WO2006131892A3 - Amplification of nucleic acids with magnetic detection - Google Patents

Amplification of nucleic acids with magnetic detection Download PDF

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Publication number
WO2006131892A3
WO2006131892A3 PCT/IB2006/051817 IB2006051817W WO2006131892A3 WO 2006131892 A3 WO2006131892 A3 WO 2006131892A3 IB 2006051817 W IB2006051817 W IB 2006051817W WO 2006131892 A3 WO2006131892 A3 WO 2006131892A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
magnetic detection
amplification
detection
nucleic acid
Prior art date
Application number
PCT/IB2006/051817
Other languages
French (fr)
Other versions
WO2006131892A2 (en
Inventor
Menno W J Prins
Amerongen Aart Van
Maatje Koets
Original Assignee
Koninkl Philips Electronics Nv
Menno W J Prins
Amerongen Aart Van
Maatje Koets
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koninkl Philips Electronics Nv, Menno W J Prins, Amerongen Aart Van, Maatje Koets filed Critical Koninkl Philips Electronics Nv
Priority to EP06756080A priority Critical patent/EP1893774A2/en
Priority to US11/916,553 priority patent/US7863022B2/en
Priority to JP2008515366A priority patent/JP2008543279A/en
Publication of WO2006131892A2 publication Critical patent/WO2006131892A2/en
Publication of WO2006131892A3 publication Critical patent/WO2006131892A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors

Abstract

The present invention describes a method of amplifying nucleic acids and determining the amount of amplified nucleic acids using magnetic detection. The detection can be performed during the amplification process of the nucleic acid. During the detection, the amplified nucleic acid is bound to a sensor via a biological molecule.
PCT/IB2006/051817 2005-06-09 2006-06-07 Amplification of nucleic acids with magnetic detection WO2006131892A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP06756080A EP1893774A2 (en) 2005-06-09 2006-06-07 Amplification of nucleic acids with magnetic detection
US11/916,553 US7863022B2 (en) 2005-06-09 2006-06-07 Amplification of nucleic acids with magnetic detection
JP2008515366A JP2008543279A (en) 2005-06-09 2006-06-07 Nucleic acid amplification with magnetic detection

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP05105063 2005-06-09
EP05105063.1 2005-06-09
EP06113752 2006-05-10
EP06113752.7 2006-05-10

Publications (2)

Publication Number Publication Date
WO2006131892A2 WO2006131892A2 (en) 2006-12-14
WO2006131892A3 true WO2006131892A3 (en) 2007-02-22

Family

ID=37307100

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2006/051817 WO2006131892A2 (en) 2005-06-09 2006-06-07 Amplification of nucleic acids with magnetic detection

Country Status (4)

Country Link
US (1) US7863022B2 (en)
EP (1) EP1893774A2 (en)
JP (1) JP2008543279A (en)
WO (1) WO2006131892A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9599591B2 (en) 2009-03-06 2017-03-21 California Institute Of Technology Low cost, portable sensor for molecular assays

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2054526A2 (en) * 2006-08-14 2009-05-06 Koninklijke Philips Electronics N.V. Monitoring of enzymatic processes by using magnetizable or magnetic objects as labels
EP2077336A1 (en) * 2007-12-19 2009-07-08 Koninklijke Philips Electronics N.V. Device and method for parallel quantitative analysis of multiple nucleic acids
US20120040853A1 (en) * 2008-11-21 2012-02-16 Koninklijke Philips Electronics N.V. Real time multiplex pcr detection on solid surfaces using double stranded nucleic acid specific dyes
US8993236B2 (en) * 2009-04-17 2015-03-31 California Institute Of Technology Electromagnetic molecular sensors and methods of using same
CN102439448A (en) * 2009-05-19 2012-05-02 皇家飞利浦电子股份有限公司 Sensor device for magnetic particles with a high dynamic range
KR101107242B1 (en) * 2009-09-04 2012-01-25 동국대학교 산학협력단 Composition of magnetic particle and polymer having silicon group, heating element made from the composition, and biological or chemical reactions using thereof
GB201016484D0 (en) 2010-09-30 2010-11-17 Geneseque As Method
US9229071B2 (en) 2011-06-01 2016-01-05 International Business Machines Corporation Identification of molecules based on frequency responses using electromagnetic write-heads and magneto-resistive sensors
ITTO20120302A1 (en) 2012-04-05 2013-10-06 St Microelectronics Srl INTEGRATED DEVICE AND METHOD FOR CHAIN REACTION OF REAL-TIME QUANTITATIVE POLYMERASIS
US9512468B2 (en) * 2012-11-06 2016-12-06 Industrial Technology Research Institute Detection method uses magnetic and detectable nanoparticles with oligonucleotides attached thereto
GB201413929D0 (en) 2014-08-06 2014-09-17 Geneseque As Method
EP3184647A1 (en) * 2015-12-23 2017-06-28 Koninklijke Philips N.V. Laser pcr and bead detection in one reaction chamber
EP3290938A1 (en) 2016-09-05 2018-03-07 Industrial Technology Research Institute Biomolecule magnetic sensor

