WO2007078439A3 - Electrofusion microelectrode and methods of using it to manipulate cells and/or cellular components - Google Patents

Electrofusion microelectrode and methods of using it to manipulate cells and/or cellular components Download PDF

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Publication number
WO2007078439A3
WO2007078439A3 PCT/US2006/044394 US2006044394W WO2007078439A3 WO 2007078439 A3 WO2007078439 A3 WO 2007078439A3 US 2006044394 W US2006044394 W US 2006044394W WO 2007078439 A3 WO2007078439 A3 WO 2007078439A3
Authority
WO
WIPO (PCT)
Prior art keywords
electrofusion
tube
methods
microelectrode
present
Prior art date
Application number
PCT/US2006/044394
Other languages
French (fr)
Other versions
WO2007078439A2 (en
Inventor
Gianpiero D Palermo
Original Assignee
Cornell Res Foundation Inc
Gianpiero D Palermo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cornell Res Foundation Inc, Gianpiero D Palermo filed Critical Cornell Res Foundation Inc
Priority to EP06837703A priority Critical patent/EP1963478A4/en
Priority to AU2006333433A priority patent/AU2006333433A1/en
Publication of WO2007078439A2 publication Critical patent/WO2007078439A2/en
Publication of WO2007078439A3 publication Critical patent/WO2007078439A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion

Abstract

The present invention relates to an electrofusion microelectrode made of a tube having a first proximal end and a second distal end. The tube has an electrically conductive coating on its exterior surface that extends continually from the first proximal end of the tube toward the second distal end of the tube. Also disclosed is an electrofusion microelectrode unit having an electrofusion microelectrode and a holding tool capable of receiving the electrofusion microelectrode at the second distal end of the tube. The present invention also relates to a system having two or more electrofusion microelectrodes of the present invention and to methods of manipulating cells and/or cellular components using the electrofusion microelectrodes, units, and systems of the present invention.
PCT/US2006/044394 2005-12-22 2006-11-14 Electrofusion microelectrode and methods of using it to manipulate cells and/or cellular components WO2007078439A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP06837703A EP1963478A4 (en) 2005-12-22 2006-11-14 Electrofusion microelectrode and methods of using it to manipulate cells and/or cellular components
AU2006333433A AU2006333433A1 (en) 2005-12-22 2006-11-14 Electrofusion microelectrode and methods of using it to manipulate cells and/or cellular components

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/315,475 US7915044B2 (en) 2005-12-22 2005-12-22 Electrofusion microelectrode and methods of using it to manipulate cells and/or cellular components
US11/315,475 2005-12-22

Publications (2)

Publication Number Publication Date
WO2007078439A2 WO2007078439A2 (en) 2007-07-12
WO2007078439A3 true WO2007078439A3 (en) 2009-04-30

Family

ID=38194318

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/044394 WO2007078439A2 (en) 2005-12-22 2006-11-14 Electrofusion microelectrode and methods of using it to manipulate cells and/or cellular components

Country Status (6)

Country Link
US (1) US7915044B2 (en)
EP (1) EP1963478A4 (en)
KR (1) KR20080082982A (en)
CN (1) CN101506346A (en)
AU (1) AU2006333433A1 (en)
WO (1) WO2007078439A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103382442B (en) * 2013-07-11 2015-03-18 湖北省农业科学院畜牧兽医研究所 Semi-fixed semi-mobile electrofusion electrode for mammal cloning
CN104075977A (en) * 2014-07-18 2014-10-01 苏州大学 Cell sucking, spitting and measuring device
CN108483389B (en) * 2018-03-09 2020-03-24 安徽师范大学 Silver nano electrode and preparation method thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4371273A (en) * 1981-01-16 1983-02-01 International Business Machines Corporation Electrochemical printhead

