WO2008048663A3 - Novel process for devitalized/acellular tissue for transplantation - Google Patents

Novel process for devitalized/acellular tissue for transplantation Download PDF

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Publication number
WO2008048663A3
WO2008048663A3 PCT/US2007/022225 US2007022225W WO2008048663A3 WO 2008048663 A3 WO2008048663 A3 WO 2008048663A3 US 2007022225 W US2007022225 W US 2007022225W WO 2008048663 A3 WO2008048663 A3 WO 2008048663A3
Authority
WO
WIPO (PCT)
Prior art keywords
tissue
devitalized
transplantation
processing
novel process
Prior art date
Application number
PCT/US2007/022225
Other languages
French (fr)
Other versions
WO2008048663A2 (en
Inventor
Nancy Jane Shelby
Kristin Leigh Fitzpatrick
Darrel Lee Holmes
Original Assignee
Bacterin Int Inc
Nancy Jane Shelby
Kristin Leigh Fitzpatrick
Darrel Lee Holmes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bacterin Int Inc, Nancy Jane Shelby, Kristin Leigh Fitzpatrick, Darrel Lee Holmes filed Critical Bacterin Int Inc
Priority to US12/311,964 priority Critical patent/US20100028849A1/en
Publication of WO2008048663A2 publication Critical patent/WO2008048663A2/en
Publication of WO2008048663A3 publication Critical patent/WO2008048663A3/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/0231—Chemically defined matrices, e.g. alginate gels, for immobilising, holding or storing cells, tissue or organs for preservation purposes; Chemically altering or fixing cells, tissue or organs, e.g. by cross-linking, for preservation purposes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds

Abstract

A method for processing tissue to produce a devitalized acellular matrix for transplantation comprises soaking the tissue in a first processing solution having a pH below 7 to reduce protease activity, periodically infusing ozone into the first processing solution to devitalize the tissue and reduce bioburden, and soaking the tissue in a second processing solution to remove cellular debris. The present invention also includes a system for processing the tissue. The devitalized acellular matrix acts as a scaffold for cellular ingrowth when transplanted into a recipient to form new tissue.
PCT/US2007/022225 2006-10-18 2007-10-18 Novel process for devitalized/acellular tissue for transplantation WO2008048663A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12/311,964 US20100028849A1 (en) 2006-10-18 2007-10-18 Novel process for devitalized/acellular tissue for transplantation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US85291506P 2006-10-18 2006-10-18
US60/852,915 2006-10-18

Publications (2)

Publication Number Publication Date
WO2008048663A2 WO2008048663A2 (en) 2008-04-24
WO2008048663A3 true WO2008048663A3 (en) 2008-06-05

Family

ID=39314675

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/022225 WO2008048663A2 (en) 2006-10-18 2007-10-18 Novel process for devitalized/acellular tissue for transplantation

Country Status (2)

Country Link
US (1) US20100028849A1 (en)
WO (1) WO2008048663A2 (en)

Families Citing this family (22)