Citations (6)

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WO1996010644A1 (en) * 1994-10-03 1996-04-11 Adelman Lonnie W Magnetizable moieties in separation, sequencing and amplification of polynucleotides and polypeptides and magnetic detection thereof
WO1999007897A1 (en) * 1997-08-11 1999-02-18 Ericomp Magnetic polynucleotide separation and analysis
US20020006643A1 (en) * 1998-01-27 2002-01-17 Jon Faiz Kayyem Amplification of nucleic acids with electronic detection
US20020119470A1 (en) * 1999-04-13 2002-08-29 Nerenberg Michael I. Magnetic bead-based array for genetic detection
WO2003054523A2 (en) * 2001-12-21 2003-07-03 Koninklijke Philips Electronics N.V. Sensor and method for measuring the areal density of magnetic nanoparticles on a micro-array
WO2005007887A1 (en) * 2003-07-15 2005-01-27 Daniel Henry Densham Measurement of a polynucleotide amplification reaction

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CA2020958C (en) * 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5605662A (en) * 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
CA2381732A1 (en) 1999-08-21 2001-03-01 John S. Fox High sensitivity biomolecule detection with magnetic particles
US7682837B2 (en) 2000-05-05 2010-03-23 Board Of Trustees Of Leland Stanford Junior University Devices and methods to form a randomly ordered array of magnetic beads and uses thereof
US20030143604A1 (en) 2001-11-30 2003-07-31 Storhoff James J. Real-time monitoring of PCR amplification using nanoparticle probes
AUPS159702A0 (en) 2002-04-09 2002-05-16 Tong, Sun Wing Molecular detection and assay by magneto-thermal biochip micro-assay
US20060205093A1 (en) 2003-07-30 2006-09-14 Koninklijke Philips Electronics N.V. Use of magnetic particles for determining binding between bioactive molecules
CN101437614A (en) 2004-11-16 2009-05-20 皇家飞利浦电子股份有限公司 Microfluidic device

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996010644A1 (en) * 1994-10-03 1996-04-11 Adelman Lonnie W Magnetizable moieties in separation, sequencing and amplification of polynucleotides and polypeptides and magnetic detection thereof
WO1999007897A1 (en) * 1997-08-11 1999-02-18 Ericomp Magnetic polynucleotide separation and analysis
US20020006643A1 (en) * 1998-01-27 2002-01-17 Jon Faiz Kayyem Amplification of nucleic acids with electronic detection
US20020119470A1 (en) * 1999-04-13 2002-08-29 Nerenberg Michael I. Magnetic bead-based array for genetic detection
WO2003054523A2 (en) * 2001-12-21 2003-07-03 Koninklijke Philips Electronics N.V. Sensor and method for measuring the areal density of magnetic nanoparticles on a micro-array
WO2005007887A1 (en) * 2003-07-15 2005-01-27 Daniel Henry Densham Measurement of a polynucleotide amplification reaction

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9599591B2 (en) 2009-03-06 2017-03-21 California Institute Of Technology Low cost, portable sensor for molecular assays

Also Published As

Publication number Publication date
US20080207464A1 (en) 2008-08-28
EP1893774A2 (en) 2008-03-05
WO2006131892A2 (en) 2006-12-14
US7863022B2 (en) 2011-01-04
JP2008543279A (en) 2008-12-04

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