WO2010087994A3 - Methods for ligation and uses thereof - Google Patents

Methods for ligation and uses thereof Download PDF

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Publication number
WO2010087994A3
WO2010087994A3 PCT/US2010/000274 US2010000274W WO2010087994A3 WO 2010087994 A3 WO2010087994 A3 WO 2010087994A3 US 2010000274 W US2010000274 W US 2010000274W WO 2010087994 A3 WO2010087994 A3 WO 2010087994A3
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WO
WIPO (PCT)
Prior art keywords
ligation
methods
acyl
acyl donor
transamidase
Prior art date
Application number
PCT/US2010/000274
Other languages
French (fr)
Other versions
WO2010087994A2 (en
Inventor
Hidde L. Ploegh
John M. Antos
Maximilian Wei-Lin Popp
Carla Guimaraes
Original Assignee
Whitehead Institute For Biomedical Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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Application filed by Whitehead Institute For Biomedical Research filed Critical Whitehead Institute For Biomedical Research
Priority to EP10736161.0A priority Critical patent/EP2391714B2/en
Priority to US13/145,644 priority patent/US8940501B2/en
Publication of WO2010087994A2 publication Critical patent/WO2010087994A2/en
Publication of WO2010087994A3 publication Critical patent/WO2010087994A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals

Abstract

The present invention relates to methods for ligation. The invention provides novel reagents and methods for ligating an acyl donor compound with an acyl acceptor compound. Provided acyl donor compounds comprise a transamidase recognition sequence that allows ligation with a nucleophilic acyl acceptor in the presence of transamidase. The invention further provides kits comprising acyl donor compounds and optionally comprising other reagents for ligation.
PCT/US2010/000274 2009-01-30 2010-02-01 Methods for ligation and uses thereof WO2010087994A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP10736161.0A EP2391714B2 (en) 2009-01-30 2010-02-01 Methods for ligation and uses thereof
US13/145,644 US8940501B2 (en) 2009-01-30 2010-02-01 Methods for ligation and uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14878709P 2009-01-30 2009-01-30
US61/148,787 2009-01-30

Publications (2)

Publication Number Publication Date
WO2010087994A2 WO2010087994A2 (en) 2010-08-05
WO2010087994A3 true WO2010087994A3 (en) 2011-02-17

Family

ID=42396266

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/000274 WO2010087994A2 (en) 2009-01-30 2010-02-01 Methods for ligation and uses thereof

Country Status (3)

Country Link
US (1) US8940501B2 (en)
EP (1) EP2391714B2 (en)
WO (1) WO2010087994A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105555794A (en) * 2013-07-10 2016-05-04 哈佛学院院长及董事 Compositions and methods relating to nucleic acid-protein complexes
CN107001446A (en) * 2014-12-17 2017-08-01 豪夫迈·罗氏有限公司 Using the double conjugation of polypeptides enzymatic one pot reactions of the single stage of sorting enzyme

