WO2013107075A1

WO2013107075A1 - Infusion set for producing agarwood and production method of agarwood

Info

Publication number: WO2013107075A1
Application number: PCT/CN2012/071599
Authority: WO
Inventors: 魏建和; 张争; 杨云; 孟慧; 高志晖; 徐艳红; 张兴丽; 刘洋洋; 陈旭玉; 陈伟平; 冯锦东; 陈怀琼
Original assignee: 中国医学科学院药用植物研究所; 中国医学科学院药用植物研究所海南分所
Priority date: 2012-01-20
Filing date: 2012-02-24
Publication date: 2013-07-25
Also published as: MY186014A; CN102577856A

Abstract

An infusion set comprises a liquid storage container (3) and an infusion tube (17). The liquid storage container (3) has a bottom outlet and is used for containing inducing liquid for agarwood. The infusion tube (17) is connected to the liquid storage container (3), and is used for delivering the inducing liquid for agarwood from the liquid storage container (3) to the trunk of the tree. The infusion set conveniently and precisely delivers the inducing liquid for agarwood to the trunk, the top bough, and the lateral branch of the tree, so that the agarwood can be produced all over the tree. The infusion set can be applied to trees of Aquilaria sinensis with different ages and diameters at breast height to produce the agarwood, hence overcoming the shortages of rot, death and the like due to the large-area bark and trunk injuries that are easily incurred by fast infusion and failure of precise quantitation and control, and reducing the tree death rate.

Description

Infusion device for producing agarwood and method for producing agarwood

Technical field

The invention belongs to the technical field of agarwood production, and in particular relates to an infusion device for producing agarwood and a method for producing agarwood. Background technique

Agarwood is a traditional precious medicinal herb and valuable natural spice in China, Japan, India and other Southeast Asian countries. The healthy agarwood tree can not produce agarwood. The tree body only begins to be scented when it is damaged by physical damage, chemical damage and fungal infection. , eventually the formation of agarwood.

A device for inducing agarwood infection by inducing injury signal and salt ion injury stress, which uses infusion method to induce injury signal induction and salt ion into plant transduction tissue to achieve injury-induced induction of white woody Agarwood. The shortcoming of this technology is that a large amount of high-intensity damage-inducing liquid is directly input into plant tissues, resulting in a large input and rapid release of the damage-inducing liquid, which is likely to cause damage to large-area bark and tree trunks, leading to rot, and even death of the entire tree. However, reducing the concentration of the injury-inducing liquid can not achieve the purpose of harm-induced white woody aroma, and the technical defect hinders the large-scale popularization and application of agarwood production to a certain extent. Summary of the invention

SUMMARY OF THE INVENTION The object of the present invention is to provide an infusion device for producing agarwood which is capable of ingeniously and accurately transporting the agarwood-inducing liquid to the trunk of the tree, the top main technique and the side technique, thereby realizing the whole body aroma of the tree body.

The infusion device of the present invention comprises a liquid storage container having a bottom outlet and for containing agarwood inducing liquid, and the infusion tube is connected to the liquid storage container and used for inputting the agarwood inducing liquid from the liquid storage container to the trunk of the tree in.

A slow release core is preferably provided at the bottom outlet of the liquid storage container. The sustained release core can be placed in a microporous housing.

Preferably, a graduated burette is further disposed between the liquid storage container and the infusion tube, and the infusion tube passes The burette is in communication with the reservoir. The upper end of the burette may be provided with an air inlet and the lower end may be provided with a throttle valve. The burette is preferably in communication with the bottom outlet of the reservoir via a draft tube. A rubber stopper for preventing leakage of the induced liquid may be provided between the draft tube and the bottom outlet of the liquid container.

Preferably, the infusion tube is also connected to a pressure pump to provide pressure to the inducing fluid in the infusion tube.

A filter can also be provided in the infusion tube.

