WO2013053471A3 - Device and method for pressurized cryopreservation of a biological sample - Google Patents

Device and method for pressurized cryopreservation of a biological sample Download PDF

Info

Publication number
WO2013053471A3
WO2013053471A3 PCT/EP2012/004248 EP2012004248W WO2013053471A3 WO 2013053471 A3 WO2013053471 A3 WO 2013053471A3 EP 2012004248 W EP2012004248 W EP 2012004248W WO 2013053471 A3 WO2013053471 A3 WO 2013053471A3
Authority
WO
WIPO (PCT)
Prior art keywords
pressure
biological sample
cryopreservation
pressure vessel
maintaining
Prior art date
Application number
PCT/EP2012/004248
Other languages
German (de)
French (fr)
Other versions
WO2013053471A2 (en
Inventor
Günter R. FUHR
Heiko Zimmermann
Frank Stracke
Markus GRABENBAUER
Jan HÜBINGER
Phillipe BASTIAENS
Frank Wehner
Original Assignee
Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V., MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. filed Critical Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
Priority to US14/351,136 priority Critical patent/US20140260346A1/en
Priority to EP12772885.5A priority patent/EP2765854A2/en
Publication of WO2013053471A2 publication Critical patent/WO2013053471A2/en
Publication of WO2013053471A3 publication Critical patent/WO2013053471A3/en

Links

Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D31/00—Other cooling or freezing apparatus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
    • A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0278—Physical preservation processes
    • A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0278—Physical preservation processes
    • A01N1/0289—Pressure processes, i.e. using a designated change in pressure over time

Abstract

The invention concerns a method and devices for the cryopreservation of a biological sample (1), which comprises biological cells and a preservation medium, it being possible for different temperature and pressure time functions to be implemented. The device (100) comprises a pressure vessel (10) which is arranged for accommodating the biological sample and can be acted upon by a pressure which is higher than the ambient atmospheric pressure. To that end, the pressure vessel comprises at least one pressure-adjusting element in the form of a pressure screw (21), expansion region or pressure clamp. The pressure vessel is arranged to be immersed in a fluid bath of a cooling device. Methods are also described for vitality-maintaining warming of the biological sample. At least one stabilizer substance (e.g. ethylene glycol, Ficoll, Percoll) can be provided as component of the preservation medium, which is suitable for maintaining a vitreous phase when the temperature of the biological sample is increased until transition to a fluid state.
PCT/EP2012/004248 2011-10-10 2012-10-10 Device and method for high-pressure cryopreservation of a biological sample WO2013053471A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US14/351,136 US20140260346A1 (en) 2011-10-10 2012-10-10 Device and method for pressurized cryopreservation of a biological sample
EP12772885.5A EP2765854A2 (en) 2011-10-10 2012-10-10 Device and method for high-pressure cryopreservation of a biological sample

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102011115467A DE102011115467A1 (en) 2011-10-10 2011-10-10 Apparatus and method for pressure-cryopreserving a biological sample
DE102011115467.5 2011-10-10

Publications (2)

Publication Number Publication Date
WO2013053471A2 WO2013053471A2 (en) 2013-04-18
WO2013053471A3 true WO2013053471A3 (en) 2013-08-22

Family

ID=47022625

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2012/004248 WO2013053471A2 (en) 2011-10-10 2012-10-10 Device and method for high-pressure cryopreservation of a biological sample

Country Status (4)

Country Link
US (1) US20140260346A1 (en)
EP (1) EP2765854A2 (en)
DE (1) DE102011115467A1 (en)
WO (1) WO2013053471A2 (en)

