container 10. In the present exemplary embodiment, two first auxiliary catching members 83 are provided, but the number thereof may vary.

The third embodiment will be described with reference to FIGS. 6 and 7. FIG. 6 is a schematic cross-sectional view of a regenerated cell extracting apparatus according to a third embodiment of the present invention, and FIG. 7 is a schematic cross-sectional view taken along line VII-VII of FIG. 6.

6 and 7, a plurality of screen members 90 employed in the third embodiment are disposed along the circumferential direction of the entrance pipe 30, and each screen member 90 is formed at the bottom of the container 10. It is formed upward from cotton and consists of flexible material. The spacing between the screen members 90 is in the range of 1 to 5 mm as in the first and second embodiments, and this spacing can be varied.

The screen member 90 according to the third embodiment also has the collagen agglomerates agglomerated in the form of agglomerates in the process of separating and disintegrating adipose tissue. It does not flow into the bottom, in particular the groove 14. In this way, the collagen lump can be prevented from blocking the entrance pipe 30.

Meanwhile, the screen member 90 employed in the third embodiment and the screen member 80 employed in the second embodiment may be employed together. In this embodiment, the screen member employed in the third embodiment 300 may be employed. 90 may be installed between the screen member 80 and the entrance pipe 30 employed in the second embodiment (200).

As described above, in the regenerated cell extracting apparatus (100, 200, 300), the collagen in the form of agglomerate formed in the process of separating and decomposing adipose tissue flows into the bottom of the container 10, in particular the groove 14, the entrance tube 30 is In order to prevent closure,

screen members

70, 80, and 90 are installed to block the movement of collagen between the upper and lower sides of the container 10.

Although the shape and structure of the

screen members

70, 80, and 90 are various, the plurality of members are arranged to be spaced apart from each other at narrow intervals so that collagen may be caught on the screen member.

And the collagen is prevented from closing the entrance tube by the screen member of the above configuration, the regenerated cell extractor can smoothly perform the separation and degradation of the tissue containing the regenerated cells.

And it has been described that the screen member is prevented from coming down to the bottom of the container is a collagen lump, but is not necessarily limited to the collagen lump, the material formed into a lump enough to block the entrance tube is also filtered by the screen member Also note that it cannot come down to the bottom of the container.

As described above, in the detailed description of the present invention has been described with respect to preferred embodiments of the present invention, those skilled in the art to which the present invention pertains various modifications can be made without departing from the scope of the present invention Of course.

Therefore, the scope of the present invention should not be limited to the described embodiments, but should be determined not only by the claims below, but also by the equivalents of the claims.

Claims

To separate the tissue (tissue) to extract regenerated cells,

A container in which a space portion for receiving tissue is rotated and a tissue is received;

A hollow entrance tube having one end disposed at a lower end of the container and the other end inserted into the container so as to be disposed outside the container; And

And a screen member installed inside the container so as to prevent a mass of a predetermined size or more formed in the process of separating the tissue from descending to the lower end of the container.
The method of claim 1,

The screen member is provided with a plurality of locking members formed toward the outside from the center of the container, the plurality of locking members are regenerated cell extraction apparatus, characterized in that arranged in the circumferential direction spaced apart from each other.
The method of claim 2,

The plurality of locking members are regenerated cell extraction device, characterized in that disposed along the circumferential direction of the entrance tube.
The method of claim 2,

The spacing between the plurality of engaging members is regenerated cell extraction apparatus, characterized in that formed in the range of 1 ~ 5mm.
The method according to claim 1 or 2,

The locking member,

Regenerated cell extraction device, characterized in that formed by bending from the upper center of the container toward the lower outer portion of the container, made of a flexible material.
The method of claim 5,

The lower end of the locking member is in contact with the inner wall or the bottom surface of the container is regenerated cell extraction apparatus, characterized in that supported.
The method of claim 5,

The upper portion of the container is coupled to the annular support member surrounding the entry pipe,

Regenerated cell extracting apparatus, characterized in that each upper end of the plurality of engaging members is fixed to the support member.
The method of claim 5,

In order to facilitate the separation of the tissue, a plurality of plate-shaped stirring wings protruding from the bottom of the container is formed,

Regenerated cell extraction device, characterized in that the lower end of the portion of the plurality of supporting members is formed with a slit into which the stirring blade can be fitted.
The method of claim 5,

The upper portion of the container is formed with fittings,

The support member of the screen member is regenerated cell extraction device, characterized in that coupled to the fitting portion.
The method of claim 9,

The support member is provided with a through hole or cutout for communicating between the inside and the outside of the screen member,

The through-holes or incisions are regenerated cell extraction apparatus, characterized in that exposed even when the support member is fitted to the fitting portion.
The method of claim 1,

The screen member has a regenerated cell extraction apparatus having a first catching portion formed upward from the bottom of the container, and a second catching portion extending downward from the upper end of the container from the upper end of the first catching portion.
The method of claim 11,

Regenerating cell extracting apparatus, characterized in that it further comprises an annular first auxiliary locking member disposed surrounding the second locking portions of the plurality of screen members.
The method of claim 11,

A plurality of second auxiliary catching members are disposed between the screen member and the entrance tube to be spaced apart from each other along the circumferential direction of the entrance tube, and are formed of a flexible material and extend upward from the bottom surface of the container. Regenerated cell extracting device, characterized in that.
The method of claim 1,

And a plurality of screen members disposed to be spaced apart from each other along the circumferential direction of the entrance tube and formed of a flexible material so as to extend upward from the bottom surface of the container.