WO2008122789A3 - Methods for modulating lrrk2 - Google Patents

Methods for modulating lrrk2 Download PDF

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Publication number
WO2008122789A3
WO2008122789A3 PCT/GB2008/001211 GB2008001211W WO2008122789A3 WO 2008122789 A3 WO2008122789 A3 WO 2008122789A3 GB 2008001211 W GB2008001211 W GB 2008001211W WO 2008122789 A3 WO2008122789 A3 WO 2008122789A3
Authority
WO
WIPO (PCT)
Prior art keywords
lrrk2
compound
protein kinase
kinase activity
polypeptide
Prior art date
Application number
PCT/GB2008/001211
Other languages
French (fr)
Other versions
WO2008122789A2 (en
Inventor
Dario Alessi
Original Assignee
Medical Res Council
Dario Alessi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Res Council, Dario Alessi filed Critical Medical Res Council
Priority to EP08718997A priority Critical patent/EP2132326B1/en
Priority to CN200880017563A priority patent/CN101688234A/en
Priority to CA2718580A priority patent/CA2718580A1/en
Priority to DK08718997.3T priority patent/DK2132326T3/en
Priority to JP2010501595A priority patent/JP2010523101A/en
Priority to US12/244,715 priority patent/US7947468B2/en
Publication of WO2008122789A2 publication Critical patent/WO2008122789A2/en
Publication of WO2008122789A3 publication Critical patent/WO2008122789A3/en
Priority to US12/553,932 priority patent/US8206942B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Abstract

A method for identifying a compound expected to be useful in modulating a LRRK2 protein kinase activity, the method comprising the steps of (1) determining whether a test compound modulates the protein kinase activity of a LRRK2 polypeptide on a substrate Ezrin/Radixin/moesin (ERM) family polypeptide and (2) selecting a compound which modulates the LRRK2 polypeptide protein kinase activity. Such a compound may be useful in treating Parkinson's Disease or Parkinsonism. A catalytically active fragment of LRRK2 is identified, requiring the GTPase, COR and kinase domains as well as the WD_40-like motif and C-terminal tail.
PCT/GB2008/001211 2007-04-05 2008-04-07 Methods for modulating lrrk2 WO2008122789A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP08718997A EP2132326B1 (en) 2007-04-05 2008-04-07 Methods
CN200880017563A CN101688234A (en) 2007-04-05 2008-04-07 Methods for modulating lrrk2
CA2718580A CA2718580A1 (en) 2007-04-05 2008-04-07 Methods for identifying kinase modulators
DK08718997.3T DK2132326T3 (en) 2007-04-05 2008-04-07 APPROACHES
JP2010501595A JP2010523101A (en) 2007-04-05 2008-04-07 Adjustment method of LRRK2
US12/244,715 US7947468B2 (en) 2007-04-05 2008-10-02 Methods
US12/553,932 US8206942B2 (en) 2007-04-05 2009-09-03 Methods of identifying LRRK2 inhibitors

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US91024207P 2007-04-05 2007-04-05
GB0706709.3 2007-04-05
US60/910,242 2007-04-05
GBGB0706709.3A GB0706709D0 (en) 2007-04-05 2007-04-05 Methods

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/244,715 Continuation-In-Part US7947468B2 (en) 2007-04-05 2008-10-02 Methods
PCT/GB2009/002047 Continuation-In-Part WO2010031988A2 (en) 2007-04-05 2009-08-24 Methods

Publications (2)

Publication Number Publication Date
WO2008122789A2 WO2008122789A2 (en) 2008-10-16
WO2008122789A3 true WO2008122789A3 (en) 2008-12-04

Family

ID=38090954

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2008/001211 WO2008122789A2 (en) 2007-04-05 2008-04-07 Methods for modulating lrrk2

Country Status (8)

Country Link
US (1) US7947468B2 (en)
EP (1) EP2132326B1 (en)
JP (1) JP2010523101A (en)
CN (1) CN101688234A (en)
CA (1) CA2718580A1 (en)
DK (1) DK2132326T3 (en)
GB (1) GB0706709D0 (en)
WO (1) WO2008122789A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9156845B2 (en) 2012-06-29 2015-10-13 Pfizer Inc. 4-(substituted amino)-7H-pyrrolo[2,3-d] pyrimidines as LRRK2 inhibitors
CN108709997B (en) * 2018-05-28 2020-09-18 中国林业科学研究院林业研究所 Substrate search method for LRR receptor kinase

