WO2007141811A3 - Process for preparing a semiconductor substrate for biological analysis - Google Patents

Process for preparing a semiconductor substrate for biological analysis Download PDF

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Publication number
WO2007141811A3
WO2007141811A3 PCT/IT2006/000420 IT2006000420W WO2007141811A3 WO 2007141811 A3 WO2007141811 A3 WO 2007141811A3 IT 2006000420 W IT2006000420 W IT 2006000420W WO 2007141811 A3 WO2007141811 A3 WO 2007141811A3
Authority
WO
WIPO (PCT)
Prior art keywords
silicon dioxide
semiconductor substrate
biological analysis
silane
treating
Prior art date
Application number
PCT/IT2006/000420
Other languages
French (fr)
Other versions
WO2007141811A2 (en
Inventor
Andrea Frosini
Alessandra Fischetti
Original Assignee
St Microelectronics Srl
Andrea Frosini
Alessandra Fischetti
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by St Microelectronics Srl, Andrea Frosini, Alessandra Fischetti filed Critical St Microelectronics Srl
Priority to PCT/IT2006/000420 priority Critical patent/WO2007141811A2/en
Publication of WO2007141811A2 publication Critical patent/WO2007141811A2/en
Publication of WO2007141811A3 publication Critical patent/WO2007141811A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Abstract

A process for preparing a semiconductor substrate for biological analysis in an integrated device, the biological analysis comprising the steps of amplifying DNA and detecting amplified DNA in the same chamber, comprises the steps of a) forming a silicon dioxide surface on said semiconductor substrate b) treating said silicon dioxide surface with a silane; c) forming a silanized surface; d) grafting nucleic acid probes; e) treating said silanized surface with a deactivating agent and f) forming a deactivated substrate sequentially. Further the process can include the step of cleaning the silicon dioxide substrate before the step of treating said silicon dioxide surface with a silane and the step of reacting the terminal group of the silane with a cross-linker or alternatively the step of reacting the derivatized nucleic acid probes with a cross-linker, before the grafting step.
PCT/IT2006/000420 2006-06-06 2006-06-06 Process for preparing a semiconductor substrate for biological analysis WO2007141811A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/IT2006/000420 WO2007141811A2 (en) 2006-06-06 2006-06-06 Process for preparing a semiconductor substrate for biological analysis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IT2006/000420 WO2007141811A2 (en) 2006-06-06 2006-06-06 Process for preparing a semiconductor substrate for biological analysis

Publications (2)

Publication Number Publication Date
WO2007141811A2 WO2007141811A2 (en) 2007-12-13
WO2007141811A3 true WO2007141811A3 (en) 2008-02-28

Family

ID=38801909

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IT2006/000420 WO2007141811A2 (en) 2006-06-06 2006-06-06 Process for preparing a semiconductor substrate for biological analysis

Country Status (1)

Country Link
WO (1) WO2007141811A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110081367A1 (en) 2008-06-14 2011-04-07 Veredus Laboratories Pte Ltd Influenza sequences

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990005910A1 (en) * 1988-11-14 1990-05-31 I Stat Corp Wholly microfabricated biosensors and process for the manufacture and use thereof
US5688642A (en) * 1994-12-01 1997-11-18 The United States Of America As Represented By The Secretary Of The Navy Selective attachment of nucleic acid molecules to patterned self-assembled surfaces
US5919523A (en) * 1995-04-27 1999-07-06 Affymetrix, Inc. Derivatization of solid supports and methods for oligomer synthesis
WO2001012846A1 (en) * 1999-08-16 2001-02-22 Pamgene B.V. Preparation of metal oxide supports loaded with biomolecules
US6284465B1 (en) * 1999-04-15 2001-09-04 Agilent Technologies, Inc. Apparatus, systems and method for locating nucleic acids bound to surfaces
US6387626B1 (en) * 1997-06-06 2002-05-14 Orchid Biosciences, Inc. Covalent attachment of unmodified nucleic acids to silanized solid phase surfaces
WO2003040700A1 (en) * 2001-11-08 2003-05-15 Ciphergen Biosystems, Inc. Hydrophobic surface chip
WO2004001506A2 (en) * 2002-06-20 2003-12-31 Affymetrix, Inc. Antireflective coatings for high-resolution photolithographic synthesis of dna array
EP1413352A1 (en) * 2002-10-21 2004-04-28 Agilent Technologies Inc. (a Delaware Corporation) Linking to chemical array assemblies with metal layers
WO2005005039A1 (en) * 2003-06-30 2005-01-20 Agilent Technologies, Inc. Methods for producing ligand arrays
WO2005029042A2 (en) * 2003-09-23 2005-03-31 Massachusetts Institute Of Technology Fabrication and packaging of suspended microchannel detectors

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990005910A1 (en) * 1988-11-14 1990-05-31 I Stat Corp Wholly microfabricated biosensors and process for the manufacture and use thereof
US5688642A (en) * 1994-12-01 1997-11-18 The United States Of America As Represented By The Secretary Of The Navy Selective attachment of nucleic acid molecules to patterned self-assembled surfaces
US5919523A (en) * 1995-04-27 1999-07-06 Affymetrix, Inc. Derivatization of solid supports and methods for oligomer synthesis
US6387626B1 (en) * 1997-06-06 2002-05-14 Orchid Biosciences, Inc. Covalent attachment of unmodified nucleic acids to silanized solid phase surfaces
US6284465B1 (en) * 1999-04-15 2001-09-04 Agilent Technologies, Inc. Apparatus, systems and method for locating nucleic acids bound to surfaces
WO2001012846A1 (en) * 1999-08-16 2001-02-22 Pamgene B.V. Preparation of metal oxide supports loaded with biomolecules
WO2003040700A1 (en) * 2001-11-08 2003-05-15 Ciphergen Biosystems, Inc. Hydrophobic surface chip
WO2004001506A2 (en) * 2002-06-20 2003-12-31 Affymetrix, Inc. Antireflective coatings for high-resolution photolithographic synthesis of dna array
EP1413352A1 (en) * 2002-10-21 2004-04-28 Agilent Technologies Inc. (a Delaware Corporation) Linking to chemical array assemblies with metal layers
WO2005005039A1 (en) * 2003-06-30 2005-01-20 Agilent Technologies, Inc. Methods for producing ligand arrays
WO2005029042A2 (en) * 2003-09-23 2005-03-31 Massachusetts Institute Of Technology Fabrication and packaging of suspended microchannel detectors

Also Published As

Publication number Publication date
WO2007141811A2 (en) 2007-12-13

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