WO2007140961A8 - Differential hemolysis of a whole blood sample - Google Patents

Differential hemolysis of a whole blood sample

Info

Publication number
WO2007140961A8
WO2007140961A8 PCT/EP2007/004923 EP2007004923W WO2007140961A8 WO 2007140961 A8 WO2007140961 A8 WO 2007140961A8 EP 2007004923 W EP2007004923 W EP 2007004923W WO 2007140961 A8 WO2007140961 A8 WO 2007140961A8
Authority
WO
WIPO (PCT)
Prior art keywords
red blood
blood cells
analyte
detecting
liquid sample
Prior art date
Application number
PCT/EP2007/004923
Other languages
French (fr)
Other versions
WO2007140961A1 (en
Inventor
Uwe Kobold
Thomas Duelffer
Rupert Herrmann
Der Eltz Herbert Von
Original Assignee
Roche Diagnostics Gmbh
Hoffmann La Roche
Uwe Kobold
Thomas Duelffer
Rupert Herrmann
Der Eltz Herbert Von
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh, Hoffmann La Roche, Uwe Kobold, Thomas Duelffer, Rupert Herrmann, Der Eltz Herbert Von filed Critical Roche Diagnostics Gmbh
Priority to CA2649633A priority Critical patent/CA2649633C/en
Priority to EP07725792.1A priority patent/EP2032979B1/en
Priority to CN2007800212732A priority patent/CN101467036B/en
Priority to ES07725792.1T priority patent/ES2690319T3/en
Priority to JP2009513584A priority patent/JP5156738B2/en
Publication of WO2007140961A1 publication Critical patent/WO2007140961A1/en
Publication of WO2007140961A8 publication Critical patent/WO2007140961A8/en
Priority to US12/328,890 priority patent/US7951598B2/en
Priority to HK09111529.8A priority patent/HK1134135A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5002—Partitioning blood components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces
    • G01N30/603—Construction of the column end pieces retaining the stationary phase, e.g. Frits
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation

Abstract

The invention relates to a method for differentially hemolyzing whole blood. It discloses method for detecting an analyte in a liquid sample known or suspected to comprise red blood cells and suspected or known to comprise eukaryotic cells, the method comprising the steps of processing said liquid sample with a membrane solubilizing agent under conditions appropriate to lyse cell membranes of red blood cells and at the same time not to cause precipitation of sample constituents, subjecting the processed sample to a chromatographic separation, and detecting the analyte. The differential hemolysis of red blood cells is of advantage in a method of detecting an analyte in a liquid sample that may comprise both erythrocytes as well as nucleated cells. The differential solubilization of red blood cells can be easily combined with an online detection methodology, like LC-MS, and is advantageous in the detection of many analytes, e.g. in the detection of folate or of immunosuppressive drugs, like tacrolimus or sirolimus.
PCT/EP2007/004923 2006-06-06 2007-06-04 Differential hemolysis of a whole blood sample WO2007140961A1 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CA2649633A CA2649633C (en) 2006-06-06 2007-06-04 Differential hemolysis of a whole blood sample
EP07725792.1A EP2032979B1 (en) 2006-06-06 2007-06-04 Differential hemolysis of a whole blood sample
CN2007800212732A CN101467036B (en) 2006-06-06 2007-06-04 Differential hemolysis of whole blood sample
ES07725792.1T ES2690319T3 (en) 2006-06-06 2007-06-04 Differential hemolysis of a whole blood sample
JP2009513584A JP5156738B2 (en) 2006-06-06 2007-06-04 Specific hemolysis of whole blood samples
US12/328,890 US7951598B2 (en) 2006-06-06 2008-12-05 Differential hemolysis of a whole blood sample
HK09111529.8A HK1134135A1 (en) 2006-06-06 2009-12-09 Differential hemolysis of a whole blood sample

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06011604.3 2006-06-06
EP06011604 2006-06-06

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/328,890 Continuation US7951598B2 (en) 2006-06-06 2008-12-05 Differential hemolysis of a whole blood sample

Publications (2)

Publication Number Publication Date
WO2007140961A1 WO2007140961A1 (en) 2007-12-13
WO2007140961A8 true WO2007140961A8 (en) 2008-11-06