Families Citing this family (82)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8940501B2 (en) 2009-01-30 2015-01-27 Whitehead Institute For Biomedical Research Methods for ligation and uses thereof
WO2010115589A1 (en) 2009-04-07 2010-10-14 Roche Glycart Ag Trivalent, bispecific antibodies
US9676845B2 (en) 2009-06-16 2017-06-13 Hoffmann-La Roche, Inc. Bispecific antigen binding proteins
RU2573915C2 (en) 2009-09-16 2016-01-27 Дженентек, Инк. Protein complexes containing superhelix and/or banding, and their use
AR080793A1 (en) 2010-03-26 2012-05-09 Roche Glycart Ag BISPECIFIC ANTIBODIES
WO2012025530A1 (en) 2010-08-24 2012-03-01 F. Hoffmann-La Roche Ag Bispecific antibodies comprising a disulfide stabilized - fv fragment
WO2012085111A1 (en) 2010-12-23 2012-06-28 F. Hoffmann-La Roche Ag Polypeptide-polynucleotide-complex and its use in targeted effector moiety delivery
JP5768147B2 (en) 2011-02-28 2015-08-26 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Monovalent antigen binding protein
KR101638224B1 (en) 2011-02-28 2016-07-08 에프. 호프만-라 로슈 아게 Antigen binding proteins
CN103764665A (en) 2011-06-28 2014-04-30 怀特黑德生物医学研究所 Using sortases to install click chemistry handles for protein ligation
US9588110B2 (en) 2011-07-28 2017-03-07 Cell Signaling Technology, Inc. Multi component antibody based detection technology
WO2013065009A1 (en) * 2011-11-01 2013-05-10 National Institute Of Immunology A sortase-click reaction suite for synthesis of multivalent dendrimeric protein assembly
WO2013095091A2 (en) 2011-11-17 2013-06-27 Aimm Therapeutics B.V. Rsv g protein specific antibodies
KR20140112495A (en) 2011-12-02 2014-09-23 에임 쎄라퓨틱스 비.브이. Influenza a virus specific antibodies
BR112014019579A2 (en) 2012-02-10 2019-10-15 Genentech, Inc SINGLE CHAIN ANTIBODY, POLYNUCLEOTIDE, VECTOR, HOST CELL, METHOD OF PRODUCTION OF A SINGLE CHAIN ANTIBODY, HETEROMULTYMER AND METHOD OF PRODUCTION
DK2814829T3 (en) 2012-02-13 2017-03-20 Bristol Myers Squibb Co RELATIONSHIPS, CONJUGATES THEREOF AND USES AND RELATED PROCEDURES
WO2013124473A1 (en) 2012-02-24 2013-08-29 Novartis Ag Pilus proteins and compositions
EP2830991A1 (en) 2012-03-26 2015-02-04 President and Fellows of Harvard College Lipid-coated nucleic acid nanostructures of defined shape
US8722354B2 (en) 2012-04-03 2014-05-13 Empire Technology Development Llc Fluorescent labeling of living cells
US9267127B2 (en) 2012-06-21 2016-02-23 President And Fellows Of Harvard College Evolution of bond-forming enzymes
CA2871882A1 (en) 2012-06-27 2014-01-03 F. Hoffmann-La Roche Ag Method for making antibody fc-region conjugates comprising at least one binding entity that specifically binds to a target and uses thereof
BR112014032193A2 (en) 2012-06-27 2017-06-27 Hoffmann La Roche bispecific antibody production and combination determination methods, bispecific antibody, formulation and use of bispecific antibody
CN104619715B (en) * 2012-09-14 2018-06-05 弗·哈夫曼-拉罗切有限公司 The production of molecule comprising at least two different entities and selection method and application thereof
US20150284477A1 (en) * 2012-10-30 2015-10-08 President and Fellows of Haverd College Sortase-catalyzed immobilization, release, and replacement of functional molecules on solid surfaces
EP2733153A1 (en) * 2012-11-15 2014-05-21 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for the preparation of immunoconjugates and uses thereof
LT2956173T (en) 2013-02-14 2017-06-26 Bristol-Myers Squibb Company Tubulysin compounds, methods of making and use
EP2777714A1 (en) * 2013-03-15 2014-09-17 NBE-Therapeutics LLC Method of producing an immunoligand/payload conjugate by means of a sequence-specific transpeptidase enzyme
KR101808223B1 (en) * 2013-04-25 2017-12-13 주식회사 앱틀라스 Method for refining protein including self-cutting cassette and use thereof
US10260038B2 (en) 2013-05-10 2019-04-16 Whitehead Institute For Biomedical Research Protein modification of living cells using sortase
