WO2013094819A1 - Blood separating device - Google Patents

Blood separating device Download PDF

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Publication number
WO2013094819A1
WO2013094819A1 PCT/KR2012/001235 KR2012001235W WO2013094819A1 WO 2013094819 A1 WO2013094819 A1 WO 2013094819A1 KR 2012001235 W KR2012001235 W KR 2012001235W WO 2013094819 A1 WO2013094819 A1 WO 2013094819A1
Authority
WO
WIPO (PCT)
Prior art keywords
kit
blood
space
cap
opening
Prior art date
Application number
PCT/KR2012/001235
Other languages
French (fr)
Korean (ko)
Inventor
황석현
신봉근
Original Assignee
(주) 레보메드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by (주) 레보메드 filed Critical (주) 레보메드
Priority to CN201280063492.8A priority Critical patent/CN104010672B/en
Publication of WO2013094819A1 publication Critical patent/WO2013094819A1/en

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Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61MDEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/56Labware specially adapted for transferring fluids
    • B01L3/563Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
    • B01L3/5635Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors connecting two containers face to face, e.g. comprising a filter
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B7/00Elements of centrifuges
    • B04B7/08Rotary bowls
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/10Apparatus for enzymology or microbiology rotatably mounted
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/24Apparatus for enzymology or microbiology tube or bottle type
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/04Closures and closing means
    • B01L2300/041Connecting closures to device or container
    • B01L2300/042Caps; Plugs
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0681Filter
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0848Specific forms of parts of containers
    • B01L2300/0851Bottom walls
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/06Valves, specific forms thereof
    • B01L2400/0633Valves, specific forms thereof with moving parts
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021Test tubes specially adapted for centrifugation purposes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/49Blood
    • G01N33/491Blood by separating the blood components

