WO2009011942A3 - Methods for rapid multiplexed amplification of target nucleic acids - Google Patents

Methods for rapid multiplexed amplification of target nucleic acids Download PDF

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Publication number
WO2009011942A3
WO2009011942A3 PCT/US2008/059508 US2008059508W WO2009011942A3 WO 2009011942 A3 WO2009011942 A3 WO 2009011942A3 US 2008059508 W US2008059508 W US 2008059508W WO 2009011942 A3 WO2009011942 A3 WO 2009011942A3
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WO
WIPO (PCT)
Prior art keywords
target nucleic
methods
nucleic acids
nucleic acid
peak height
Prior art date
Application number
PCT/US2008/059508
Other languages
French (fr)
Other versions
WO2009011942A2 (en
Inventor
Richard F Selden
Eugene Tan
Heung C Lam
Heidi S Giese
Gregory J Kellogg
John A Wright
Original Assignee
Network Biosystems Inc
Richard F Selden
Eugene Tan
Heung C Lam
Heidi S Giese
Gregory J Kellogg
John A Wright
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Network Biosystems Inc, Richard F Selden, Eugene Tan, Heung C Lam, Heidi S Giese, Gregory J Kellogg, John A Wright filed Critical Network Biosystems Inc
Publication of WO2009011942A2 publication Critical patent/WO2009011942A2/en
Publication of WO2009011942A3 publication Critical patent/WO2009011942A3/en

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    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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Abstract

A fast, multiplexed PCR system is described that can rapidly generate amplified nucleic acid products, for example, a full STR profile, from a target nucleic acid. Such systems include, for example, microfluidic biochips and a custom built thermal cycler, which are also described. The resulting STR profiles can satisfy forensic guidelines for signal strength, inter-loci peak height balance, heterozygous peak height ratio, incomplete non-template nucleotide addition, and stutter.
PCT/US2008/059508 2007-04-04 2008-04-04 Methods for rapid multiplexed amplification of target nucleic acids WO2009011942A2 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US92180207P 2007-04-04 2007-04-04
US60/921,802 2007-04-04
US96450207P 2007-08-13 2007-08-13
US60/964,502 2007-08-13
US2807308P 2008-02-12 2008-02-12
US61/028,073 2008-02-12

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Publication Number Publication Date
WO2009011942A2 WO2009011942A2 (en) 2009-01-22
WO2009011942A3 true WO2009011942A3 (en) 2009-06-11

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PCT/US2008/004405 WO2008124064A1 (en) 2007-04-04 2008-04-04 Plastic microfluidic separation and detection platforms
PCT/US2008/004487 WO2008124116A1 (en) 2007-04-04 2008-04-04 Methods for rapid multiplexed amplification of target nucleic acids
PCT/US2008/059508 WO2009011942A2 (en) 2007-04-04 2008-04-04 Methods for rapid multiplexed amplification of target nucleic acids
PCT/US2008/004462 WO2008124104A1 (en) 2007-04-04 2008-04-04 Integrated nucleic acid analysis

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PCT/US2008/004405 WO2008124064A1 (en) 2007-04-04 2008-04-04 Plastic microfluidic separation and detection platforms
PCT/US2008/004487 WO2008124116A1 (en) 2007-04-04 2008-04-04 Methods for rapid multiplexed amplification of target nucleic acids

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PCT/US2008/004462 WO2008124104A1 (en) 2007-04-04 2008-04-04 Integrated nucleic acid analysis

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US (12) US8018593B2 (en)
EP (7) EP3626345A1 (en)
JP (9) JP2010523115A (en)
KR (4) KR101530943B1 (en)
AU (5) AU2008236691B2 (en)
CA (6) CA3135182C (en)
ES (3) ES2748134T3 (en)
NZ (5) NZ605885A (en)
WO (4) WO2008124064A1 (en)

Families Citing this family (123)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1794581A2 (en) * 2004-09-15 2007-06-13 Microchip Biotechnologies, Inc. Microfluidic devices
GB0421529D0 (en) 2004-09-28 2004-10-27 Landegren Gene Technology Ab Microfluidic structure
JP5063616B2 (en) * 2006-02-03 2012-10-31 インテジェニックス インコーポレイテッド Microfluidic device
ES2688281T3 (en) 2006-07-28 2018-10-31 Diagnostics For The Real World, Ltd Device, system and method to process a sample
CN101715483A (en) 2007-02-05 2010-05-26 微芯片生物工艺学股份有限公司 microfluidic and nanofluidic devices, systems, and applications
KR101530943B1 (en) * 2007-04-04 2015-06-23 네트바이오, 인코포레이티드 Integrated nucleic acid analysis
GB2456079B (en) 2007-08-17 2010-07-14 Diagnostics For The Real World Device, system and method for processing a sample
WO2009108260A2 (en) 2008-01-22 2009-09-03 Microchip Biotechnologies, Inc. Universal sample preparation system and use in an integrated analysis system
CN102056838B (en) 2008-04-11 2013-07-03 弗卢丁公司 Microfluidic device and methods
GB0818609D0 (en) 2008-10-10 2008-11-19 Univ Hull apparatus and method
US8672532B2 (en) 2008-12-31 2014-03-18 Integenx Inc. Microfluidic methods
US8058630B2 (en) * 2009-01-16 2011-11-15 Fluidigm Corporation Microfluidic devices and methods
CA2751455C (en) * 2009-02-03 2019-03-12 Netbio, Inc. Nucleic acid purification
CN102459565A (en) * 2009-06-02 2012-05-16 尹特根埃克斯有限公司 Fluidic devices with diaphragm valves
AU2010257118B2 (en) * 2009-06-04 2014-08-28 Lockheed Martin Corporation Multiple-sample microfluidic chip for DNA analysis
JP2012529268A (en) 2009-06-05 2012-11-22 インテジェンクス，インコーポレイテッド Use of universal sample preparation system and integrated analysis system
WO2010147654A2 (en) 2009-06-15 2010-12-23 Netbio Inc. Improved methods for forensic dna quantitation
US8584703B2 (en) 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
WO2011091473A1 (en) 2010-01-28 2011-08-04 Respirio Pty Ltd Sampling and testing device for the human or animal body
ITMI20100163A1 (en) * 2010-02-03 2011-08-04 Consiglio Nazionale Ricerche PROCEDURE FOR IDENTIFICATION AND TRACEABILITY OF PLANT COMPONENTS
AU2013204367A1 (en) * 2010-03-09 2013-05-16 Netbio, Inc. Unitary biochip providing sample-in to results-out processing and methods of manufacture
NZ721912A (en) 2010-03-09 2018-01-26 Netbio Inc Unitary biochip providing sample-in to results-out processing and methods of manufacture
US8720036B2 (en) 2010-03-09 2014-05-13 Netbio, Inc. Unitary biochip providing sample-in to results-out processing and methods of manufacture
JP5841315B2 (en) 2010-04-28 2016-01-13 ソニー株式会社 Fine particle analyzer
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
US20110312763A1 (en) * 2010-06-17 2011-12-22 Geneasys Pty Ltd Genetic analysis loc with in-loc storage of all required reagents
US9121058B2 (en) 2010-08-20 2015-09-01 Integenx Inc. Linear valve arrays
US8763642B2 (en) 2010-08-20 2014-07-01 Integenx Inc. Microfluidic devices with mechanically-sealed diaphragm valves
US10114020B2 (en) * 2010-10-11 2018-10-30 Mbio Diagnostics, Inc. System and device for analyzing a fluidic sample
