WO2009042291A3 - Accelerated cascade amplification (aca) of nucleic acids comprising strand and sequence specific dna nicking - Google Patents
Accelerated cascade amplification (aca) of nucleic acids comprising strand and sequence specific dna nicking Download PDFInfo
- Publication number
- WO2009042291A3 WO2009042291A3 PCT/US2008/072145 US2008072145W WO2009042291A3 WO 2009042291 A3 WO2009042291 A3 WO 2009042291A3 US 2008072145 W US2008072145 W US 2008072145W WO 2009042291 A3 WO2009042291 A3 WO 2009042291A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- primer
- amplification
- internal
- strand
- primers
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Abstract
Particular aspects provide nucleic acid amplification and detection methods comprising: providing a reaction mixture containing a target nucleic acid with an amplifiable target sequence, forward and reverse external nick-directing primers (ND-primers), at least one internal ND-primer, a strand-displacing DNA polymerase, a nick-directing endonuclease for strand-specific cleavage of ND-primer-extension products, and deoxynucleoside 5 -triphosphates; and incubating the reaction mixture with reagents, and under conditions suitable to provide for amplification of the amplifiable target sequence, wherein the amplification comprises primer extension, by least one internal ND-primer, of an external ND-primer extension product comprising the amplifiable target sequence or a portion thereof but lacking the respective external ND-primer sequence or a portion thereof. Preferably, amplification comprises using a plurality of internal ND-primers, extension of one internal ND-primer extension product by a different internal ND-primer, and amplification is isothermal and synergistic with respect to the number of primers employed. Amplification and detection kits are provided.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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US95393107P | 2007-08-03 | 2007-08-03 | |
US60/953,931 | 2007-08-03 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009042291A2 WO2009042291A2 (en) | 2009-04-02 |
WO2009042291A3 true WO2009042291A3 (en) | 2009-12-30 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2008/072145 WO2009042291A2 (en) | 2007-08-03 | 2008-08-04 | Accelerated cascade amplification (aca) of nucleic acids comprising strand and sequence specific dna nicking |
Country Status (2)
Country | Link |
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US (1) | US8143006B2 (en) |
WO (1) | WO2009042291A2 (en) |
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WO2011037802A2 (en) | 2009-09-28 | 2011-03-31 | Igor Kutyavin | Methods and compositions for detection of nucleic acids based on stabilized oligonucleotide probe complexes |
WO2012044956A1 (en) * | 2010-10-01 | 2012-04-05 | Ibis Biosciences, Inc. | Targeted genome amplification methods |
US9523120B2 (en) | 2011-06-14 | 2016-12-20 | University of Pittsburgh—of the Commonwealth System of Higher Education | Method of amplifying a nucleic acid |
US9777319B2 (en) | 2012-06-29 | 2017-10-03 | General Electric Company | Method for isothermal DNA amplification starting from an RNA template |
US10590474B2 (en) | 2013-03-11 | 2020-03-17 | Elitechgroup B.V. | Methods for true isothermal strand displacement amplification |
US20140255928A1 (en) * | 2013-03-11 | 2014-09-11 | Elitech Holding B.V. | Methods for true isothermal strand displacement amplification |
GB201611469D0 (en) | 2016-06-30 | 2016-08-17 | Lumiradx Tech Ltd | Improvements in or relating to nucleic acid amplification processes |
US10724017B1 (en) | 2017-12-06 | 2020-07-28 | Cepheid | Inhibition of DNA polymerases by uracil-DNA glycosylase-cleavable oligonucleotide ligands |
US10689629B1 (en) | 2017-12-06 | 2020-06-23 | Cepheid | Inhibition of nucleic acid polymerases by endonuclease V-cleavable circular oligonucleotide ligands |
US10724083B1 (en) | 2017-12-06 | 2020-07-28 | Cepheid | Inhibition of nucleic acid polymerases by endonuclease V-cleavable oligonucleotide ligands |
GB2569965A (en) | 2018-01-04 | 2019-07-10 | Lumiradx Uk Ltd | Improvements in or relating to amplification of nucleic acids |
US20220002792A1 (en) * | 2018-10-29 | 2022-01-06 | Igor V. Kutyavin | Accelerated polymerase chain reaction |
CN111893214B (en) * | 2020-07-08 | 2023-03-28 | 重庆医科大学 | Double-template multi-cycle G-triplet machine and application thereof in HIV detection |
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Also Published As
Publication number | Publication date |
---|---|
US20090047678A1 (en) | 2009-02-19 |
US8143006B2 (en) | 2012-03-27 |
WO2009042291A2 (en) | 2009-04-02 |
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