US10471099B2 (en) 2013-05-10 2019-11-12 Whitehead Institute For Biomedical Research In vitro production of red blood cells with proteins comprising sortase recognition motifs
EA201690388A1 (en) 2013-08-14 2016-07-29 Уильям Марш Райс Юниверсити NEW DERIVATIVES OF UNCIALAMICINE, METHODS OF THEIR SYNTHESIS AND THEIR APPLICATION AS ANTI-TUMOR AGENTS
WO2015037992A1 (en) 2013-09-11 2015-03-19 Aimm Therapeutics B.V. Parechovirus specific antibodies
WO2015042393A2 (en) 2013-09-20 2015-03-26 President And Fellows Of Harvard College Evolved sortases and uses thereof
MX2016003593A (en) 2013-10-11 2016-06-02 Hoffmann La Roche Multispecific domain exchanged common variable light chain antibodies.
US10556024B2 (en) 2013-11-13 2020-02-11 Whitehead Institute For Biomedical Research 18F labeling of proteins using sortases
DK3083678T3 (en) 2013-12-17 2019-06-17 Aimm Therapeutics Bv Means and Procedures for Disinfecting Myeloproliferative or Lymphoproliferative Disorders
EP3517114A1 (en) 2014-04-29 2019-07-31 Genequantum Healthcare (Suzhou) Co., Ltd. New stable antibody-drug conjugate, preparation method and use thereof
WO2016014553A1 (en) 2014-07-21 2016-01-28 Novartis Ag Sortase synthesized chimeric antigen receptors
US10053683B2 (en) 2014-10-03 2018-08-21 Whitehead Institute For Biomedical Research Intercellular labeling of ligand-receptor interactions
US10077287B2 (en) 2014-11-10 2018-09-18 Bristol-Myers Squibb Company Tubulysin analogs and methods of making and use
WO2016087416A1 (en) 2014-12-03 2016-06-09 F. Hoffmann-La Roche Ag Multispecific antibodies
WO2016096741A1 (en) 2014-12-17 2016-06-23 F. Hoffmann-La Roche Ag Novel methods for enzyme mediated polypeptide conjugation using sortase
EP3234177A1 (en) 2014-12-17 2017-10-25 F. Hoffmann-La Roche AG Activity assay for bond forming enzymes
EA201791359A1 (en) 2014-12-23 2017-11-30 ЭнБиИ-ТЕРАПЬЮТИКС АГ CONJUGATES CONNECTING THE PROTEIN-MEDICINAL CONTAINING CONTAINING ANTHRACYCLINE DERIVATIVES
ES2747386T3 (en) 2015-01-14 2020-03-10 Bristol Myers Squibb Co Heteroarylene-linked benzodiazepine dimers, conjugates thereof and methods of preparation and use
WO2016167291A1 (en) 2015-04-13 2016-10-20 国立研究開発法人産業技術総合研究所 Cyclized cytokine and method for producing same
JP6998862B2 (en) 2015-09-25 2022-02-04 エフ．ホフマン－ラ ロシュ アーゲー Soluble sortase A
CN108026560A (en) 2015-09-25 2018-05-11 豪夫迈·罗氏有限公司 Reacted in eutectic solvent using the acid amides that turns of sorting enzyme
WO2017050889A1 (en) 2015-09-25 2017-03-30 F. Hoffmann-La Roche Ag Recombinant immunoglobulin heavy chains comprising a sortase conjugation loop and conjugates thereof
WO2017050874A1 (en) 2015-09-25 2017-03-30 F. Hoffmann-La Roche Ag Process for producing thioesters employing a sortase a
US20180280551A1 (en) 2015-10-01 2018-10-04 Whitehead Institute For Biomedical Research Labeling of antibodies
US11136407B2 (en) 2016-01-08 2021-10-05 Aimm Therapeutics B.V. Therapeutic anti-CD9 antibody
WO2017132395A1 (en) 2016-01-26 2017-08-03 The Regents Of The University Of California Methods and compositions to increase the rate of ligation reactions catalyzed by a sortase
EP3417058B1 (en) 2016-02-16 2021-09-22 Research Development Foundation Sortase-modified molecules and uses thereof
US10857234B2 (en) 2016-03-16 2020-12-08 Endocyte Inc. Carbonic anhydrase IX inhibitor conjugates and uses thereof
AU2017234676A1 (en) 2016-03-16 2018-09-20 Purdue Research Foundation Carbonic anhydrase IX targeting agents and methods
WO2017167712A1 (en) 2016-03-30 2017-10-05 F. Hoffmann-La Roche Ag Improved sortase
WO2018017442A1 (en) * 2016-07-18 2018-01-25 President And Fellows Of Harvard College Methods and compositions relating to covalently circularized nanodiscs
KR102493853B1 (en) 2016-08-19 2023-01-30 브리스톨-마이어스 스큅 컴퍼니 Seco-cyclopropapyrroloindole compounds, antibody-drug conjugates thereof, and methods of manufacture and use