GB2497501A (en) 2010-10-15 2013-06-12 Lockheed Corp Micro fluidic optic design
CA2835620C (en) * 2011-05-12 2021-02-23 Netbio, Inc. Methods and compositions for rapid multiplex amplification of str loci
CA2835654A1 (en) 2011-06-01 2012-12-06 Streck, Inc. Rapid thermocycler system for rapid amplification of nucleic acids and related methods
US20130048487A1 (en) * 2011-08-25 2013-02-28 The University Of British Columbia Systems and methods for enrichment and detection of particles
US9207690B2 (en) * 2011-09-23 2015-12-08 Analogic Corporation Thermal cycling
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
CN103160430A (en) 2011-12-13 2013-06-19 三星电子株式会社 Cell capturing filter having high aspect ratio
TWI470224B (en) * 2011-12-22 2015-01-21 Univ Chang Gung Detecting system and detecting method
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
JP6027321B2 (en) * 2012-03-06 2016-11-16 公益財団法人神奈川科学技術アカデミー High-speed gene amplification detector
WO2013169915A1 (en) * 2012-05-08 2013-11-14 Brian Root Self-coating sieving copolymers and plastic devices for nucleic acid separations on such devices
KR102054678B1 (en) * 2012-07-12 2020-01-22 삼성전자주식회사 Fluid analysis cartridge
JP6347780B2 (en) * 2012-07-30 2018-06-27 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Biomolecule detection test strip design
US20140200167A1 (en) 2012-08-01 2014-07-17 Nanomdx, Inc. Functionally integrated device for multiplex genetic identification
CA2879638A1 (en) 2012-08-10 2014-02-13 Streck, Inc. Real-time optical system for polymerase chain reaction
US10890590B2 (en) 2012-09-27 2021-01-12 Ellume Limited Diagnostic devices and methods
WO2014070235A1 (en) 2012-10-29 2014-05-08 Mbio Diagnostics, Inc. Biological particle identification system, cartridge and associated methods
WO2014097287A1 (en) 2012-12-17 2014-06-26 Leukodx, Ltd. Systems and methods for detecting a biological condition
JP6257148B2 (en) * 2013-02-20 2018-01-10 キヤノン株式会社 Image forming apparatus
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
CN111454951A (en) 2013-03-14 2020-07-28 卡里布生物科学公司 Compositions and methods for nucleic acid-targeted nucleic acids
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
WO2014210593A1 (en) 2013-06-28 2014-12-31 Streck, Inc. Devices for real-time polymerase chain reaction
US9259823B2 (en) 2013-08-26 2016-02-16 Lawrence Livermore National Security, Llc Boron nitride composites
JP2015053893A (en) * 2013-09-11 2015-03-23 公益財団法人神奈川科学技術アカデミー High-speed gene amplification detection device
CA2925076C (en) * 2013-09-24 2023-08-29 The Regents Of The University Of California Methods and systems for producing a separation medium using a grayscale mask
WO2015073999A1 (en) 2013-11-18 2015-05-21 Integenx Inc. Cartridges and instruments for sample analysis
US9827566B2 (en) * 2013-11-19 2017-11-28 IDEA machine development design AND production ltd. Multi-well plates and methods of use thereof
US9630182B2 (en) 2013-12-04 2017-04-25 Leidos Innovations Technology, Inc. Non-contact infrared thermocycling
JP2017503175A (en) * 2013-12-31 2017-01-26 キヤノン ユー．エス． ライフ サイエンシズ， インコーポレイテッドＣａｎｏｎ Ｕ．Ｓ． Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ， Ｉｎｃ． Rapid primary results microfluidic system in small format for field deployment
US20150203893A1 (en) * 2014-01-22 2015-07-23 Analogic Corporation Concurrently Evaluating Multiple Disease States Via Processing A Bio-Sample With A Single Multi-Channel Micro-Channel Device
WO2015138343A1 (en) 2014-03-10 2015-09-17 Click Diagnostics, Inc. Cartridge-based thermocycler
GB2591198B (en) * 2014-04-04 2021-10-27 It Is Int Ltd Biochemical reaction system
EP3132073B1 (en) 2014-04-14 2021-06-30 SRI International Portable nucleic acid analysis system and high-performance microfluidic electroactive polymer actuators
GB2544198B (en) 2014-05-21 2021-01-13 Integenx Inc Fluidic cartridge with valve mechanism
CN105277725B (en) * 2014-07-01 2017-03-29 清华大学 A kind of integrated micro-flow control system for foranalysis of nucleic acids detection
CN107106983B (en) 2014-10-22 2021-04-16 尹特根埃克斯有限公司 Systems and methods for sample preparation, processing, and analysis
GB201419879D0 (en) 2014-11-07 2014-12-24 Ibm Self-limited, anisotropic wet etching of transverse vias in microfluidic chips
EP4029606A1 (en) 2014-12-31 2022-07-20 Visby Medical, Inc. Molecular diagnostic testing
US10786229B2 (en) 2015-01-22 2020-09-29 Ellume Limited Diagnostic devices and methods for mitigating hook effect and use thereof
GB2531616B (en) * 2015-02-02 2017-11-22 Atlas Genetics Ltd Instrument for performing a diagnostic test on a fluidic cartridge
GB2553907B (en) * 2015-02-23 2021-06-02 Hitachi High Tech Corp Nucleic acid analyzer
EP4134668A1 (en) * 2015-05-20 2023-02-15 ProteinSimple System and methods for electrophoretic separation and analysis of analytes
US10233491B2 (en) 2015-06-19 2019-03-19 IntegenX, Inc. Valved cartridge and system
JP2017009435A (en) * 2015-06-22 2017-01-12 富士電機株式会社 Heating/cooling mechanism and heating/cooling system
KR101751913B1 (en) * 2015-09-08 2017-07-03 (주) 하임바이오텍 A PCR-based point of care testing device coupled with electrophoretic microfluidic chip
WO2017053531A1 (en) * 2015-09-22 2017-03-30 Bio-Rad Laboratories, Inc. Electrophoresis receptacles and methods
WO2017066485A1 (en) 2015-10-13 2017-04-20 Landers James P Devices and methods for extraction, separation and thermocycling
CN109415722A (en) * 2016-01-29 2019-03-01 普瑞珍生物系统公司 Isotachophoresis for nucleic acid purification
US20170282178A1 (en) * 2016-03-30 2017-10-05 Credo Biomedical Pte Ltd. Portable qpcr and qrt-pcr apparatus
US10987674B2 (en) 2016-04-22 2021-04-27 Visby Medical, Inc. Printed circuit board heater for an amplification module
WO2017197040A1 (en) 2016-05-11 2017-11-16 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
KR101913208B1 (en) * 2016-05-17 2018-10-30 울산대학교 산학협력단 Extracting method of nucleic acid using solid phase object
CN106066303B (en) * 2016-05-24 2019-04-19 中国科学院重庆绿色智能技术研究院 Biomolecule Terahertz characteristic signal receives runner prisms waveguide and preparation method thereof in a kind of enhancing solution
USD800331S1 (en) 2016-06-29 2017-10-17 Click Diagnostics, Inc. Molecular diagnostic device
WO2018005710A1 (en) 2016-06-29 2018-01-04 Click Diagnostics, Inc. Devices and methods for the detection of molecules using a flow cell
USD800913S1 (en) 2016-06-30 2017-10-24 Click Diagnostics, Inc. Detection window for molecular diagnostic device
USD800914S1 (en) 2016-06-30 2017-10-24 Click Diagnostics, Inc. Status indicator for molecular diagnostic device
EP3269444A1 (en) * 2016-07-14 2018-01-17 Base4 Innovation Ltd Method of identifying droplets in a stack and an associated sequencer
DE102016222035A1 (en) * 2016-11-10 2018-05-17 Robert Bosch Gmbh Microfluidic device and method for analyzing samples
DE102017100189A1 (en) * 2017-01-06 2018-07-12 Technische Universität Braunschweig Method for two-dimensional protein separation and protein separation device
