WO2013042120A3 - Synthetic ubiquitins and use thereof in drug screening assays - Google Patents

Synthetic ubiquitins and use thereof in drug screening assays Download PDF

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Publication number
WO2013042120A3
WO2013042120A3 PCT/IL2012/050377 IL2012050377W WO2013042120A3 WO 2013042120 A3 WO2013042120 A3 WO 2013042120A3 IL 2012050377 W IL2012050377 W IL 2012050377W WO 2013042120 A3 WO2013042120 A3 WO 2013042120A3
Authority
WO
WIPO (PCT)
Prior art keywords
ubiquitins
synthetic
screening assays
drug screening
amino acid
Prior art date
Application number
PCT/IL2012/050377
Other languages
French (fr)
Other versions
WO2013042120A2 (en
Inventor
Philippe Nakache
Omri Erez
Danny Ben-Avraham
Original Assignee
Proteologics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Proteologics Ltd filed Critical Proteologics Ltd
Publication of WO2013042120A2 publication Critical patent/WO2013042120A2/en
Publication of WO2013042120A3 publication Critical patent/WO2013042120A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof

Abstract

Synthetic ubiquitins are provided in which at least one amino acid residue is replaced with a residue of an alpha-amino acid substituted by a detectable moiety, useful in various high throughput screening (HTS) methods for identifying potential drug candidates as inhibitors of ubiquitin-mediated proteolysis.
PCT/IL2012/050377 2011-09-19 2012-09-19 Synthetic ubiquitins and use thereof in drug screening assays WO2013042120A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161536428P 2011-09-19 2011-09-19
US61/536,428 2011-09-19

Publications (2)

Publication Number Publication Date
WO2013042120A2 WO2013042120A2 (en) 2013-03-28
WO2013042120A3 true WO2013042120A3 (en) 2013-09-19

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2012/050377 WO2013042120A2 (en) 2011-09-19 2012-09-19 Synthetic ubiquitins and use thereof in drug screening assays

Country Status (1)

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WO (1) WO2013042120A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104280366B (en) * 2013-07-03 2017-10-10 复旦大学 A kind of method of the target proteinses of high flux detection compound
CN110835641B (en) * 2019-10-23 2023-04-07 中山大学肿瘤防治中心（中山大学附属肿瘤医院、中山大学肿瘤研究所） Nanobret-based protein ubiquitination degradation promoting drug screening system and method
CN112649605B (en) * 2020-12-14 2022-11-01 哈尔滨理工大学 Based on NaBiF4ECL biosensor for up-conversion of nanoparticles

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4927923A (en) * 1984-09-26 1990-05-22 Compagnie Oris Industries Macropolycyclic rare earth complexes and application as fluorescent tracers
US5162508A (en) * 1987-12-18 1992-11-10 Compagnie Oris Industrie Rare earth cryptates, processes for their preparation, synthesis intermediates and application as fluorescent tracers
US20060063221A1 (en) * 2004-09-21 2006-03-23 Williams Dudley J Lanthanide-based substrates and methods for determining clostridial toxin activity
WO2006060739A2 (en) * 2004-12-01 2006-06-08 Proteologics, Inc. Ubiquitin ligase assays and related reagents
WO2007018315A1 (en) * 2005-08-09 2007-02-15 Japan Science And Technology Agency Protein probe for use in detection of interaction between the protein and other molecule
US20110224411A1 (en) * 2005-11-14 2011-09-15 Japan Science And Technology Agency Mutant tRNA for Introducing Unnatural Amino Acid Into Protein

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6740495B1 (en) 2000-04-03 2004-05-25 Rigel Pharmaceuticals, Inc. Ubiquitin ligase assay

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4927923A (en) * 1984-09-26 1990-05-22 Compagnie Oris Industries Macropolycyclic rare earth complexes and application as fluorescent tracers
US5162508A (en) * 1987-12-18 1992-11-10 Compagnie Oris Industrie Rare earth cryptates, processes for their preparation, synthesis intermediates and application as fluorescent tracers
US20060063221A1 (en) * 2004-09-21 2006-03-23 Williams Dudley J Lanthanide-based substrates and methods for determining clostridial toxin activity
WO2006060739A2 (en) * 2004-12-01 2006-06-08 Proteologics, Inc. Ubiquitin ligase assays and related reagents
WO2007018315A1 (en) * 2005-08-09 2007-02-15 Japan Science And Technology Agency Protein probe for use in detection of interaction between the protein and other molecule
US20110224411A1 (en) * 2005-11-14 2011-09-15 Japan Science And Technology Agency Mutant tRNA for Introducing Unnatural Amino Acid Into Protein

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
EDUARDO J. A. OLIVIERA: "A FLUORESCENT-LABELED MICROSYSTIN-LR TERBIUM CRYPTATE", J. BRAZ. CHEM. SOC., 30 April 2006 (2006-04-30), pages 243 - 250, XP055067951 *
MICHAEL WEN-PIN KAO ET AL: "Strategy for Efficient Site-Specific FRET-Dye Labeling of Ubiquitin", BIOCONJUGATE CHEMISTRY, vol. 19, no. 6, 1 June 2008 (2008-06-01), pages 1124 - 1126, XP055055467, ISSN: 1043-1802, DOI: 10.1021/bc700480j *
SHETTY D ET AL: "Synthesis of novel <68>Ga-labeled amino acid derivatives for positron emission tomography of cancer cells", NUCLEAR MEDICINE AND BIOLOGY, ELSEVIER, NY, US, vol. 37, no. 8, 1 November 2010 (2010-11-01), pages 893 - 902, XP027472061, ISSN: 0969-8051, [retrieved on 20101101], DOI: 10.1016/J.NUCMEDBIO.2010.06.003 *

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