WO2018075842A1 (en) 2016-10-20 2018-04-26 Bristol-Myers Squibb Company Condensed benzodiazepine derivatives and conjugates made therefrom
US11560384B2 (en) 2017-05-04 2023-01-24 University Of Utah Research Foundation Benzonorbornadiene derivatives and reactions thereof
US10472361B2 (en) 2017-08-16 2019-11-12 Bristol-Myers Squibb Company Toll-like receptor 7 (TLR7) agonists having a benzotriazole moiety, conjugates thereof, and methods and uses therefor
US10508115B2 (en) 2017-08-16 2019-12-17 Bristol-Myers Squibb Company Toll-like receptor 7 (TLR7) agonists having heteroatom-linked aromatic moieties, conjugates thereof, and methods and uses therefor
US10457681B2 (en) 2017-08-16 2019-10-29 Bristol_Myers Squibb Company Toll-like receptor 7 (TLR7) agonists having a tricyclic moiety, conjugates thereof, and methods and uses therefor
US10487084B2 (en) 2017-08-16 2019-11-26 Bristol-Myers Squibb Company Toll-like receptor 7 (TLR7) agonists having a heterobiaryl moiety, conjugates thereof, and methods and uses therefor
US10494370B2 (en) 2017-08-16 2019-12-03 Bristol-Myers Squibb Company Toll-like receptor 7 (TLR7) agonists having a pyridine or pyrazine moiety, conjugates thereof, and methods and uses therefor
US11485741B2 (en) 2018-04-24 2022-11-01 Bristol-Myers Squibb Company Macrocyclic toll-like receptor 7 (TLR7) agonists
JP7427253B2 (en) 2018-07-09 2024-02-05 国立大学法人 熊本大学 circular single chain antibody
US11554120B2 (en) 2018-08-03 2023-01-17 Bristol-Myers Squibb Company 1H-pyrazolo[4,3-d]pyrimidine compounds as toll-like receptor 7 (TLR7) agonists and methods and uses therefor
JP2022501009A (en) * 2018-09-21 2022-01-06 イノベント バイオロジックス （スウツォウ） カンパニー，リミテッド New interleukin 2 and its use
AU2019363713A1 (en) 2018-10-24 2021-05-27 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Vaccination and antibody generation platform
JP2022524537A (en) * 2019-03-13 2022-05-06 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング Process for the preparation of lipid-added proteinaceous structures
JP2023509973A (en) 2020-01-10 2023-03-10 クリング・バイオセラピューティックス・べー・フェー Epithelial cadherin-specific antibody
US20240077502A1 (en) 2021-01-11 2024-03-07 The Broad Institute, Inc. Amyloid protein modifying sortases and uses thereof
AU2021423664A1 (en) 2021-01-28 2023-09-07 Genequantum Healthcare (Suzhou) Co., Ltd. Ligase fusion proteins and applications thereof
EP4284810A2 (en) * 2021-01-28 2023-12-06 Institute For Cancer Research d/b/a The Research Institute of Fox Chase Cancer Center Treatment of integrin-related disorders with stapled peptides
CN115369127A (en) * 2021-05-18 2022-11-22 苏州有诺真生物科技有限公司 Preparation method of genetically modified vertebrate antibody carrying universal fixed-point coupling interface
WO2023057935A1 (en) * 2021-10-06 2023-04-13 Seqirus Inc. Lipid nanoparticle comprising a nucleic acid-binding protein
US11814394B2 (en) 2021-11-16 2023-11-14 Genequantum Healthcare (Suzhou) Co., Ltd. Exatecan derivatives, linker-payloads, and conjugates and thereof
WO2023175016A1 (en) 2022-03-15 2023-09-21 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Method for producing delivery vesicles
WO2023248125A1 (en) 2022-06-20 2023-12-28 Crispr Therapeutics Ag Cd117-targeting nanoparticles for use in drug delivery
WO2024012566A2 (en) 2022-07-15 2024-01-18 Genequantum Healthcare (Suzhou) Co., Ltd. Antibody, linkers, payload, conjugates and applications thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030022178A1 (en) * 1999-04-15 2003-01-30 Olaf Schneewind Identification of sortase gene
WO2005051976A2 (en) * 2003-11-20 2005-06-09 Ansata Therapeutics, Inc. Protein and peptide ligation processes and one-step purification processes
US20090088372A1 (en) * 2006-03-22 2009-04-02 National Institute Of Immunology Novel Bioconjugates As Therapeutic Agent and Synthesis Thereof

