WO2006130790B1 - Counting, viability assessment, analysis and manipulation chamber - Google Patents

Counting, viability assessment, analysis and manipulation chamber

Info

Publication number
WO2006130790B1
WO2006130790B1 PCT/US2006/021329 US2006021329W WO2006130790B1 WO 2006130790 B1 WO2006130790 B1 WO 2006130790B1 US 2006021329 W US2006021329 W US 2006021329W WO 2006130790 B1 WO2006130790 B1 WO 2006130790B1
Authority
WO
WIPO (PCT)
Prior art keywords
cover
slide
fluid
overflow channel
viewing platform
Prior art date
Application number
PCT/US2006/021329
Other languages
French (fr)
Other versions
WO2006130790A1 (en
Inventor
Ludwig O Simmet
Original Assignee
Minitube America Inc
Ludwig O Simmet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Minitube America Inc, Ludwig O Simmet filed Critical Minitube America Inc
Priority to DE602006017206T priority Critical patent/DE602006017206D1/en
Priority to AT06771868T priority patent/ATE483179T1/en
Priority to EP06771868A priority patent/EP1886177B1/en
Publication of WO2006130790A1 publication Critical patent/WO2006130790A1/en
Publication of WO2006130790B1 publication Critical patent/WO2006130790B1/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/168—Specific optical properties, e.g. reflective coatings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1486—Counting the particles

Abstract

A transparent plastic slide (24) has a recessed chamber (26) which receives a transparent plastic cover (28). The cover has a fluid inlet opening (62) which overlies a recessed center well (42) in the slide. The well has a sloping ramp (66) which extends to a viewing platform (48) which has a surface which is closely spaced from the underside of the cover to define a constant thickness viewing cavity surrounding the well. An overflow channel (44) surrounds the viewing platform. A vent hole (72) extends through the cover above the overflow channel. The sample is introduced into the center well through the fluid inlet opening. The fluid flows into the viewing cavities by capillary action, and is free to overflow the viewing cavities and be received within the overflow channel, thus reducing boundary effects. An inlet port may be provided above the viewing platform to permit substances to be added to a sample while it is under observation.

