WO2007149724A2 - Substrates for performing analytical reactions - Google Patents
Substrates for performing analytical reactions Download PDFInfo
- Publication number
- WO2007149724A2 WO2007149724A2 PCT/US2007/070901 US2007070901W WO2007149724A2 WO 2007149724 A2 WO2007149724 A2 WO 2007149724A2 US 2007070901 W US2007070901 W US 2007070901W WO 2007149724 A2 WO2007149724 A2 WO 2007149724A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- layer
- zero mode
- mode waveguide
- core
- cladding layer
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
- G02B6/10—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/7703—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator using reagent-clad optical fibres or optical waveguides
- G01N2021/7706—Reagent provision
- G01N2021/7709—Distributed reagent, e.g. over length of guide
- G01N2021/7713—Distributed reagent, e.g. over length of guide in core
Definitions
- ZM VV arrays have been applied in a range of biochemical analyses and particularly in the field of genetic analysis.
- ZMWs typically comprise a nanoscale core, well or opening disposed in an opaque cladding layer that is disposed upon a transparent substrate. Due to the narrow dimensions of the core electromagnetic radiation that is of a frequenc ⁇ above a particular cut-off frequency w ilt be prevented from propagating all the way through the core. Notwithstanding the foregoing, the radiation w ill penetrate a limited distance into the core, providing a very small illuminated volume w ithin the core. By illuminating a v ery small volume, one can potentially interrogate verv small quantities of reagents, including, e.g.. single molecule reactions.
- the invention provides a zero mode waveguide substrate that comprises a transparent substrate layer having at least a first surface, a metal cladding layer disposed upon the first surface of the transparent substrate layer and an aperture disposed through the cladding layer to the transparent substrate layer, and forming a core region surrounded by the metal cladding layer, wherein the core is dimensioned to prevent electromagnetic radiation of a frequency greater than a cut-off frequency from propagating entirely through the core.
- the substrate also includes a sacrificial anode disposed upon the cladding layer.
- Figure 2 provides a schematic illustration of a ZMW including a sacrificial anode layer.
- analytical substrates generally comprise a rigid or semi-rigid solid substrate, typically substantially planar in overall configuration, upon which reactions of interest may be carried out and monitored, using an associated detection system, such as a fluorescence microscope, optical imaging system, or electrical signal detection system.
- an associated detection system such as a fluorescence microscope, optical imaging system, or electrical signal detection system.
- substrates may have additional functional components manufactured into the substrate or disposed upon a surface of the substrate.
- Such components include, e.g., molecular components, such as molecular binding moieties, e.g., antibodies, nucleic acids non-specific chemical coupling groups, binding peptides, or the like, or property altering groups such as hydrophobic or hydrophilic groups, to provide areas of relative hydrophobicity or h ⁇ drophilicity on the substrates.
- the substrates may have structural components fabricated onto or into the substrates, including, e.g., barriers, wells, posts, pillars, channels, troughs, electrical contacts, mask lavers, cladding layers or the like.
- the zero mode waveguides of the invention are typically fabricated into a substrate that includes a transparent substrate layer and an opaque cladding layer deposited upon the transparent substrate.
- the core portion of the waveguides comprises an aperture disposed through the cladding layer to the transparent layer to define a well having an open upper end and a lower end that is capped by the transparent substrate.
- a schematic illustration of such waveguides is shown in Figure 1.
- the overall substrate 100 includes a waveguide core 102 disposed within opaque cladding layer 104 and continuing through to the transparent substrate layer 106.
- ZMW fabrication can be carried out by a number of methods.
- the zinc layer may generally be co-deposited with the metal cladding layer or deposited as a subsequent layer on top of the metai cladding layer.
- the waveguide core would be disposed through both the galvanic anode and metal cladding layers.
- the anode layer will be from about 0.1 nm to about 100 nm thick, preferably from about 1 to about 100 nm thick, from about 1 to about 50 nm thick and in some cases from about 10 to about 50 nm thick.
- An example of a waveguide substrate in accordance with this aspect of the invention is shown in Figure 2.
- the overall ZMW device 200 again includes a core 102 disposed in cladding layer 104 that is. in turn, disposed on the surface of transparent substrate 106.
- a sacrificial anode layer 208 is disposed upon the upper surface of the cladding layer 104, and serves to prevent excessive galvanic corrosion of the metal cladding layer during application.
- a ZMW substrate 600 includes a core 602 disposed within a cladding layer 604 through to the transparent substrate layer 606.
- a transparent substrate 400 is provided having an upper surface 402.