KR20200019594A (en) 2017-02-15 2020-02-24 에이엔디이 코포레이션 Systems and Methods for Rapid Nucleic Acid Extraction, Purification and Analysis
US11602750B2 (en) * 2017-05-30 2023-03-14 Roche Molecular Systems, Inc. Customizable sample processing device
WO2019014455A2 (en) 2017-07-12 2019-01-17 Ande Corporation Pattern recognition system
SG11202002931VA (en) 2017-11-09 2020-04-29 Visby Medical Inc Portable molecular diagnostic device and methods for the detection of target viruses
KR102114148B1 (en) * 2018-01-03 2020-05-22 재단법인 구미전자정보기술원 Method for Detecting Foodborne Microorganism
CN108220412B (en) * 2018-01-03 2021-10-01 京东方科技集团股份有限公司 Gene sequencing substrate, preparation method thereof and gene sequencing device
US11150180B1 (en) * 2018-01-16 2021-10-19 Redshift Bioanalytics Inc. Actuated multi-chamber cells for fluid analysis
JP6937851B2 (en) * 2018-01-30 2021-09-22 京セラ株式会社 Measuring device
US10046322B1 (en) * 2018-03-22 2018-08-14 Talis Biomedical Corporation Reaction well for assay device
WO2019182612A1 (en) * 2018-03-23 2019-09-26 Hewlett-Packard Development Company, L.P. Nucleic acid tests with temporal fluorescent signals
WO2019209776A1 (en) 2018-04-27 2019-10-31 Corning Incorporated Microfluidic devices and methods for manufacturing microfluidic devices
CN108645850A (en) * 2018-05-24 2018-10-12 惠州法路源基因科技有限公司 A kind of swab and its preparation method and application
WO2020028273A1 (en) 2018-07-30 2020-02-06 Ande Corporation Multiplexed fuel analysis
CN112566875A (en) * 2018-08-06 2021-03-26 康宁股份有限公司 Microfluidic device and method of manufacturing a microfluidic device
CA3108777C (en) * 2018-08-06 2024-01-16 Siemens Healthcare Diagnostics Inc. Method and device for determining the concentration of analytes in whole blood
CN109813434B (en) * 2018-12-19 2020-07-10 厦门赢科光电有限公司 Human body identification method and device based on temperature detection and terminal equipment
CN111670253A (en) * 2019-01-09 2020-09-15 京东方科技集团股份有限公司 Chip for polymerase chain reaction, operation method thereof and reaction equipment
RU2703776C9 (en) * 2019-01-25 2020-02-18 Российская Федерация в лице Министерства здравоохранения One-time chip for pcr analysis
JP6741100B2 (en) * 2019-02-28 2020-08-19 ソニー株式会社 Microparticle analyzer and microparticle analysis method
EP3941630A4 (en) * 2019-03-18 2023-04-05 Cellular Research, Inc. Precise delivery of components into fluids
US20220112453A1 (en) * 2019-04-30 2022-04-14 Hewlett-Packard Development Company, L.P. Cell lysis with a microbead and thermal resistor
EP4085149A4 (en) 2020-01-03 2024-03-06 Visby Medical Inc Devices and methods for antibiotic susceptibility testing
US20230133186A1 (en) * 2020-03-19 2023-05-04 Mayo Foundation For Medical Education And Research Point-of-care digital microfluidics for sepsis diagnosis
KR102288369B1 (en) * 2020-12-23 2021-08-10 주식회사 미코바이오메드 Integrated chip with lysis and amplifying nucleic acids, lysis module having the same, apparatus for point-of-care-testing having the same, and method for lysis and amplifying nucleic acids using the same
CN112782395B (en) 2020-12-30 2021-11-09 北京中科生仪科技有限公司 PCR detector and method thereof
CN113278509B (en) * 2021-01-15 2022-04-26 北京中科生仪科技有限公司 Chip device for nucleic acid detection
CN112903613B (en) * 2021-02-24 2022-11-08 南昌大学 Labview-based transient absorption spectrum control system design method
WO2023019105A1 (en) * 2021-08-08 2023-02-16 Keydev Inc. Cartridge gel electrophoresis device for separating biomolecules
CN113702317B (en) * 2021-08-30 2023-10-27 中国农业科学院农业信息研究所 River basin non-point source pollution component sensor, monitoring system and method
CN114231401B (en) * 2022-02-21 2022-05-03 北京芯迈微生物技术有限公司 Nucleic acid detection kit and detection method
WO2023168085A1 (en) * 2022-03-04 2023-09-07 Catalog Technologies, Inc. Dna microarrays and component level sequencing for nucleic acid-based data storage and processing
KR20230155183A (en) * 2022-05-03 2023-11-10 경북대학교 산학협력단 A portable device for isolationg nucleic acids in blood

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989009437A1 (en) * 1988-03-26 1989-10-05 Peter Duncan Goodearl Dean Reaction temperature control
US5281516A (en) * 1988-08-02 1994-01-25 Gene Tec Corporation Temperature control apparatus and method
WO1999039005A1 (en) * 1998-01-29 1999-08-05 University Of Pittsburgh Rapid thermocycling for sample analysis
US20030008286A1 (en) * 2001-07-05 2003-01-09 Institute Of Microelectronics Miniaturized multi-chamber thermal cycler for independent thermal multiplexing
US6633785B1 (en) * 1999-08-31 2003-10-14 Kabushiki Kaisha Toshiba Thermal cycler and DNA amplifier method
US20050019902A1 (en) * 1995-09-28 2005-01-27 Mathies Richard A. Miniaturized integrated nucleic acid processing and analysis device and method
US20060068431A1 (en) * 2004-09-27 2006-03-30 Gwo-Bin Lee Micro RT-PCR apparatus and method using the same
EP1710017A1 (en) * 2005-04-04 2006-10-11 Roche Diagnostics GmbH Thermocycling of a block comprising multiple sample

Family Cites Families (284)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2817264A (en) 1953-10-19 1957-12-24 Beckman Instruments Inc Electrophoresis apparatus
US4170616A (en) 1978-05-30 1979-10-09 Rca Corporation Method of fabrication of a Fresnel lens
US5821058A (en) 1984-01-16 1998-10-13 California Institute Of Technology Automated DNA sequencing technique
US5171534A (en) 1984-01-16 1992-12-15 California Institute Of Technology Automated DNA sequencing technique
US5038852A (en) * 1986-02-25 1991-08-13 Cetus Corporation Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
DE3531570A1 (en) * 1985-09-04 1987-03-05 Glotur Trust IGNITION DEVICE FOR INTERNAL COMBUSTION ENGINES
US4855225A (en) * 1986-02-07 1989-08-08 Applied Biosystems, Inc. Method of detecting electrophoretically separated oligonucleotides
US4811218A (en) 1986-06-02 1989-03-07 Applied Biosystems, Inc. Real time scanning electrophoresis apparatus for DNA sequencing
CA1340806C (en) 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US4865707A (en) 1986-10-21 1989-09-12 Northeastern University Capillary gel electrophoresis columns
US5112460A (en) 1986-10-21 1992-05-12 Northeastern University High performance microcapillary gel electrophoresis
JPH0799353B2 (en) 1987-03-31 1995-10-25 株式会社島津製作所 Nucleotide sequencer
JP2550106B2 (en) 1987-10-30 1996-11-06 株式会社日立製作所 Optical dispersion detection type electrophoretic device
US4945135A (en) * 1988-07-25 1990-07-31 California Institute Of Technology Ring opening metathesis polymerization of strained cyclic ethers
CA1339731C (en) 1988-10-12 1998-03-17 Charles T. Caskey Multiplex genomic dna amplification for deletion detection
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
FR2654552A1 (en) 1989-11-14 1991-05-17 Radiotechnique Compelec SEGMENTED PHOTOMULTIPLIER TUBE WITH HIGH COLLECTION EFFICIENCY AND LIMITED DIAPHYT.