Claims

AMENDED CLAIMS received by the International Bureau on 30 November 2006 (30.11.2006)CLAIMS
1. An apparatus for observing a fluid, the apparatus comprising: a transparent slide having an upper surface defining a recessed center well and a recessed overflow channel, the well spaced apart from the overflow channel with portions of the slide therebetween defining a viewing platform having a surface; and a transparent cover having a lower surface overlying the slide upper surface and spaced from the surface of the viewing platform to define a capillary flow channel in fluid communication with the well and the overflow channel, and a fluid inlet opening extending through the cover for passage of fluid into the center well of the slide.
2. The apparatus of Claim 1, further comprising at least one vent hole extending through the cover in communication with the overflow channel.
3. The apparatus of Claim 1, wherein the viewing platform encircles the well, and wherein the overflow channel encircles the viewing platform.
4. The apparatus of Claim 1, further comprising a plurality of ribs which extend downwardly from the cover and which engage the viewing platform surface of the slide, the ribs being of a constant thickness which thereby defines the constant thickness of the fluid on the viewing platform.
5. The apparatus of Claim 1, wherein the slide comprises at least one projection which engages with a positioning notch situated on the cover to position the cover over the slide.
6. The apparatus of Claim 5, further comprising adhesive disposed between the at least one projection and the positioning notch to secure the cover to the slide.
7. The apparatus of Claim 1, wherein an unobscured viewing region is defined to overlie the viewing platform, and further comprising an obscuring surface treatment on a portion of the cover in regions surrounding the unobscured viewing region.
8. The apparatus of Claim 1, wherein the cover is spaced from the viewing platform a distance of approximately 12 microns.
9. The apparatus of Claim 1, wherein the cover is spaced from the viewing platform a distance of approximately 20 microns.
10. The apparatus of Claim 1, wherein the well has a central region which is depressed below the viewing platform, and a continuous sloped lead-in ramp extends between the central region and the viewing platform.
11. The apparatus of Claim 1, wherein the slide and the cover comprise a plastic material.
12. The apparatus of Claim 11, wherein the plastic material comprises a cyclic olefin copolymer.
13. The apparatus of Claim 11 , wherein the plastic material comprises polycarbonate.
14. The apparatus of Claim 1 , further comprising a secondary inlet port extending through the cover and in fluid commtmication with the viewing platform for passage of a fluid therethrough.
15. The apparatus of Claim 1, wherein the viewing platform surface is recessed below the level of the upper surface of the slide.
16. The apparatus of Claim 1, further comprising portions of the slide which define a relieved region beneath the viewing cavity, the underside of the slide within the relieved region being spaced above the underside of the slide exterior to the relieved region.
17. The apparatus of Claim 1, wherein the cover is fixed to the slide.
18. The apparatus of Claim 17, wherein the lower surface of the cover defines a recessed overflow channel which is positioned to overlie the overflow channel in the upper surface of the slide.
19. A transparent cover for positioning on a slide to observe a fluid situated thereon, the cover comprising: a cover body having an upper surface and an opposing lower surface with portions of the lower surface being parallel to an observation region on the upper surface, a sample inlet opening and a vent hole extending through the cover body with the observation region positioned therebetween, and at least one rib protruding downwardly from the lower surface.
20. The transparent cover of Claim 19, wherein the lower surface of the cover body defines a recessed overflow channel in communication with the vent hole.
21. The transparent cover of Claim 19 or 20, being mounted on a transparent slide and spaced apart to define a capillary flow channel between the lower surface of the cover body and the slide in fluid communication with the sample inlet opening and the vent hole.
22. An apparatus for observing a fluid, the apparatus comprising: a transparent slide having an upper surface; and a transparent cover having an upper surface having an observation region, an opposing lower surface overlying and spaced apart from the upper surface of the slide, portions of the lower surface of the cover being parallel to said observation region, the lower surface of the cover defining a recessed overflow channel, a sample inlet opening extending through the cover body and spaced apart from the overflow channel with the observation region positioned therebetween, a vent hole extending through the cover body in communication with the overflow channel, and at least one rib protruding downwardly from the lower surface; the lower surface of the cover spaced apart from the upper surface of the slide to define a capillary flow channel in fluid communication with the sample inlet opening and the overflow channel.
23. The apparatus of Claim 22, wherein the upper surface of the slide cover defines a recessed overflow channel which is positioned adjacent to and in communication with the overflow channel in the lower surface of the cover.
24. The apparatus of Claim 22, wherein the cover further defines a secondary inlet port extending therethrough for passage of a fluid into the capillary flow channel.
25. A process for observing a fluid situated on a slide, comprising the steps of: providing the apparatus of Claim 1; introducing the fluid through the fluid inlet opening in the cover and into the recessed center well in the slide such that the fluid passes into the capillary flow channel and the overflow channel with a defined thickness of fluid over the surface of the viewing platform of the slide; and observing the fluid situated on the viewing platform under magnification.
26. The process of Claim 25, further comprising the step of adding an additive substance to the fluid through an opening in the cover.
27. The process of Claim 26, wherein the additive substance is introduced through a secondary inlet port positioned above the viewing platform.
28. The process of Claim 26, wherein the additive substance comprises a culture medium.
29. The process of Claim 26, wherein the additive substance comprises a pharmaceutical substance.
30. The process of claim 25, wherein the fluid comprises sperm cells, and the observing step comprises counting the sperm cells visible through a defined region of the cover.
31. The process of Claim 25, further comprising the step of adding an additive substance to the sample through an opening in the cover; observing the sperm cells visible through the said defined region of the cover; and recording an effect of the additive substance on the sperm cells.
32. The process of Claim 25, wherein the apparatus further comprises at least one rib extending downwardly from the cover and engaging the viewing platform surface of the slide, the rib comprising a narrow closed ring of side wall defining an interior volume, and the cover is fixed to the slide; and the process further comprises the step of observing the interior volume of the rib through the cover to detect the presence of the fluid therein and confirm the proper assembly of the apparatus.
33. A process for observing a fluid situated on a slide, comprising the steps of: providing the apparatus of Claim 22; introducing the fluid through the sample inlel opening in the cover and onto the slide such thai the fluid passes into the capillary flow channel and the overflow channel with a defined thickness of fluid within the observation region; and observing the fluid situated within the observation region under magnification.
PCT/US2006/021329 2005-06-02 2006-06-01 Counting, viability assessment, analysis and manipulation chamber WO2006130790A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
DE602006017206T DE602006017206D1 (en) 2005-06-02 2006-06-01 CHAMBER FOR COUNTING, ASSESSMENT OF LIFE, ANALYSIS AND MANIPULATION
AT06771868T ATE483179T1 (en) 2005-06-02 2006-06-01 CHAMBER FOR COUNTING, VIABILITY ASSESSMENT, ANALYSIS AND MANIPULATION
EP06771868A EP1886177B1 (en) 2005-06-02 2006-06-01 Counting, viability assessment, analysis and manipulation chamber