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US4441972A (en) 1983-04-08 1984-04-10 D.E.P. Systems, Inc. Apparatus for electrofusion of biological particles
US4923814A (en) 1986-05-09 1990-05-08 Electropore, Inc. High speed, high power apparatus for vesicle prealignment, poration, loading and fusion in uniform electric fields and method therefor
JPS6349070A (en) * 1986-08-19 1988-03-01 Shimadzu Corp Liquid flow-type cell fusion chamber
US5128257A (en) 1987-08-31 1992-07-07 Baer Bradford W Electroporation apparatus and process
US4970154A (en) 1987-10-09 1990-11-13 Baylor College Of Medicine Method for inserting foreign genes into cells using pulsed radiofrequency
US5007995A (en) 1989-05-11 1991-04-16 Olympus Optical Co., Ltd. Device for electrofusion of cells
US6001617A (en) 1989-06-07 1999-12-14 Queen's University At Kingston Electroporation device and method of use
US5185922A (en) 1990-08-17 1993-02-16 Cornell Research Foundation, Inc. Method of making submicrometer microelectrodes
JP3104995B2 (en) 1991-01-31 2000-10-30 ワーナー−ランバート・コンパニー Substituted heteroaryl analogs of 4,6-di-tert-butyl-5-hydroxy-1,3-pyrimidine useful as anti-inflammatory agents
US5304120A (en) 1992-07-01 1994-04-19 Btx Inc. Electroporation method and apparatus for insertion of drugs and genes into endothelial cells
US6009345A (en) 1992-08-17 1999-12-28 Genetronics, Inc. Method and apparatus for a combination of electroporation and iontophoresis for the delivery of drugs and genes
US5993434A (en) 1993-04-01 1999-11-30 Genetronics, Inc. Method of treatment using electroporation mediated delivery of drugs and genes
US5749837A (en) 1993-05-11 1998-05-12 Target Therapeutics, Inc. Enhanced lubricity guidewire
US5423812A (en) 1994-01-31 1995-06-13 Ellman; Alan G. Electrosurgical stripping electrode for palatopharynx tissue
AU5390696A (en) 1995-04-20 1996-11-07 University Of South Florida Purified and isolated sertoli cell aggregate
JPH08322548A (en) * 1995-05-31 1996-12-10 Nikon Corp Cell operation and apparatus therefor
US5709224A (en) 1995-06-07 1998-01-20 Radiotherapeutics Corporation Method and device for permanent vessel occlusion
US5983131A (en) 1995-08-11 1999-11-09 Massachusetts Institute Of Technology Apparatus and method for electroporation of tissue
US5859327A (en) 1995-08-22 1999-01-12 Genetronics, Inc. Electroporation-mediated molecular transfer in intact plants
US5650305A (en) 1996-05-15 1997-07-22 Health Research Inc. Method to produce hybrid cells by electrofusion
US5874268A (en) 1996-09-23 1999-02-23 Duke University Method of introducing exogenous compounds into cells by electroporation and apparatus for same
US6287831B1 (en) 1997-11-14 2001-09-11 California Institute Of Technology Cell lysis device
EP1079890A4 (en) 1998-05-08 2008-12-03 Genetronics Inc Electrically induced vessel vasodilation
US6355485B1 (en) 1998-06-10 2002-03-12 University Of South Florida Electrofusion chamber
US6150148A (en) 1998-10-21 2000-11-21 Genetronics, Inc. Electroporation apparatus for control of temperature during the process
US7133717B2 (en) 1999-08-25 2006-11-07 Johnson & Johnson Consumer Companies, Inc. Tissue electroperforation for enhanced drug delivery and diagnostic sampling
US6452564B1 (en) * 2001-03-09 2002-09-17 The United States Of America As Represented By The Secretary Of The Navy RF surface wave attenuating dielectric coatings composed of conducting, high aspect ratio biologically-derived particles in a polymer matrix
US7101703B2 (en) 2001-03-09 2006-09-05 Cornell Research Foundation, Inc. Electrofusion microelectrode

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4371273A (en) * 1981-01-16 1983-02-01 International Business Machines Corporation Electrochemical printhead

Also Published As

Publication number Publication date
EP1963478A2 (en) 2008-09-03
US7915044B2 (en) 2011-03-29
AU2006333433A1 (en) 2007-07-12
KR20080082982A (en) 2008-09-12
US20070148757A1 (en) 2007-06-28
EP1963478A4 (en) 2013-04-03
WO2007078439A2 (en) 2007-07-12
CN101506346A (en) 2009-08-12

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