Definitions

  • the present invention relates to a blood separation device, and more particularly to a blood separation device for extracting PRP by centrifugation.
  • Blood is composed of plasma, platelets, and blood cells, among which platelets contain the components necessary for the regeneration and activation of human tissue.
  • the platelets thus separated are plasma-rich plasma, namely, platelet rich plasma (PRP) and platelets that are not rich in plasma, That is, it consists of Platelet Poor Plasma (PPP).
  • PRP platelet rich plasma
  • PPP Platelet Poor Plasma
  • Platelets contain several growth factors and play an important role in wound healing and skin regeneration. It is reported that such platelets are contained in high concentrations in PRP, and various growth factors stimulate the proliferation of surrounding cells and stimulate abundant synthesis of collagen and other components. Accordingly, PRP has been used in various fields such as pain treatment such as low back pain, hair loss treatment, skin diseases such as skin regeneration or burn treatment.
  • the collected blood is centrifuged to separate blood cell components and platelet poor plasma (PPP, Platelet Poor Plasma) and centrifuged again to extract only PRP.
  • PPP platelet poor plasma
  • the conventional separation method using the blood separation device has a problem that the blood may be exposed to the atmosphere during the transfer to the centrifuge vessel, the transfer to the separation vessel including the filter after the blood collection.
  • the present invention has been made to solve the above-mentioned problems of the prior art, and an object of the present invention is to provide a blood separation device capable of two centrifugal separations without exposing the blood to the atmosphere. In addition, an object of the present invention is to provide a blood separation device that can be easily separated by concentrating more PRP. It is also an object of the present invention to provide a blood separation device capable of extracting exactly the desired amount with one kit.
  • one embodiment of the present invention includes a first space for receiving blood therein, the first space is a first kit, which is separated vertically through the first opening, the blood inside And a second space accommodating the second space, wherein the second space is disposed between the second kit, the first kit, and the second kit, which are vertically separated through the second opening, and the first space and the first kit.
  • a stopper having a second bar, a first cap covering an upper end of the first kit, and a second cap covering a lower end of the second kit, wherein the stopper includes the first opening and the second opening.
  • a blood separation device characterized in that a plurality of blood separations are possible by opening and closing each part.
  • the first space and the second space may be maintained in a vacuum state prior to the injection of blood.
  • the stopper is rotatably screwed with the first kit and the second kit can be moved up and down.
  • the first kit or the second kit may be provided with an opening for injecting or extracting blood.
  • the opening may be provided with a filter for preventing contamination.
  • the first cap is rotatably screwed with the first kit can be moved up and down.
  • the second cap is screwed rotatably with the second kit can be moved up and down.
  • the first cap or the second cap may comprise a member made of silicone or rubber.
  • At least one of the first kit, the second kit, the stopper, the first cap, and the second cap may be made of a transparent material.
  • a scale line may be displayed on the first cap or the second cap.
  • the first kit or the second kit may be in the form of an hourglass separated into an upper space and a lower space.
  • a sealing ring of silicon or rubber material may be disposed between the stopper and the first kit and between the stopper and the second kit.
  • the present invention it is possible to provide a blood separation device capable of two centrifugal separations without exposing the blood to the atmosphere.
  • a blood separation device that can be easily separated by concentrating more PRP.
  • the present invention can provide a blood separation device that can accurately extract the desired amount with one kit.
  • FIG. 1 is a perspective view of a blood separation apparatus according to an embodiment of the present invention.
  • FIG. 2 is an exploded perspective view of the blood separation device of FIG. 1.
  • FIG. 2 is an exploded perspective view of the blood separation device of FIG. 1.
  • FIG. 3 and 4 are cross-sectional views of the blood separation device of FIG.
  • FIG. 5 is a partial cross-sectional view schematically showing the operation of the stopper of the blood separation device of FIG.
  • FIG. 6 is a partial perspective view showing the installation form of the filter for preventing contamination.
  • FIG 7 and 8 are photographs showing the actual appearance of the blood separation apparatus according to an embodiment of the present invention.
  • FIG. 1 is a perspective view of a blood separation device according to an embodiment of the present invention
  • Figure 2 is an exploded perspective view of the blood separation device of Figure 1
  • Figure 3 and Figure 4 is a cross-sectional view of the blood separation device of Figure 1
  • Figure 5 is Figure 1
  • Partial cross-sectional view schematically showing the operation of the stopper of the blood separation device of Figure 6 is a partial perspective view showing the installation form of the filter for preventing contamination.
  • the blood separation device 1 may include a first cap 10, a first kit 20, a stopper 30, a second kit 40, and a second cap 50. Include. These components form a total of three chambers (spaces) used for the separation of blood, which are open or closed with each other.
  • the first cap 10 is provided to cover the upper end of the first kit 20.
  • the first cap 10 is the outer side 11 in contact with the outer diameter of the upper cylindrical portion 21 of the first kit 20 to be described later, and the outer side 11 is fixedly coupled to the upper cylinder of the first kit 20
  • An inner portion 12 in contact with the inner diameter of the portion 21 is included.
  • a screw portion 13 is provided at an inner diameter of the outer portion 11 of the first cap 10, and the screw portion 13 is provided with a screw portion 26 provided at an upper cylindrical portion 21 of the first kit 20, which will be described later. Screwed rotatably. By rotating the first cap 10 with respect to the first kit 20, the first cap 10 is movable up and down with respect to the first kit 20. Thereby, the amount of blood accommodated in the inner space (first space) of the first kit 20 can be adjusted.
  • the inner side 12 of the first cap 10 is fixedly coupled to the outer side 11.
  • the protrusion provided on the side inner circumferential surface of the outer portion 11 is fitted to the groove provided on the side outer circumferential surface of the inner portion 13, so that the inner portion 12 and the outer portion 11 may be fixedly coupled. .
  • the inner portion 12 may be a member having a flexible material, such as silicone or rubber.
  • a flexible material such as silicone or rubber.
  • the first kit 20 is disposed below the first cap 10 and includes a first space for receiving blood therein.
  • the first kit 20 has an inner side between the upper cylindrical portion 21, the lower cylindrical portion 22, the upper cylindrical portion 21, and the lower cylindrical portion 22 integrally connected to the upper cylindrical portion 21.
  • Inner conical portion 23 is formed to protrude into, and includes a neck portion 24 formed on the upper side of the inner conical portion (23).
  • the neck part 24 is provided with the 1st opening part 24a, and a 1st space is divided up and down into the upper space 20a and the lower space 20b by this 1st opening part 24a.
  • An upper threaded portion 26 coupled to the threaded portion 13 of the first cap 10 is provided at an upper end of the outer surface of the upper cylindrical portion 21.
  • the lower thread part 27 which engages with the upper thread part 35 of the stopper 30 mentioned later is provided in the lower end part of the outer side surface of the lower cylindrical part 22. Screw coupling between the lower cylindrical portion 22 and the stopper 30 will be described later.
  • the inner conical part 23 is formed in the shape of a cone whose diameter decreases upward toward the inner space (first space) at the portion where the upper cylindrical portion 21 and the lower cylindrical portion 22 contact each other.
  • the upper end of the inner cone 23 is provided with a tubular neck 24 formed to extend in the upward direction.
  • the first space is separated up and down through the neck 24, and the components of the blood are freely moved up and down during centrifugation of the blood.
  • the neck 24 is closed to spatially separate the blood.
  • the side portion of the inner cone 23 is provided with an opening 25 for injecting or extracting blood.
  • a cap (not shown) can be provided in close contact with the opening 25.
  • the cap may be made of silicone or rubber material, thereby allowing the blood in the syringe to be injected into the blood separation apparatus 1 or the blood having been separated can be extracted outside through the syringe.
  • the opening 25 may be provided with a filter for preventing contamination.
  • the pollution prevention filter 251 is installed in close contact with the opening 25, and the sealing ring 252 is fixedly coupled to the filter 251.
  • an opening cap 253 is provided on the sealing ring 252.
  • the stopper 30 includes an outer cylindrical portion 31, a plate 32 located at the inner diameter center portion of the outer cylindrical portion 31, a first bar 33 protruding upward from an upper surface of the central portion of the plate 32, And a second bar 34 protruding downward from the lower surface of the central portion of the plate 32.
  • the upper threaded portion 35 is engaged with the lower threaded portion 27 of the first kit 20 described above.
  • the first kit 20 can be moved up and down with respect to the stopper 30, thereby allowing the first kit 20 to be moved.
  • the first opening portion 24a can be opened and closed.
  • the second kit 40 can be moved up and down with respect to the stopper 30, thereby allowing the second kit 40 to be moved.
  • the second opening 43a can be opened and closed.
  • the first opening 24a of the first kit 20 is opened by moving the first kit 20 upward with respect to the stopper 30, and the second opening with respect to the stopper 30.
  • the kit 40 downward By moving the kit 40 downward, the state in which the second opening 43a of the second kit 40 is opened can be confirmed.
  • the sealing ring 60 may be disposed between the stopper 30 and the first kit 20.
  • the sealing ring 60 is a member made of silicone or rubber, thereby sealing the fluid so that fluid does not leak between the stopper 30 and the first kit 20.
  • sealing ring 70 may be disposed between the stopper 30 and the second kit 40.
  • the sealing ring 70 is a member made of silicon or rubber, thereby sealing the fluid so that fluid does not leak between the stopper 30 and the second kit 40.
  • the plate 32 is in the form of a thin plate, and the plate 32 is provided with a communication hole 32a penetrated up and down.
  • the communication hole 32a may have a spherical shape, a fan shape, or a half moon shape when viewed from the top, but is not limited to this shape.
  • the inner space (first space) of the first kit 20 described above and the inner space (second space) of the second kit 40 described later are in fluid communication with the communication hole 32a. That is, the lower space 20b of the first kit 20 functions substantially integrally with the upper space 40a of the second kit 40 to be described later.
  • the first bar 33 and the second bar 34 are members of a long cylindrical shape having a constant diameter, but are not limited to such a shape. End portions of the first bar 33 and the second bar 34 are in contact with the first opening 24a and the second opening 43a to prevent blood flow, and the first bar 33 and the second bar 34 may prevent the flow of blood. End portions of the bar 33 and the second bar 34 may be formed in a hemispherical shape.
  • the second kit 40 is disposed below the stopper 30 and includes a second space for receiving blood therein.
  • the second kit 20 is integrally connected to the upper cylindrical portion 41, the upper cylindrical portion 41, and the upper connecting portion 42, the diameter of which becomes smaller toward the lower side, integral with the lower end of the upper connecting portion 42.
  • Tube-shaped neck portion 43 connected to the lower, the lower connection portion 44, which is integrally connected to the neck portion 43 and the diameter increases gradually toward the lower side, the lower cylindrical portion 45 integrally connected to the lower connection portion 44 ).
  • a second opening 43a is provided in the neck 43, and the second opening 43a separates the second space into the upper space 40a and the lower space 40b up and down. That is, the second kit 40 has an hourglass shape that is divided into an upper space 40a and a lower space 40b.
  • An upper threaded portion 47 coupled to the threaded portion 36 of the stopper 30 is provided at the upper end of the outer surface of the upper cylindrical portion 41.
  • a lower threaded portion 48 is provided at the lower end of the outer surface of the lower cylindrical portion 45 to engage with the threaded portion 52 of the second cap 50 described later. Screw coupling between the lower cylindrical portion 45 and the second cap 50 will be described later.
  • the neck 43 of the second kit 40 While the second space is separated up and down through the neck 43 of the second kit 40, the components of the blood are freely moved up and down during centrifugal separation of the blood. In addition, when the centrifugation of blood is completed, the neck 43 is closed to spatially separate the blood.
  • the side portion of the upper connection portion 42 is provided with an opening 46 for injecting or extracting blood.
  • a cap (not shown) can be provided in close contact with the opening 46.
  • the cap may be made of silicone or rubber material, thereby allowing the blood in the syringe to be injected into the blood separation apparatus 1 or the blood having been separated can be extracted outside through the syringe.
  • the second cap 50 is positioned below the second kit 40 and is provided to cover the lower end of the second kit 40.
  • the second cap 50 includes an outer portion 51 in contact with the outer diameter of the lower cylindrical portion 45 of the second kit 40 described above.
  • a screw portion 52 is provided at an inner diameter of the outer portion 51 of the second cap 50, and the screw portion 52 is provided with a screw portion 48 provided in the lower cylindrical portion 45 of the second kit 40 described above. Screwed rotatably.
  • the second cap 50 By rotating the second cap 50 with respect to the second kit 40, the second cap 50 is movable up and down with respect to the second kit 40.
  • the amount of blood accommodated in the inner space of the second kit 40 can be adjusted. For example, in FIG. 3, since the second cap 50 is moved downward with respect to the second kit 40, the volume of the lower space 40b is increased, and in FIG. 4, the second cap 50 is second. Since the state moves upward with respect to the kit 40, the volume of the lower space 40b becomes small.
  • the sealing ring 80 may be disposed between the second cap 50 and the second kit 40.
  • the sealing ring 80 is a member made of silicone or rubber, thereby sealing the fluid so that fluid does not leak between the second cap 50 and the second kit 40.
  • the blood separation device 1 includes three space parts, that is, the upper space 20a of the first kit 20, the lower space 40b of the second kit 40, and the first kit which is an integral space. It is divided into the lower space 20b of 20 and the upper space 40a of the second kit 40.
  • the upper space 20a and the lower space 20b of the first kit 20 are separated by the first opening 24a
  • the upper space 40a and the lower space 40b of the second kit 40 are separated. Is separated by the second opening 43a.
  • the stopper 30 opens and closes the first opening portion 24a and the second opening portion 43a, respectively, so that a plurality of blood separations can be performed. This will be described later.
  • the first space inside the first kit 20 and the second space inside the second kit 40 may be maintained in a vacuum state before the blood is injected.
  • the phenomenon that injection becomes difficult due to internal air pressure when supplying blood in the blood separation device 1 is prevented.
  • the vacuum in the blood separation apparatus 1 may be gradually destroyed.
  • each component of the blood separation device 1 that is, the first cap 10, the first kit 20, the stopper 30, the second kit 40, and the second cap 50.
  • Silver may be formed of a transparent material. As a result, the user can easily observe the amount or separation of blood.
  • grid lines may be displayed on the first cap 10 or the second cap 50, whereby the upper space 20a of the first kit 20 and the lower space 40b of the second kit 40 may be displayed. It is possible to accurately measure or adjust the volume of.
  • the openings 25 and 46 for injecting and extracting blood are provided in both the first kit 20 and the second kit 40, but any one of these openings 25 and 46 is provided. Only one may be provided. In addition, two or more openings may be provided in only one of the first kit 20 or the second kit 40.
  • the shapes of the first kit 20 and the second kit 40 are different from each other, but the shapes of the first kit 20 and the second kit 40 may be the same.
  • the shapes may be opposite to each other.
  • the first kit 20 may be formed in an hourglass shape.
  • the shapes of the first cap 10 and the second cap 50 are different from each other, but the shapes of the first cap 10 and the second cap 50 may be the same.
  • the shapes may be opposite to each other.
  • a member made of silicon or rubber may be provided in the second cap 50.
  • the upper threaded portion 35 and the lower threaded portion 36 are respectively provided on the inner surface of the outer cylindrical portion 31, but the upper threaded portion 35 and the lower side of the outer cylindrical portion 32 are described. It is also possible for either or both of the threads 36 to be provided on the outer side of the outer cylindrical portion 32.
  • the position of the lower thread part 27 of the 1st kit 20 and the upper thread part 47 of the 2nd kit 40 is also the upper thread part 35 and the lower thread part 36 of the outer cylindrical part 31, and It should be changed to be a joinable position.
  • FIG. 7 and 8 are photographs showing the actual appearance of the blood separation device 1 according to an embodiment of the present invention.
  • the centrifugal separation of blood is made through FIG. 7, and the blood is injected into the blood separation device 1 through FIG. 8.
  • the collected blood is injected into the blood separation device 1 through the opening 25 of the first kit 20 or the opening 46 of the second kit 40.
  • the first opening 24a of the first kit 20 is closed by the first bar 33 of the stopper 30, and the second opening 43a of the second kit 40 is open.
  • the centrifugation is performed first at.
  • the plasma and buffy coat in the blood are located in the upper space 40a of the second kit 40, and the blood cells are located in the lower space 40b.
  • the second cap 50 is rotated to appropriately adjust the volume of the lower space 40b, thereby accurately separating blood components.
  • the second opening 43a of the second kit 40 is closed by the second bar 34 of the stopper 30.
  • the blood separation device 1 is turned over, that is, the first kit 20 is positioned below the second kit 40, the first opening 24a of the first kit 20 is opened. Secondary centrifugation is performed in an open state. As a result, multiplatelet plasma (PRP) and anemia platelet plasma (PPP) are formed by dividing the layer.
  • PRP multiplatelet plasma
  • PPP anemia platelet plasma
  • the stopper 30 is rotated with respect to the first kit 20. 1 Close the first opening 24a of the kit 20. In this way, the PRP in the separated blood is extracted using a syringe through the inner portion 12 of the first cap 10.
  • the blood can be centrifuged twice in the blood separation apparatus 10 without being exposed to the outside, highly concentrated PRP can be extracted without fear of infection due to the air exposure of the blood.

Abstract

In one embodiment of the present invention, provided is a blood separating device comprising: a first kit which comprises a first space for receiving blood on the inside thereof, and wherein the first space is separated between top and bottom by means of a first opening part; a second kit which comprises a second space for receiving blood on the inside thereof, and wherein the second space is separated between top and bottom by means of a second opening part; a plate which is disposed between the first kit and the second kit, and is provided with a through-connection hole for establishing fluid through connection of the first space and the second space; a stopper having a first bar which is provided projecting upwards from the plate and opens and closes the first opening part, and a second bar which is provided projecting downwards from the plate and opens and closes the second opening part; a first cap which covers the upper end part of the first kit; and a second cap which covers the bottom end part of the second kit. The stopper allows blood to be separated a plurality of times by respectively opening and closing the first opening part and the second opening part.

