WO2009133466A3 - Asymmetric adapter library construction - Google Patents

Asymmetric adapter library construction Download PDF

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Publication number
WO2009133466A3
WO2009133466A3 PCT/IB2009/005759 IB2009005759W WO2009133466A3 WO 2009133466 A3 WO2009133466 A3 WO 2009133466A3 IB 2009005759 W IB2009005759 W IB 2009005759W WO 2009133466 A3 WO2009133466 A3 WO 2009133466A3
Authority
WO
WIPO (PCT)
Prior art keywords
library construction
asymmetric adapter
adapter library
asymmetric
compositions
Prior art date
Application number
PCT/IB2009/005759
Other languages
French (fr)
Other versions
WO2009133466A2 (en
Inventor
Gi Mikawa
Original Assignee
Population Genetics Technologies Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Population Genetics Technologies Ltd. filed Critical Population Genetics Technologies Ltd.
Publication of WO2009133466A2 publication Critical patent/WO2009133466A2/en
Publication of WO2009133466A3 publication Critical patent/WO2009133466A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor

Abstract

The present invention provides methods and compositions for asymmetrically tagging a nucleic acid fragment using asymmetric adapters.
PCT/IB2009/005759 2008-04-30 2009-04-29 Asymmetric adapter library construction WO2009133466A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4932308P 2008-04-30 2008-04-30
US61/049,323 2008-04-30

Publications (2)

Publication Number Publication Date
WO2009133466A2 WO2009133466A2 (en) 2009-11-05
WO2009133466A3 true WO2009133466A3 (en) 2010-10-07

Family

ID=41255488

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2009/005759 WO2009133466A2 (en) 2008-04-30 2009-04-29 Asymmetric adapter library construction

Country Status (2)

Country Link
US (4) US8029993B2 (en)
WO (1) WO2009133466A2 (en)

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CN106459968B (en) * 2014-01-31 2020-02-21 斯威夫特生物科学股份有限公司 Improved method for processing DNA substrates

Also Published As

Publication number Publication date
US8883990B2 (en) 2014-11-11
WO2009133466A2 (en) 2009-11-05
US8029993B2 (en) 2011-10-04
US20140030704A1 (en) 2014-01-30
US20090275087A1 (en) 2009-11-05
US20130029380A1 (en) 2013-01-31
US20120083017A1 (en) 2012-04-05
US8420319B2 (en) 2013-04-16
US8288097B2 (en) 2012-10-16

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