WO2007024866A3 - Methods and compositions for the expression of a polynucleotide of interest - Google Patents

Methods and compositions for the expression of a polynucleotide of interest Download PDF

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Publication number
WO2007024866A3
WO2007024866A3 PCT/US2006/032792 US2006032792W WO2007024866A3 WO 2007024866 A3 WO2007024866 A3 WO 2007024866A3 US 2006032792 W US2006032792 W US 2006032792W WO 2007024866 A3 WO2007024866 A3 WO 2007024866A3
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WO
WIPO (PCT)
Prior art keywords
methods
polynucleotide
interest
compositions
seq
Prior art date
Application number
PCT/US2006/032792
Other languages
French (fr)
Other versions
WO2007024866A2 (en
Inventor
Billy Fred Mccutchen
Christine B Hazel
Donglong Liu
Albert L Lu
Wayne J Mehre
Paul D Olson
James F H Wong
Original Assignee
Pioneer Hi Bred Int
Du Pont
Billy Fred Mccutchen
Christine B Hazel
Donglong Liu
Albert L Lu
Wayne J Mehre
Paul D Olson
James F H Wong
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int, Du Pont, Billy Fred Mccutchen, Christine B Hazel, Donglong Liu, Albert L Lu, Wayne J Mehre, Paul D Olson, James F H Wong filed Critical Pioneer Hi Bred Int
Priority to AT06836107T priority Critical patent/ATE544861T1/en
Priority to EP06836107A priority patent/EP1931791B1/en
Priority to MX2008002615A priority patent/MX2008002615A/en
Priority to CA2620002A priority patent/CA2620002C/en
Priority to BRPI0615087-0A priority patent/BRPI0615087A2/en
Publication of WO2007024866A2 publication Critical patent/WO2007024866A2/en
Publication of WO2007024866A3 publication Critical patent/WO2007024866A3/en

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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
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Abstract

Methods and compositions for expressing a polynucleotide of interest are provided. Compositions comprise an enhancer domain set forth in SEQ ID NO: 1, 10, 15, or 16 and active variants and fragments thereof. Further provided are DNA constructs comprising at least one transcriptional enhancer sequence comprising the nucleotide sequence set forth in SEQ ID NO:1, 10, 15, or 16 or an active variant or fragment thereof, operably linked to a heterologous promoter. Such chimeric transcription regulatory regions can be operably linked any polynucleotide of interest. Further provided are cells, plants, plant parts, and germplasm comprising the DNA construct. Methods of using the chimeric transcriptional regulatory region are also provided. In specific embodiments, methods of expressing a polynucleotide of interest, including for example, sequences that confer tolerance to herbicides, and methods to select a cell having the DNA construct are provided.
PCT/US2006/032792 2005-08-24 2006-08-22 Methods and compositions for the expression of a polynucleotide of interest WO2007024866A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AT06836107T ATE544861T1 (en) 2005-08-24 2006-08-22 METHODS AND COMPOSITIONS FOR EXPRESSING A POLYNUCLEOTIDE OF INTEREST
EP06836107A EP1931791B1 (en) 2005-08-24 2006-08-22 Methods and compositions for the expression of a polynucleotide of interest
MX2008002615A MX2008002615A (en) 2005-08-24 2006-08-22 Methods and compositions for the expression of a polynucleotide of interest.
CA2620002A CA2620002C (en) 2005-08-24 2006-08-22 Methods and compositions for the expression of a polynucleotide of interest
BRPI0615087-0A BRPI0615087A2 (en) 2005-08-24 2006-08-22 methods and compositions for the expression of a polynucleotide of interest

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US71085405P 2005-08-24 2005-08-24
US60/710,854 2005-08-24
US81701106P 2006-06-28 2006-06-28
US60/817,011 2006-06-28

Publications (2)

Publication Number Publication Date
WO2007024866A2 WO2007024866A2 (en) 2007-03-01
WO2007024866A3 true WO2007024866A3 (en) 2007-06-21

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PCT/US2006/032792 WO2007024866A2 (en) 2005-08-24 2006-08-22 Methods and compositions for the expression of a polynucleotide of interest
PCT/US2006/032606 WO2007024782A2 (en) 2005-08-24 2006-08-22 Compositions providing tolerance to multiple herbicides and methods of use thereof

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PCT/US2006/032606 WO2007024782A2 (en) 2005-08-24 2006-08-22 Compositions providing tolerance to multiple herbicides and methods of use thereof

Country Status (22)

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US (8) US20070061917A1 (en)
EP (2) EP1917360A2 (en)
JP (1) JP2009505654A (en)
KR (1) KR20080052606A (en)
AP (1) AP2008004392A0 (en)
AR (2) AR055128A1 (en)
AT (1) ATE544861T1 (en)
AU (1) AU2006283504B2 (en)
BR (2) BRPI0615088A2 (en)
CA (2) CA2625371C (en)
CL (1) CL2009001635A1 (en)
CR (1) CR9757A (en)
EA (2) EA201000757A1 (en)
EC (1) ECSP088304A (en)
MA (1) MA29777B1 (en)
ME (1) MEP3508A (en)
MX (2) MX2008002616A (en)
NZ (1) NZ568867A (en)
RS (1) RS20080076A (en)
TW (1) TW200730625A (en)
UY (1) UY29761A1 (en)
WO (2) WO2007024866A2 (en)

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