Description

혈액 분리 장치Blood separation device
본 발명은 혈액 분리 장치에 관한 것으로, 더욱 상세하게는 원심 분리에 의해 PRP를 추출하는 혈액 분리 장치에 관한 것이다.The present invention relates to a blood separation device, and more particularly to a blood separation device for extracting PRP by centrifugation.
혈액은 혈장, 혈소판, 및 혈구로 구성되어 있으며, 이 중 혈소판은 인체 조직의 재생과 활성화에 필요한 성분을 포함하고 있다. 혈액을 원심 분리하게 되면, 그 무게에 따라 혈장, 혈소판, 및 혈구로 분리되는데, 이렇게 분리된 혈소판은 혈장이 풍부한 혈장, 즉 다혈소판 혈장(PRP: Platelet Rich Plasma)과 혈장이 풍부하지 않은 혈소판, 즉 빈혈소판 혈장(PPP: Platelet Poor Plasma)으로 구성된다. 혈소판은 여러 성장인자들을 함유하고 있어서 상처 치유와 피부 재생에 중요한 역할을 한다. PRP 내에는 이러한 혈소판들이 고농도로 함유되어 다양한 성장 인자들이 주변 세포들의 증식을 촉진하고 콜라겐 등의 성분들을 풍부히 합성하도록 자극해 주는 효과가 있다고 보고되고 있다. 이에 따라 최근 요통과 같은 통증치료, 탈모치료, 피부재생이나 화상치료와 같은 피부질환 등 다양한 분야에서 PRP가 사용되고 있다.Blood is composed of plasma, platelets, and blood cells, among which platelets contain the components necessary for the regeneration and activation of human tissue. When centrifuged, the blood is separated into plasma, platelets, and blood cells according to their weight. The platelets thus separated are plasma-rich plasma, namely, platelet rich plasma (PRP) and platelets that are not rich in plasma, That is, it consists of Platelet Poor Plasma (PPP). Platelets contain several growth factors and play an important role in wound healing and skin regeneration. It is reported that such platelets are contained in high concentrations in PRP, and various growth factors stimulate the proliferation of surrounding cells and stimulate abundant synthesis of collagen and other components. Accordingly, PRP has been used in various fields such as pain treatment such as low back pain, hair loss treatment, skin diseases such as skin regeneration or burn treatment.
이러한 PRP를 분리하기 위한 방법으로 채혈된 혈액을 원심 분리하여 혈구성분과 빈혈소판 혈장(PPP, Platelet Poor Plasma)을 분리하고 이를 다시 원심 분리하여 PRP만을 적출하는 방법이 있다. 그러나 종래의 혈액 분리 장치를 이용한 분리 방법은 채혈 후 원심 분리용기로의 이동과정, 필터를 포함한 분리용기로의 이동과정에서 혈액이 대기에 노출될 수 있다는 문제점이 있었다. 그리고 종래의 혈액 분리 장치를 이용해서는 고농도로 혈소판을 농축시키기 어려웠으며, 또한 정확히 원하는 양만큼 PRP를 추출하는 것도 곤란하였다.As a method for separating such PRP, the collected blood is centrifuged to separate blood cell components and platelet poor plasma (PPP, Platelet Poor Plasma) and centrifuged again to extract only PRP. However, the conventional separation method using the blood separation device has a problem that the blood may be exposed to the atmosphere during the transfer to the centrifuge vessel, the transfer to the separation vessel including the filter after the blood collection. In addition, it was difficult to concentrate the platelets at a high concentration using a conventional blood separation device, and it was also difficult to extract PRP by exactly the desired amount.
선행 특허문헌은, 대한민국 공개특허공보 제10-2011-0045980호가 있다.As a prior patent document, there is Korea Patent Publication No. 10-2011-0045980.
본 발명은 전술한 종래기술의 문제점을 해결하기 위한 것으로, 혈액을 대기에 노출시키지 않은 채 2회의 원심 분리가 가능한 혈액 분리 장치를 제공하는 것을 목적으로 한다. 또한, 본 발명은 PRP를 보다 많이 농축하여 손쉽게 분리할 수 있는 혈액 분리 장치를 제공하는 것을 목적으로 한다. 또한, 본 발명은 하나의 키트로 정확하게 원하는 양을 추출할 수 있는 혈액 분리 장치를 제공하는 것을 목적으로 한다.The present invention has been made to solve the above-mentioned problems of the prior art, and an object of the present invention is to provide a blood separation device capable of two centrifugal separations without exposing the blood to the atmosphere. In addition, an object of the present invention is to provide a blood separation device that can be easily separated by concentrating more PRP. It is also an object of the present invention to provide a blood separation device capable of extracting exactly the desired amount with one kit.
상기와 같은 목적을 달성하기 위해, 본 발명의 일실시예는 내측에 혈액을 수용하는 제1 공간을 포함하며, 상기 제1 공간은 제1 개구부를 통해 상하 분리되어 있는 제1 키트, 내측에 혈액을 수용하는 제2 공간을 포함하며, 상기 제2 공간은 제2 개구부를 통해 상하 분리되어 있는 제2 키트, 상기 제1 키트 및 상기 제2 키트의 사이에 배치되며, 상기 제1 공간과 상기 제2 공간을 유체 연통시키는 연통홀이 구비된 플레이트, 상기 플레이트로부터 상측으로 돌출 설치되어 상기 제1 개구부를 개폐하는 제1 바(bar), 및 상기 플레이트로부터 하측으로 돌출 설치되어 상기 제2 개구부를 개폐하는 제2 바를 가지는 스토퍼, 상기 제1 키트의 상단부를 커버하는 제1 캡, 및 상기 제2 키트의 하단부를 커버하는 제2 캡을 포함하며, 상기 스토퍼는 상기 제1 개구부와 상기 제2 개구부를 각각 개폐함으로써 복수 회의 혈액 분리가 가능한 것을 특징으로 하는 혈액 분리 장치를 제공한다.In order to achieve the above object, one embodiment of the present invention includes a first space for receiving blood therein, the first space is a first kit, which is separated vertically through the first opening, the blood inside And a second space accommodating the second space, wherein the second space is disposed between the second kit, the first kit, and the second kit, which are vertically separated through the second opening, and the first space and the first kit. 2 a plate having a communication hole for fluid communication with the space, a first bar protruding upward from the plate to open and close the first opening, and protruding downward from the plate to open and close the second opening. A stopper having a second bar, a first cap covering an upper end of the first kit, and a second cap covering a lower end of the second kit, wherein the stopper includes the first opening and the second opening. Provided is a blood separation device, characterized in that a plurality of blood separations are possible by opening and closing each part.
본 발명의 일실시예에 있어서, 상기 제1 공간 및 상기 제2 공간은 혈액의 주입 이전에 진공 상태로 유지될 수 있다.In one embodiment of the present invention, the first space and the second space may be maintained in a vacuum state prior to the injection of blood.
본 발명의 일실시예에 있어서, 상기 스토퍼는 상기 제1 키트 및 상기 제2 키트와 회전 가능하게 나사 결합되어 상하 이동될 수 있다.In one embodiment of the present invention, the stopper is rotatably screwed with the first kit and the second kit can be moved up and down.
본 발명의 일실시예에 있어서, 상기 제1 키트 또는 상기 제2 키트에는 혈액을 주입 또는 추출하기 위한 개구부가 마련될 수 있다.In one embodiment of the present invention, the first kit or the second kit may be provided with an opening for injecting or extracting blood.
본 발명의 일실시예에 있어서, 상기 개구부에는 오염방지용 필터가 마련될 수 있다.In one embodiment of the present invention, the opening may be provided with a filter for preventing contamination.
본 발명의 일실시예에 있어서, 상기 제1 캡은 상기 제1 키트와 회전 가능하게 나사 결합되어 상하 이동될 수 있다.In one embodiment of the present invention, the first cap is rotatably screwed with the first kit can be moved up and down.
본 발명의 일실시예에 있어서, 상기 제2 캡은 상기 제2 키트와 회전 가능하게 나사 결합되어 상하 이동될 수 있다.In one embodiment of the present invention, the second cap is screwed rotatably with the second kit can be moved up and down.
본 발명의 일실시예에 있어서, 상기 제1 캡 또는 상기 제2 캡은 실리콘 또는 고무 재질의 부재를 포함할 수 있다.In one embodiment of the present invention, the first cap or the second cap may comprise a member made of silicone or rubber.
본 발명의 일실시예에 있어서, 상기 제1 키트, 상기 제2 키트, 상기 스토퍼, 상기 제1 캡, 및 상기 제2 캡 중 하나 이상은 투명한 소재로 될 수 있다.In one embodiment of the present invention, at least one of the first kit, the second kit, the stopper, the first cap, and the second cap may be made of a transparent material.
본 발명의 일실시예에 있어서, 상기 제1 캡 또는 상기 제2 캡에는 눈금선이 표시되어 있을 수 있다.In one embodiment of the present invention, a scale line may be displayed on the first cap or the second cap.
본 발명의 일실시예에 있어서, 상기 제1 키트 또는 상기 제2 키트는 상측 공간 및 하측 공간으로 분리되는 모래시계 형상으로 될 수 있다.In one embodiment of the present invention, the first kit or the second kit may be in the form of an hourglass separated into an upper space and a lower space.
본 발명의 일실시예에 있어서, 상기 스토퍼와 상기 제1 키트의 사이, 및 상기 스토퍼와 상기 제2 키트의 사이에는 실리콘 또는 고무 재질의 실링 링이 배치될 수 있다.In one embodiment of the present invention, a sealing ring of silicon or rubber material may be disposed between the stopper and the first kit and between the stopper and the second kit.
본 발명에 따르면, 혈액을 대기에 노출시키지 않은 채 2회의 원심 분리가 가능한 혈액 분리 장치를 제공할 수 있다. 또한, 본 발명에 따르면 PRP를 보다 많이 농축하여 손쉽게 분리할 수 있는 혈액 분리 장치를 제공할 수 있다. 또한, 본 발명은 하나의 키트로 정확하게 원하는 양을 추출할 수 있는 혈액 분리 장치를 제공할 수 있다.According to the present invention, it is possible to provide a blood separation device capable of two centrifugal separations without exposing the blood to the atmosphere. In addition, according to the present invention can provide a blood separation device that can be easily separated by concentrating more PRP. In addition, the present invention can provide a blood separation device that can accurately extract the desired amount with one kit.
본 발명의 효과는 상기한 효과로 한정되는 것은 아니며, 본 발명의 상세한 설명 또는 특허청구범위에 기재된 발명의 구성으로부터 추론가능한 모든 효과를 포함하는 것으로 이해되어야 한다.The effects of the present invention are not limited to the above-described effects, but should be understood to include all the effects deduced from the configuration of the invention described in the detailed description or claims of the present invention.
도 1은 본 발명의 일실시예에 의한 혈액 분리 장치의 사시도이다.1 is a perspective view of a blood separation apparatus according to an embodiment of the present invention.
도 2는 도 1의 혈액 분리 장치의 분해 사시도이다.FIG. 2 is an exploded perspective view of the blood separation device of FIG. 1. FIG.
도 3 및 도 4는 도 1의 혈액 분리 장치의 단면도이다.3 and 4 are cross-sectional views of the blood separation device of FIG.
도 5는 도 1의 혈액 분리 장치의 스토퍼의 작동을 개략적으로 나타낸 부분 단면도이다.5 is a partial cross-sectional view schematically showing the operation of the stopper of the blood separation device of FIG.
도 6은 오염방지용 필터의 설치 형태를 나타낸 부분 사시도이다.6 is a partial perspective view showing the installation form of the filter for preventing contamination.
도 7 및 도 8은 본 발명의 일실시예에 의한 혈액 분리 장치의 실제 모습을 나타낸 사진이다.7 and 8 are photographs showing the actual appearance of the blood separation apparatus according to an embodiment of the present invention.