Family Cites Families (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4469863A (en) 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US5225539A (en) 1986-03-27 1993-07-06 Medical Research Council Recombinant altered antibodies and methods of making altered antibodies
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US5096815A (en) 1989-01-06 1992-03-17 Protein Engineering Corporation Generation and selection of novel dna-binding proteins and polypeptides
US5198346A (en) 1989-01-06 1993-03-30 Protein Engineering Corp. Generation and selection of novel DNA-binding proteins and polypeptides
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5859205A (en) 1989-12-21 1999-01-12 Celltech Limited Humanised antibodies
US6316003B1 (en) 1989-12-21 2001-11-13 Whitehead Institute For Biomedical Research Tat-derived transport polypeptides
US5321131A (en) 1990-03-08 1994-06-14 Hybridon, Inc. Site-specific functionalization of oligodeoxynucleotides for non-radioactive labelling
US5608039A (en) 1990-10-12 1997-03-04 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Single chain B3 antibody fusion proteins and their uses
US6099842A (en) 1990-12-03 2000-08-08 The United States Of America As Represented By The Department Of Health And Human Services Recombinant immunotoxin composed of a single chain antibody reacting with the human transferrin receptor and diptheria toxin
DK51092D0 (en) 1991-05-24 1992-04-15 Ole Buchardt OLIGONUCLEOTIDE ANALOGUE DESCRIBED BY PEN, MONOMERIC SYNTHONES AND PROCEDURES FOR PREPARING THEREOF, AND APPLICATIONS THEREOF
US5719262A (en) 1993-11-22 1998-02-17 Buchardt, Deceased; Ole Peptide nucleic acids having amino acid side chains
US5700922A (en) 1991-12-24 1997-12-23 Isis Pharmaceuticals, Inc. PNA-DNA-PNA chimeric macromolecules
US5296703A (en) 1992-04-01 1994-03-22 The Regents Of The University Of California Scanning confocal microscope using fluorescence detection
EP0652890B1 (en) 1992-07-27 1998-01-14 HYBRIDON, Inc. Oligonucleotide alkylphosphonothioates
US5283433A (en) 1992-10-05 1994-02-01 The Regents Of The University Of California Scanning confocal microscope providing a continuous display
WO1994017091A2 (en) 1993-01-21 1994-08-04 Hybridon Inc. Foldback triplex-forming oligonucleotides
US5637684A (en) 1994-02-23 1997-06-10 Isis Pharmaceuticals, Inc. Phosphoramidate and phosphorothioamidate oligomeric compounds
WO1995023865A1 (en) 1994-03-03 1995-09-08 Genentech, Inc. Anti-il-8 monoclonal antibodies for treatment of inflammatory disorders
CA2194907A1 (en) 1994-07-13 1996-02-01 Kouji Matsushima Reshaped human antibody against human interleukin-8
MX9708500A (en) * 1995-05-04 1998-02-28 Scripps Research Inst Synthesis of proteins by native chemical ligation.
US5955599A (en) 1995-06-01 1999-09-21 Hybridon, Inc. Process for making oligonucleotides containing o- and s- methylphosphotriester internucleoside linkages
US6140482A (en) 1995-06-01 2000-10-31 Hybridon, Inc. Primary phosphoramidate internucleoside linkages and oligonucleotides containing the same
US5614622A (en) 1995-06-01 1997-03-25 Hybridon, Inc. 5-pentenoyl moiety as a nucleoside-amino protecting group, 4-pentenoyl-protected nucleotide synthons, and related oligonucleotide syntheses
US5962674A (en) 1995-06-01 1999-10-05 Hybridon, Inc. Synthesis of oligonucleotides containing alkylphosphonate internucleoside linkages
