WO2007002567A3 - Selective isolation and concentration of nucleic acids from complex samples - Google Patents

Selective isolation and concentration of nucleic acids from complex samples Download PDF

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Publication number
WO2007002567A3
WO2007002567A3 PCT/US2006/024761 US2006024761W WO2007002567A3 WO 2007002567 A3 WO2007002567 A3 WO 2007002567A3 US 2006024761 W US2006024761 W US 2006024761W WO 2007002567 A3 WO2007002567 A3 WO 2007002567A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecules
concentration
nucleic acids
complex samples
Prior art date
Application number
PCT/US2006/024761
Other languages
French (fr)
Other versions
WO2007002567A2 (en
Inventor
Sudhakar S Marla
Anya Prokhorova
Susan Hetzel
William Cork
Original Assignee
Nanosphere Inc
Sudhakar S Marla
Anya Prokhorova
Susan Hetzel
William Cork
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanosphere Inc, Sudhakar S Marla, Anya Prokhorova, Susan Hetzel, William Cork filed Critical Nanosphere Inc
Publication of WO2007002567A2 publication Critical patent/WO2007002567A2/en
Publication of WO2007002567A3 publication Critical patent/WO2007002567A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Abstract

The present invention provides methods for detecting a target nucleic acid molecule in a sample that comprises nucleic acid molecules of higher biological complexity than that of amplified nucleic acid molecules in a complex environment containing numerous non-nucleic acid components. In particular, the present invention provides methods and probes for isolating DNA with detergents and detecting a single nucleotide polymorphism (SNP) in a complex sample that comprises numerous non-nucleic acid components and nucleic acid molecules of higher biological complexity than that of amplified nucleic acid molecules.
PCT/US2006/024761 2005-06-23 2006-06-23 Selective isolation and concentration of nucleic acids from complex samples WO2007002567A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69349105P 2005-06-23 2005-06-23
US60/693,491 2005-06-23

Publications (2)

Publication Number Publication Date
WO2007002567A2 WO2007002567A2 (en) 2007-01-04
WO2007002567A3 true WO2007002567A3 (en) 2007-03-29

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/024761 WO2007002567A2 (en) 2005-06-23 2006-06-23 Selective isolation and concentration of nucleic acids from complex samples

Country Status (2)

Country Link
US (1) US20070154903A1 (en)
WO (1) WO2007002567A2 (en)

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