이하에서는 첨부한 도면을 참조하여 본 발명을 설명하기로 한다. 그러나 본 발명은 여러 가지 상이한 형태로 구현될 수 있으며, 따라서 여기에서 설명하는 실시예로 한정되는 것은 아니다. 그리고 도면에서 본 발명을 명확하게 설명하기 위해서 설명과 관계없는 부분은 생략하였으며, 명세서 전체를 통하여 유사한 부분에 대해서는 유사한 도면 부호를 붙였다.Hereinafter, with reference to the accompanying drawings will be described the present invention. As those skilled in the art would realize, the described embodiments may be modified in various different ways, all without departing from the spirit or scope of the present invention. In the drawings, parts irrelevant to the description are omitted in order to clearly describe the present invention, and like reference numerals designate like parts throughout the specification.
명세서 전체에서, 어떤 부분이 다른 부분과 "연결"되어 있다고 할 때, 이는 "직접적으로 연결"되어 있는 경우뿐 아니라, 그 중간에 다른 부재를 사이에 두고 "간접적으로 연결"되어 있는 경우도 포함한다. 또한 어떤 부분이 어떤 구성요소를 "포함"한다고 할 때, 이는 특별히 반대되는 기재가 없는 한 다른 구성요소를 제외하는 것이 아니라 다른 구성요소를 더 구비할 수 있다는 것을 의미한다.Throughout the specification, when a part is "connected" to another part, it includes not only "directly connected" but also "indirectly connected" with another member in between. . In addition, when a part is said to "include" a certain component, this means that it may further include other components, without excluding the other components unless otherwise stated.
이하 첨부된 도면을 참고하여 본 발명의 실시예를 상세히 설명하기로 한다.Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.
도 1은 본 발명의 일실시예에 의한 혈액 분리 장치의 사시도, 도 2는 도 1의 혈액 분리 장치의 분해 사시도, 도 3 및 도 4는 도 1의 혈액 분리 장치의 단면도, 도 5는 도 1의 혈액 분리 장치의 스토퍼의 작동을 개략적으로 나타낸 부분 단면도, 도 6은 오염방지용 필터의 설치 형태를 나타낸 부분 사시도이다.1 is a perspective view of a blood separation device according to an embodiment of the present invention, Figure 2 is an exploded perspective view of the blood separation device of Figure 1, Figure 3 and Figure 4 is a cross-sectional view of the blood separation device of Figure 1, Figure 5 is Figure 1 Partial cross-sectional view schematically showing the operation of the stopper of the blood separation device of Figure 6 is a partial perspective view showing the installation form of the filter for preventing contamination.
도 1 및 도 2를 참조하면, 혈액 분리 장치(1)는 제1 캡(10), 제1 키트(20), 스토퍼(30), 제2 키트(40), 및 제2 캡(50)을 포함한다. 이들 구성요소들에 의하여 혈액의 분리에 이용되는 총 3개의 챔버(공간부)가 형성되며, 이들 챔버는 각각 서로 개방 또는 폐쇄된다.1 and 2, the blood separation device 1 may include a first cap 10, a first kit 20, a stopper 30, a second kit 40, and a second cap 50. Include. These components form a total of three chambers (spaces) used for the separation of blood, which are open or closed with each other.
도 3 및 도 4를 참조하여 각 구성요소들을 상세히 살펴보기로 한다. 먼저, 제1 캡(10)은 제1 키트(20)의 상단부를 커버하도록 마련된다. 제1 캡(10)은 후술하는 제1 키트(20)의 상측 원통부(21)의 외경에 접하는 외측부(11), 및 이 외측부(11)에 고정 결합되며 제1 키트(20)의 상측 원통부(21)의 내경에 접하는 내측부(12)를 포함한다.Each component will be described in detail with reference to FIGS. 3 and 4. First, the first cap 10 is provided to cover the upper end of the first kit 20. The first cap 10 is the outer side 11 in contact with the outer diameter of the upper cylindrical portion 21 of the first kit 20 to be described later, and the outer side 11 is fixedly coupled to the upper cylinder of the first kit 20 An inner portion 12 in contact with the inner diameter of the portion 21 is included.
제1 캡(10)의 외측부(11)의 내경에는 나사부(13)가 마련되며, 이 나사부(13)는 후술하는 제1 키트(20)의 상측 원통부(21)에 마련된 나사부(26)와 회전 가능하게 나사 결합된다. 제1 키트(20)에 대하여 제1 캡(10)을 회전함으로써, 제1 캡(10)은 제1 키트(20)에 대해 상하 이동 가능하게 된다. 이에 의해 제1 키트(20)의 내측 공간(제1 공간)에 수용되는 혈액의 양이 조절 가능하게 된다.A screw portion 13 is provided at an inner diameter of the outer portion 11 of the first cap 10, and the screw portion 13 is provided with a screw portion 26 provided at an upper cylindrical portion 21 of the first kit 20, which will be described later. Screwed rotatably. By rotating the first cap 10 with respect to the first kit 20, the first cap 10 is movable up and down with respect to the first kit 20. Thereby, the amount of blood accommodated in the inner space (first space) of the first kit 20 can be adjusted.
제1 캡(10)의 내측부(12)는 외측부(11)에 고정 결합된다. 예컨대 도 3에 도시된 바와 같이, 내측부(13)의 측면 외주면에 마련된 홈에 외측부(11)의 측면 내주면에 마련된 돌출부가 끼워맞춤되어, 내측부(12)와 외측부(11)가 고정 결합될 수 있다.The inner side 12 of the first cap 10 is fixedly coupled to the outer side 11. For example, as shown in FIG. 3, the protrusion provided on the side inner circumferential surface of the outer portion 11 is fitted to the groove provided on the side outer circumferential surface of the inner portion 13, so that the inner portion 12 and the outer portion 11 may be fixedly coupled. .
내측부(12)는 가요성을 갖는 재질, 예컨대 실리콘 또는 고무 재질의 부재일 수 있다. 내측부(12)를 통해 주사 바늘을 삽입함으로써, 혈액 분리 장치(1)의 내부에 혈액을 주입할 수도 있고, 혈액 분리 장치(1)로부터 분리가 완료된 혈액을 추출할 수도 있다. 내측부(12)는 가요성을 갖는 재질로 형성되기 때문에 내측부(12)는 제1 키트(20)의 상측 원통부(21)에 밀착되도록 결합될 수 있다. 이로써 제1 캡(10)과 제1 키트(20) 사이에서 별도의 실링 부재 없이도 유체가 누설되지 않도록 밀봉이 이루어진다.The inner portion 12 may be a member having a flexible material, such as silicone or rubber. By inserting the injection needle through the inner portion 12, blood may be injected into the blood separation device 1, or blood separated from the blood separation device 1 may be extracted. Since the inner part 12 is formed of a flexible material, the inner part 12 may be coupled to be in close contact with the upper cylindrical part 21 of the first kit 20. As a result, sealing is performed between the first cap 10 and the first kit 20 so that the fluid does not leak without a separate sealing member.
제1 키트(20)는 제1 캡(10)의 하측에 배치되며, 그 내측에 혈액을 수용하는 제1 공간을 포함한다. 제1 키트(20)는 상측 원통부(21), 이 상측 원통부(21)에 일체로 연결되는 하측 원통부(22), 상측 원통부(21) 및 하측 원통부(22)의 사이에서 내측으로 돌출 형성되는 내측 원뿔부(23), 내측 원뿔부(23)의 상측에 형성되는 목부(24)를 포함한다. 목부(24)에는 제1 개구부(24a)가 마련되며, 이 제1 개구부(24a)에 의해 제1 공간이 상측 공간(20a)과 하측 공간(20b)으로 상하 분리된다.The first kit 20 is disposed below the first cap 10 and includes a first space for receiving blood therein. The first kit 20 has an inner side between the upper cylindrical portion 21, the lower cylindrical portion 22, the upper cylindrical portion 21, and the lower cylindrical portion 22 integrally connected to the upper cylindrical portion 21. Inner conical portion 23 is formed to protrude into, and includes a neck portion 24 formed on the upper side of the inner conical portion (23). The neck part 24 is provided with the 1st opening part 24a, and a 1st space is divided up and down into the upper space 20a and the lower space 20b by this 1st opening part 24a.
상측 원통부(21)의 외측면 상단부에는 전술한 제1 캡(10)의 나사부(13)와 결합되는 상측 나사부(26)가 마련된다. 하측 원통부(22)의 외측면 하단부에는 후술하는 스토퍼(30)의 상측 나사부(35)와 결합하는 하측 나사부(27)가 마련된다. 하측 원통부(22)와 스토퍼(30) 사이의 나사 결합에 대해서는 후술하기로 한다.An upper threaded portion 26 coupled to the threaded portion 13 of the first cap 10 is provided at an upper end of the outer surface of the upper cylindrical portion 21. The lower thread part 27 which engages with the upper thread part 35 of the stopper 30 mentioned later is provided in the lower end part of the outer side surface of the lower cylindrical part 22. Screw coupling between the lower cylindrical portion 22 and the stopper 30 will be described later.
내측 원뿔부(23)는 상측 원통부(21)와 하측 원통부(22)가 접하는 부분에서 내측 공간(제1 공간)을 향하여 상측으로 직경이 작아지는 원뿔 모양으로 형성된다. 내측 원뿔부(23)의 상단에는 상측 방향으로 연장되도록 형성된 튜브 형상의 목부(24)가 마련된다. 이 목부(24)를 통해 제1 공간이 상하측으로 분리됨과 동시에, 혈액의 원심 분리시 혈액의 구성 성분들이 자유롭게 상하로 이동하게 된다. 또한, 혈액의 원심 분리가 완료될 경우 이 목부(24)를 폐쇄함으로써 혈액을 공간적으로 분리하게 된다.The inner conical part 23 is formed in the shape of a cone whose diameter decreases upward toward the inner space (first space) at the portion where the upper cylindrical portion 21 and the lower cylindrical portion 22 contact each other. The upper end of the inner cone 23 is provided with a tubular neck 24 formed to extend in the upward direction. The first space is separated up and down through the neck 24, and the components of the blood are freely moved up and down during centrifugation of the blood. In addition, when the centrifugation of blood is completed, the neck 24 is closed to spatially separate the blood.
내측 원뿔부(23)의 측면부에는 혈액을 주입하거나 추출하기 위한 개구부(25)가 마련된다. 이 개구부(25)에는 도시하지 않은 캡이 밀착 설치될 수 있다. 이 캡은 실리콘 또는 고무 재질로 이루어질 수 있으며, 이로써 주사기 내의 혈액을 혈액 분리 장치(1)의 내로 주입하거나, 또는 분리가 완료된 혈액을 주사기를 통해 외부로 추출할 수 있게 된다.The side portion of the inner cone 23 is provided with an opening 25 for injecting or extracting blood. A cap (not shown) can be provided in close contact with the opening 25. The cap may be made of silicone or rubber material, thereby allowing the blood in the syringe to be injected into the blood separation apparatus 1 or the blood having been separated can be extracted outside through the syringe.
한편, 개구부(25)에는 오염방지용 필터가 마련될 수 있다. 도 6을 참조하면, 개구부(25)에 오염방지용 필터(251)가 밀착 설치되고, 필터(251) 상에 실링 링(252)이 고정 결합된다. 또한 실링 링(252) 상에 개구부 캡(253)이 마련된다. 이로써, 혈액 분리 장치(1) 내의 진공이 파괴된 경우, 개구부 캡(253)을 열고 필터(251)를 통해 혈액 분리 장치(1) 내에 공기 연통이 가능하게 된 상태에서, 후술하는 다른 개구부(46)를 통해 혈액을 혈액 분리 장치(1)의 내로 주입하거나, 분리가 완료된 혈액을 외부로 추출할 수 있게 된다.스토퍼(30)는 제1 키트(20)와 제2 키트(40)의 사이에 배치된다. 스토퍼(30)는 외측 원통부(31), 외측 원통부(31)의 내경 중앙부에 위치한 플레이트(32), 플레이트(32)의 중앙부 상면으로부터 상측으로 돌출 설치된 제1 바(bar)(33), 플레이트(32)의 중앙부 하면으로부터 하측으로 돌출 설치된 제2 바(34)를 포함한다.On the other hand, the opening 25 may be provided with a filter for preventing contamination. Referring to FIG. 6, the pollution prevention filter 251 is installed in close contact with the opening 25, and the sealing ring 252 is fixedly coupled to the filter 251. In addition, an opening cap 253 is provided on the sealing ring 252. Thus, when the vacuum in the blood separation device 1 is broken, the other openings 46 described later are opened in the state in which the opening cap 253 is opened and air communication in the blood separation device 1 is enabled through the filter 251. Blood may be injected into the blood separation device 1 or extracted with blood separated therefrom. The stopper 30 may be disposed between the first kit 20 and the second kit 40. Is placed. The stopper 30 includes an outer cylindrical portion 31, a plate 32 located at the inner diameter center portion of the outer cylindrical portion 31, a first bar 33 protruding upward from an upper surface of the central portion of the plate 32, And a second bar 34 protruding downward from the lower surface of the central portion of the plate 32.