The burette can be connected to the infusion tube through a dripper and a filter is provided in the drip chamber. A flow regulator and/or a trimmer are also preferably provided on the infusion tube.

Preferably, a needle for insertion into the trunk is provided at the lower end of the infusion tube. The tip of the needle is preferably closed and has one or more holes in the side. The pore diameter is preferably about 15 μm.

The reservoir container is also preferably connected to a stopper puncturing device. One or more switch clamps can also be provided on the stopper puncturing device.

The present invention also provides a method of producing agarwood on trees capable of producing agarwood, comprising delivering the agarwood inducing liquid into trees using the aforementioned infusion device.

Preferably, the method comprises: drilling from above and below about 45 degrees from the trunk of the tree and allowing the hole to be deeper than 1/2 of the diameter of the tree; transporting the agarwood inducing fluid into the borehole using an infusion device; and sealing the borehole.

Preferably, the agarwood inducing solution is to mix a 1% phytohormone solution with a 0.8% chemical solution in a ratio of 1:20 to 1:50, the chemical solution is a potassium chloride solution, and the plant hormone is a plant injury signal. The tree may include a fragrant agarwood All tree species.

The device of the invention inputs the agarwood inducing liquid into the trunk through the infusion tube, thereby producing agarwood, and the device can be applied to the agarwood trees of various ages and DBHs to produce agarwood, which overcomes the infusion speed and cannot be accurately quantified and controlled. It is easy to cause damage to the bark and trunk and cause rot and death, which greatly reduces the mortality of trees. The device can be used repeatedly, and is easy to mass-produce, which is convenient for large-scale promotion. The method utilizes an infusion device to deliver agarwood inducing liquid to trees, increase the content of phytohormone, reduce the content and concentration of chemical solution, and achieve various induction modes and continuous stimulation by adding microbial cells, microbial cell components or metabolites. Plant cells produce damage signals, which in turn activate agarose sesquiterpenes and 2- ( 2- Ethyl) ketone anabolic accumulation produces a resin to form agarwood, thus achieving agarwood

, continuous, stable, fast, efficient, and agarwood. DRAWINGS

Figure 1 is a schematic view of the overall structure of the present invention;

2 is a schematic structural view of an embodiment of the present invention;

Figure 3 is a partial structural view of Figure 2;

Figure 4 is a schematic view of the needle structure of Figure 2.

detailed description

Specific embodiments of the present invention will be further described in detail below with reference to the drawings and embodiments. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

1 is a schematic view of the overall structure of the present invention, the infusion device for producing agarwood includes a liquid storage container 3 and an infusion tube 17, and the lower end of the liquid storage container 3 is connected with an infusion tube 17, the liquid storage The container 3 is for holding agarwood inducing liquid, and the agarwood inducing liquid is introduced into the trunk through the infusion tube 17. The liquid container 3 of the present invention may be made of metal, wood, bamboo, plastic, glass material or other material which makes the liquid non-leakable. The liquid container may be any suitable container, and may be a closed, open or open container as long as it can hold the agarwood inducing solution. The device of the invention inputs the agarwood inducing liquid into the trunk through the infusion tube, thereby producing agarwood, and the device can be applied to agarwood trees of various ages and DBHs to produce agarwood, and the trees producing agarwood include all the genus Species, and 4 genera of A. sylvestris (Aetoxylo

Gyrinops, Phaleria and Gonystylus) are all tree species that produce agarwood.

As shown in FIG. 2, a schematic structural view of an embodiment of the present invention, in order to slow the flow rate of the inducing liquid, preferably, the outlet end of the liquid storage container 3 is provided with a sustained release core 5, and the sustained release core can be variously suitable. The material is, for example, made of a polymer resin. In order to facilitate introduction of the agarwood inducing liquid into the liquid storage container, one or more stopper punctors 1 are attached to the liquid storage container 3 (to facilitate the penetration of the induction liquid supply source), and one or the bottle stopper puncturing device 1 is provided Multiple switch clamps 2. In order to facilitate the observation of the flow rate of the inducing liquid, a graduated burette is further provided between the liquid storage container 3 and the infusion tube 17. 9 , the upper end of the burette 9 is connected with the liquid storage container 3 , the lower end is connected with the infusion tube 17 , the upper end of the burette 9 is provided with an air inlet 8 , and the lower end of the burette 9 is provided with a throttle valve 10 for starting and closing Liquid flow.

