WO2009102896A3 - Isothermal nucleic acid amplification methods and compositions - Google Patents

Isothermal nucleic acid amplification methods and compositions Download PDF

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Publication number
WO2009102896A3
WO2009102896A3 PCT/US2009/033964 US2009033964W WO2009102896A3 WO 2009102896 A3 WO2009102896 A3 WO 2009102896A3 US 2009033964 W US2009033964 W US 2009033964W WO 2009102896 A3 WO2009102896 A3 WO 2009102896A3
Authority
WO
WIPO (PCT)
Prior art keywords
compositions
nucleic acid
target
acid amplification
amplification methods
Prior art date
Application number
PCT/US2009/033964
Other languages
French (fr)
Other versions
WO2009102896A2 (en
Inventor
Nurith Kurn
Shenglong Wang
Original Assignee
Nugen Technologies, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nugen Technologies, Inc. filed Critical Nugen Technologies, Inc.
Priority to EP09709858A priority Critical patent/EP2250283A2/en
Publication of WO2009102896A2 publication Critical patent/WO2009102896A2/en
Publication of WO2009102896A3 publication Critical patent/WO2009102896A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Abstract

Methods and compositions are provided related to the amplification of target polynucleotide sequences as well as total RNA and total DNA amplification. In some embodiments, the methods and compositions also allow for the immobilization and capture of target polynucleotides with defined 3' and or 5' sequences to solid surfaces. The polynucleotides attached to the solid surfaces can be amplified or eluted for downstream processing. In some cases, nucleotides attached to solid surfaces can be used for high throughput sequencing of nucleotide sequences related to target DNA or target RNA.
PCT/US2009/033964 2008-02-12 2009-02-12 Isothermal nucleic acid amplification methods and compositions WO2009102896A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP09709858A EP2250283A2 (en) 2008-02-12 2009-02-12 Isothermal nucleic acid amplification methods and compositions

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US2814608P 2008-02-12 2008-02-12
US61/028,146 2008-02-12
US3869608P 2008-03-21 2008-03-21
US61/038,696 2008-03-21
US7499108P 2008-06-23 2008-06-23
US61/074,991 2008-06-23
US8581108P 2008-08-01 2008-08-01
US61/085,811 2008-08-01

Publications (2)

Publication Number Publication Date
WO2009102896A2 WO2009102896A2 (en) 2009-08-20
WO2009102896A3 true WO2009102896A3 (en) 2009-11-19

Family

ID=40957495

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2009/033936 WO2009102878A2 (en) 2008-02-12 2009-02-12 Method for archiving and clonal expansion
PCT/US2009/033964 WO2009102896A2 (en) 2008-02-12 2009-02-12 Isothermal nucleic acid amplification methods and compositions

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2009/033936 WO2009102878A2 (en) 2008-02-12 2009-02-12 Method for archiving and clonal expansion

Country Status (2)

Country Link
EP (2) EP2247727A4 (en)
WO (2) WO2009102878A2 (en)

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CN102439177B (en) 2009-04-02 2014-10-01 弗卢伊蒂格姆公司 Multi-primer amplification method for barcoding of target nucleic acids
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9309566B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
EP2369325A1 (en) 2010-03-12 2011-09-28 Eppendorf Ag Array analysis for online detection
KR101726238B1 (en) * 2010-10-15 2017-04-12 (주) 하임바이오텍 Method for non-specifically extending base sequences of target polynucleotide, primer composition for performing the method and kit for improving sensitivity of detecting target polynucleotide
TWI435935B (en) * 2010-12-06 2014-05-01 Univ Nat Cheng Kung Kit and method for rapidly detecting a target nucleic acid fragment
EP2652148B1 (en) * 2010-12-17 2016-11-30 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
SG10201605049QA (en) 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
WO2013112923A1 (en) 2012-01-26 2013-08-01 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
JP6445426B2 (en) 2012-05-10 2018-12-26 ザ ジェネラル ホスピタル コーポレイション Method for determining nucleotide sequence
CA2877094A1 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
EP3068883B1 (en) 2013-11-13 2020-04-29 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
WO2015112974A1 (en) 2014-01-27 2015-07-30 The General Hospital Corporation Methods of preparing nucleic acids for sequencing
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
GB201410646D0 (en) * 2014-06-14 2014-07-30 Illumina Cambridge Ltd Methods of increasing sequencing accuracy
SG11201700891SA (en) 2014-08-06 2017-03-30 Nugen Technologies Inc Digital measurements from targeted sequencing
WO2016149837A1 (en) * 2015-03-25 2016-09-29 Axela Inc. Solid phase nucleic acid target capture and replication using strand displacing polymerases
EP3390658B1 (en) 2015-12-16 2022-08-03 Standard BioTools Inc. High-level multiplex amplification
CN105624302B (en) * 2016-02-04 2019-10-18 浙江大学 Composite label and its application for arthropod bio-diversity high-flux sequence
AU2017328950B2 (en) 2016-09-15 2023-09-14 Archerdx, Llc Methods of nucleic acid sample preparation
CA3037190A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation for analysis of cell-free dna
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
EP3927717A4 (en) * 2019-02-19 2022-12-21 Pioneer Biolabs, LLC Guide strand library construction and methods of use thereof
CN111662962B (en) * 2020-06-09 2023-06-23 珠海市坤元科技有限公司 Nucleic acid isothermal amplification method adopting bidirectional strand replacement loop circulation

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Publication number Priority date Publication date Assignee Title
US6692918B2 (en) * 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
US6946251B2 (en) * 2001-03-09 2005-09-20 Nugen Technologies, Inc. Methods and compositions for amplification of RNA sequences using RNA-DNA composite primers
WO2007030759A2 (en) * 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure

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AU2004254552B2 (en) * 2003-01-29 2008-04-24 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
JP2009506788A (en) * 2005-09-06 2009-02-19 ジェン−プローブ・インコーポレーテッド Methods, compositions and kits for isothermal amplification of nucleic acids

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6692918B2 (en) * 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
US6946251B2 (en) * 2001-03-09 2005-09-20 Nugen Technologies, Inc. Methods and compositions for amplification of RNA sequences using RNA-DNA composite primers
WO2007030759A2 (en) * 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KURN, N. ET AL.: "Novel isothermal, linear nucleic acid amplification systems for highly multiplexed applications.", CLIN. CHEM., vol. 51, no. 10, 25 August 2005 (2005-08-25), pages 1973 - 1981, XP002420278 *

Also Published As

Publication number Publication date
EP2250283A2 (en) 2010-11-17
WO2009102896A2 (en) 2009-08-20
WO2009102878A2 (en) 2009-08-20
EP2247727A4 (en) 2011-08-03
WO2009102878A3 (en) 2010-01-21
EP2247727A2 (en) 2010-11-10

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