WO2006106331A3 - Mouse primers - Google Patents

Mouse primers Download PDF

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Publication number
WO2006106331A3
WO2006106331A3 PCT/GB2006/001251 GB2006001251W WO2006106331A3 WO 2006106331 A3 WO2006106331 A3 WO 2006106331A3 GB 2006001251 W GB2006001251 W GB 2006001251W WO 2006106331 A3 WO2006106331 A3 WO 2006106331A3
Authority
WO
WIPO (PCT)
Prior art keywords
development
mouse primers
antibodies
mouse
primers
Prior art date
Application number
PCT/GB2006/001251
Other languages
French (fr)
Other versions
WO2006106331A2 (en
Inventor
Ralph Adams
Andrew George Popplewell
Patrick Marcel Slocombe
Original Assignee
Ucb Sa
Ralph Adams
Andrew George Popplewell
Patrick Marcel Slocombe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ucb Sa, Ralph Adams, Andrew George Popplewell, Patrick Marcel Slocombe filed Critical Ucb Sa
Publication of WO2006106331A2 publication Critical patent/WO2006106331A2/en
Publication of WO2006106331A3 publication Critical patent/WO2006106331A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Abstract

The present invention provides primer sets for the isolation of mouse antibody variable region sequences. Further provided are antibodies comprising these regions which are useful in the development of therapeutics and, in particular, in the development of humanised antibodies.
PCT/GB2006/001251 2005-04-06 2006-04-05 Mouse primers WO2006106331A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0507046A GB0507046D0 (en) 2005-04-06 2005-04-06 Primers
GB0507046.1 2005-04-06

Publications (2)

Publication Number Publication Date
WO2006106331A2 WO2006106331A2 (en) 2006-10-12
WO2006106331A3 true WO2006106331A3 (en) 2007-03-29

Family

ID=34586839

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2006/001251 WO2006106331A2 (en) 2005-04-06 2006-04-05 Mouse primers

Country Status (2)

Country Link
GB (1) GB0507046D0 (en)
WO (1) WO2006106331A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0675199A2 (en) * 1994-03-30 1995-10-04 Takara Shuzo Co. Ltd. A DNA which codes for the variable region of the anti-human influenza A type virus antibody recognizing the H,N, and H2N2 subtypes of haemagglutinin
WO2000023087A1 (en) * 1998-10-21 2000-04-27 Sunol Molecular Corporation Polyspecific binding molecules and uses thereof
US6291161B1 (en) * 1989-05-16 2001-09-18 Scripps Research Institute Method for tapping the immunological repertiore
WO2003054016A2 (en) * 2001-12-21 2003-07-03 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Method for cloning of variable domain sequences

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6291161B1 (en) * 1989-05-16 2001-09-18 Scripps Research Institute Method for tapping the immunological repertiore
EP0675199A2 (en) * 1994-03-30 1995-10-04 Takara Shuzo Co. Ltd. A DNA which codes for the variable region of the anti-human influenza A type virus antibody recognizing the H,N, and H2N2 subtypes of haemagglutinin
WO2000023087A1 (en) * 1998-10-21 2000-04-27 Sunol Molecular Corporation Polyspecific binding molecules and uses thereof
WO2003054016A2 (en) * 2001-12-21 2003-07-03 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Method for cloning of variable domain sequences

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
"PRIMER DESIGN FOR THE CLONING OF IMMUNOGLOBULIN HEAVY-CHAIN LEADER-VARIABLE REGIONS FROM MOUSE HYBRIDOMA CELLS USING THE PCR", BIOTECHNIQUES, INFORMA LIFE SCIENCES PUBLISHING, WESTBOROUGH, MA, US, vol. 11, no. 2, August 1991 (1991-08-01), pages 152 - 154,156, XP000647450, ISSN: 0736-6205 *
BABCOOK J S ET AL: "A NOVEL STRATEGY FOR GENERATING MONOCLONAL ANTIBODIES FROM SINGLE, ISOLATED LYMPHOCYTES PRODUCING ANTIBODIES OF DEFINED SPECIFICITIES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 93, no. 15, 23 July 1996 (1996-07-23), pages 7843 - 7848, XP000608647, ISSN: 0027-8424 *
DATABASE Geneseq [online] 15 August 2000 (2000-08-15), "F23.1 heavy chain PCR primer JS300.", XP002393358, retrieved from EBI accession no. GSN:AAZ94969 Database accession no. AAZ94969 *
DATABASE Geneseq [online] 9 May 1996 (1996-05-09), "Primer for mouse heavy chain constant region.", XP002393359, retrieved from EBI accession no. GSN:AAT04162 Database accession no. AAT04162 *
DÜBEL S ET AL: "Isolation of IgG antibody Fv-DNA from various mouse and rat hybridoma cell lines using the polymerase chain reaction with a simple set of primers.", JOURNAL OF IMMUNOLOGICAL METHODS. 30 SEP 1994, vol. 175, no. 1, 30 September 1994 (1994-09-30), pages 89 - 95, XP002079907, ISSN: 0022-1759 *
FORD JOHN E ET AL: "Chimeric molecules created by gene amplification interfere with the analysis of somatic hypermutation of murine immunoglobulin genes", GENE (AMSTERDAM), vol. 142, no. 2, 1994, pages 279 - 283, XP002393354, ISSN: 0378-1119 *
GAVILONDO-COWLEY J V ET AL: "SPECIFIC AMPLIFICATION OF REARRANGED IMMUNOGLOBULIN VARIABLE REGION GENES FROM MOUSE HYBRIDOMA CELLS", HYBRIDOMA, LIEBERT, NEW YORK, NY, US, vol. 407 - 417, no. 9, January 1990 (1990-01-01), pages 5, XP008067250, ISSN: 0272-457X *
MAYNARD J ET AL: "ANTIBODY ENGINEERING", ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, ANNUAL REVIEW INCO., PALO ALTO, CA, US, vol. 2, 2000, pages 339 - 376, XP009039750 *
ORLANDI R ET AL: "CLONING IMMUNOGLOBULIN VARIABLE DOMAINS FOR EXPRESSION BY THE POLYMERASE CHAIN REACTION", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 86, no. 10, 1 May 1989 (1989-05-01), pages 3833 - 3837, XP000026475, ISSN: 0027-8424 *
ORUM H ET AL: "Efficient method for constructing comprehensive murine Fab antibody libraries displayed on phage.", NUCLEIC ACIDS RESEARCH. 25 SEP 1993, vol. 21, no. 19, 25 September 1993 (1993-09-25), pages 4491 - 4498, XP002393352, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
GB0507046D0 (en) 2005-05-11
WO2006106331A2 (en) 2006-10-12

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