As shown in FIG. 3, it is a partial structural diagram of FIG. 2, and the outlet end (bottom outlet) of the liquid storage container 3 is connected to the burette 9 through the draft tube 7, so that the inducing liquid flows into the burette 9 and is guided. A rubber stopper 6 is disposed between the flow tube 7 and the outlet end of the liquid storage container 3 to prevent leakage of the induced liquid, and the microporous housing 4 is disposed outside the sustained release core 5 (ie, the sustained release core 5 is placed in the microporous hole). In the housing 4), thereby further reducing the flow of liquid.

In order to allow the inducing liquid to have sufficient pressure to continuously flow into the tree, preferably, the infusion tube 17 is connected with a pressure pump 13 to supply pressure to the inducing liquid through the pressure pump 13, and when the transpiration in the tree is insufficient, the pressure pump 13 can provide pressure to the induction fluid. Further, the burette 9 can also communicate with the infusion tube 17 through the drip chamber 11. In order to prevent foreign matter particles from entering the tree to block the transporting tissue, a filter 12 (shown in the drip chamber 11) may be provided in the infusion tube 17. The lower end of the infusion tube 17 is also provided with a needle 16 for easy insertion into the tree. As shown in Fig. 4, the needle tip is sealed with a tip end, the side is porous, and the single hole design is also possible. The liquid filter 12 can also be located at other positions, optimally at the input end of the needle 16, and the pore size of the liquid filter 12 is generally 15 μm. In order to achieve slow and quantitative injection of the agarwood inducing liquid into the agarwood tree tissue, preferably, the infusion tube 17 is provided with a flow regulator 14 and a trimmer 15 for regulating the flow rate of the infusion tube, and the trimmer is used for fine adjustment. The flow, regulator 14 and trimmer 15 can be placed at any suitable location on the infusion tube 17, preferably with the lower end of the infusion tube 17, to facilitate adjustment control.

The present invention also provides a method for producing agarwood, which utilizes an infusion device for producing agarwood, comprising the steps of: using an infusion device to deliver one or more solutions capable of inducing agarwood to a tree that produces agarwood, at intervals of 1~ Apply once every 3 months, and form agarwood after 6~12 months of treatment. The solution used to induce agarwood used in the method includes a chemical solution, a microbial cell, a microbial cell component or a metabolite, and all solutions capable of inducing agarism. The infusion set includes all types of infusion sets and injector devices capable of inputting a solution that induces agarwood to the tree by external action such as gravity, pressure, transpiration, and the like. The lure The solution that produces agarwood includes one or more of a chemical solution, a plant hormone, a microbial cell, a microbial cell component, or a metabolite. Preferably, the solution for inducing agarwood is a mixture of a 1% phytohormone solution and a 0.8% chemical solution in a ratio of 1:20 to 1:50, wherein the chemical solution is a potassium chloride solution, and the phytohormone is Plant damage signal ethylene.