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EP3311826B1 (en) 2011-02-14 2020-04-29 MiMedx Group, Inc. Micronized placental tissue compositions and methods for making and using the same
US20140205646A1 (en) 2011-02-14 2014-07-24 Mimedx Group, Inc. Tissue grafts modified with a cross-linking agent and method of making and using the same
EP2793745B1 (en) 2011-12-22 2019-07-31 MIMEDX Group Inc. Cross-linked dehydrated placental tissue grafts and methods for making and using the same
US11338063B2 (en) 2012-08-15 2022-05-24 Mimedx Group, Inc. Placental tissue grafts modified with a cross-linking agent and methods of making and using the same
US8904664B2 (en) 2012-08-15 2014-12-09 Mimedx Group, Inc. Dehydration device and methods for drying biological materials
CA2880157C (en) 2012-08-15 2020-07-21 Mimedx Group, Inc. Reinforced placental tissue grafts and methods of making and using the same
US9943551B2 (en) 2012-08-15 2018-04-17 Mimedx Group, Inc. Tissue grafts composed of micronized placental tissue and methods of making and using the same
US9180145B2 (en) 2012-10-12 2015-11-10 Mimedx Group, Inc. Compositions and methods for recruiting and localizing stem cells
US9155799B2 (en) 2012-11-19 2015-10-13 Mimedx Group, Inc. Cross-linked collagen with at least one bound antimicrobial agent for in vivo release of the agent
US8946163B2 (en) 2012-11-19 2015-02-03 Mimedx Group, Inc. Cross-linked collagen comprising metallic anticancer agents
US9827293B2 (en) 2013-01-17 2017-11-28 Mimedx Group, Inc. Non-surgical, localized delivery of compositions for placental growth factors
US10517931B2 (en) 2013-01-17 2019-12-31 Mimedx Group, Inc. Non-surgical, localized delivery of compositions for placental growth factors
US9655948B1 (en) 2013-01-17 2017-05-23 Mimedx Group, Inc. Non-surgical, localized delivery of compositions for placental growth factors
US10206977B1 (en) 2013-01-18 2019-02-19 Mimedx Group, Inc. Isolated placental stem cell recruiting factors
JP6545102B2 (en) 2013-01-18 2019-07-17 ミメディクス グループ インコーポレイテッド How to treat a cardiac condition
US10029030B2 (en) 2013-03-15 2018-07-24 Mimedx Group, Inc. Molded placental tissue compositions and methods of making and using the same
US9446142B2 (en) 2013-05-28 2016-09-20 Mimedx Group, Inc. Polymer chelator conjugates
WO2015031681A1 (en) 2013-08-30 2015-03-05 Mimedx Group, Inc. Micronized placental compositions comprising a chelator
EP3094336A4 (en) 2014-01-17 2018-02-14 MIMEDX Group Inc. Method for inducing angiogenesis
EP3185918B1 (en) 2014-08-28 2021-08-18 MiMedx Group, Inc. Collagen reinforced tissue grafts
CN104307044B (en) * 2014-10-13 2016-06-08 浙江大学医学院附属邵逸夫医院 A kind of preparation method of the de-cell material of total spinal disc in natural tissues source
EP4272830A3 (en) * 2016-12-09 2024-02-14 The University of Chicago Tissue organoids

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5328821A (en) * 1991-12-12 1994-07-12 Robyn Fisher Cold and cryo-preservation methods for human tissue slices
US5403742A (en) * 1993-09-01 1995-04-04 Ramot University Authority Ltd. Bioreactor for production of products with immobilized biofilm
US5613982A (en) * 1994-03-14 1997-03-25 Cryolife, Inc. Method of preparing transplant tissue to reduce immunogenicity upon implantation
US20030156978A1 (en) * 2001-11-26 2003-08-21 Gillette Thomas D. Method and device for providing ozone sanitation of various objects
US6736833B2 (en) * 2001-06-07 2004-05-18 Hospital For Special Surgery Application of UV to collagen for altering length and properties of tissue
US20040176855A1 (en) * 2003-03-07 2004-09-09 Acell, Inc. Decellularized liver for repair of tissue and treatment of organ deficiency
US20060210960A1 (en) * 1990-09-12 2006-09-21 Lifecell Corporation, A Texas Corporation Method for processing and preserving collagen-based tissues for transplantation
US20060228252A1 (en) * 2004-04-20 2006-10-12 Mills C R Process and apparatus for treating implants comprising soft tissue

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060210960A1 (en) * 1990-09-12 2006-09-21 Lifecell Corporation, A Texas Corporation Method for processing and preserving collagen-based tissues for transplantation
US5328821A (en) * 1991-12-12 1994-07-12 Robyn Fisher Cold and cryo-preservation methods for human tissue slices
US5403742A (en) * 1993-09-01 1995-04-04 Ramot University Authority Ltd. Bioreactor for production of products with immobilized biofilm
US5613982A (en) * 1994-03-14 1997-03-25 Cryolife, Inc. Method of preparing transplant tissue to reduce immunogenicity upon implantation
US6736833B2 (en) * 2001-06-07 2004-05-18 Hospital For Special Surgery Application of UV to collagen for altering length and properties of tissue
US20030156978A1 (en) * 2001-11-26 2003-08-21 Gillette Thomas D. Method and device for providing ozone sanitation of various objects
US20040176855A1 (en) * 2003-03-07 2004-09-09 Acell, Inc. Decellularized liver for repair of tissue and treatment of organ deficiency
US20060228252A1 (en) * 2004-04-20 2006-10-12 Mills C R Process and apparatus for treating implants comprising soft tissue

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GREENER ET AL.: "Proteases and pH in chronic wounds", JOURNAL OF WOUND CARE, vol. 14, no. 2, 1 February 2005 (2005-02-01), pages 59 - 61, XP009082757 *

Also Published As

Publication number Publication date
US20100028849A1 (en) 2010-02-04
WO2008048663A2 (en) 2008-04-24

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