US5164055A (en) 1990-01-29 1992-11-17 Applied Biosystems, Inc. High-viscosity polymer matrix and methods
US5858188A (en) 1990-02-28 1999-01-12 Aclara Biosciences, Inc. Acrylic microchannels and their use in electrophoretic applications
US5750015A (en) 1990-02-28 1998-05-12 Soane Biosciences Method and device for moving molecules by the application of a plurality of electrical fields
US5770029A (en) 1996-07-30 1998-06-23 Soane Biosciences Integrated electrophoretic microdevices
US5126022A (en) 1990-02-28 1992-06-30 Soane Tecnologies, Inc. Method and device for moving molecules by the application of a plurality of electrical fields
US5307148A (en) * 1990-04-05 1994-04-26 Hitachi, Ltd. Fluorescence detection type electrophoresis apparatus
JP2826366B2 (en) * 1990-04-12 1998-11-18 株式会社日立製作所 Fluorescence detection type electrophoresis device
US5085756A (en) * 1990-08-21 1992-02-04 Hewlett-Packard Company Column separation system for electrophoresis with sample pretreatment
KR100236506B1 (en) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 Apparatus for polymerase chain reaction
US5332481A (en) 1991-01-29 1994-07-26 Beckman Instruments, Inc. Capillary electrophoresis using replaceable gels
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
DE69213450T2 (en) 1991-06-11 1997-04-17 Nippon Zeon Co HYDROGENATED THERMOPLASTIC NORBORN POLYMER, ITS PRODUCTION, AND BASE BODIES MOLDED OUT THERE FOR OPTICAL ELEMENTS, OPTICAL ELEMENTS AND LENSES
US5334424A (en) 1991-06-17 1994-08-02 Nippon Zeon Co., Ltd. Thermoplastic norbornene resin formed articles and sustrates for liquid crystal display
US5273638A (en) 1991-09-30 1993-12-28 Beckman Instruments, Inc. Nucleotide sequence determination employing matched dideoxynucleotide terminator concentrations
US5274602A (en) * 1991-10-22 1993-12-28 Florida Atlantic University Large capacity solid-state memory
US5198511A (en) 1991-12-20 1993-03-30 Minnesota Mining And Manufacturing Company Polymerizable compositions containing olefin metathesis catalysts and cocatalysts, and methods of use therefor
US5468803A (en) 1992-03-03 1995-11-21 Nippon Zeon Co. Ltd. Medical implement, polymer composition, and optical material
AU3816993A (en) * 1992-03-19 1993-10-21 Regents Of The University Of California, The Multiple tag labeling method for DNA sequencing
US5312940A (en) 1992-04-03 1994-05-17 California Institute Of Technology Ruthenium and osmium metal carbene complexes for olefin metathesis polymerization
US5587128A (en) 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5290418A (en) 1992-09-24 1994-03-01 Applied Biosystems, Inc. Viscous electrophoresis polymer medium and method
CA2110463C (en) 1992-12-17 2005-02-08 Jacob Emert Gel-free ethylene interpolymer dispersant additives useful in oleaginous compositions
US5364790A (en) 1993-02-16 1994-11-15 The Perkin-Elmer Corporation In situ PCR amplification system
EP0620432B1 (en) 1993-04-15 2004-08-25 Zeptosens AG Method for controlling sample introduction in microcolumn separation techniques and sampling device
EP0635994B1 (en) * 1993-07-08 1998-09-23 Canon Kabushiki Kaisha Method and apparatus for separating particles
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US6403367B1 (en) 1994-07-07 2002-06-11 Nanogen, Inc. Integrated portable biological detection system
US5840338A (en) 1994-07-18 1998-11-24 Roos; Eric J. Loading of biologically active solutes into polymer gels
US6001229A (en) 1994-08-01 1999-12-14 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis
US5596226A (en) 1994-09-06 1997-01-21 International Business Machines Corporation Semiconductor chip having a chip metal layer and a transfer metal and corresponding electronic module
US6479235B1 (en) * 1994-09-30 2002-11-12 Promega Corporation Multiplex amplification of short tandem repeat loci
US7008771B1 (en) 1994-09-30 2006-03-07 Promega Corporation Multiplex amplification of short tandem repeat loci
US5843660A (en) 1994-09-30 1998-12-01 Promega Corporation Multiplex amplification of short tandem repeat loci
CA2118048C (en) 1994-09-30 2003-04-08 James W. Schumm Multiplex amplification of short tandem repeat loci
US6485625B1 (en) 1995-05-09 2002-11-26 Curagen Corporation Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
US6017434A (en) 1995-05-09 2000-01-25 Curagen Corporation Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
US5882856A (en) 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
US5856174A (en) * 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5994066A (en) 1995-09-11 1999-11-30 Infectio Diagnostic, Inc. Species-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
US6709869B2 (en) 1995-12-18 2004-03-23 Tecan Trading Ag Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system
US5830657A (en) 1996-05-01 1998-11-03 Visible Genetics Inc. Method for single-tube sequencing of nucleic acid polymers
US5723294A (en) 1996-03-05 1998-03-03 Gull Laboratories Methods for detection and discrimination of multiple analytes using fluorescent technology
CA2252048C (en) 1996-04-12 2008-03-11 The Public Health Research Institute Of The City Of New York, Inc. Detection probes, kits and assays
US5942443A (en) 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
US5885470A (en) 1997-04-14 1999-03-23 Caliper Technologies Corporation Controlled fluid transport in microfabricated polymeric substrates
US6706875B1 (en) 1996-04-17 2004-03-16 Affyemtrix, Inc. Substrate preparation process
US5994064A (en) 1996-04-24 1999-11-30 Identigene, Inc. Simple and complex tandem repeats with DNA typing method
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5847162A (en) * 1996-06-27 1998-12-08 The Perkin Elmer Corporation 4, 7-Dichlororhodamine dyes
JP2000512744A (en) * 1996-05-16 2000-09-26 アフィメトリックス，インコーポレイテッド System and method for detecting label material
US6156512A (en) 1996-05-23 2000-12-05 Promega Corp Allelic ladders for short tandem repeat loci
US6361672B1 (en) 1996-06-10 2002-03-26 Transgenomic, Inc. Multiple laser diode electromagnetic radiation source in multiple electrophoresis channel systems
NZ333346A (en) * 1996-06-28 2000-03-27 Caliper Techn Corp High-throughput screening assay systems in microscale fluidic devices
US5779868A (en) 1996-06-28 1998-07-14 Caliper Technologies Corporation Electropipettor and compensation means for electrophoretic bias
US5858187A (en) 1996-09-26 1999-01-12 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing electrodynamic focusing on a microchip
WO1998017992A2 (en) * 1996-10-25 1998-04-30 Applied Imaging, Inc. Multifluor-fluorescence in situ hybridization (m-fish) imaging techniques using multiple multiband filters with image registration