The agarwood-producing trees used in the present invention include all of the genus Asteraceae, and all four species of A. sinensis (Aetoxylon, Gyrinops, Phaleria, and Gonystylus) capable of producing agarwood. Preferably, the tree species producing the agarwood tree is > 3 years, and the DBH > 4 cm. The method for producing agarwood of the present invention utilizes plant transpiration to transport one or several solutions capable of inducing agarwood to the agarwood trunk and the sub-technique through an infusion device, and generate a damage signal through various induction modes and continuous stimulation of plant cells. (such as jasmonic acid, ethylene, etc.), and then start the agarose sesquiterpene and 2-(2-phenethyl) ketone anabolic accumulation to produce a resin to form agarwood, thereby achieving abundance, persistence, stability, rapidity, Efficient, whole body produces agarwood. The alcohol soluble content of the 6-month agarwood sample produced by the method of the present invention is higher than the 10% standard required by the Chinese Pharmacopoeia (2010 edition), and the thin-layer fingerprint data shows that the method produces 6-month agarwood and wild agarwood. There are common spots, the main components are not significantly different, and the medicinal requirements are met. This method will greatly increase the agarwood yield and shorten the agarwood production cycle.

The process for producing agarwood is described below in connection with an embodiment of the invention:

Example 1: Select a white wood incense tree with a tree age of 3 years and a breast diameter > 4 cm. Use a woodworking drill to drill 45 degrees from the tree trunk from the top to the bottom. The diameter of the drill is 0.5 cm, and the hole depth is more than 1/2 of the diameter of the tree. good. A solution capable of inducing agarwood (referred to as agarwood inducing solution, such as a chemical solution, a microbial cell, or a microbial cell component or a metabolite) is applied by means of a tree infusion to the borehole, and a high-efficiency agarwood inducing solution is applied (after application) The rubber stopper seals the hole to prevent the agarwood inducing liquid from flowing out and the rain is washed away. It is applied once or more times at intervals of 3 months. Use the tree to grow the cone and collect the inner agarwood layer of the white wood fragrant tree. Observe the thickness of the brown agarwood layer and the amount of the xylem conduit filler under the microscope, and preliminarily determine the white woody aroma effect. After artificially inducing the incense for 6 months, the resin-containing wood was cut to remove the resin-free portion, and the agarwood was dried after drying.

Example 2: Using the infusion device to damage the white wood incense and induce the incense for 6 months, the whole tree is felled, the resin-containing wood is cut, the resin-free part is removed, the dryness is dried, and the sedimentation is measured. Fragrance production. According to the Chinese Pharmacopoeia (2010 edition), an appendix XA extract method was used to prepare the agarwood oil, and the alcohol-soluble extract content of the obtained agarwood sample was determined. The sample was pulverized, passed through a No. 2 sieve, and mixed well. Take about 2g of the test sample, place it in a 250ml Erlenmeyer flask, precisely add 50ml of 95% ethanol, plug it tightly, weigh it, let it stand for 1h, connect it to the reflux condenser, heat it to a slight boiling, and keep it slightly boiling for lh. After cooling, remove the conical flask, close the stopper, weigh the weight, make up the lost weight with ethanol, shake it, and filter it with a dry filter. Precisely measure 25ml of the filtrate, dry it in a constant weight evaporating dish, evaporate it on a water bath, dry it at 105 °C for 3 h, transfer to a desiccator, cool for 30 min, quickly and accurately weigh the weight, calculate with dry product. The content (%) of the water-soluble extract in the test article. The content of alcohol extracts from three samples of agarwood was randomly determined. The same batch of samples was tested in parallel for 5 times. The content of alcohol-soluble extracts of the agarwood samples produced by the experiment was >10%, reaching the level required by the pharmacopoeia. The resulting data is as follows:

Alcohol soluble extract

Infusion 1 2952.17±2 23344..2244 1133..1155±±00..9999 Infusion 2 2637.60±1229.37 15.30±2.47 Infusion 3 3161.33±532.32 18.08±5.37 Infusion 4 4151.42±1316.71 13.91±3.36 Infusion 5 4176.62±1671.95 11.69± 2.11 Infusion 6 4229.07±1119.51 17.05±2.85 Infusion 7 4581.44±626.77 15.74±1.28 Infusion 8 4704.08±1677.31 12.35±1.68 Infusion 9 5860.74±1060.51 13.54±3.21 Semi-broken rod 120±17.79 7.61±1.25

