WO2010040648A3 - Functional layers of biomolecules and living cells, and a novel system to produce such - Google Patents

Functional layers of biomolecules and living cells, and a novel system to produce such Download PDF

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Publication number
WO2010040648A3
WO2010040648A3 PCT/EP2009/062471 EP2009062471W WO2010040648A3 WO 2010040648 A3 WO2010040648 A3 WO 2010040648A3 EP 2009062471 W EP2009062471 W EP 2009062471W WO 2010040648 A3 WO2010040648 A3 WO 2010040648A3
Authority
WO
WIPO (PCT)
Prior art keywords
biomolecules
cells
examples
layer
produce
Prior art date
Application number
PCT/EP2009/062471
Other languages
French (fr)
Other versions
WO2010040648A2 (en
Inventor
Jan Fransaer
Malika Ammam
Original Assignee
Katholieke Universiteit Leuven, K.U.Leuven R&D
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0818310A external-priority patent/GB0818310D0/en
Priority claimed from GB0818332A external-priority patent/GB0818332D0/en
Priority claimed from GB0819715A external-priority patent/GB0819715D0/en
Priority claimed from GB0909379A external-priority patent/GB0909379D0/en
Application filed by Katholieke Universiteit Leuven, K.U.Leuven R&D filed Critical Katholieke Universiteit Leuven, K.U.Leuven R&D
Priority to EP09752309A priority Critical patent/EP2334845A2/en
Publication of WO2010040648A2 publication Critical patent/WO2010040648A2/en
Priority to US12/945,606 priority patent/US20110139617A1/en
Priority to AU2010241418A priority patent/AU2010241418A1/en
Publication of WO2010040648A3 publication Critical patent/WO2010040648A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
    • C—CHEMISTRY; METALLURGY
    • C25—ELECTROLYTIC OR ELECTROPHORETIC PROCESSES; APPARATUS THEREFOR
    • C25D—PROCESSES FOR THE ELECTROLYTIC OR ELECTROPHORETIC PRODUCTION OF COATINGS; ELECTROFORMING; APPARATUS THEREFOR
    • C25D13/00—Electrophoretic coating characterised by the process
    • C25D13/04—Electrophoretic coating characterised by the process with organic material
    • C—CHEMISTRY; METALLURGY
    • C25—ELECTROLYTIC OR ELECTROPHORETIC PROCESSES; APPARATUS THEREFOR
    • C25D—PROCESSES FOR THE ELECTROLYTIC OR ELECTROPHORETIC PRODUCTION OF COATINGS; ELECTROFORMING; APPARATUS THEREFOR
    • C25D13/00—Electrophoretic coating characterised by the process
    • C25D13/12—Electrophoretic coating characterised by the process characterised by the article coated
    • C—CHEMISTRY; METALLURGY
    • C25—ELECTROLYTIC OR ELECTROPHORETIC PROCESSES; APPARATUS THEREFOR
    • C25D—PROCESSES FOR THE ELECTROLYTIC OR ELECTROPHORETIC PRODUCTION OF COATINGS; ELECTROFORMING; APPARATUS THEREFOR
    • C25D13/00—Electrophoretic coating characterised by the process
    • C25D13/18—Electrophoretic coating characterised by the process using modulated, pulsed, or reversing current
    • C—CHEMISTRY; METALLURGY
    • C25—ELECTROLYTIC OR ELECTROPHORETIC PROCESSES; APPARATUS THEREFOR
    • C25D—PROCESSES FOR THE ELECTROLYTIC OR ELECTROPHORETIC PRODUCTION OF COATINGS; ELECTROFORMING; APPARATUS THEREFOR
    • C25D13/00—Electrophoretic coating characterised by the process
    • C25D13/22—Servicing or operating apparatus or multistep processes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5064—Endothelial cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes

Abstract

The present invention concerns a new process for depositing a thick compact layer of biomolecules for instance such a layer with thickness in the μm scale and, for depositing a thick compact layer of cells in the μm scale. The deposited layer is made by application of an unbalanced (asymmetrical) alternating voltage polarization between two electrodes to a dissolved biomolecule or cell from low conductivity solutions. The process allows the rapid manufacturing of sensors and the coating of devices with functional cells and biomolecules. Examples are provided on the preparation of functional sensors such as a glucose sensor is described in details, a hydrogen peroxide sensor and a glutamate sensor. Examples are also provided on the deposition of eukaryoric cells such as saccharomyces cerevisiae. The examples demonstrate a process that can be applied to coat devices with biomolecules and biological cells.
PCT/EP2009/062471 2008-10-06 2009-09-25 Functional layers of biomolecules and living cells, and a novel system to produce such WO2010040648A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP09752309A EP2334845A2 (en) 2008-10-06 2009-09-25 Functional layers of biomolecules and living cells, and a novel system to produce such
US12/945,606 US20110139617A1 (en) 2008-10-06 2010-11-12 Functional layers of biomolecules and living cells, and a novel system to produce such
AU2010241418A AU2010241418A1 (en) 2008-10-06 2010-11-12 Functional layers of biomolecules and living cells, and a novel system to produce such

