WO2008070276A3 - Isolation op osteoinductive proteins - Google Patents

Isolation op osteoinductive proteins Download PDF

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Publication number
WO2008070276A3
WO2008070276A3 PCT/US2007/081034 US2007081034W WO2008070276A3 WO 2008070276 A3 WO2008070276 A3 WO 2008070276A3 US 2007081034 W US2007081034 W US 2007081034W WO 2008070276 A3 WO2008070276 A3 WO 2008070276A3
Authority
WO
WIPO (PCT)
Prior art keywords
molecular weight
osteoinductive proteins
ultrafiltration
proteins
kilodaltons
Prior art date
Application number
PCT/US2007/081034
Other languages
French (fr)
Other versions
WO2008070276A2 (en
Inventor
Kevin Thorne
Rama Akella
Original Assignee
Zimmer Inc
Kevin Thorne
Rama Akella
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zimmer Inc, Kevin Thorne, Rama Akella filed Critical Zimmer Inc
Priority to EP07871149A priority Critical patent/EP2084177A2/en
Publication of WO2008070276A2 publication Critical patent/WO2008070276A2/en
Publication of WO2008070276A3 publication Critical patent/WO2008070276A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18—Growth factors; Growth regulators
    • A61K38/1875—Bone morphogenic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/51—Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827—Proteins from mammals or birds
    • Y10S530/84—Bones; tendons; teeth; cartilage

Abstract

A method for purifying bone-derived osteoinductive proteins including a demineralization process, a protein extraction process, a high molecular weight ultrafiltration process, a low molecular weight ultrafiltration process, and a recovery process. The high and low ultrafiltration processes preferably select proteins having a nominal molecular weight between approximately 8 kilodaltons and approximately 100 kilodaltons. Processes of the present invention may be used to recover osteoinductive proteins from bone demineralization waste streams.
PCT/US2007/081034 2006-10-27 2007-10-11 Isolation op osteoinductive proteins WO2008070276A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP07871149A EP2084177A2 (en) 2006-10-27 2007-10-11 Isolation of osteoinductive proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/553,640 2006-10-27
US11/553,640 US7622562B2 (en) 2002-06-26 2006-10-27 Rapid isolation of osteoinductive protein mixtures from mammalian bone tissue

Publications (2)

Publication Number Publication Date
WO2008070276A2 WO2008070276A2 (en) 2008-06-12
WO2008070276A3 true WO2008070276A3 (en) 2008-10-23

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/081034 WO2008070276A2 (en) 2006-10-27 2007-10-11 Isolation op osteoinductive proteins

Country Status (3)

Country Link
US (2) US7622562B2 (en)
EP (1) EP2084177A2 (en)
WO (1) WO2008070276A2 (en)

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US7901457B2 (en) 2003-05-16 2011-03-08 Musculoskeletal Transplant Foundation Cartilage allograft plug
US7837740B2 (en) 2007-01-24 2010-11-23 Musculoskeletal Transplant Foundation Two piece cancellous construct for cartilage repair
US7815926B2 (en) 2005-07-11 2010-10-19 Musculoskeletal Transplant Foundation Implant for articular cartilage repair
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US8435551B2 (en) 2007-03-06 2013-05-07 Musculoskeletal Transplant Foundation Cancellous construct with support ring for repair of osteochondral defects
US20110081682A1 (en) * 2007-11-14 2011-04-07 Osteosphere, Llc Production of bone morphogenic proteins (bmps) using a novel tissue culture platform
US20090136929A1 (en) * 2007-11-28 2009-05-28 Zimmer Orthobiologics, Inc. Novel in vitro method of quantifying demineralized bone osteoinductivity
CA2717725A1 (en) 2008-03-05 2009-09-11 Musculoskeletal Transplant Foundation Cancellous constructs, cartilage particles and combinations of cancellous constructs and cartilage particles
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US9192695B2 (en) 2008-11-20 2015-11-24 Allosource Allografts combined with tissue derived stem cells for bone healing
WO2012000930A1 (en) 2010-06-28 2012-01-05 Bbs-Bioactive Bone Substitutes Oy A method for preparing a bone protein preparation and a bone protein preparation
US8920511B2 (en) 2011-11-17 2014-12-30 Allosource Multi-piece machine graft systems and methods
AU2014225458A1 (en) 2013-03-07 2015-07-09 Allosource Consistent calcium content bone allograft systems and methods
EP2970882B1 (en) 2013-03-15 2018-11-28 AlloSource Cell repopulated collagen matrix for soft tissue repair and regeneration
US9730796B2 (en) 2014-05-16 2017-08-15 Allosource Composite bone constructs and methods
US10077420B2 (en) 2014-12-02 2018-09-18 Histogenics Corporation Cell and tissue culture container
KR20240018687A (en) 2017-06-30 2024-02-13 알로소스 Cellular bone grafts, and methods of manufacture and use
CN112089891A (en) * 2020-09-22 2020-12-18 天津中津生物发展有限公司 Bone induction active extract and preparation method thereof
US20230040249A1 (en) * 2021-08-02 2023-02-09 Seaspine, Inc. Method of Demineralizing Bone with an Ionic Solution
WO2023092236A1 (en) * 2021-11-26 2023-06-01 Red Rock Regeneration Inc. Treatment of bone tissue to increase the bioavailability of growth factors
CN114276407B (en) * 2021-12-29 2023-08-15 浙江旅游职业学院 Device and method for extracting protein from bone or shell by pH shift technology

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