외측 원통부(31)의 내측면 상측에는 전술한 제1 키트(20)의 하측 나사부(27)와 결합하는 상측 나사부(35)가 마련된다. 이와 같이 스토퍼(30)와 제1 키트(20)가 회전 가능하게 나사 결합됨으로써, 스토퍼(30)에 대해 제1 키트(20)를 상하 이동시킬 수 있게 되고, 이에 의해 제1 키트(20)의 제1 개구부(24a)를 개폐할 수 있게 된다.On the inner side of the outer cylindrical portion 31, the upper threaded portion 35 is engaged with the lower threaded portion 27 of the first kit 20 described above. As the stopper 30 and the first kit 20 are rotatably screwed as described above, the first kit 20 can be moved up and down with respect to the stopper 30, thereby allowing the first kit 20 to be moved. The first opening portion 24a can be opened and closed.
외측 원통부(31)의 내측면 하측에는 후술할 제2 키트(40)의 상측 나사부(47)와 결합하는 하측 나사부(36)가 마련된다. 이와 같이 스토퍼(30)와 제2 키트(40)가 회전 가능하게 나사 결합됨으로써, 스토퍼(30)에 대해 제2 키트(40)를 상하 이동시킬 수 있게 되고, 이에 의해 제2 키트(40)의 제2 개구부(43a)를 개폐할 수 있게 된다.Below the inner side of the outer cylindrical portion 31 is provided with a lower threaded portion 36 to engage with the upper threaded portion 47 of the second kit 40 to be described later. As the stopper 30 and the second kit 40 are rotatably screwed in this manner, the second kit 40 can be moved up and down with respect to the stopper 30, thereby allowing the second kit 40 to be moved. The second opening 43a can be opened and closed.
도 5를 참조하면, 스토퍼(30)에 대해 제1 키트(20)를 상측으로 이동시킴으로써 제1 키트(20)의 제1 개구부(24a)가 개방되어 있으며, 또한 스토퍼(30)에 대해 제2 키트(40)를 하측으로 이동시킴으로써 제2 키트(40)의 제2 개구부(43a)가 개방된 상태를 확인할 수 있다.Referring to FIG. 5, the first opening 24a of the first kit 20 is opened by moving the first kit 20 upward with respect to the stopper 30, and the second opening with respect to the stopper 30. By moving the kit 40 downward, the state in which the second opening 43a of the second kit 40 is opened can be confirmed.
다시 도 3 및 도 4를 참조하면, 스토퍼(30)와 제1 키트(20)의 사이에는 실링 링(60)이 배치될 수 있다. 이 실링 링(60)은 실리콘 또는 고무 재질의 부재로서, 이에 의해 스토퍼(30)와 제1 키트(20)의 사이로 유체가 누설되지 않도록 밀봉이 이루어진다.3 and 4, the sealing ring 60 may be disposed between the stopper 30 and the first kit 20. The sealing ring 60 is a member made of silicone or rubber, thereby sealing the fluid so that fluid does not leak between the stopper 30 and the first kit 20.
또한, 스토퍼(30)와 제2 키트(40)의 사이에도 실링 링(70)이 배치될 수 있다. 이 실링 링(70)은 실리콘 또는 고무 재질의 부재로서, 이에 의해 스토퍼(30)와 제2 키트(40)의 사이로 유체가 누설되지 않도록 밀봉이 이루어진다.In addition, the sealing ring 70 may be disposed between the stopper 30 and the second kit 40. The sealing ring 70 is a member made of silicon or rubber, thereby sealing the fluid so that fluid does not leak between the stopper 30 and the second kit 40.
플레이트(32)는 얇은 판 형태로 되어 있으며, 이 플레이트(32)에는 상하로 관통된 연통홀(32a)이 마련된다. 연통홀(32a)은 상면에서 보아 구형, 부채꼴, 또는 반달형일 수 있으나, 이러한 형상으로 한정되는 것은 아니다. 연통홀(32a)에 의하여 전술한 제1 키트(20)의 내측 공간(제1 공간)과 후술한 제2 키트(40)의 내측 공간(제2 공간)이 유체 연통된다. 즉, 제1 키트(20)의 하측 공간(20b)은 후술할 제2 키트(40)의 상측 공간(40a)과 실질적으로 일체로 작용하게 된다.The plate 32 is in the form of a thin plate, and the plate 32 is provided with a communication hole 32a penetrated up and down. The communication hole 32a may have a spherical shape, a fan shape, or a half moon shape when viewed from the top, but is not limited to this shape. The inner space (first space) of the first kit 20 described above and the inner space (second space) of the second kit 40 described later are in fluid communication with the communication hole 32a. That is, the lower space 20b of the first kit 20 functions substantially integrally with the upper space 40a of the second kit 40 to be described later.
제1 바(33) 및 제2 바(34)는 직경이 일정한 긴 원기둥 형상의 부재이나, 이러한 형상으로 한정되는 것은 아니다. 제1 바(33)와 제2 바(34)의 단부는 제1 개구부(24a)와 제2 개구부(43a)와 접하여 혈액의 흐름을 방지하게 되며, 이러할 혈액 흐름의 방지라는 기능을 위해 제1 바(33)와 제2 바(34)의 단부는 반구 형상으로 형성될 수 있다.The first bar 33 and the second bar 34 are members of a long cylindrical shape having a constant diameter, but are not limited to such a shape. End portions of the first bar 33 and the second bar 34 are in contact with the first opening 24a and the second opening 43a to prevent blood flow, and the first bar 33 and the second bar 34 may prevent the flow of blood. End portions of the bar 33 and the second bar 34 may be formed in a hemispherical shape.
제2 키트(40)는 스토퍼(30)의 하측에 배치되며, 그 내측에 혈액을 수용하는 제2 공간을 포함한다. 제2 키트(20)는 상측 원통부(41), 이 상측 원통부(41)에 일체로 연결되며 직경이 하측으로 갈수록 점점 작아지는 상측 연결부(42), 이 상측 연결부(42)의 하단부에 일체로 연결되는 튜브 형상의 목부(43), 이 목부(43)에 일체로 연결되며 직경이 하측으로 갈수록 점점 커지는 하측 연결부(44), 이 하측 연결부(44)에 일체로 연결되는 하측 원통부(45)를 포함한다. 목부(43)에는 제2 개구부(43a)가 마련되며, 이 제2 개구부(43a)에 의해 제2 공간이 상측 공간(40a)과 하측 공간(40b)으로 상하 분리된다. 즉, 제2 키트(40)는 상측 공간(40a)과 하측 공간(40b)으로 분리되는 모래시계 형상으로 이루어져 있다.The second kit 40 is disposed below the stopper 30 and includes a second space for receiving blood therein. The second kit 20 is integrally connected to the upper cylindrical portion 41, the upper cylindrical portion 41, and the upper connecting portion 42, the diameter of which becomes smaller toward the lower side, integral with the lower end of the upper connecting portion 42. Tube-shaped neck portion 43 connected to the lower, the lower connection portion 44, which is integrally connected to the neck portion 43 and the diameter increases gradually toward the lower side, the lower cylindrical portion 45 integrally connected to the lower connection portion 44 ). A second opening 43a is provided in the neck 43, and the second opening 43a separates the second space into the upper space 40a and the lower space 40b up and down. That is, the second kit 40 has an hourglass shape that is divided into an upper space 40a and a lower space 40b.
상측 원통부(41)의 외측면 상단부에는 전술한 스토퍼(30)의 나사부(36)와 결합되는 상측 나사부(47)가 마련된다. 하측 원통부(45)의 외측면 하단부에는 후술하는 제2 캡(50)의 나사부(52)와 결합하는 하측 나사부(48)가 마련된다. 하측 원통부(45)와 제2 캡(50) 사이의 나사 결합에 대해서는 후술하기로 한다.An upper threaded portion 47 coupled to the threaded portion 36 of the stopper 30 is provided at the upper end of the outer surface of the upper cylindrical portion 41. A lower threaded portion 48 is provided at the lower end of the outer surface of the lower cylindrical portion 45 to engage with the threaded portion 52 of the second cap 50 described later. Screw coupling between the lower cylindrical portion 45 and the second cap 50 will be described later.
한편, 제2 키트(40)의 목부(43)를 통해 제2 공간이 상하측으로 분리됨과 동시에, 혈액의 원심 분리시 혈액의 구성 성분들이 자유롭게 상하로 이동하게 된다. 또한, 혈액의 원심 분리가 완료될 경우 이 목부(43)를 폐쇄함으로써 혈액을 공간적으로 분리하게 된다.On the other hand, while the second space is separated up and down through the neck 43 of the second kit 40, the components of the blood are freely moved up and down during centrifugal separation of the blood. In addition, when the centrifugation of blood is completed, the neck 43 is closed to spatially separate the blood.
상측 연결부(42)의 측면부에는 혈액을 주입하거나 추출하기 위한 개구부(46)가 마련된다. 이 개구부(46)에는 도시하지 않은 캡이 밀착 설치될 수 있다. 이 캡은 실리콘 또는 고무 재질로 이루어질 수 있으며, 이로써 주사기 내의 혈액을 혈액 분리 장치(1)의 내로 주입하거나, 또는 분리가 완료된 혈액을 주사기를 통해 외부로 추출할 수 있게 된다.The side portion of the upper connection portion 42 is provided with an opening 46 for injecting or extracting blood. A cap (not shown) can be provided in close contact with the opening 46. The cap may be made of silicone or rubber material, thereby allowing the blood in the syringe to be injected into the blood separation apparatus 1 or the blood having been separated can be extracted outside through the syringe.
제2 캡(50)은 제2 키트(40)의 하측에 위치하며, 제2 키트(40)의 하단부를 커버하도록 마련된다. 제2 캡(50)은 전술한 제2 키트(40)의 하측 원통부(45)의 외경에 접하는 외측부(51)를 포함한다.The second cap 50 is positioned below the second kit 40 and is provided to cover the lower end of the second kit 40. The second cap 50 includes an outer portion 51 in contact with the outer diameter of the lower cylindrical portion 45 of the second kit 40 described above.
제2 캡(50)의 외측부(51)의 내경에는 나사부(52)가 마련되며, 이 나사부(52)는 전술한 제2 키트(40)의 하측 원통부(45)에 마련된 나사부(48)와 회전 가능하게 나사 결합된다. 제2 키트(40)에 대하여 제2 캡(50)을 회전함으로써, 제2 캡(50)은 제2 키트(40)에 대해 상하 이동 가능하게 된다. 이에 의해 제2 키트(40)의 내부 공간부에 수용되는 혈액의 양이 조절 가능하게 된다. 예컨대, 도 3에서는 제2 캡(50)이 제2 키트(40)에 대해 하측으로 이동한 상태이므로 하측 공간(40b)의 부피가 커지게 되고, 도 4에서는 제2 캡(50)이 제2 키트(40)에 대해 상측으로 이동한 상태이므로 하측 공간(40b)의 부피가 작아지게 된다.A screw portion 52 is provided at an inner diameter of the outer portion 51 of the second cap 50, and the screw portion 52 is provided with a screw portion 48 provided in the lower cylindrical portion 45 of the second kit 40 described above. Screwed rotatably. By rotating the second cap 50 with respect to the second kit 40, the second cap 50 is movable up and down with respect to the second kit 40. As a result, the amount of blood accommodated in the inner space of the second kit 40 can be adjusted. For example, in FIG. 3, since the second cap 50 is moved downward with respect to the second kit 40, the volume of the lower space 40b is increased, and in FIG. 4, the second cap 50 is second. Since the state moves upward with respect to the kit 40, the volume of the lower space 40b becomes small.
제2 캡(50)과 제2 키트(40)의 사이에는 실링 링(80)이 배치될 수 있다. 이 실링 링(80)은 실리콘 또는 고무 재질의 부재로서, 이에 의해 제2 캡(50)과 제2 키트(40)의 사이로 유체가 누설되지 않도록 밀봉이 이루어진다.The sealing ring 80 may be disposed between the second cap 50 and the second kit 40. The sealing ring 80 is a member made of silicone or rubber, thereby sealing the fluid so that fluid does not leak between the second cap 50 and the second kit 40.