- a resist layer 404 is then deposited over the surface 402.
- the resist is exposed and developed to yield a negative image of the desired waveguide cores, shown as pillars 406.
- a lajer of additional transparent material 408 is then deposited upon the exposed surface 402 of the substrate to build up the transparent substrate la>er around the pillar 406.
- the transparent material is preferabiy the same or similar in composition to the underlying transparent substrate 400.
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2007800218264A CN101467082B (en) | 2006-06-12 | 2007-06-11 | Substrates for performing analytical reactions |
JP2009515592A JP2009539411A (en) | 2006-06-12 | 2007-06-11 | Substrates for performing analytical reactions |
EP07845184.6A EP2027253A4 (en) | 2006-06-12 | 2007-06-11 | Substrates for performing analytical reactions |
CA2654470A CA2654470C (en) | 2006-06-12 | 2007-06-11 | Substrates for performing analytical reactions |
AU2007261114A AU2007261114B2 (en) | 2006-06-12 | 2007-06-11 | Substrates for performing analytical reactions |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US81286306P | 2006-06-12 | 2006-06-12 | |
US60/812,863 | 2006-06-12 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2007149724A2 true WO2007149724A2 (en) | 2007-12-27 |
WO2007149724A3 WO2007149724A3 (en) | 2008-07-31 |
Family
ID=38834243
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2007/070901 WO2007149724A2 (en) | 2006-06-12 | 2007-06-11 | Substrates for performing analytical reactions |
Country Status (7)
Country | Link |
---|---|
US (2) | US7486865B2 (en) |
EP (2) | EP2027253A4 (en) |
JP (1) | JP2009539411A (en) |
CN (2) | CN101467082B (en) |
AU (1) | AU2007261114B2 (en) |
CA (1) | CA2654470C (en) |
WO (1) | WO2007149724A2 (en) |
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CN106148969A (en) * | 2014-12-29 | 2016-11-23 | 厦门火炬特种金属材料有限公司 | A kind of sacrificial magnesium alloy anode current efficiency device for fast detecting and method |
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- 2007-06-11 EP EP07845184.6A patent/EP2027253A4/en not_active Withdrawn
- 2007-06-11 WO PCT/US2007/070901 patent/WO2007149724A2/en active Application Filing
- 2007-06-11 US US11/761,251 patent/US7486865B2/en active Active
- 2007-06-11 CN CN2007800218264A patent/CN101467082B/en active Active
- 2007-06-11 CN CN2010102565357A patent/CN101915957B/en active Active
- 2007-06-11 AU AU2007261114A patent/AU2007261114B2/en active Active
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008078263A1 (en) | 2006-12-21 | 2008-07-03 | Koninklijke Philips Electronics N.V. | Aperture biosensor with trenches |
JP2010513909A (en) * | 2006-12-21 | 2010-04-30 | コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ | Opening biosensor with grooves |
US9176062B2 (en) | 2006-12-21 | 2015-11-03 | Koninklijke Philips N.V. | Aperture biosensor with trenches |
WO2008122267A2 (en) * | 2007-04-04 | 2008-10-16 | Synentec Gmbh | Biochip for carrying out a fluorescence analysis of individual transporters |
WO2008122267A3 (en) * | 2007-04-04 | 2008-12-11 | Synentec Gmbh | Biochip for carrying out a fluorescence analysis of individual transporters |
CN106148969A (en) * | 2014-12-29 | 2016-11-23 | 厦门火炬特种金属材料有限公司 | A kind of sacrificial magnesium alloy anode current efficiency device for fast detecting and method |
Also Published As
Publication number | Publication date |
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AU2007261114B2 (en) | 2012-07-12 |
EP2027253A4 (en) | 2014-04-30 |
EP2027253A2 (en) | 2009-02-25 |
CN101915957B (en) | 2012-12-12 |
WO2007149724A3 (en) | 2008-07-31 |
AU2007261114A1 (en) | 2007-12-27 |
EP2857829B1 (en) | 2017-08-09 |
CN101915957A (en) | 2010-12-15 |
CN101467082B (en) | 2011-12-14 |
US20080095488A1 (en) | 2008-04-24 |
JP2009539411A (en) | 2009-11-19 |
US7907800B2 (en) | 2011-03-15 |
CA2654470A1 (en) | 2007-12-27 |
CA2654470C (en) | 2015-02-03 |
EP2857829A1 (en) | 2015-04-08 |
US7486865B2 (en) | 2009-02-03 |
US20090325166A1 (en) | 2009-12-31 |
CN101467082A (en) | 2009-06-24 |
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