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WO2015148935A1 (en) 2014-03-28 2015-10-01 Board Of Trustees Of Northern Illinois University Ultra-rapid tissue cryopreservation method and apparatus
EP3142487B1 (en) * 2014-05-13 2019-07-03 Assuta Medical Centers Ltd Cell tray
JP6966050B2 (en) * 2015-10-29 2021-11-10 アシンプトート リミテッドAsymptote Ltd Methods for cryopreservation
DE102016005078A1 (en) 2016-04-27 2017-11-02 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Apparatus and method for monitoring the temperature of a cryopreserved biological sample
DE102016005070A1 (en) 2016-04-27 2017-11-02 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Apparatus and method for monitoring the temperature of a cryopreserved biological sample
DE102016005133A1 (en) 2016-04-27 2017-11-02 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Method and device for monitoring the temperature of a cryopreserved biological sample
DE102016005075A1 (en) 2016-04-27 2017-11-02 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Method and device for monitoring the temperature of a cryopreserved biological sample
FR3062284B1 (en) * 2017-01-30 2020-10-09 Genialis PROCESS FOR COOLING A BIOLOGICAL MATERIAL AND ITS CONSERVATION
GB201705652D0 (en) * 2017-04-07 2017-05-24 Asymptote Ltd Cryopreservation apparatus and methods
RU2688331C1 (en) * 2018-04-02 2019-05-21 Российская Федерация, от имени которой выступает ФОНД ПЕРСПЕКТИВНЫХ ИССЛЕДОВАНИЙ Method for cryopreservation of biological samples under pressure and device for its implementation
CN109964921A (en) * 2019-02-25 2019-07-05 天津美电医疗科技有限公司 Temperature below freezing saves the multiphase volume fixing device of biological substance, system and method
CN109997841B (en) * 2019-04-09 2021-02-02 上海交通大学 Human gamete rapid freezing and thawing visualization environment cabin maintaining atmospheric air atmosphere
US20210000104A1 (en) * 2019-07-05 2021-01-07 CryoStasis Ltd. Methods, systems and apparatus for preservation of organs and other aqueous-based materials utilizing low temperature and elevated pressure

Citations (6)

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US4559298A (en) * 1982-11-23 1985-12-17 American National Red Cross Cryopreservation of biological materials in a non-frozen or vitreous state
EP0232672A1 (en) * 1985-11-12 1987-08-19 Vital Force, Inc. A method for preservation and storage of viable biological materials at cryogenic temperatures
DE19826350A1 (en) * 1998-06-12 1999-12-23 Schmid Schoenbein Holger Preservation of blood cells, especially red blood cells
DE10025512A1 (en) * 1999-07-06 2001-01-11 Leica Mikrosysteme Ag Wien High pressure freezing device for attainment of fine crystalline or amorphous state with rapid freezing of water-containing substances, especially biological probes
US20070087321A1 (en) * 2003-09-09 2007-04-19 Csaba Pribenszky Post-thaw survival of chryopreserved biological material by hydrostatic pressure challenge
US20100184012A1 (en) * 2006-04-04 2010-07-22 Voelker Mark A Methods and devices for imaging and manipulating biological samples

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US4559298A (en) * 1982-11-23 1985-12-17 American National Red Cross Cryopreservation of biological materials in a non-frozen or vitreous state
EP0232672A1 (en) * 1985-11-12 1987-08-19 Vital Force, Inc. A method for preservation and storage of viable biological materials at cryogenic temperatures
DE19826350A1 (en) * 1998-06-12 1999-12-23 Schmid Schoenbein Holger Preservation of blood cells, especially red blood cells
DE10025512A1 (en) * 1999-07-06 2001-01-11 Leica Mikrosysteme Ag Wien High pressure freezing device for attainment of fine crystalline or amorphous state with rapid freezing of water-containing substances, especially biological probes
US20070087321A1 (en) * 2003-09-09 2007-04-19 Csaba Pribenszky Post-thaw survival of chryopreserved biological material by hydrostatic pressure challenge
US20100184012A1 (en) * 2006-04-04 2010-07-22 Voelker Mark A Methods and devices for imaging and manipulating biological samples

Non-Patent Citations (3)

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Title
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Also Published As

Publication number Publication date
US20140260346A1 (en) 2014-09-18
WO2013053471A2 (en) 2013-04-18
EP2765854A2 (en) 2014-08-20
DE102011115467A1 (en) 2013-04-11

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