WO2009039170A3 - Integrated protein chip assay - Google Patents
Integrated protein chip assay Download PDFInfo
- Publication number
- WO2009039170A3 WO2009039170A3 PCT/US2008/076667 US2008076667W WO2009039170A3 WO 2009039170 A3 WO2009039170 A3 WO 2009039170A3 US 2008076667 W US2008076667 W US 2008076667W WO 2009039170 A3 WO2009039170 A3 WO 2009039170A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- methods
- replicates
- scattered
- sensitivity
- relates
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
- C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
Abstract
The present invention relates to novel methodologies for performing multiplexed assays with high precision and sensitivity. In particular, the present invention relates to improving assay sensitivity and precision by combining the normalization of multiplexed assay data using an internal standard with scattered application of samples and standards replicates throughout sample wells on a slide or set of slides as well as scattered replicates of arrayed probes in a single well. These compositions and methods can be used to perform multiplexed assays for analytes in patient and other test samples. In particular, these methods have applications for Quantitative Multi-analyte Immunoassays (QMI) to measure proteins in human serum and plasma.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US97292807P | 2007-09-17 | 2007-09-17 | |
US60/972,928 | 2007-09-17 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009039170A2 WO2009039170A2 (en) | 2009-03-26 |
WO2009039170A3 true WO2009039170A3 (en) | 2009-05-07 |
Family
ID=40455119
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2008/076667 WO2009039170A2 (en) | 2007-09-17 | 2008-09-17 | Integrated protein chip assay |
Country Status (2)
Country | Link |
---|---|
US (1) | US20090075828A1 (en) |
WO (1) | WO2009039170A2 (en) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9404923B2 (en) | 2012-02-07 | 2016-08-02 | Intuitive Biosciences, Inc. | Mycobacterium tuberculosis specific peptides for detection of infection or immunization in non-human primates |
CN110187122A (en) * | 2012-09-05 | 2019-08-30 | 亚利桑那州评议委员会,亚利桑那州法人团体,代理和代表亚利桑那州立大学 | It was found that therapeutic target calibration method |
WO2015060511A1 (en) * | 2013-10-22 | 2015-04-30 | 주식회사 바이오이즈 | Marker for generating binding information on biomolecules and nucleic acids, preparation method therefor, and method and apparatus for analyzing biomolecule by using same |
KR101829668B1 (en) | 2013-03-27 | 2018-02-14 | 김성천 | Nucleic acid chip and Reference Substance for Obtaining Bind information of sigle-stranded nucleic acid and Biomolecule, Manufacturing Method Thereof, and Method and apparatus for analyzing Biomolecules Using Nucleic acid chip and Reference Substances |
WO2016014612A2 (en) | 2014-07-23 | 2016-01-28 | Inova Diagnostics, Inc. | Compositions and methods for the diagnosis of rheumatoid arthritis |
WO2016094459A2 (en) * | 2014-12-09 | 2016-06-16 | Berkeley Lights, Inc. | Automated detection and repositioning of micro-objects in microfluidic devices |
EP3230718B1 (en) | 2014-12-09 | 2022-03-02 | Berkeley Lights, Inc. | Automated detection of assay-positive areas or of analyte quantities in microfluidic devices |
US10758886B2 (en) | 2015-09-14 | 2020-09-01 | Arizona Board Of Regents On Behalf Of Arizona State University | Conditioned surfaces for in situ molecular array synthesis |
EP3387154A4 (en) | 2015-12-09 | 2019-05-15 | Intuitive Biosciences, Inc. | Automated silver enhancement system |