Family

ID=37081679

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/004923 WO2007140961A1 (en) 2006-06-06 2007-06-04 Differential hemolysis of a whole blood sample

Country Status (8)

Country Link
US (1) US7951598B2 (en)
EP (1) EP2032979B1 (en)
JP (1) JP5156738B2 (en)
CN (1) CN101467036B (en)
CA (1) CA2649633C (en)
ES (1) ES2690319T3 (en)
HK (1) HK1134135A1 (en)
WO (1) WO2007140961A1 (en)

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JP5249905B2 (en) * 2009-10-29 2013-07-31 株式会社日立ハイテクノロジーズ Clinical laboratory system and clinical laboratory method
US20110217777A1 (en) * 2010-03-02 2011-09-08 Teixeira Rodrigo E Process for the Extraction of Lipids from Microalgae Using Ionic Liquids
US9063047B2 (en) * 2010-05-07 2015-06-23 Ut-Battelle, Llc System and method for extracting a sample from a surface
JP5452383B2 (en) * 2010-06-15 2014-03-26 株式会社日立ハイテクノロジーズ Biological sample pretreatment method and apparatus
CA2806261A1 (en) * 2010-08-11 2012-02-16 Kyowa Medex Co., Ltd. Method for measuring glycated hemoglobin
US8486703B2 (en) 2010-09-30 2013-07-16 Ut-Battelle, Llc Surface sampling concentration and reaction probe
US8519330B2 (en) 2010-10-01 2013-08-27 Ut-Battelle, Llc Systems and methods for laser assisted sample transfer to solution for chemical analysis
US8637813B2 (en) 2010-10-01 2014-01-28 Ut-Battelle, Llc System and method for laser assisted sample transfer to solution for chemical analysis
US8563298B2 (en) 2010-10-22 2013-10-22 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
ES2576927T3 (en) 2010-10-22 2016-07-12 T2 Biosystems, Inc. NMR systems and methods for rapid analyte detection
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WO2013021101A1 (en) 2011-08-08 2013-02-14 Thermo Fisher Scientific Oy Method and apparatus for automated analysis
FI20115785A0 (en) 2011-08-08 2011-08-08 Thermo Fisher Scientific Oy Method and apparatus for automatic analysis
EP2839038B1 (en) 2012-04-20 2019-01-16 T2 Biosystems, Inc. Compositions and methods for detection of candida species
CN108291896B (en) * 2015-12-18 2020-11-20 豪夫迈·罗氏有限公司 Automated clinical diagnostic system and method
EP3405479A4 (en) 2016-01-21 2019-08-21 T2 Biosystems, Inc. Nmr methods and systems for the rapid detection of bacteria
CN107561170B (en) * 2016-07-02 2021-07-30 山东新时代药业有限公司 Analysis and detection method of temsirolimus intermediate
CN106770747A (en) * 2016-12-09 2017-05-31 广州迈达康医药科技有限公司 A kind of tacrolimus formulations evaluation method
EP3592367A1 (en) * 2017-03-07 2020-01-15 Roche Diagnostics GmbH Stable formulations for immune-modulatory macrolides
CN113325171B (en) * 2021-08-02 2021-12-21 天津康博尔生物基因技术有限公司 Kit for detecting human body erythrocyte folic acid content, detection method and application
CN113776903A (en) * 2021-08-27 2021-12-10 深圳市希莱恒医用电子有限公司 Preparation method of pretreatment reagent, pretreatment reagent and pretreatment method

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Also Published As

Publication number Publication date
CA2649633A1 (en) 2007-12-13
US20090253210A1 (en) 2009-10-08
CN101467036A (en) 2009-06-24
CA2649633C (en) 2012-11-27
CN101467036B (en) 2013-06-19
HK1134135A1 (en) 2010-04-16
EP2032979B1 (en) 2018-07-18
JP5156738B2 (en) 2013-03-06
EP2032979A1 (en) 2009-03-11
US7951598B2 (en) 2011-05-31
WO2007140961A1 (en) 2007-12-13
ES2690319T3 (en) 2018-11-20
JP2009540274A (en) 2009-11-19

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