WO2010027812A3 - Microfluidic systems incorporating flow-through membranes - Google Patents

Microfluidic systems incorporating flow-through membranes Download PDF

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Publication number
WO2010027812A3
WO2010027812A3 PCT/US2009/054941 US2009054941W WO2010027812A3 WO 2010027812 A3 WO2010027812 A3 WO 2010027812A3 US 2009054941 W US2009054941 W US 2009054941W WO 2010027812 A3 WO2010027812 A3 WO 2010027812A3
Authority
WO
WIPO (PCT)
Prior art keywords
membrane
assay
air
sample
target
Prior art date
Application number
PCT/US2009/054941
Other languages
French (fr)
Other versions
WO2010027812A2 (en
Inventor
Dean Y. Stevens
Lisa K. Lafleur
Barry R. Lutz
Paolo Spicar-Mihalic
Paul Yager
Original Assignee
University Of Washington
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Of Washington filed Critical University Of Washington
Priority to US13/060,664 priority Critical patent/US20110151479A1/en
Publication of WO2010027812A2 publication Critical patent/WO2010027812A2/en
Publication of WO2010027812A3 publication Critical patent/WO2010027812A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation

Abstract

Disclosed is a flow-through membrane assay that takes advantage of a high surface area and rapid transport while allowing individual control over flow rates and times for each step of a multi-step assay. A microfluidic card features channels in communication with a porous membrane, channels on either side of membrane to allow transverse flow across the membrane, capturing a labeled target from the sample by flowing the sample across the membrane, or capturing a target from the sample followed by flowing a reagent containing a label that binds to the target. Fluid can be pushed or pulled through the assay membrane by external control. Air near the membrane is managed by diverting air between fluids to a channel upstream of the assay membrane, venting air between fluids through a hydrophobic membrane upstream of the assay membrane, and/or by venting trapped air through a hydrophobic membrane downstream of the assay membrane.
PCT/US2009/054941 2008-08-25 2009-08-25 Microfluidic systems incorporating flow-through membranes WO2010027812A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US13/060,664 US20110151479A1 (en) 2008-08-25 2009-08-25 Microfluidic systems incorporating flow-through membranes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9163908P 2008-08-25 2008-08-25
US61/091,639 2008-08-25

Publications (2)

Publication Number Publication Date
WO2010027812A2 WO2010027812A2 (en) 2010-03-11
WO2010027812A3 true WO2010027812A3 (en) 2010-05-20

Family

ID=41797785

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/054941 WO2010027812A2 (en) 2008-08-25 2009-08-25 Microfluidic systems incorporating flow-through membranes

Country Status (2)

Country Link
US (1) US20110151479A1 (en)
WO (1) WO2010027812A2 (en)

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US9759718B2 (en) 2009-11-23 2017-09-12 Cyvek, Inc. PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use
US10065403B2 (en) 2009-11-23 2018-09-04 Cyvek, Inc. Microfluidic assay assemblies and methods of manufacture
US9500645B2 (en) 2009-11-23 2016-11-22 Cyvek, Inc. Micro-tube particles for microfluidic assays and methods of manufacture
WO2011063408A1 (en) 2009-11-23 2011-05-26 Cyvek, Inc. Method and apparatus for performing assays
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US8628972B2 (en) * 2011-01-11 2014-01-14 The Regents Of The University Of California Microfluidic devices and methods for malaria detection
FR2972117B1 (en) * 2011-03-04 2013-12-20 Centre Nat Rech Scient MICROFLUIDIC SYSTEM FOR CONTROLLING A PROFILE OF CONCENTRATION OF MOLECULES LIKELY TO STIMULATE A TARGET
EP2689253B1 (en) 2011-03-22 2021-03-10 Cyvek, Inc. Microfluidic devices and methods of manufacture
CA2856063A1 (en) 2011-11-23 2013-05-30 The Governing Council Of The University Of Toronto Devices and methods for producing planar polymeric materials using microfluidics
KR102070469B1 (en) 2012-03-08 2020-01-28 싸이벡, 아이엔씨 Microfluidic assay assemblies and methods of manufacture
EP2879789B1 (en) * 2012-07-30 2019-09-04 NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen System comprising a connecting plate for a microfluidic chip, the microfluidic chip and a control unit
WO2014039514A2 (en) * 2012-09-05 2014-03-13 President And Fellows Of Harvard College Removing bubbles in microfluidic systems
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US10518262B2 (en) 2012-12-21 2019-12-31 Perkinelmer Health Sciences, Inc. Low elasticity films for microfluidic use
WO2014100732A1 (en) * 2012-12-21 2014-06-26 Micronics, Inc. Fluidic circuits and related manufacturing methods
US10386377B2 (en) 2013-05-07 2019-08-20 Micronics, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching
CN105189750B (en) 2013-05-07 2020-07-28 珀金埃尔默健康科学有限公司 Method for preparing nucleic acid-containing sample using clay mineral and alkaline solution
JP6484222B2 (en) 2013-05-07 2019-03-13 マイクロニクス， インコーポレイテッド Devices for nucleic acid preparation and analysis
EP3822635A1 (en) 2014-03-07 2021-05-19 The Regents Of The University Of California Devices for integrating analyte extraction, concentration and detection
WO2015188171A1 (en) * 2014-06-06 2015-12-10 Berkeley Lights, Inc. Isolating microfluidic structures and trapping bubbles
US9702793B2 (en) 2015-03-16 2017-07-11 Todd A Balisky Variable volume sample capture device
CN108139402B (en) 2015-09-04 2022-10-18 加利福尼亚大学董事会 Methods and devices for analyte collection, extraction, concentration and detection for clinical applications
US10228367B2 (en) 2015-12-01 2019-03-12 ProteinSimple Segmented multi-use automated assay cartridge
AU2017277631B2 (en) 2016-06-09 2024-01-25 The Regents Of The University Of California Biomarker concentration and signal amplification for use in paper-based immunoassays and a single platform for extracting, concentrating, and amplifying DNA
WO2018039139A1 (en) 2016-08-22 2018-03-01 The Regents Of The University Of California Hydrogel platform for aqueous two-phase concentration of a target to enhance its detection
JP6918490B2 (en) * 2016-12-28 2021-08-11 シスメックス株式会社 Analytical method and analyzer
WO2018183211A1 (en) 2017-03-27 2018-10-04 The Regents Of The University Of California Semi-quantitative lateral-flow immunoassay for the detection of csf leaks
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US20230372933A1 (en) * 2020-10-08 2023-11-23 Lmx Medtech Llc Method for Sample Collection and Metering

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Also Published As

Publication number Publication date
US20110151479A1 (en) 2011-06-23
WO2010027812A2 (en) 2010-03-11

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