US6372142B1 (en) * 1996-11-13 2002-04-16 Transgenomic, Inc. Column for DNA separation by matched ion polynucleotide chromatography
US6312886B1 (en) 1996-12-06 2001-11-06 The Secretary Of State For Defence In Her Brittanic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland Reaction vessels
EP0854362A3 (en) 1997-01-16 2000-12-20 Japan Science and Technology Corporation Multi-capillary electrophoresis apparatus
JPH10239278A (en) 1997-02-24 1998-09-11 Hitachi Ltd Cataphoresis device
US6074868A (en) 1997-03-03 2000-06-13 Regents Of The University Of Minnesota Alumina plate method and device for controlling temperature
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
JP2001521394A (en) 1997-04-22 2001-11-06 ババリアン・ノルディック・リサーチ・インスティテュート・アクティーゼルスカブ TaqMan ™ upper TM ™ -PCR for detection of pathogenic E. coli strains
DE19717085C2 (en) 1997-04-23 1999-06-17 Bruker Daltonik Gmbh Processes and devices for extremely fast DNA multiplication using polymerase chain reactions (PCR)
US5976336A (en) 1997-04-25 1999-11-02 Caliper Technologies Corp. Microfluidic devices incorporating improved channel geometries
US7033474B1 (en) * 1997-04-25 2006-04-25 Caliper Life Sciences, Inc. Microfluidic devices incorporating improved channel geometries
WO1998049548A1 (en) 1997-04-25 1998-11-05 Caliper Technologies Corporation Microfluidic devices incorporating improved channel geometries
US5989499A (en) 1997-05-02 1999-11-23 Biomerieux, Inc. Dual chamber disposable reaction vessel for amplification reactions
US6429007B1 (en) 1997-05-02 2002-08-06 BIOMéRIEUX, INC. Nucleic acid amplification reaction station for disposable test devices
US5786182A (en) 1997-05-02 1998-07-28 Biomerieux Vitek, Inc. Dual chamber disposable reaction vessel for amplification reactions, reaction processing station therefor, and methods of use
US6410275B1 (en) 1997-05-02 2002-06-25 Biomerieux, Inc. Disposable test devices for performing nucleic acid amplification reactions
US6632619B1 (en) 1997-05-16 2003-10-14 The Governors Of The University Of Alberta Microfluidic system and methods of use
JP3469585B2 (en) 1997-05-23 2003-11-25 ガメラ バイオサイエンス コーポレイション Apparatus and method for using centripetal acceleration to drive flow motion in microfluidics systems
US5876675A (en) * 1997-08-05 1999-03-02 Caliper Technologies Corp. Microfluidic devices and systems
WO1999014368A2 (en) * 1997-09-15 1999-03-25 Whitehead Institute For Biomedical Research Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device
EP1032824A4 (en) * 1997-10-15 2003-07-23 Aclara Biosciences Inc Laminate microstructure device and method for making same
US6143152A (en) 1997-11-07 2000-11-07 The Regents Of The University Of California Microfabricated capillary array electrophoresis device and method
AU758407B2 (en) 1997-12-24 2003-03-20 Cepheid Integrated fluid manipulation cartridge
US6826524B1 (en) 1998-01-08 2004-11-30 Purdue Research Foundation Sample-adaptive product quantization
US6857449B1 (en) * 1998-01-20 2005-02-22 Caliper Life Sciences, Inc. Multi-layer microfluidic devices
US6167910B1 (en) * 1998-01-20 2001-01-02 Caliper Technologies Corp. Multi-layer microfluidic devices
US6080868A (en) * 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
US6420143B1 (en) 1998-02-13 2002-07-16 Caliper Technologies Corp. Methods and systems for performing superheated reactions in microscale fluidic systems
US6100541A (en) 1998-02-24 2000-08-08 Caliper Technologies Corporation Microfluidic devices and systems incorporating integrated optical elements
CA2230653A1 (en) 1998-02-27 1999-08-27 The Governors Of The University Of Alberta Microchip based enzymatic analysis
US6251247B1 (en) 1998-04-01 2001-06-26 Hitachi Chemical Co., Ltd. Detection of degradation of RNA using microchannel electrophoresis
US6200531B1 (en) 1998-05-11 2001-03-13 Igen International, Inc. Apparatus for carrying out electrochemiluminescence test measurements
US6172997B1 (en) 1998-06-16 2001-01-09 Aculight Corporation Integrated semiconductor diode laser pumped solid state laser
JP2002522097A (en) 1998-08-14 2002-07-23 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Novel amplicons in the 20q13 region of human chromosome 20 and uses thereof
US6605472B1 (en) 1998-10-09 2003-08-12 The Governors Of The University Of Alberta Microfluidic devices connected to glass capillaries with minimal dead volume
JP3613032B2 (en) * 1998-10-26 2005-01-26 株式会社日立製作所 Capillary array electrophoresis device
DE19856064C2 (en) 1998-12-04 2000-11-30 Invitek Gmbh Universal method for the isolation of DNA from any starting material
US6431476B1 (en) * 1999-12-21 2002-08-13 Cepheid Apparatus and method for rapid ultrasonic disruption of cells or viruses
US6887693B2 (en) 1998-12-24 2005-05-03 Cepheid Device and method for lysing cells, spores, or microorganisms
US6261431B1 (en) 1998-12-28 2001-07-17 Affymetrix, Inc. Process for microfabrication of an integrated PCR-CE device and products produced by the same
US7261859B2 (en) * 1998-12-30 2007-08-28 Gyros Ab Microanalysis device
US6249341B1 (en) 1999-01-25 2001-06-19 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
CA2297352A1 (en) 1999-02-05 2000-08-05 Robert J. Lipshutz Multiplex genotyping of populations of individuals
US6558945B1 (en) * 1999-03-08 2003-05-06 Aclara Biosciences, Inc. Method and device for rapid color detection
US6225061B1 (en) 1999-03-10 2001-05-01 Sequenom, Inc. Systems and methods for performing reactions in an unsealed environment
US6303343B1 (en) 1999-04-06 2001-10-16 Caliper Technologies Corp. Inefficient fast PCR
US6235479B1 (en) 1999-04-13 2001-05-22 Bio Merieux, Inc. Methods and devices for performing analysis of a nucleic acid sample
US20050244870A1 (en) 1999-04-20 2005-11-03 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
JP3597729B2 (en) 1999-05-11 2004-12-08 日立ソフトウエアエンジニアリング株式会社 Fluorescence metering method and fluorescence meter
WO2000073413A2 (en) 1999-05-28 2000-12-07 Cepheid Apparatus and method for cell disruption
US6472155B1 (en) 1999-06-08 2002-10-29 The Regents Of The University Of California Species specific identification of spore-producing microbes using the gene sequence of small acid-soluble spore coat proteins for amplification based diagnostics
US6706519B1 (en) * 1999-06-22 2004-03-16 Tecan Trading Ag Devices and methods for the performance of miniaturized in vitro amplification assays
GB9917307D0 (en) 1999-07-23 1999-09-22 Sec Dep Of The Home Department Improvements in and relating to analysis of DNA
US6864050B2 (en) 1999-07-30 2005-03-08 Affymetrix, Inc. Single-phase amplification of nucleic acids
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
GB9922971D0 (en) 1999-09-29 1999-12-01 Secr Defence Reaction system