WO2020168168A1 (en) | 2019-02-15 | 2020-08-20 | Inova Diagnostics, Inc. | Compositions and methods for diagnosing and assessing rheumatoid arthritis |
AU2021273070A1 (en) | 2020-05-15 | 2022-11-03 | Inova Diagnostics, Inc. | Compositions and methods for diagnosing and assessing rheumatoid arthritis using protein-arginine deiminase 1 (PAD1) autoantigens |
CN117881963A (en) | 2021-07-15 | 2024-04-12 | 福斯分析仪器公司 | System for simultaneous detection of multiple analytes |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001009281A1 (en) * | 1999-08-02 | 2001-02-08 | Btf Pty Ltd | Internal quality control |
US20030093225A1 (en) * | 2001-11-13 | 2003-05-15 | Fathallah-Shaykh Hassan M. | Method for reducing noise in analytical assays |
WO2004018623A2 (en) * | 2002-08-16 | 2004-03-04 | Clinical Microarrays, Inc. | Substrates for isolating, reacting and microscopically analyzing materials |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FI76888C (en) * | 1981-04-29 | 1988-12-12 | Ciba Geigy Ag | New agents and packaging for immunological analysis. |
AU687973B2 (en) * | 1993-12-17 | 1998-03-05 | Hitco Carbon Composites, Inc. | Silicone composite materials having high temperature resistance |
DE60000583T3 (en) * | 1999-05-19 | 2009-04-30 | Eppendorf Array Technologies | METHOD FOR IDENTIFYING AND / OR QUANTIFYING A TARGET LINK |
US7063979B2 (en) * | 2001-06-13 | 2006-06-20 | Grace Bio Labs., Inc. | Interface between substrates having microarrays and microtiter plates |
US20030139504A1 (en) * | 2001-11-12 | 2003-07-24 | General Electric Company | Flame retardant resinous compositions and method |
US6861251B2 (en) * | 2003-02-24 | 2005-03-01 | Pritest, Inc. | Translucent solid matrix assay device for microarray analysis |
KR100557541B1 (en) * | 2003-06-30 | 2006-03-03 | 주식회사 하이닉스반도체 | Manufacturing method for semiconductor device |
TWI254035B (en) * | 2004-02-23 | 2006-05-01 | Agnitio Science & Technology C | A process for the preparation of a nitrocellulose thin film |
US20050214882A1 (en) * | 2004-03-25 | 2005-09-29 | Ez Bio Inc. | Reagents, methods and kits for the universal rapid immuno-detection |
US20080032420A1 (en) * | 2004-03-30 | 2008-02-07 | Lambert James L | Surface Enhanced Raman Scattering and Multiplexed Diagnostic Assays |
CA2563796A1 (en) * | 2004-04-23 | 2005-11-24 | The University Of Georgia Research Foundation, Inc. | Diagnostic assay for trypanosoma cruzi infection |
ATE555853T1 (en) * | 2004-05-21 | 2012-05-15 | Schott Ag | DEVICE IN THE FORM OF A MICROTITER PLATE FOR MULTIPLEXED ARRAY |
US20080108513A1 (en) * | 2006-06-01 | 2008-05-08 | Northwestern University | Cellular Arrays |
-
2008
- 2008-09-17 US US12/212,344 patent/US20090075828A1/en not_active Abandoned
- 2008-09-17 WO PCT/US2008/076667 patent/WO2009039170A2/en active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001009281A1 (en) * | 1999-08-02 | 2001-02-08 | Btf Pty Ltd | Internal quality control |
US20030093225A1 (en) * | 2001-11-13 | 2003-05-15 | Fathallah-Shaykh Hassan M. | Method for reducing noise in analytical assays |
WO2004018623A2 (en) * | 2002-08-16 | 2004-03-04 | Clinical Microarrays, Inc. | Substrates for isolating, reacting and microscopically analyzing materials |
Non-Patent Citations (1)
Title |
---|
PERLEE L. T. ET AL.: "Development and standardization of multiplexed antibody microarrays for use in quantitative proteomics.", PROTEOME SCIENCE., vol. 2, December 2004 (2004-12-01), pages 9 * |
Also Published As
Publication number | Publication date |
---|---|
US20090075828A1 (en) | 2009-03-19 |
WO2009039170A2 (en) | 2009-03-26 |
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