이와 같이, 혈액 분리 장치(1)는 3개의 공간부, 즉 제1 키트(20)의 상측 공간(20a), 제2 키트(40)의 하측 공간(40b), 그리고 일체의 공간부인 제1 키트(20)의 하측 공간(20b)과 제2 키트(40)의 상측 공간(40a)으로 구획된다. 여기서, 제1 키트(20)의 상측 공간(20a)과 하측 공간(20b)은 제1 개구부(24a)에 의해 분리되고, 제2 키트(40)의 상측 공간(40a)과 하측 공간(40b)은 제2 개구부(43a)에 의해 분리된다. 또한, 스토퍼(30)는 제1 개구부(24a)와 제2 개구부(43a)를 각각 개폐함으로써 복수 회의 혈액 분리가 가능하게 된다. 이에 대해서는 후술하기로 한다.As described above, the blood separation device 1 includes three space parts, that is, the upper space 20a of the first kit 20, the lower space 40b of the second kit 40, and the first kit which is an integral space. It is divided into the lower space 20b of 20 and the upper space 40a of the second kit 40. Here, the upper space 20a and the lower space 20b of the first kit 20 are separated by the first opening 24a, and the upper space 40a and the lower space 40b of the second kit 40 are separated. Is separated by the second opening 43a. In addition, the stopper 30 opens and closes the first opening portion 24a and the second opening portion 43a, respectively, so that a plurality of blood separations can be performed. This will be described later.
한편, 제1 키트(20) 내부의 제1 공간과 제2 키트(40) 내부의 제2 공간은 혈액의 주입 이전에 진공 상태로 유지될 수 있다. 제1 공간 및 제2 공간이 진공 상태로 유지됨으로써, 혈액 분리 장치(1) 내에 혈액을 공급할 때에 내부 공기 압력에 의해 주입이 어려워지는 현상이 방지된다. 또한 혈액 주입시 외부 공기의 순환 통로를 만들 필요가 없어져 구조가 간단해 지고, 혈액의 주입도 간편하게 된다.Meanwhile, the first space inside the first kit 20 and the second space inside the second kit 40 may be maintained in a vacuum state before the blood is injected. By maintaining the first space and the second space in a vacuum state, the phenomenon that injection becomes difficult due to internal air pressure when supplying blood in the blood separation device 1 is prevented. In addition, when the blood is injected, there is no need to create a circulation passage of the outside air, thereby simplifying the structure and injecting the blood.
한편, 제조후 시간이 지나면 혈액 분리 장치(1) 내의 진공이 서서히 파괴될 수 있다. 이와 같이 혈액 분리 장치(1) 내의 진공이 유지되지 않는 경우에는 어느 하나의 개구부(25)를 개방한 상태에서, 다른 하나의 개구부(46)를 통해 혈액을 주입하는 것도 가능하다.On the other hand, if time passes after manufacture, the vacuum in the blood separation apparatus 1 may be gradually destroyed. Thus, when the vacuum in the blood separation apparatus 1 is not maintained, it is also possible to inject blood through the other opening 46 in the state which the one opening 25 was open.
한편, 혈액 분리 장치(1)의 각 구성요소, 즉 제1 캡(10), 제1 키트(20), 스토퍼(30), 제2 키트(40), 및 제2 캡(50) 중 하나 이상은 투명한 소재로 형성될 수 있다. 이로써, 사용자가 혈액의 양이나 분리 상태를 손쉽게 관찰할 수 있게 된다.Meanwhile, at least one of each component of the blood separation device 1, that is, the first cap 10, the first kit 20, the stopper 30, the second kit 40, and the second cap 50. Silver may be formed of a transparent material. As a result, the user can easily observe the amount or separation of blood.
한편, 제1 캡(10) 또는 제2 캡(50)에는 눈금선이 표시될 수 있으며, 이로써 제1 키트(20)의 상측 공간(20a) 및 제2 키트(40)의 하측 공간(40b)의 부피를 정확하게 측정하거나 조정하는 것이 가능하게 된다.Meanwhile, grid lines may be displayed on the first cap 10 or the second cap 50, whereby the upper space 20a of the first kit 20 and the lower space 40b of the second kit 40 may be displayed. It is possible to accurately measure or adjust the volume of.
한편, 전술한 실시예에서는 제1 키트(20) 및 제2 키트(40)에 모두 혈액의 주입 및 추출을 위한 개구부(25; 46)가 마련된 것으로 설명하였으나, 이들 개구부(25; 46) 중 어느 하나만 마련되어 있어도 된다. 또한, 제1 키트(20) 또는 제2 키트(40) 중 어느 하나에만 2개 이상의 개구부가 마련되는 것도 가능하다.Meanwhile, in the above-described embodiment, the openings 25 and 46 for injecting and extracting blood are provided in both the first kit 20 and the second kit 40, but any one of these openings 25 and 46 is provided. Only one may be provided. In addition, two or more openings may be provided in only one of the first kit 20 or the second kit 40.
또한, 전술한 실시예에서는 제1 키트(20)와 제2 키트(40)의 형상이 서로 상이한 것으로 설명하였으나, 제1 키트(20)와 제2 키트(40)의 형상이 서로 동일한 것이어도 되며, 그 형상이 서로 반대여도 된다. 예컨대, 제1 키트(20)가 모래 시계 형상으로 이루어질 수도 있다.In addition, in the above-described embodiment, the shapes of the first kit 20 and the second kit 40 are different from each other, but the shapes of the first kit 20 and the second kit 40 may be the same. The shapes may be opposite to each other. For example, the first kit 20 may be formed in an hourglass shape.
또한, 전술한 실시예에서는 제1 캡(10)과 제2 캡(50)의 형상이 서로 상이한 것으로 설명하였으나, 제1 캡(10)과 제2 캡(50)의 형상이 서로 동일한 것이어도 되며, 그 형상이 서로 반대여도 된다. 예컨대, 제2 캡(50)에 실리콘 또는 고무 재질의 부재가 마련되어도 좋다.In addition, in the above-described embodiment, the shapes of the first cap 10 and the second cap 50 are different from each other, but the shapes of the first cap 10 and the second cap 50 may be the same. The shapes may be opposite to each other. For example, a member made of silicon or rubber may be provided in the second cap 50.
또한, 전술한 실시예에서는 외측 원통부(31)의 내측면에 상측 나사부(35)와 하측 나사부(36)가 각각 설치되는 것으로 설명하였으나, 외측 원통부(32)의 상측 나사부(35)와 하측 나사부(36)의 어느 하나, 또는 둘 모두가 외측 원통부(32)의 외측면에 설치되는 것도 가능하다. 이 경우 제1 키트(20)의 하측 나사부(27)와 제2 키트(40)의 상측 나사부(47)의 위치도, 외측 원통부(31)의 상측 나사부(35)와 하측 나사부(36)와 결합가능한 위치가 되도록 변경되어야 한다.In addition, in the above-described embodiment, the upper threaded portion 35 and the lower threaded portion 36 are respectively provided on the inner surface of the outer cylindrical portion 31, but the upper threaded portion 35 and the lower side of the outer cylindrical portion 32 are described. It is also possible for either or both of the threads 36 to be provided on the outer side of the outer cylindrical portion 32. In this case, the position of the lower thread part 27 of the 1st kit 20 and the upper thread part 47 of the 2nd kit 40 is also the upper thread part 35 and the lower thread part 36 of the outer cylindrical part 31, and It should be changed to be a joinable position.
도 7 및 도 8은 본 발명의 일실시예에 의한 혈액 분리 장치(1)의 실제 모습을 나타낸 사진이다. 도 7을 통해 혈액 분리 장치(1)를 통해 혈액의 원심 분리가 이루어진 모습을 확인할 수 있으며, 도 8을 통해 혈액 분리 장치(1) 내에 혈액을 주입하는 모습을 확인할 수 있다.7 and 8 are photographs showing the actual appearance of the blood separation device 1 according to an embodiment of the present invention. Through the blood separation device 1, it can be seen that the centrifugal separation of blood is made through FIG. 7, and the blood is injected into the blood separation device 1 through FIG. 8.
다음으로, 본 발명의 일실시예에 의한 혈액 분리 장치(1)를 이용하여 2회의 혈액 원심 분리를 행하는 방법을 설명하기로 한다.Next, a method of performing two blood centrifugation using the blood separation device 1 according to one embodiment of the present invention will be described.
먼저, 채혈한 혈액을 제1 키트(20)의 개구부(25) 또는 제2 키트(40)의 개구부(46)를 통해 혈액 분리 장치(1) 내로 주입한다. 다음으로, 제1 키트(20)의 제1 개구부(24a)는 스토퍼(30)의 제1 바(33)에 의해 폐쇄되고, 제2 키트(40)의 제2 개구부(43a)는 개방된 상태에서 1차로 원심 분리를 행한다. 1차 원심 분리가 완료되면 혈액 중의 혈장 및 버피 코트는 제2 키트(40)의 상측 공간(40a)에 위치하고, 혈구는 하측 공간(40b)에 위치하게 된다. 이 때 제2 캡(50)을 회전시켜 하측 공간(40b)의 부피를 적절히 조정함으로써, 혈액 성분을 정확히 분리하게 된다. 이후, 제2 키트(40)의 제2 개구부(43a)를 스토퍼(30)의 제2 바(34)에 의해 폐쇄한다. 다음으로, 혈액 분리 장치(1)를 뒤집은 후, 즉 제1 키트(20)가 제2 키트(40)에 대해 하측에 위치하도록 한 후, 제1 키트(20)의 제1 개구부(24a)를 개방한 상태로 2차 원심 분리를 행한다. 이에 의해 다혈소판 혈장(PRP)과 빈혈소판 혈장(PPP)이 층을 나뉘어 형성된다. 하측에 위치하게 된 제1 캡(10)을 회전시켜 제1 키트(20)의 상측 공간(20a)의 부피를 적절히 조정한 후, 제1 키트(20)에 대해 스토퍼(30)를 회전시켜 제1 키트(20)의 제1 개구부(24a)를 폐쇄한다. 이렇게 하여 분리된 혈액 중의 PRP를 제1 캡(10)의 내측부(12)를 통해 주사기를 이용하여 추출하게 된다.First, the collected blood is injected into the blood separation device 1 through the opening 25 of the first kit 20 or the opening 46 of the second kit 40. Next, the first opening 24a of the first kit 20 is closed by the first bar 33 of the stopper 30, and the second opening 43a of the second kit 40 is open. The centrifugation is performed first at. When the first centrifugation is completed, the plasma and buffy coat in the blood are located in the upper space 40a of the second kit 40, and the blood cells are located in the lower space 40b. At this time, the second cap 50 is rotated to appropriately adjust the volume of the lower space 40b, thereby accurately separating blood components. Thereafter, the second opening 43a of the second kit 40 is closed by the second bar 34 of the stopper 30. Next, after the blood separation device 1 is turned over, that is, the first kit 20 is positioned below the second kit 40, the first opening 24a of the first kit 20 is opened. Secondary centrifugation is performed in an open state. As a result, multiplatelet plasma (PRP) and anemia platelet plasma (PPP) are formed by dividing the layer. After adjusting the volume of the upper space 20a of the first kit 20 by rotating the first cap 10 positioned at the lower side, the stopper 30 is rotated with respect to the first kit 20. 1 Close the first opening 24a of the kit 20. In this way, the PRP in the separated blood is extracted using a syringe through the inner portion 12 of the first cap 10.
이와 같이 혈액은 혈액 분리 장치(10) 내에서 외부에의 노출 없이 2회 원심 분리 가능하기 때문에, 혈액의 대기 노출에 따른 감염 등의 우려 없이 고농축된 PRP의 추출이 가능하게 된다.As described above, since the blood can be centrifuged twice in the blood separation apparatus 10 without being exposed to the outside, highly concentrated PRP can be extracted without fear of infection due to the air exposure of the blood.
전술한 본 발명의 설명은 예시를 위한 것이며, 본 발명이 속하는 기술분야의 통상의 지식을 가진 자는 본 발명의 기술적 사상이나 필수적인 특징을 변경하지 않고서 다른 구체적인 형태로 쉽게 변형이 가능하다는 것을 이해할 수 있을 것이다. 그러므로 이상에서 기술한 실시예들은 모든 면에서 예시적인 것이며 한정적이 아닌 것으로 이해해야만 한다. 예를 들어, 단일형으로 설명되어 있는 각 구성 요소는 분산되어 실시될 수도 있으며, 마찬가지로 분산된 것으로 설명되어 있는 구성 요소들도 결합된 형태로 실시될 수 있다.The foregoing description of the present invention is intended for illustration, and it will be understood by those skilled in the art that the present invention may be easily modified in other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are exemplary in all respects and not restrictive. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.
본 발명의 범위는 후술하는 특허청구범위에 의하여 나타내어지며, 특허청구범위의 의미 및 범위 그리고 그 균등 개념으로부터 도출되는 모든 변경 또는 변형된 형태가 본 발명의 범위에 포함되는 것으로 해석되어야 한다.The scope of the present invention is represented by the following claims, and it should be construed that all changes or modifications derived from the meaning and scope of the claims and their equivalents are included in the scope of the present invention.