DE19947495C2 (en) * 1999-10-01 2003-05-28 Agilent Technologies Inc Microfluidic microchip
US6630063B1 (en) * 1999-10-06 2003-10-07 I. Reich Family Limited Partnership Uniform laser excitation and detection in capillary array electrophoresis system and method
EP1146331B1 (en) * 1999-10-20 2008-09-10 Shigeori Takenaka Gene detecting chip, detector, and detecting method
US6471916B1 (en) 1999-11-09 2002-10-29 Packard Instrument Company Apparatus and method for calibration of a microarray scanning system
US6875619B2 (en) 1999-11-12 2005-04-05 Motorola, Inc. Microfluidic devices comprising biochannels
CA2290731A1 (en) 1999-11-26 2001-05-26 D. Jed Harrison Apparatus and method for trapping bead based reagents within microfluidic analysis system
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
US6497977B1 (en) * 1999-11-30 2002-12-24 Ballard Power Systems Inc. Method of making stripped porous polymer films
CA2393690A1 (en) * 1999-12-09 2001-06-14 Huinan Yu Multilayered microfluidic devices for analyte reactions
US6884395B2 (en) * 2000-05-12 2005-04-26 Gyros Ab Integrated microfluidic disc
EP1257668B1 (en) * 2000-02-16 2008-10-29 Illumina, Inc. Parallel genotyping of multiple patient samples
US6329661B1 (en) 2000-02-29 2001-12-11 The University Of Chicago Biochip scanner device
US6407395B1 (en) 2000-02-29 2002-06-18 The University Of Chicago Portable biochip scanner device
ITCA20000004A1 (en) 2000-03-07 2001-09-07 Mario Raimondo Caria METHOD AND SYSTEM FOR SIMULTANEOUS AND MULTIPLE DETECTION WITH QUANTIFICATION OF THE HYBRIDIZATION OF MOLECULAR COMPOUNDS SUCH AS CORE ACIDS
US8288128B2 (en) 2004-11-18 2012-10-16 Eppendorf Array Technologies S.A. Real-time quantification of multiple targets on a micro-array
US6358387B1 (en) 2000-03-27 2002-03-19 Caliper Technologies Corporation Ultra high throughput microfluidic analytical systems and methods
ATE390099T1 (en) * 2000-04-04 2008-04-15 Link Spine Group Inc INTERVERBEL PLASTIC IMPLANT
US6759235B2 (en) 2000-04-06 2004-07-06 Quantum Dot Corporation Two-dimensional spectral imaging system
US7306924B2 (en) 2000-04-17 2007-12-11 Purdue Research Foundation Biosensor and related method
US6733645B1 (en) 2000-04-18 2004-05-11 Caliper Technologies Corp. Total analyte quantitation
US6448014B2 (en) 2000-04-19 2002-09-10 Research Development Foundation PCR-hybridization assays specific for integrated retroviruses
US6787016B2 (en) * 2000-05-01 2004-09-07 Aclara Biosciences, Inc. Dynamic coating with linear polymer mixture for electrophoresis
EP1297179B1 (en) 2000-05-12 2008-09-24 Caliper Life Sciences, Inc. Detection of nucleic acid hybridization by fluorescence polarization
AU2001259770A1 (en) 2000-05-15 2001-11-26 Biomicro Systems, Inc. Air flow regulation in microfluidic circuits for pressure control and gaseous exchange
US6635487B1 (en) * 2000-05-17 2003-10-21 Caliper Technologies Corp. Fluorescence standard for use in microfluidic instruments
US6531282B1 (en) 2000-05-30 2003-03-11 Oligotrail, Llc Multiplex amplification and analysis of selected STR loci
CA2352639A1 (en) 2000-07-14 2002-01-14 John Joseph Cullen A method and apparatus for monitoring a condition in chlorophyll containing matter
EP1305615B1 (en) * 2000-07-21 2004-09-29 Aclara BioSciences, Inc. Method and devices for capillary electrophoresis with a norbornene based surface coating
US7008769B2 (en) 2000-08-15 2006-03-07 Bioforce Nanosciences, Inc. Nanoscale molecular arrayer
US6849407B2 (en) 2000-08-31 2005-02-01 Mayo Foundation For Medical Education And Research Detection of varicella-zoster virus
ATE316581T1 (en) 2000-09-05 2006-02-15 Zeltz Patrick Dr METHOD FOR THE SPECIFIC DETERMINATION OF DNA SEQUENCES USING PARALLEL AMPLIFICATION
US6939451B2 (en) 2000-09-19 2005-09-06 Aclara Biosciences, Inc. Microfluidic chip having integrated electrodes
US6482641B2 (en) 2000-10-02 2002-11-19 Boris Chen DNA-based integrated circuit
AU2001296551A1 (en) * 2000-10-05 2002-04-15 E.I. Du Pont De Nemours And Company Polymeric microfabricated fluidic device suitable for ultraviolet detection
US7294247B1 (en) 2000-10-25 2007-11-13 Xiamen University Electrophoretic separating device and method for using the device
US7033475B2 (en) 2000-10-25 2006-04-25 Shimadzu Corporation Electrophoretic apparatus
AU2002249481A1 (en) 2000-10-25 2002-08-12 Exiqon A/S Open substrate platforms suitable for analysis of biomolecules
US6824024B2 (en) 2000-11-17 2004-11-30 Tecan Trading Ag Device for the take-up and/or release of liquid samples
JP3663125B2 (en) * 2000-11-28 2005-06-22 日立ソフトウエアエンジニアリング株式会社 Fluorescence reading method and fluorescence reading apparatus
US6630680B2 (en) * 2000-12-25 2003-10-07 Fuji Photo Film Co., Ltd. Scanner having confocal optical system, method for producing focus position data of confocal optical system of scanner having confocal optical system and method for producing digital data of scanner having confocal optical system
US6800438B2 (en) * 2000-12-28 2004-10-05 Xerox Corporation Imager for DNA sequencer
AU2002239908A1 (en) 2001-01-12 2002-07-24 Csaba Barta Nanoporous membrane reactor for miniaturized reactions and enhanced reaction kinetics
ATE465272T1 (en) 2001-01-31 2010-05-15 Mayo Foundation DETECTION OF HERPEX SIMPLEX VIRUS
AU2002243764A1 (en) 2001-01-31 2002-08-19 Sau Lan Tang Staats Microfluidic devices
US6790952B2 (en) 2001-02-13 2004-09-14 Cincinnati Children's Hospital Research Foundation Quantitative epstein barr virus PCR rapid assay
CA2445960A1 (en) 2001-02-21 2002-12-19 Amnis Corporation Method and apparatus for labeling and analyzing cellular components
DE10111457B4 (en) 2001-03-09 2006-12-14 Siemens Ag diagnostic device
DE10116674C2 (en) 2001-04-04 2003-08-14 Eppendorf Ag Device for the detection of fluids in a microfluidic component
US6598545B2 (en) 2001-04-10 2003-07-29 Newport Corporation Vibration damper for optical tables and other structures
EP1392814B1 (en) 2001-04-25 2007-06-13 Cornell Research Foundation, Inc. Devices and methods for pharmacokinetic-based cell culture system
FR2823999B1 (en) 2001-04-27 2003-06-27 Commissariat Energie Atomique MINIATURE DEVICE FOR SEPARATING AND ISOLATING BIOLOGICAL OBJECTS AND USES
US6929730B2 (en) 2001-05-01 2005-08-16 Cheng Sheng Lee Two dimensional microfluidic gene scanner
US6596140B2 (en) * 2001-05-01 2003-07-22 Applera Corporation Multi-channel capillary electrophoresis device and method
US20030007895A1 (en) * 2001-06-19 2003-01-09 Fuji Photo Film Co., Ltd. Biochemical analysis unit
US7179423B2 (en) * 2001-06-20 2007-02-20 Cytonome, Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
JPWO2003004982A1 (en) * 2001-07-05 2004-10-28 浜松ホトニクス株式会社 Spectrometer
JP2003028797A (en) * 2001-07-11 2003-01-29 Hitachi Software Eng Co Ltd Fluorescence reader