Applications Claiming Priority (12)

Application Number Priority Date Filing Date Title
US19542708P 2008-10-06 2008-10-06
GB0818310A GB0818310D0 (en) 2008-10-06 2008-10-06 Functional layers of biomolecules and living cells, and a novel system to produce such
US61/195,427 2008-10-06
GB0818310.5 2008-10-06
US19555708P 2008-10-07 2008-10-07
GB0818332.9 2008-10-07
GB0818332A GB0818332D0 (en) 2008-10-07 2008-10-07 Functional layers of biomolecules and living cells, and a novel system to produce such
US61/195,557 2008-10-07
GB0819715A GB0819715D0 (en) 2008-10-28 2008-10-28 Functional layers of biomolecules and living cells, and a novel system to produce such
GB0819715.4 2008-10-28
GB0909379.0 2009-06-01
GB0909379A GB0909379D0 (en) 2009-06-01 2009-06-01 Functional layers of biomolecules and living cells, and a novel system to produce such

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/945,606 Continuation-In-Part US20110139617A1 (en) 2008-10-06 2010-11-12 Functional layers of biomolecules and living cells, and a novel system to produce such
AU2010241418A Division AU2010241418A1 (en) 2008-10-06 2010-11-12 Functional layers of biomolecules and living cells, and a novel system to produce such

Publications (2)

Publication Number Publication Date
WO2010040648A2 WO2010040648A2 (en) 2010-04-15
WO2010040648A3 true WO2010040648A3 (en) 2011-04-28

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2009/062471 WO2010040648A2 (en) 2008-10-06 2009-09-25 Functional layers of biomolecules and living cells, and a novel system to produce such

Country Status (3)

Country Link
US (1) US20110139617A1 (en)
EP (1) EP2334845A2 (en)
WO (1) WO2010040648A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5753720B2 (en) * 2010-04-22 2015-07-22 アークレイ株式会社 Biosensor
WO2012075331A2 (en) 2010-12-01 2012-06-07 Pinnacle Technology, Inc. Tissue implantable microbiosensor
CZ304417B6 (en) * 2012-03-30 2014-04-23 Mikrobiologický ústav AV ČR, v. v. i. Film of electrooxidized nostotrebin 6 with permselective properties for low-molecular biologically active substances, electrochemical sensor and method of determining these substances
US9717583B2 (en) 2014-03-13 2017-08-01 Cell and Molecular Tissue Engineering, LLC Sensors, cannulas, collars and coated surgical mesh, and corresponding systems and methods
US10405961B2 (en) 2013-03-14 2019-09-10 Cell and Molecular Tissue Engineering, LLC Coated surgical mesh, and corresponding systems and methods
US10130288B2 (en) 2013-03-14 2018-11-20 Cell and Molecular Tissue Engineering, LLC Coated sensors, and corresponding systems and methods
CA2907039C (en) 2013-03-15 2021-08-24 Brent A. Solina Method of producing an electroactive biofilm
EP2972268B1 (en) * 2013-03-15 2017-05-24 Roche Diabetes Care GmbH Methods of failsafing electrochemical measurements of an analyte as well as devices, apparatuses and systems incorporating the same
WO2014140177A2 (en) * 2013-03-15 2014-09-18 Roche Diagnostics Gmbh Methods of detecting high antioxidant levels during electrochemical measurements and failsafing an analyte concentration therefrom as well as devices, apparatuses and systems incorporting the same
EP3200858A4 (en) * 2014-09-30 2018-06-13 The Spectranetics Corporation Electrodeposition coating for medical devices
EP3589746B1 (en) * 2017-03-03 2021-05-19 Siemens Healthcare Diagnostics Inc. Nanobead containing biosensors and methods of production and use thereof
US11123068B2 (en) * 2019-11-08 2021-09-21 Covidien Lp Surgical staple cartridge

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3200058A (en) * 1961-08-23 1965-08-10 Ford Motor Co Cyclical current reversal for an electrophoretic deposition
DD215338A1 (en) * 1983-06-03 1984-11-07 Thuringia Sonneberg Veb PROCESS FOR ELECTROPHORETIC SEPARATION OF DISPERSED PARTICLES
US5166063A (en) * 1990-06-29 1992-11-24 Eli Lilly And Company Immobolization of biomolecules by enhanced electrophoretic precipitation
US5855753A (en) * 1996-11-26 1999-01-05 The Trustees Of Princeton University Method for electrohydrodynamically assembling patterned colloidal structures
US5872443A (en) * 1997-02-18 1999-02-16 Williamson; Floyd L. Electronic method for controlling charged particles to obtain optimum electrokinetic behavior
EP1132500A2 (en) * 2000-03-08 2001-09-12 Applied Materials, Inc. Method for electrochemical deposition of metal using modulated waveforms
WO2002033150A2 (en) * 2000-10-18 2002-04-25 Tecnu, Inc. Electrochemical processing power device
US6814845B2 (en) * 2001-11-21 2004-11-09 University Of Kansas Method for depositing an enzyme on an electrically conductive substrate
US20060065531A1 (en) * 2004-09-23 2006-03-30 Nanogen, Inc Methods and materials for optimization of electronic transportation and hybridization reactions