CK 0 3.18±1.06 In the table: Semi-breaking rod and CK are the existing methods for producing agarwood

It can be seen from the above embodiment that the embodiment of the present invention inputs the agarwood inducing liquid into the trunk through the infusion tube, thereby producing agarwood, and the device can be applied to the agarwood in various tree ages and DBHs to produce agarwood, overcoming The infusion speed is fast and cannot be accurately quantified and controlled. It is easy to cause damage to the bark and trunk and cause rot and death, which greatly reduces the mortality of trees. The device can be used repeatedly, and is easy to mass-produce, which is convenient for large-scale promotion. The method utilizes an infusion device to deliver agarwood inducing liquid to trees, and generates a damage signal through various induction modes and continuous stimulation of plant cells, thereby starting the synthesis of metabolic synthesis of agarose sesquiterpene and 2-(2-phenethyl) chromone. The formation of agarwood, thereby achieving the diversity, persistence, stability, rapidness, high efficiency of the white wood incense, and the production of agarwood.

The above description is only a preferred embodiment of the present invention, and it should be noted that those skilled in the art can make several improvements and substitutions without departing from the technical principles of the present invention. It should also be considered as the scope of protection of the present invention.

Claims

Rights request

An infusion device for use in a tree capable of producing agarwood to promote the production of agarwood in the tree, comprising a liquid storage container having a bottom outlet and for containing agarwood inducing liquid, The infusion tube is in communication with the reservoir and is used to input the agarwood inducing fluid from the reservoir into the trunk of the tree.

2. Apparatus according to claim 1 wherein the reservoir outlet has a sustained release at the bottom outlet.

3. Apparatus according to claim 2 wherein the slow release core is placed in the microporous housing.

4. Apparatus according to any of claims 1-3, wherein a graduated burette is provided between the reservoir and the infusion tube, the infusion tube being in communication with the reservoir via the burette.

The apparatus of claim 4, wherein the upper end of the burette is provided with an air inlet and the lower end is provided with a throttle valve.

6. Apparatus according to claim 4 wherein the burette is in communication with the bottom outlet of the reservoir via a draft tube.

7. Apparatus according to claim 6 wherein a rubber plug is provided between the draft tube and the bottom outlet of the reservoir to prevent induced fluid leakage.

8. Apparatus according to any one of the preceding claims wherein the infusion tube is also connected to a pressure pump to provide pressure to the inducing fluid in the infusion tube.

9. Apparatus according to any one of the preceding claims wherein a filter is also provided in the infusion tube.

10. Apparatus according to claim 4 wherein the burette is in communication with the infusion tube via a dropper and a filter is provided in the drip chamber.

11. Apparatus according to any one of the preceding claims wherein the infusion tube is further provided with flow regulation One or more holes.

The apparatus of claim 13 wherein the pore size is about 15 μm.

15. Apparatus according to any of the preceding claims, wherein the reservoir is further connected to a stopper 16, the apparatus of claim 15, wherein the stopper puncturing device is further provided with one or more switch clamps.

17. A method of producing agarwood on a tree capable of producing agarwood, comprising delivering the agarwood inducing liquid into a tree using a device according to any of the preceding claims.

18. The method of claim 17 further comprising:

Drilling from the top to the bottom of the tree about 45 degrees and making the hole deeper than 1/2 of the diameter of the tree;

Transferring the agarwood inducing fluid into the borehole using an infusion device;

Seal the borehole.

19. The method of claim 18, wherein the agarwood inducing solution is a mixture of a 1% phytohormone solution and a 0.8% chemical solution in a ratio of 1:20 to 1:50, the chemical solution being a potassium chloride solution, the plant Hormone is the plant damage signal ethylene.

The method of any one of claims 17 to 19, wherein said tree capable of producing agarwood comprises all tree species of the genus Aquilaria.