US20030021016A1 (en) 2001-07-27 2003-01-30 Grier David G. Parallel scanned laser confocal microscope
WO2003012398A1 (en) * 2001-08-03 2003-02-13 Cetek Corporation Microscale affinity purification system
US6919058B2 (en) 2001-08-28 2005-07-19 Gyros Ab Retaining microfluidic microcavity and other microfluidic structures
US6995841B2 (en) 2001-08-28 2006-02-07 Rice University Pulsed-multiline excitation for color-blind fluorescence detection
CA2463689C (en) 2001-10-15 2011-04-26 Nissin Food Products Co., Ltd. Primers and method of detecting bacteria
US6617137B2 (en) 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US20030089605A1 (en) * 2001-10-19 2003-05-15 West Virginia University Research Corporation Microfluidic system for proteome analysis
US7069952B1 (en) 2001-11-14 2006-07-04 Caliper Life Sciences, Inc. Microfluidic devices and methods of their manufacture
US6960298B2 (en) 2001-12-10 2005-11-01 Nanogen, Inc. Mesoporous permeation layers for use on active electronic matrix devices
JP3537802B2 (en) 2001-12-18 2004-06-14 株式会社日立製作所 Electrophoresis chip
JP2003180350A (en) 2001-12-21 2003-07-02 Terumo Corp Temperature control device
JP2003194775A (en) * 2001-12-28 2003-07-09 Japan Science & Technology Corp Electrophoresis method of protein
US7141372B2 (en) 2002-01-18 2006-11-28 Health Research Incorporated Universal RT-coupled PCR method for the specific amplification of mRNA
US7846315B2 (en) 2002-01-28 2010-12-07 Qiagen Sciences, Llc Integrated bio-analysis and sample preparation system
US20040109793A1 (en) 2002-02-07 2004-06-10 Mcneely Michael R Three-dimensional microfluidics incorporating passive fluid control structures
US20030155966A1 (en) 2002-02-20 2003-08-21 Harrison Reid R. Low-power, low-noise CMOS amplifier
DE10208770A1 (en) 2002-02-28 2003-09-04 Febit Ag Increasing the sensitivity and specificity of hybridization experiments with nucleic acid chips
KR100459896B1 (en) 2002-03-06 2004-12-04 삼성전자주식회사 Thermostatic control Method and apparatus for Driving a PCR(polymerize chain reaction) chip
AU2003214155A1 (en) 2002-03-12 2003-09-29 The Regents Of The University Of California Vascularized tissue for transplantation
JP2003271735A (en) 2002-03-12 2003-09-26 Yokogawa Electric Corp Gene diagnosing and analyzing device, and gene diagnosis support system using the same
CA2480200A1 (en) * 2002-04-02 2003-10-16 Caliper Life Sciences, Inc. Methods and apparatus for separation and isolation of components from a biological sample
US6606273B1 (en) 2002-04-11 2003-08-12 Advanced Micro Devices, Inc. Methods and systems for flash memory tunnel oxide reliability testing
US20040009518A1 (en) 2002-05-14 2004-01-15 The Chinese University Of Hong Kong Methods for evaluating a disease condition by nucleic acid detection and fractionation
CA2486812A1 (en) * 2002-05-22 2004-05-21 Platypus Technologies, Llc Substrates, devices, and methods for cellular assays
JP4423189B2 (en) 2002-05-31 2010-03-03 ユィロス・パテント・アクチボラグ Detection device based on surface plasmon resonance
US6806460B2 (en) * 2002-05-31 2004-10-19 Agilent Technologies, Inc. Fluorescence detection with increased dynamic range
US6952651B2 (en) * 2002-06-17 2005-10-04 Intel Corporation Methods and apparatus for nucleic acid sequencing by signal stretching and data integration
US20040018611A1 (en) 2002-07-23 2004-01-29 Ward Michael Dennis Microfluidic devices for high gradient magnetic separation
US7452507B2 (en) 2002-08-02 2008-11-18 Sandia Corporation Portable apparatus for separating sample and detecting target analytes
US20040197845A1 (en) 2002-08-30 2004-10-07 Arjang Hassibi Methods and apparatus for pathogen detection, identification and/or quantification
CA2497288A1 (en) 2002-09-02 2004-03-11 Pamgene B.V. Novel integrated microarray analysis
US6923895B2 (en) * 2002-09-09 2005-08-02 Beckman Coulter, Inc. Coated capillary electrophoresis tubes and system
US6737742B2 (en) 2002-09-11 2004-05-18 International Business Machines Corporation Stacked package for integrated circuits
JPWO2004029241A1 (en) * 2002-09-24 2006-01-26 松下電器産業株式会社 Method for amplifying nucleic acid by electromagnetic induction heating, reaction vessel and reaction apparatus used therefor
US7074598B2 (en) 2002-09-25 2006-07-11 Mayo Foundation For Medical Education And Research Detection of vancomycin-resistant enterococcus spp.
US7074599B2 (en) 2002-09-27 2006-07-11 Mayo Foundation For Medical Education And Research Detection of mecA-containing Staphylococcus spp.
TWI230257B (en) 2002-10-01 2005-04-01 Univ Nat Cheng Kung Integrated analytical biochip and manufacturing method thereof
JP5695287B2 (en) 2002-10-02 2015-04-01 カリフォルニア インスティテュート オブ テクノロジー Nucleic acid analysis of microfluids
JP3813566B2 (en) 2002-10-15 2006-08-23 株式会社エンプラス Resin chip
DE10258398A1 (en) 2002-12-11 2004-08-12 Hans-Knöll-Institut für Naturstoff-Forschung e.V. DNA amplification method
US20050042639A1 (en) 2002-12-20 2005-02-24 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA length
US7445926B2 (en) 2002-12-30 2008-11-04 The Regents Of The University Of California Fluid control structures in microfluidic devices
US20040150217A1 (en) * 2003-01-23 2004-08-05 Heffelfinger David M. Identifying indicia and focusing target
US20040178971A1 (en) 2003-03-10 2004-09-16 Goodart Joseph Edgar Enhanced brightness, color and contrast control for cathode ray tube monitor
DE10315074A1 (en) * 2003-04-02 2004-10-14 Clondiag Chip Technologies Gmbh Device for the duplication and detection of nucleic acids
EP1633481A1 (en) 2003-06-06 2006-03-15 Micronics, Inc. System and method for heating, cooling and heat cycling on microfluidic device
US6864571B2 (en) 2003-07-07 2005-03-08 Gelcore Llc Electronic devices and methods for making same using nanotube regions to assist in thermal heat-sinking
US7205111B2 (en) 2003-07-24 2007-04-17 Marshfield Clinic Rapid identification of bacteria from positive blood cultures
US7150299B2 (en) 2003-09-12 2006-12-19 Air Products And Chemicals, Inc. Assembly and method for containing, receiving and storing fluids and for dispensing gas from a fluid control and gas delivery assembly having an integrated fluid flow restrictor
US7541166B2 (en) 2003-09-19 2009-06-02 Microfluidic Systems, Inc. Sonication to selectively lyse different cell types
JP2007506092A (en) 2003-09-22 2007-03-15 株式会社島津製作所 Electrophoresis apparatus and method, and electrophoresis member and sample dispensing probe
US20050074784A1 (en) 2003-10-07 2005-04-07 Tuan Vo-Dinh Integrated biochip with continuous sampling and processing (CSP) system
JP2005117988A (en) * 2003-10-17 2005-05-12 Thermogen Kk Device for amplifying dna
