WO2009034514A3 - Dielectrophoretic device and method for cell membrane studies - Google Patents
Dielectrophoretic device and method for cell membrane studies Download PDFInfo
- Publication number
- WO2009034514A3 WO2009034514A3 PCT/IB2008/053622 IB2008053622W WO2009034514A3 WO 2009034514 A3 WO2009034514 A3 WO 2009034514A3 IB 2008053622 W IB2008053622 W IB 2008053622W WO 2009034514 A3 WO2009034514 A3 WO 2009034514A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- cell membrane
- cage
- particle
- particles
- detection system
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C5/00—Separating dispersed particles from liquids by electrostatic effect
- B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C5/00—Separating dispersed particles from liquids by electrostatic effect
- B03C5/02—Separators
- B03C5/022—Non-uniform field separators
- B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
Abstract
A dielectrophoretic device and method is described for manipulating one or more particles, e.g. living cells. The device comprises a plurality of electrodes in the form of a DEP cage, and a particle movement detection system for detecting movement of the one or more particles within the cage. Particle movement is indicative of passing through the cross-over frequency. The particle movement detection system includes a particle presence sensor and this can be located eccentrically with respect to a trapping point of the DEP cage. The device and method may be used for classification, identification, of quantification of diseased versus healthy cells, for diagnosis, for medical research and development and in therapy. They are particularly useful for investigating living cells whose cell membrane alters in conductivity or permeability.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP07116010.5 | 2007-09-10 | ||
EP07116010A EP2042239A1 (en) | 2007-09-10 | 2007-09-10 | Dielectrophoretic device and method for cell membrane studies |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009034514A2 WO2009034514A2 (en) | 2009-03-19 |
WO2009034514A3 true WO2009034514A3 (en) | 2009-10-22 |
Family
ID=39107761
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2008/053622 WO2009034514A2 (en) | 2007-09-10 | 2008-09-08 | Dielectrophoretic device and method for cell membrane studies |
Country Status (2)
Country | Link |
---|---|
EP (1) | EP2042239A1 (en) |
WO (1) | WO2009034514A2 (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102166506A (en) * | 2011-03-02 | 2011-08-31 | 厦门大学 | Method for confining alternating current electric field of charged particles in solution |
US9683253B2 (en) | 2013-03-19 | 2017-06-20 | Imec | Method and device for identifying cells |
US10518273B2 (en) * | 2014-04-09 | 2019-12-31 | Apocell, Inc. | System and method for determining dielectrophoresis crossover frequencies |
CN103985428B (en) * | 2014-05-15 | 2017-02-15 | 清华大学 | Molecule three-dimensional rotation operation device based on dielectrophoresis and control method of molecule three-dimensional rotation operation device based on dielectrophoresis |
JP6441838B2 (en) * | 2016-01-29 | 2018-12-19 | 株式会社Afiテクノロジー | Analysis device and separation device |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998004355A1 (en) * | 1996-07-26 | 1998-02-05 | Btg International Limited | Apparatus and method for testing particles using dielectrophoresis |
US6294063B1 (en) * | 1999-02-12 | 2001-09-25 | Board Of Regents, The University Of Texas System | Method and apparatus for programmable fluidic processing |
EP1145766A2 (en) * | 2000-04-13 | 2001-10-17 | Wako Pure Chemical Industries Ltd | Electrode construction for dielectrophoretic apparatus and separation by dielectrophoresis |
US20020036142A1 (en) * | 2000-06-14 | 2002-03-28 | Peter Gascoyne | Systems and methods for cell subpopulation analysis |
US20040178068A1 (en) * | 2002-12-20 | 2004-09-16 | The University Of Texas System | Methods and apparatus for electrosmear analysis |
US6801311B1 (en) * | 1999-01-26 | 2004-10-05 | Evotec Technologies Gmbh | Process and device for the detection of microscopically small objects |
WO2005031300A2 (en) * | 2003-06-27 | 2005-04-07 | Purdue Research Foundation | Device for detecting biological and chemical particles |
US20060219939A1 (en) * | 2004-12-03 | 2006-10-05 | Nano Science Diagnostic, Inc. | Method and apparatus for low quantity detection of bioparticles in small sample volumes |
WO2007010367A2 (en) * | 2005-07-19 | 2007-01-25 | Silicon Biosystems S.P.A. | Method and apparatus for the manipulation and/or the detection of particles |
-
2007
- 2007-09-10 EP EP07116010A patent/EP2042239A1/en not_active Ceased
-
2008
- 2008-09-08 WO PCT/IB2008/053622 patent/WO2009034514A2/en active Application Filing
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998004355A1 (en) * | 1996-07-26 | 1998-02-05 | Btg International Limited | Apparatus and method for testing particles using dielectrophoresis |
US6801311B1 (en) * | 1999-01-26 | 2004-10-05 | Evotec Technologies Gmbh | Process and device for the detection of microscopically small objects |
US6294063B1 (en) * | 1999-02-12 | 2001-09-25 | Board Of Regents, The University Of Texas System | Method and apparatus for programmable fluidic processing |
EP1145766A2 (en) * | 2000-04-13 | 2001-10-17 | Wako Pure Chemical Industries Ltd | Electrode construction for dielectrophoretic apparatus and separation by dielectrophoresis |
US20020036142A1 (en) * | 2000-06-14 | 2002-03-28 | Peter Gascoyne | Systems and methods for cell subpopulation analysis |
US20040178068A1 (en) * | 2002-12-20 | 2004-09-16 | The University Of Texas System | Methods and apparatus for electrosmear analysis |
WO2005031300A2 (en) * | 2003-06-27 | 2005-04-07 | Purdue Research Foundation | Device for detecting biological and chemical particles |
US20060219939A1 (en) * | 2004-12-03 | 2006-10-05 | Nano Science Diagnostic, Inc. | Method and apparatus for low quantity detection of bioparticles in small sample volumes |
WO2007010367A2 (en) * | 2005-07-19 | 2007-01-25 | Silicon Biosystems S.P.A. | Method and apparatus for the manipulation and/or the detection of particles |
Also Published As
Publication number | Publication date |
---|---|
EP2042239A1 (en) | 2009-04-01 |
WO2009034514A2 (en) | 2009-03-19 |
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