WO2007002106A3 - Methods for the detection of colorectal cancer - Google Patents

Methods for the detection of colorectal cancer Download PDF

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Publication number
WO2007002106A3
WO2007002106A3 PCT/US2006/024006 US2006024006W WO2007002106A3 WO 2007002106 A3 WO2007002106 A3 WO 2007002106A3 US 2006024006 W US2006024006 W US 2006024006W WO 2007002106 A3 WO2007002106 A3 WO 2007002106A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene
methods
cell
detection
free protein
Prior art date
Application number
PCT/US2006/024006
Other languages
French (fr)
Other versions
WO2007002106A2 (en
Inventor
Sadanand Gite
Jennifer Mccullough
Kenneth J Rothschild
Original Assignee
Ambergen Inc
Sadanand Gite
Jennifer Mccullough
Kenneth J Rothschild
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ambergen Inc, Sadanand Gite, Jennifer Mccullough, Kenneth J Rothschild filed Critical Ambergen Inc
Priority to GB0724881A priority Critical patent/GB2443978A/en
Priority to US11/922,620 priority patent/US20110045991A1/en
Publication of WO2007002106A2 publication Critical patent/WO2007002106A2/en
Publication of WO2007002106A3 publication Critical patent/WO2007002106A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Abstract

This invention relates to an approach for detection of chain truncating mutations based on the utilization of existing sample collection methods such as FOBT platforms, together with advanced methods for cell-free protein expression. 'When further combined with mass spectrometry, the invention provides the ability to simultaneously detect changes in the amino acid sequence of multiple peptides. In some embodiments, DNA is isolated from a patient fecal sample and specific regions of a gene (i.e., for example, a K- ras gene or an APC gene) are PCR amplified using specifically designed primers that allow translation of encoded peptide fragments in a cell-free protein synthesis system. Nascent proteins are affinity purified and their mass is detected by MALDI-TOF which allows identifying low levels of mutations.
PCT/US2006/024006 2005-06-23 2006-06-21 Methods for the detection of colorectal cancer WO2007002106A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GB0724881A GB2443978A (en) 2005-06-23 2006-06-21 Methods for the detection of colorectal cancer
US11/922,620 US20110045991A1 (en) 2005-06-23 2006-06-21 Methods for the Detection of Colorectal Cancer

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/159,776 2005-06-23
US11/159,776 US8114587B2 (en) 2005-06-23 2005-06-23 Methods for the detection of colorectal cancer

Publications (2)

Publication Number Publication Date
WO2007002106A2 WO2007002106A2 (en) 2007-01-04
WO2007002106A3 true WO2007002106A3 (en) 2007-12-21

Family

ID=37590007

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/024006 WO2007002106A2 (en) 2005-06-23 2006-06-21 Methods for the detection of colorectal cancer

Country Status (3)

Country Link
US (1) US8114587B2 (en)
GB (1) GB2443978A (en)
WO (1) WO2007002106A2 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110045991A1 (en) * 2005-06-23 2011-02-24 Sadanand Gite Methods for the Detection of Colorectal Cancer
WO2007041213A2 (en) * 2005-09-30 2007-04-12 St. Jude Children's Research Hospital Methods for regulation of p53 translation and function
US20100304378A1 (en) * 2007-09-17 2010-12-02 Universite De Strasbourg Method for Detecting or Quantifying a Truncating Mutation
WO2010064628A1 (en) * 2008-12-05 2010-06-10 オリンパス株式会社 Method for preparing sample containing nucleic acid, solution for preparing sample, and method for analyzing nucleic acid
CA2741592A1 (en) * 2008-12-19 2010-06-24 Abbott Laboratories Diagnostic test for mutations in codons 12-13 of human k-ras
WO2010134245A1 (en) * 2009-05-20 2010-11-25 オリンパス株式会社 Method for collection of nucleic acid derived from mammalian cell, method for analysis of nucleic acid, and kit for collection of feces
US8722641B2 (en) 2010-01-29 2014-05-13 St. Jude Children's Research Hospital Oligonucleotides which inhibit p53 induction in response to cellular stress
WO2011126768A2 (en) * 2010-03-29 2011-10-13 Mayo Foundation For Medical Education And Research Methods and materials for detecting colorectal cancer and adenoma
US9938590B2 (en) * 2010-09-16 2018-04-10 Gen-Probe Incorporated Capture probes immobilizable via L-nucleotide tail
FR2970782A1 (en) * 2011-01-24 2012-07-27 Assist Publ Hopitaux De Paris MULTIFUNCTIONAL DIAGNOSTIC DEVICE
US20140316255A1 (en) * 2011-09-02 2014-10-23 Ellis Garai Raman Imaging Devices and Methods of Molecular Imaging
ES2712798T3 (en) 2013-03-14 2019-05-14 Exact Sciences Dev Co Llc Detection of neoplasms
EP4234722A3 (en) 2014-03-31 2023-09-20 Mayo Foundation for Medical Education and Research Detecting colorectal neoplasm
US10030272B2 (en) 2015-02-27 2018-07-24 Mayo Foundation For Medical Education And Research Detecting gastrointestinal neoplasms
KR20180081042A (en) 2015-08-31 2018-07-13 메이오 파운데이션 포 메디칼 에쥬케이션 앤드 리써치 A method for detecting gastric neoplasia
CN116024334A (en) 2016-04-14 2023-04-28 梅约医学教育与研究基金会 Application of detection DMR reagent in screening IPMN-HGD, panIN-3 or PDAC and kit
US10370726B2 (en) 2016-04-14 2019-08-06 Mayo Foundation For Medical Education And Research Detecting colorectal neoplasia
US10934592B2 (en) 2017-02-28 2021-03-02 Mayo Foundation For Medical Education And Research Detecting prostate cancer
WO2019108626A1 (en) 2017-11-30 2019-06-06 Mayo Foundation For Medical Education And Research Detecting breast cancer

Citations (1)

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US20060068400A1 (en) * 2004-09-30 2006-03-30 Cleator Iain G Device and method for fecal testing

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GITE ET AL.: "A high-throughput nonisotopic protein truncation test", NATURE BIOTECHNOLOGY, vol. 21, 2003, pages 194 - 197 *
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Also Published As

Publication number Publication date
GB0724881D0 (en) 2008-02-27
US8114587B2 (en) 2012-02-14
WO2007002106A2 (en) 2007-01-04
US20070003936A1 (en) 2007-01-04
GB2443978A (en) 2008-05-21

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