US20050109621A1 (en) * 2003-11-26 2005-05-26 Peter C. Hauser Method and apparatus for performing high-voltage contactless conductivity (HV-CCD) electrophoresis
GB2416030B (en) 2004-01-28 2008-07-23 Norchip As A diagnostic system for carrying out a nucleic acid sequence amplification and detection process
WO2005080606A1 (en) * 2004-02-18 2005-09-01 Xiaochuan Zhou Fluidic devices and methods for multiplex chemical and biochemical reactions
US7312036B2 (en) 2004-03-22 2007-12-25 Isis Pharmaceuticals, Inc. Compositions for use in identification of viral hemorrhagic fever viruses
DE112004002811T5 (en) 2004-03-30 2008-03-13 Purdue Research Foundation, Lafayette Improved microchannel heat sink
US7280204B2 (en) 2004-04-08 2007-10-09 Purdue Research Foundation Multi-spectral detector and analysis system
WO2005100598A1 (en) 2004-04-19 2005-10-27 Genomictree Inc. Method for preparing a dna chip and use thereof
US20060009106A1 (en) 2004-05-20 2006-01-12 Daiwbo Co., Ltd. Wiping sheet
US7799553B2 (en) 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
JP4911722B2 (en) * 2004-06-07 2012-04-04 フルイディグム コーポレイション Optical lens system and method for microfluidic devices
EP1614466A3 (en) 2004-07-08 2006-05-10 Tecan Trading AG System and method to prevent air bubbles in a hybridization chamber
US7611840B2 (en) 2004-08-03 2009-11-03 Agency For Science, Technology And Research Method and device for the treatment of biological samples
JP4483469B2 (en) * 2004-08-05 2010-06-16 日立電線株式会社 Electrophoresis device
WO2006018044A1 (en) 2004-08-18 2006-02-23 Agilent Technologies, Inc. Microfluidic assembly with coupled microfluidic devices
US8053214B2 (en) 2004-09-09 2011-11-08 Microfluidic Systems, Inc. Apparatus and method of extracting and optically analyzing an analyte from a fluid-based sample
EP1794581A2 (en) 2004-09-15 2007-06-13 Microchip Biotechnologies, Inc. Microfluidic devices
KR100668304B1 (en) 2004-09-16 2007-01-12 삼성전자주식회사 A device for the injection of PCR solution into a PCR channel and a PCR chip unit comprising the device
US20060166223A1 (en) 2005-01-26 2006-07-27 Reed Michael W DNA purification and analysis on nanoengineered surfaces
WO2006099164A2 (en) * 2005-03-10 2006-09-21 Applera Corporation Methods for multiplex amplification
US20060207880A1 (en) * 2005-03-15 2006-09-21 Joyce Timothy H Microfluidic devices and methods of using microfluidic devices
US7275682B2 (en) 2005-03-24 2007-10-02 Varian, Inc. Sample identification utilizing RFID tags
TWI252920B (en) * 2005-03-25 2006-04-11 Ind Tech Res Inst Method of fabricating an integral device of a biochip integrated with micro thermo-electric elements and the apparatus thereof
TW200637919A (en) * 2005-04-21 2006-11-01 Tang Chun Nan With an intelligent controller PCR reactor
WO2006116362A2 (en) 2005-04-25 2006-11-02 The Trustees Of Boston University Structured substrates for optical surface profiling
KR100695148B1 (en) 2005-05-02 2007-03-14 삼성전자주식회사 Module for polymerase chain reaction and multiple polymerase chain reaction system
US20060257958A1 (en) * 2005-05-13 2006-11-16 Pronucleotein Biotechnologies, Llc Magnetically-assisted test strip cartridge and method for using same
US8206974B2 (en) 2005-05-19 2012-06-26 Netbio, Inc. Ruggedized apparatus for analysis of nucleic acid and proteins
WO2006128321A1 (en) 2005-06-02 2006-12-07 Capitalbio Corporation Laser confocal microarray scanner
US7939252B2 (en) * 2005-06-17 2011-05-10 University Of South Carolina Direct quantification of gene expression using capillary electrophoresis with laser-induced fluorescence
US20070014699A1 (en) * 2005-06-23 2007-01-18 Beckman Coulter, Inc, Methods and apparatus for improving the sensitivity of capillary zone electrophoresis
US20070009382A1 (en) * 2005-07-05 2007-01-11 William Bedingham Heating element for a rotating multiplex fluorescence detection device
WO2007021809A2 (en) * 2005-08-11 2007-02-22 Eksigent Technologies, Llc Microfluidic systems, devices and methods for reducing background autofluorescence and the effects thereof
WO2007021814A2 (en) 2005-08-11 2007-02-22 Eksigent Technologies, Llc Plastic surfaces and apparatuses for reduced adsorption of solutes and methods of preparing the same
WO2007044917A2 (en) 2005-10-11 2007-04-19 Handylab, Inc. Polynucleotide sample preparation device
US20070116607A1 (en) 2005-11-23 2007-05-24 Pharmacom Microlelectronics, Inc. Microsystems that integrate three-dimensional microarray and multi-layer microfluidics for combinatorial detection of bioagent at single molecule level
US8097421B2 (en) 2005-12-29 2012-01-17 Intel Corporation Method for performing a multiplex immunoassay using label disassociation and an integrated substrate
US20070154895A1 (en) 2005-12-30 2007-07-05 Caliper Life Sciences, Inc. Multi-assay microfluidic chips
US7715001B2 (en) 2006-02-13 2010-05-11 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
TW200808975A (en) 2006-04-28 2008-02-16 Basf Ag Molecular beacons for DNA-photography
US7629124B2 (en) 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
KR101530943B1 (en) 2007-04-04 2015-06-23 네트바이오, 인코포레이티드 Integrated nucleic acid analysis
EP2265375A1 (en) 2007-10-12 2010-12-29 Rheonix, Inc. Integrated microfluidic device and methods
WO2009108260A2 (en) 2008-01-22 2009-09-03 Microchip Biotechnologies, Inc. Universal sample preparation system and use in an integrated analysis system
CA2751455C (en) 2009-02-03 2019-03-12 Netbio, Inc. Nucleic acid purification
WO2010147654A2 (en) 2009-06-15 2010-12-23 Netbio Inc. Improved methods for forensic dna quantitation
NZ721912A (en) 2010-03-09 2018-01-26 Netbio Inc Unitary biochip providing sample-in to results-out processing and methods of manufacture

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989009437A1 (en) * 1988-03-26 1989-10-05 Peter Duncan Goodearl Dean Reaction temperature control
US5281516A (en) * 1988-08-02 1994-01-25 Gene Tec Corporation Temperature control apparatus and method
US20050019902A1 (en) * 1995-09-28 2005-01-27 Mathies Richard A. Miniaturized integrated nucleic acid processing and analysis device and method
WO1999039005A1 (en) * 1998-01-29 1999-08-05 University Of Pittsburgh Rapid thermocycling for sample analysis
US6633785B1 (en) * 1999-08-31 2003-10-14 Kabushiki Kaisha Toshiba Thermal cycler and DNA amplifier method
US20030008286A1 (en) * 2001-07-05 2003-01-09 Institute Of Microelectronics Miniaturized multi-chamber thermal cycler for independent thermal multiplexing
US20060068431A1 (en) * 2004-09-27 2006-03-30 Gwo-Bin Lee Micro RT-PCR apparatus and method using the same
EP1710017A1 (en) * 2005-04-04 2006-10-11 Roche Diagnostics GmbH Thermocycling of a block comprising multiple sample

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