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1589327A (en) * 1925-04-07 1926-06-15 Eastman Kodak Co Electrodeposition of coatings of cellulosic compounds
GB1086325A (en) * 1963-07-26 1967-10-11 Goodlass Wall & Co Ltd Process of electrophoretic deposition by means of alternating current
JPS5117968B2 (en) * 1971-09-14 1976-06-07
US3959088A (en) * 1975-03-19 1976-05-25 The United States Of America As Represented By The Secretary Of The Army Method and apparatus for generating high amperage pulses from an A-C power source
JPS5569298A (en) * 1978-11-17 1980-05-24 Kureha Chem Ind Co Ltd Electrodeposition method of protein
US5126024A (en) * 1989-12-19 1992-06-30 Colorado School Of Mines Apparatus and method for concentrating microorganisms from a liquid medium on an electrode by electrodeposition
US5145684A (en) * 1991-01-25 1992-09-08 Sterling Drug Inc. Surface modified drug nanoparticles
DE4440337A1 (en) * 1994-11-11 1996-05-15 Dds Drug Delivery Services Ges Pharmaceutical nanosuspensions for drug application as systems with increased saturation solubility and dissolution rate
US5503723A (en) * 1995-02-08 1996-04-02 Eastman Kodak Company Isolation of ultra small particles
IL128632A (en) * 1996-08-22 2003-03-12 Rtp Pharma Corp Compositions comprising microparticles of water-insoluble substances and method for preparing same
US6045829A (en) * 1997-02-13 2000-04-04 Elan Pharma International Limited Nanocrystalline formulations of human immunodeficiency virus (HIV) protease inhibitors using cellulosic surface stabilizers
KR19990001564A (en) * 1997-06-16 1999-01-15 유충식 Azole antifungal agents with improved solubility and preparations containing them
US6241863B1 (en) * 1998-04-27 2001-06-05 Harold G. Monbouquette Amperometric biosensors based on redox enzymes
ATE307808T1 (en) * 1999-03-24 2005-11-15 Scherer Technologies Inc R P PHARMACEUTICAL FORMULATIONS WITH IMPROVED SOLUBILITY IN WATER
US6879143B2 (en) * 2002-04-16 2005-04-12 Motorola, Inc. Method of selectively aligning and positioning nanometer-scale components using AC fields
GB0319797D0 (en) * 2003-08-26 2003-09-24 Leuven K U Res & Dev Particle size reduction of poorly soluble drugs
US20080142366A1 (en) * 2006-12-13 2008-06-19 Prabhakar Apparao Tamirisa Incorporation of biomolecules in thin films

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3200058A (en) * 1961-08-23 1965-08-10 Ford Motor Co Cyclical current reversal for an electrophoretic deposition
DD215338A1 (en) * 1983-06-03 1984-11-07 Thuringia Sonneberg Veb PROCESS FOR ELECTROPHORETIC SEPARATION OF DISPERSED PARTICLES
US5166063A (en) * 1990-06-29 1992-11-24 Eli Lilly And Company Immobolization of biomolecules by enhanced electrophoretic precipitation
US5855753A (en) * 1996-11-26 1999-01-05 The Trustees Of Princeton University Method for electrohydrodynamically assembling patterned colloidal structures
US5872443A (en) * 1997-02-18 1999-02-16 Williamson; Floyd L. Electronic method for controlling charged particles to obtain optimum electrokinetic behavior
EP1132500A2 (en) * 2000-03-08 2001-09-12 Applied Materials, Inc. Method for electrochemical deposition of metal using modulated waveforms
WO2002033150A2 (en) * 2000-10-18 2002-04-25 Tecnu, Inc. Electrochemical processing power device
US6814845B2 (en) * 2001-11-21 2004-11-09 University Of Kansas Method for depositing an enzyme on an electrically conductive substrate
US20060065531A1 (en) * 2004-09-23 2006-03-30 Nanogen, Inc Methods and materials for optimization of electronic transportation and hybridization reactions

Also Published As

Publication number Publication date
EP2334845A2 (en) 2011-06-22
WO2010040648A2 (en) 2010-04-15
US20110139617A1 (en) 2011-06-16

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