WO2007011367A3 - Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16s ribosomal rna gene and identification of bacterial species by amplicon sequencing - Google Patents

Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16s ribosomal rna gene and identification of bacterial species by amplicon sequencing Download PDF

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Publication number
WO2007011367A3
WO2007011367A3 PCT/US2005/029296 US2005029296W WO2007011367A3 WO 2007011367 A3 WO2007011367 A3 WO 2007011367A3 US 2005029296 W US2005029296 W US 2005029296W WO 2007011367 A3 WO2007011367 A3 WO 2007011367A3
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Prior art keywords
bacteria
identification
chain reaction
polymerase chain
bacterial species
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PCT/US2005/029296
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French (fr)
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WO2007011367A2 (en
Inventor
Luc Montagnier
Claude Lavallee
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Luc Montagnier
Claude Lavallee
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Publication of WO2007011367A2 publication Critical patent/WO2007011367A2/en
Publication of WO2007011367A3 publication Critical patent/WO2007011367A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Abstract

A method for identifying an RNA form of a bacteria, comprising reverse transcribing RNA material; conducting PCR using primers for a first highly conserved genetic sequence generic of the bacteria; conducting nested PCR using primers for a second highly conserved genetic sequence within the first genetic sequence of the bacteria; and identifying the bacteria based on unconserved amplified sequences linked to the conserved sequences.
PCT/US2005/029296 2004-08-20 2005-08-17 Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16s ribosomal rna gene and identification of bacterial species by amplicon sequencing WO2007011367A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US60312004P 2004-08-20 2004-08-20
US60/603,120 2004-08-20
US11/204,854 US7309589B2 (en) 2004-08-20 2005-08-16 Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16S ribosomal RNA gene and identification of bacterial species by amplicon sequencing
US11/204,854 2005-08-16

Publications (2)

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WO2007011367A2 WO2007011367A2 (en) 2007-01-25
WO2007011367A3 true WO2007011367A3 (en) 2007-11-15

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WO (1) WO2007011367A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
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US7309589B2 (en) * 2004-08-20 2007-12-18 Vironix Llc Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16S ribosomal RNA gene and identification of bacterial species by amplicon sequencing
ES2342525B1 (en) * 2008-06-02 2011-04-26 Universidad De Vigo PROCEDURE FOR THE GENETIC IDENTIFICATION OF THE EUROPEAN CENTER OF THE ATLANTICO (MAJA BRACHYDACTYLA).
US8405379B1 (en) 2008-09-18 2013-03-26 Luc Montagnier System and method for the analysis of DNA sequences in biological fluids
ES2366289B2 (en) * 2010-03-18 2012-05-21 Universidade De Vigo PROCEDURE FOR THE GENETIC IDENTIFICATION OF THE EUROPEAN SPICES OF THE GENERO MAJA (CENTOLLAS).
MX2014000078A (en) * 2011-06-27 2014-05-01 Gen Mills Inc Detection and quantification of lactic acid producing bacteria in food products.
US10584370B2 (en) * 2014-12-16 2020-03-10 Soft Cell Biological Research, Llc Screening for L-form bacteria
US20220290212A1 (en) * 2019-08-28 2022-09-15 Contamination Source Identification Llc Rapid isolation and collection of microbial rna from a biological specimen
CN112941214B (en) * 2021-03-29 2023-04-11 中国农业大学 Primer group for gram-negative bacterium drug-resistant gene high-throughput amplicon sequencing and application
CN113564269A (en) * 2021-07-21 2021-10-29 翌圣生物科技(上海)股份有限公司 Probe composition for preventing reverse transcription of bacterial conserved region and application thereof

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