Claims (12)

  1. 내측에 혈액을 수용하는 제1 공간을 포함하며, 상기 제1 공간은 제1 개구부를 통해 상하 분리되어 있는 제1 키트,A first space accommodating blood therein, the first space being firstly divided up and down through a first opening;
    내측에 혈액을 수용하는 제2 공간을 포함하며, 상기 제2 공간은 제2 개구부를 통해 상하 분리되어 있는 제2 키트,A second space accommodating blood therein, wherein the second space is vertically separated through a second opening;
    상기 제1 키트 및 상기 제2 키트의 사이에 배치되며, 상기 제1 공간과 상기 제2 공간을 유체 연통시키는 연통홀이 구비된 플레이트, 상기 플레이트로부터 상측으로 돌출 설치되어 상기 제1 개구부를 개폐하는 제1 바(bar), 및 상기 플레이트로부터 하측으로 돌출 설치되어 상기 제2 개구부를 개폐하는 제2 바를 가지는 스토퍼,A plate disposed between the first kit and the second kit and provided with a communication hole for fluid communication between the first space and the second space, the plate protruding upward from the plate to open and close the first opening; A stopper having a first bar and a second bar protruding downward from the plate to open and close the second opening;
    상기 제1 키트의 상단부를 커버하는 제1 캡, 및A first cap covering an upper end of the first kit, and
    상기 제2 키트의 하단부를 커버하는 제2 캡을 포함하며,A second cap covering a lower end of the second kit,
    상기 스토퍼는 상기 제1 개구부와 상기 제2 개구부를 각각 개폐함으로써 복수 회의 혈액 분리가 가능한 것을 특징으로 하는 혈액 분리 장치.The stopper is a blood separation device, characterized in that a plurality of blood separation is possible by opening and closing the first opening and the second opening, respectively.
  2. 제1항에 있어서,The method of claim 1,
    상기 제1 공간 및 상기 제2 공간은 혈액의 주입 이전에 진공 상태로 유지되는 것을 특징으로 하는 혈액 분리 장치.And the first space and the second space are maintained in a vacuum state prior to the injection of blood.
  3. 제1항에 있어서,The method of claim 1,
    상기 스토퍼는 상기 제1 키트 및 상기 제2 키트와 회전 가능하게 나사 결합되어 상하 이동될 수 있는 것을 특징으로 하는 혈액 분리 장치.The stopper is a blood separation device, characterized in that the screw rotatably screwed with the first kit and the second kit can be moved up and down.
  4. 제1항에 있어서,The method of claim 1,
    상기 제1 키트 또는 상기 제2 키트에는 혈액을 주입 또는 추출하기 위한 개구부가 마련되는 것을 특징으로 하는 혈액 분리 장치.Blood separation device, characterized in that the first kit or the second kit is provided with an opening for injecting or extracting blood.
  5. 제4항에 있어서,The method of claim 4, wherein
    상기 개구부에는 오염방지용 필터가 마련되는 것을 특징으로 하는 혈액 분리 장치.Blood separation device, characterized in that the opening is provided with a filter for preventing contamination.
  6. 제1항에 있어서,The method of claim 1,
    상기 제1 캡은 상기 제1 키트와 회전 가능하게 나사 결합되어 상하 이동될 수 있는 것을 특징으로 하는 혈액 분리 장치.The first cap is a blood separation device, characterized in that the rotatably screwed with the first kit can be moved up and down.
  7. 제1항에 있어서,The method of claim 1,
    상기 제2 캡은 상기 제2 키트와 회전 가능하게 나사 결합되어 상하 이동될 수 있는 것을 특징으로 하는 혈액 분리 장치.The second cap is blood-separated device, characterized in that the screw rotatably screwed with the second kit can be moved up and down.
  8. 제1항 내지 제7항 중 어느 한 항에 있어서,The method according to any one of claims 1 to 7,
    상기 제1 캡 또는 상기 제2 캡은 실리콘 또는 고무 재질의 부재를 포함하는 것을 특징으로 하는 혈액 분리 장치.The first cap or the second cap is a blood separation device, characterized in that it comprises a member made of silicone or rubber.
  9. 제1항 내지 제7항 중 어느 한 항에 있어서,The method according to any one of claims 1 to 7,
    상기 제1 키트, 상기 제2 키트, 상기 스토퍼, 상기 제1 캡, 및 상기 제2 캡 중 하나 이상은 투명한 소재로 된 것을 특징으로 하는 혈액 분리 장치.At least one of the first kit, the second kit, the stopper, the first cap, and the second cap is made of a transparent material.
  10. 제1항 내지 제7항 중 어느 한 항에 있어서,The method according to any one of claims 1 to 7,
    상기 제1 캡 또는 상기 제2 캡에는 눈금선이 표시되어 있는 것을 특징으로 하는 혈액 분리 장치.The blood separation device, characterized in that the grid line is displayed on the first cap or the second cap.
  11. 제1항 내지 제7항 중 어느 한 항에 있어서,The method according to any one of claims 1 to 7,
    상기 제1 키트 또는 상기 제2 키트는 상측 공간 및 하측 공간으로 분리되는 모래시계 형상으로 된 것을 특징으로 하는 혈액 분리 장치.The first kit or the second kit is a blood separation device, characterized in that the hourglass shape is divided into an upper space and a lower space.
  12. 제1항 내지 제7항 중 어느 한 항에 있어서,The method according to any one of claims 1 to 7,
    상기 스토퍼와 상기 제1 키트의 사이, 및 상기 스토퍼와 상기 제2 키트의 사이에는 실리콘 또는 고무 재질의 실링 링이 배치되는 것을 특징으로 하는 혈액 분리 장치.And a sealing ring made of silicon or rubber is disposed between the stopper and the first kit and between the stopper and the second kit.
PCT/KR2012/001235 2011-12-20 2012-02-20 Blood separating device WO2013094819A1 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106669875A (en) * 2017-02-13 2017-05-17 深圳市邦沃科技有限公司 Platelet-rich plasma centrifuge tube and extraction method thereof