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/142,929 2005-06-02
US11/142,929 US7718124B2 (en) 2005-06-02 2005-06-02 Counting, viability assessment, analysis and manipulation chamber

Publications (2)

Publication Number Publication Date
WO2006130790A1 WO2006130790A1 (en) 2006-12-07
WO2006130790B1 true WO2006130790B1 (en) 2007-01-25

Family

ID=36968495

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/021329 WO2006130790A1 (en) 2005-06-02 2006-06-01 Counting, viability assessment, analysis and manipulation chamber

Country Status (6)

Country Link
US (1) US7718124B2 (en)
EP (2) EP1886177B1 (en)
AT (1) ATE483179T1 (en)
DE (1) DE602006017206D1 (en)
ES (1) ES2504241T3 (en)
WO (1) WO2006130790A1 (en)

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US20150031071A1 (en) * 2012-03-01 2015-01-29 Victorious Medical Systems Aps Method and System for Distributing and Agitating an Amount of Liquid Over a Microscope Slide
JP2013061357A (en) * 2013-01-08 2013-04-04 Shimadzu Corp Sample cell and particle size distribution measurement apparatus using the same
BR102014028937A2 (en) 2013-11-19 2015-10-13 Univ Toronto sperm separation apparatus and methods
CN105793694A (en) * 2013-12-12 2016-07-20 梅斯医疗电子系统有限公司 Home testing device
IL301722B1 (en) * 2016-04-22 2024-02-01 Protein Dynamic Solutions Inc Sampling array devices and system for spectral analysis
EP3504567B1 (en) * 2016-08-26 2023-08-09 Molecular Imprints, Inc. Edge sealant confinement and halo reduction for optical devices
US11513372B2 (en) 2018-06-12 2022-11-29 Magic Leap, Inc. Edge sealant application for optical devices
WO2020081672A1 (en) 2018-10-16 2020-04-23 Magic Leap, Inc. Methods and apparatuses for casting polymer products
WO2021035044A1 (en) * 2019-08-20 2021-02-25 Pattern Bioscience, Inc. Microfluidic chips including a gutter to facilitate loading thereof and related methods
CN110567961A (en) * 2019-10-11 2019-12-13 长沙傲图生物科技有限公司 Semen detection device

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Also Published As

Publication number Publication date
EP1886177B1 (en) 2010-09-29
EP2270573A1 (en) 2011-01-05
US7718124B2 (en) 2010-05-18
ATE483179T1 (en) 2010-10-15
ES2504241T3 (en) 2014-10-08
US20060275743A1 (en) 2006-12-07
EP1886177A1 (en) 2008-02-13
DE602006017206D1 (en) 2010-11-11
EP2270573B1 (en) 2014-08-13
WO2006130790A1 (en) 2006-12-07

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