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0706709D0 (en) 2007-04-05 2007-05-16 Medical Res Council Methods
US8206942B2 (en) 2007-04-05 2012-06-26 Medical Research Council Methods of identifying LRRK2 inhibitors
GB2463656B (en) * 2008-09-18 2010-10-13 Medical Res Council Substrate of LRRK2 and methods of assessing LRRK2 activity
JP2012254937A (en) * 2009-09-11 2012-12-27 Kyushu Univ New matrix peptide of rho-kinase
WO2011036211A1 (en) * 2009-09-23 2011-03-31 Institut National De La Sante Et De La Recherche Medicale (Inserm) Polypeptides and nucleic acids for treating erbb2-dependent cancers
US8367349B2 (en) 2010-04-19 2013-02-05 Medical Research Council Methods for identifying modulators of LRRK2
US9345765B2 (en) 2010-10-08 2016-05-24 Shanghai Kexin Biotech Co., Ltd. Diagnostic and therapeutic uses of moesin fragments
JP6080763B2 (en) * 2010-10-08 2017-02-15 シャンハイ クーシン バイオテック カンパニー，リミテッド Moesin modulator and use thereof
CA2814023C (en) * 2010-10-08 2018-01-16 Shanghai Kexin Biotech Co., Ltd. Moesin fragments and uses thereof
WO2012045279A1 (en) * 2010-10-08 2012-04-12 Shanghai Kexin Biotech Co., Ltd. Moesin fragments associated with immune thrombocytopenia
CA2814026C (en) * 2010-10-08 2017-07-11 Shanghai Kexin Biotech Co., Ltd. Moesin fragments associated with aplastic anemia
BR112013013457A2 (en) * 2010-11-30 2017-07-11 Genentech Inc methods for identifying a patient or subject, for predicting a subject's sensitivity, for identifying subjects who have parkinson's disease, and for determining the subject's response to therapy and assay for selecting compounds and compositions
CA2933767C (en) 2013-12-17 2018-11-06 Pfizer Inc. Novel 3,4-disubstituted-1h-pyrrolo[2,3-b]pyridines and 4,5-disubstituted-7h-pyrrolo[2,3-c]pyridazines as lrrk2 inhibitors
WO2015176010A1 (en) 2014-05-15 2015-11-19 The United States Of America, As Represented By The Secretary, Departmentof Health & Human Services Treatment or prevention of an intestinal disease or disorder
AU2016322813B2 (en) 2015-09-14 2021-04-01 Pfizer Inc. Novel imidazo (4,5-c) quinoline and imidazo (4,5-c)(1,5) naphthyridine derivatives as LRRK2 inhibitors
US20180179594A1 (en) * 2016-10-31 2018-06-28 Parkinson's Institute Multiple system atrophy and the treatment thereof
CN112843067B (en) * 2019-11-26 2023-01-06 兰州大学 Human LRRK2 protein small-molecule inhibitor and application thereof

Citations (3)

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Publication number Priority date Publication date Assignee Title
US20030166222A1 (en) * 2002-01-02 2003-09-04 Millennium Pharmaceuticals, Inc. 39267, human kinase family members and uses therefor
WO2004074485A1 (en) * 2003-02-24 2004-09-02 Research Association For Biotechnology Novel proteins and dnas encoding the same
US20040265849A1 (en) * 2002-11-22 2004-12-30 Applera Corporation Genetic polymorphisms associated with Alzheimer's disease, methods of detection and uses thereof

Family Cites Families (3)

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Publication number Priority date Publication date Assignee Title
AU2007224411B2 (en) 2006-03-14 2012-11-01 Cellzome Gmbh Methods for the identification of LRRK2 interacting molecules and for the purification of LRRK2
WO2008091799A2 (en) 2007-01-22 2008-07-31 The Trustees Of Columbia University In The City Of New York Cell-based methods for identifying inhibitors of parkinson's disease-associated lrrk2 mutants
GB0706709D0 (en) 2007-04-05 2007-05-16 Medical Res Council Methods

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030166222A1 (en) * 2002-01-02 2003-09-04 Millennium Pharmaceuticals, Inc. 39267, human kinase family members and uses therefor
US20040265849A1 (en) * 2002-11-22 2004-12-30 Applera Corporation Genetic polymorphisms associated with Alzheimer's disease, methods of detection and uses thereof
WO2004074485A1 (en) * 2003-02-24 2004-09-02 Research Association For Biotechnology Novel proteins and dnas encoding the same

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE UniProt [online] 11 October 2005 (2005-10-11), "SubName: Full=Putative uncharacterized protein; Flags: Fragment;", XP002494863, retrieved from EBI accession no. UNIPROT:Q3UF33 Database accession no. Q3UF33 *
JALEEL MAHABOOBI ET AL: "LRRK2 phosphorylates moesin at threonine-558: characterization of how Parkinson's disease mutants affect kinase activity", BIOCHEMICAL JOURNAL, vol. 405, no. Part 2, July 2007 (2007-07-01), pages 307 - 317, XP002494862, ISSN: 0264-6021(print) 1470-8728(ele *
KATAYAMA S ET AL: "Antisense transcription in the mammalian transcriptome", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, WASHINGTON, DC, vol. 309, no. 5740, 2 September 2005 (2005-09-02), pages 1564 - 1566, XP003020135, ISSN: 0036-8075 *
MATA ET AL: "LRRK2 in Parkinson's disease: protein domains and functional insights", TRENDS IN NEUROSCIENCE, ELSEVIER, AMSTERDAM, NL, vol. 29, no. 5, 1 May 2006 (2006-05-01), pages 286 - 293, XP005415125, ISSN: 0166-2236 *
MATSUI T ET AL: "RHO-KINASE PHOSPHORYLATES COOH-TERMINAL THREONINES OF EZRIN/RADIXIN/MOESIN (ERM) PROTEINS AND REGULATES THEIR HEAD-TO-TAIL ASSOCIATION", THE JOURNAL OF CELL BIOLOGY, ROCKEFELLER UNIVERSITY PRESS, US, vol. 140, no. 3, 9 February 1998 (1998-02-09), pages 647 - 657, XP001083757, ISSN: 0021-9525 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9156845B2 (en) 2012-06-29 2015-10-13 Pfizer Inc. 4-(substituted amino)-7H-pyrrolo[2,3-d] pyrimidines as LRRK2 inhibitors
CN108709997B (en) * 2018-05-28 2020-09-18 中国林业科学研究院林业研究所 Substrate search method for LRR receptor kinase

Also Published As

Publication number Publication date
JP2010523101A (en) 2010-07-15
EP2132326A2 (en) 2009-12-16
GB0706709D0 (en) 2007-05-16
US20090142784A1 (en) 2009-06-04
US7947468B2 (en) 2011-05-24
CA2718580A1 (en) 2008-10-16
WO2008122789A2 (en) 2008-10-16
DK2132326T3 (en) 2012-12-17
EP2132326B1 (en) 2012-10-31
CN101688234A (en) 2010-03-31

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