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101626373B1 (en) * 2015-02-04 2016-06-01 주식회사 지에스엠코리아 Blood separating device
KR101563512B1 (en) * 2015-04-09 2015-10-28 기 수 한 Blood separating vial
US10413902B2 (en) * 2015-07-17 2019-09-17 Stat-Diagnostica & Innovation, S.L. Apparatus for sample separation and collection
EP3678781B1 (en) * 2017-09-05 2022-05-04 Beckman Coulter, Inc. Collection and preparation of blood samples for point-of-care diagnostics
KR102119835B1 (en) * 2017-11-22 2020-06-05 (주)시지바이오 Centrifuge device for separating blood components
KR101930290B1 (en) * 2018-01-12 2018-12-18 (주)레보메드 Device for Separating the Body Fluid
WO2019210086A1 (en) * 2018-04-25 2019-10-31 Ge Healthcare Bio-Sciences Corp. Storage vial
KR101892197B1 (en) * 2018-06-20 2018-08-28 (주)웰포트 Apparatus for extracting platelet rich plasma
CN108992979B (en) * 2018-07-19 2020-09-15 中国科学院东北地理与农业生态研究所 Reagent exchange storage filtering double-thread-opening combined device
KR101979382B1 (en) * 2018-11-30 2019-05-17 (주)레보메드 Kit for Separating and Concentrating a Body Fluid Cell
KR102527628B1 (en) * 2019-05-20 2023-05-03 (의) 삼성의료재단 Container for tissue adhesiveness
KR102339809B1 (en) * 2021-03-30 2021-12-16 (주)레보메드 Bio kit for extracting bodily fluids containing stem cells
KR102395059B1 (en) * 2021-12-30 2022-05-09 (주) 레보메드 Internal sterile treatment type body fluid cell separation and concentration kit

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008104789A (en) * 2006-10-27 2008-05-08 Nipro Corp Method and device for separation of platelet rich plasma
KR20100105282A (en) * 2009-03-16 2010-09-29 문상호 Seperator and collection vial for platelet rich plasma
KR101008583B1 (en) * 2010-10-27 2011-01-17 주식회사 메디사랑 Filtration device for the separation of platelet rich fibrin
KR101026599B1 (en) * 2010-12-30 2011-04-04 문상호 Filtration device for the separation of platelet rich plsama
US20110281714A1 (en) * 2005-02-07 2011-11-17 Biomet Biologics, Llc Method and Apparatus for Preparing Platelet Rich Plasma and Concentrates Thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4131549A (en) * 1977-05-16 1978-12-26 Ferrara Louis T Serum separation device
WO2001083068A1 (en) * 2000-04-28 2001-11-08 Harvest Technologies Corporation Blood components separator disk
CN2429826Y (en) * 2000-05-29 2001-05-09 北京伏尔特技术有限公司 Blood separator
AU2004242150B2 (en) * 2003-05-19 2008-01-10 Harvest Technologies Corporation Method and apparatus for separating fluid components
KR101289535B1 (en) * 2009-12-07 2013-07-24 전민용 Centrifuge tube

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110281714A1 (en) * 2005-02-07 2011-11-17 Biomet Biologics, Llc Method and Apparatus for Preparing Platelet Rich Plasma and Concentrates Thereof
JP2008104789A (en) * 2006-10-27 2008-05-08 Nipro Corp Method and device for separation of platelet rich plasma
KR20100105282A (en) * 2009-03-16 2010-09-29 문상호 Seperator and collection vial for platelet rich plasma
KR101008583B1 (en) * 2010-10-27 2011-01-17 주식회사 메디사랑 Filtration device for the separation of platelet rich fibrin
KR101026599B1 (en) * 2010-12-30 2011-04-04 문상호 Filtration device for the separation of platelet rich plsama

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106669875A (en) * 2017-02-13 2017-05-17 深圳市邦沃科技有限公司 Platelet-rich plasma centrifuge tube and extraction method thereof
CN106669875B (en) * 2017-02-13 2022-07-01 深圳市邦沃科技有限公司 Platelet-rich plasma centrifuge tube and extraction method thereof

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