US5637683A (en) 1995-07-13 1997-06-10 Cornell Research Foundation, Inc. Nucleic acid analog with amide linkage and method of making that analog
US5652356A (en) 1995-08-17 1997-07-29 Hybridon, Inc. Inverted chimeric and hybrid oligonucleotides
US6022688A (en) 1996-05-13 2000-02-08 Sequenom, Inc. Method for dissociating biotin complexes
US5766905A (en) 1996-06-14 1998-06-16 Associated Universities Inc. Cytoplasmic bacteriophage display system
NZ333346A (en) 1996-06-28 2000-03-27 Caliper Techn Corp High-throughput screening assay systems in microscale fluidic devices
WO1998008858A1 (en) 1996-08-26 1998-03-05 Hybridon, Inc. Improved reagents and process for synthesis of oligonucleotides containing phosphorodithioate internucleoside linkages
US5886165A (en) 1996-09-24 1999-03-23 Hybridon, Inc. Mixed backbone antisense oligonucleotides containing 2'-5'-ribonucleotide- and 3'-5'-deoxyribonucleotides segments
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US5900481A (en) 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
US6447727B1 (en) 1996-11-19 2002-09-10 Caliper Technologies Corp. Microfluidic systems
JP4358307B2 (en) * 1996-12-24 2009-11-04 ザ スクリップス リサーチ インスティテュート General chemical bond
US6090919A (en) 1997-01-31 2000-07-18 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
US6235471B1 (en) 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
US5739314A (en) 1997-04-25 1998-04-14 Hybridon, Inc. Method for synthesizing 2'-O-substituted pyrimidine nucleosides
BR9812267B1 (en) 1997-07-14 2013-11-26 Recombinant Growth Hormone.
US6100541A (en) 1998-02-24 2000-08-08 Caliper Technologies Corporation Microfluidic devices and systems incorporating integrated optical elements
US6455263B2 (en) 1998-03-24 2002-09-24 Rigel Pharmaceuticals, Inc. Small molecule library screening using FACS
US6461813B2 (en) 1998-09-21 2002-10-08 Rigel Pharmaceuticals, Inc. Multiparameter FACS assays to detect alterations in cell cycle regulation
US6639059B1 (en) 1999-03-24 2003-10-28 Exiqon A/S Synthesis of [2.2.1]bicyclo nucleosides
US7101692B2 (en) 1999-04-15 2006-09-05 The Regents Of The University Of California Identification of sortase gene
US6342221B1 (en) 1999-04-28 2002-01-29 Board Of Regents, The University Of Texas System Antibody conjugate compositions for selectively inhibiting VEGF
KR20010001577A (en) 1999-06-07 2001-01-05 윤종용 Process for Preparing Oligopeptidenucleotide Probe Using Polymeric Photoacid Generator
WO2001013957A2 (en) 1999-08-24 2001-03-01 Cellgate, Inc. Enhancing drug delivery across and into epithelial tissues using oligo arginine moieties
AU6629200A (en) 1999-08-25 2001-03-19 Philip P Garner Alpha-helical peptide nucleic acid alpha-pna
US20030104622A1 (en) 1999-09-01 2003-06-05 Robbins Paul D. Identification of peptides that facilitate uptake and cytoplasmic and/or nuclear transport of proteins, DNA and viruses
WO2001018234A1 (en) 1999-09-03 2001-03-15 Yale University Method to identify gene function using small molecule probes
EP1242456B1 (en) 1999-11-18 2008-10-15 Oxford Biomedica (UK) Limited Scfv antibodies against disease associated molecules
US6451602B1 (en) 2000-03-02 2002-09-17 Isis Pharmaceuticals, Inc. Antisense modulation of PARP expression
US7279304B2 (en) 2000-12-18 2007-10-09 President And Fellows Of Harvard College Methods for preparation of macrocyclic molecules and macrocyclic molecules prepared thereby
