WO2009052209A3 - Isolation of stem cells and effective control of contamination - Google Patents

Isolation of stem cells and effective control of contamination Download PDF

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Publication number
WO2009052209A3
WO2009052209A3 PCT/US2008/080039 US2008080039W WO2009052209A3 WO 2009052209 A3 WO2009052209 A3 WO 2009052209A3 US 2008080039 W US2008080039 W US 2008080039W WO 2009052209 A3 WO2009052209 A3 WO 2009052209A3
Authority
WO
WIPO (PCT)
Prior art keywords
stem cells
isolation
contamination
effective control
relates
Prior art date
Application number
PCT/US2008/080039
Other languages
French (fr)
Other versions
WO2009052209A2 (en
Inventor
Kathy E Mitchell
Steven M Hoynowski
Original Assignee
Univ Kansas
Kathy E Mitchell
Steven M Hoynowski
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Kansas, Kathy E Mitchell, Steven M Hoynowski filed Critical Univ Kansas
Publication of WO2009052209A2 publication Critical patent/WO2009052209A2/en
Publication of WO2009052209A3 publication Critical patent/WO2009052209A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/135—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones

Abstract

The invention relates to the improved isolation and culture of stem cells. More particularly the invention relates to a method that provides for increased recovery of sterile and viable stem cells from umbilical cord and other tissue sources where stem cells are not readily dissociated.
PCT/US2008/080039 2007-10-16 2008-10-15 Isolation of stem cells and effective control of contamination WO2009052209A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US98025207P 2007-10-16 2007-10-16
US60/980,252 2007-10-16

Publications (2)

Publication Number Publication Date
WO2009052209A2 WO2009052209A2 (en) 2009-04-23
WO2009052209A3 true WO2009052209A3 (en) 2009-08-13

Family

ID=40568060

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/080039 WO2009052209A2 (en) 2007-10-16 2008-10-15 Isolation of stem cells and effective control of contamination

Country Status (1)

Country Link
WO (1) WO2009052209A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040248290A1 (en) * 2002-05-31 2004-12-09 Marco Riedel Culture systems for the sterile continuous cultivation of cells
US20050058631A1 (en) * 2003-06-27 2005-03-17 Kihm Anthony J. Postpartum cells derived from placental tissue, and methods of making and using the same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040248290A1 (en) * 2002-05-31 2004-12-09 Marco Riedel Culture systems for the sterile continuous cultivation of cells
US20050058631A1 (en) * 2003-06-27 2005-03-17 Kihm Anthony J. Postpartum cells derived from placental tissue, and methods of making and using the same

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WEISS, M.L. ET AL.: "'Human Umbilibal Cord Matrix Stem Cells: Preliminary Characterization and Effect of Transplantation in a Rodent Model of Parkinson's Disease.'", STEM CELLS, vol. 24, 2006, pages 781 - 792 *

Also Published As

Publication number Publication date
WO2009052209A2 (en) 2009-04-23

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