US6451538B1 (en) 2000-12-22 2002-09-17 Isis Pharmaceuticals, Inc. Antisense modulation of CHK2 expression
US6455307B1 (en) 2001-02-08 2002-09-24 Isis Pharmaceuticals, Inc. Antisense modulation of casein kinase 2-alpha prime expression
US6455308B1 (en) 2001-08-01 2002-09-24 Isis Pharmaceuticals, Inc. Antisense modulation of serum amyloid A4 expression
WO2003052082A2 (en) 2001-12-18 2003-06-26 Whitehead Institute For Biomedical Research Fusion partner cells and uses thereof
ES2545090T3 (en) 2001-12-21 2015-09-08 Human Genome Sciences, Inc. Albumin and GCSF fusion proteins
US7611700B2 (en) 2002-09-09 2009-11-03 Hanall Pharmaceuticals, Co., Ltd. Protease resistant modified interferon alpha polypeptides
DE10335584B4 (en) 2003-07-31 2006-06-29 Philipps-Universität Marburg Process for the preparation of cyclic molecules
WO2005086654A2 (en) 2004-02-27 2005-09-22 The Trustees Of Princeton University Self-cleaving affinity tags and methods of use
JP2008507298A (en) 2004-05-19 2008-03-13 マキシジェン， インコーポレイテッド Interferon alpha polypeptides and conjugates
US7524831B2 (en) 2005-03-02 2009-04-28 Schering Corporation Treatments for Flaviviridae virus infection
US7625564B2 (en) 2006-01-27 2009-12-01 Novagen Holding Corporation Recombinant human EPO-Fc fusion proteins with prolonged half-life and enhanced erythropoietic activity in vivo
US8940501B2 (en) * 2009-01-30 2015-01-27 Whitehead Institute For Biomedical Research Methods for ligation and uses thereof
US20130122043A1 (en) 2010-04-20 2013-05-16 Whitehead Institute For Biomedical Research Modified polypeptides and proteins and uses thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030022178A1 (en) * 1999-04-15 2003-01-30 Olaf Schneewind Identification of sortase gene
WO2005051976A2 (en) * 2003-11-20 2005-06-09 Ansata Therapeutics, Inc. Protein and peptide ligation processes and one-step purification processes
US20090088372A1 (en) * 2006-03-22 2009-04-02 National Institute Of Immunology Novel Bioconjugates As Therapeutic Agent and Synthesis Thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ANTOS, J. M. ET AL.: "Lipid modification of proteins through sortase-catalyze d transpeptidation", JOURNAL OF AMERICAN CHEMICAL SOCIETY, vol. 130, no. 48, 3 September 2008 (2008-09-03), pages 16338 - 16343, XP008152225 *
See also references of EP2391714A4 *
TON-THAT, H. ET AL.: "Anchoring of surface proteins to the cell wall of Staph ylococcus aureus. Sortase catalyzed in vitro transpeptidation reaction using LPXTG peptide and NH(2)-Gly(3) substrate", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 13, 31 March 2000 (2000-03-31), pages 9876 - 9881, XP002288784 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105555794A (en) * 2013-07-10 2016-05-04 哈佛学院院长及董事 Compositions and methods relating to nucleic acid-protein complexes
CN107001446A (en) * 2014-12-17 2017-08-01 豪夫迈·罗氏有限公司 Using the double conjugation of polypeptides enzymatic one pot reactions of the single stage of sorting enzyme

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US8940501B2 (en) 2015-01-27
EP2391714A2 (en) 2011-12-07
EP2391714A4 (en) 2013-01-02
US20110321183A1 (en) 2011-12-29
WO2010087994A2 (en) 2010-08-05
EP2391714B2 (en) 2019-07-24
EP2391714B1 (en) 2017-04-05

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