WO2006102352A3 - Normalization of data using controls - Google Patents

Normalization of data using controls Download PDF

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Publication number
WO2006102352A3
WO2006102352A3 PCT/US2006/010291 US2006010291W WO2006102352A3 WO 2006102352 A3 WO2006102352 A3 WO 2006102352A3 US 2006010291 W US2006010291 W US 2006010291W WO 2006102352 A3 WO2006102352 A3 WO 2006102352A3
Authority
WO
WIPO (PCT)
Prior art keywords
data
normalization
controls
assay
sample
Prior art date
Application number
PCT/US2006/010291
Other languages
French (fr)
Other versions
WO2006102352A2 (en
Inventor
H Pin Kao
Ian A Harding
Achim Karger
Mark F Oldham
Omead Ostadan
Greg Young
Kathleen C Hayashibara
Hyland Fiona C Laird
Mark R Pratt
Junco F Stevens
Austin B Tomaney
Original Assignee
Applera Corp
H Pin Kao
Ian A Harding
Achim Karger
Mark F Oldham
Omead Ostadan
Greg Young
Kathleen C Hayashibara
Hyland Fiona C Laird
Mark R Pratt
Junco F Stevens
Austin B Tomaney
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/087,103 external-priority patent/US7417726B2/en
Priority claimed from US11/086,260 external-priority patent/US20060024690A1/en
Application filed by Applera Corp, H Pin Kao, Ian A Harding, Achim Karger, Mark F Oldham, Omead Ostadan, Greg Young, Kathleen C Hayashibara, Hyland Fiona C Laird, Mark R Pratt, Junco F Stevens, Austin B Tomaney filed Critical Applera Corp
Publication of WO2006102352A2 publication Critical patent/WO2006102352A2/en
Publication of WO2006102352A3 publication Critical patent/WO2006102352A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

A method of using a standard to correct for variability in sample handling, can comprise (a) adding a template of known concentration to an assay comprising a sample; (b) preamplifying the assay; (c) amplifying the assay; (d) collecting data during the amplifying; and (e) correcting the data using a comparison of data collected from the template to data collected from the sample.
PCT/US2006/010291 2005-03-22 2006-03-21 Normalization of data using controls WO2006102352A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US11/086,260 2005-03-22
US11/087,103 US7417726B2 (en) 2003-09-19 2005-03-22 Normalization of data using controls
US11/087,103 2005-03-22
US11/086,260 US20060024690A1 (en) 2003-09-19 2005-03-22 Normalization of data using controls

Publications (2)

Publication Number Publication Date
WO2006102352A2 WO2006102352A2 (en) 2006-09-28
WO2006102352A3 true WO2006102352A3 (en) 2007-07-19

Family

ID=37024539

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/010291 WO2006102352A2 (en) 2005-03-22 2006-03-21 Normalization of data using controls

Country Status (1)

Country Link
WO (1) WO2006102352A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170212050A1 (en) * 2016-01-22 2017-07-27 Ionfield Holdings, Llc Method for calibrating and using calibration of microplates
US11686208B2 (en) 2020-02-06 2023-06-27 Rolls-Royce Corporation Abrasive coating for high-temperature mechanical systems
CN114248294B (en) * 2021-12-23 2023-06-13 北京电子科技职业学院 Dust-resistant testing device for industrial robot in high-temperature environment

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002052038A2 (en) * 2000-12-27 2002-07-04 Geneka Biotechnology Inc. Method for normalizing the relative intensities of detection signals in hybridization arrays
US6471916B1 (en) * 1999-11-09 2002-10-29 Packard Instrument Company Apparatus and method for calibration of a microarray scanning system

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6471916B1 (en) * 1999-11-09 2002-10-29 Packard Instrument Company Apparatus and method for calibration of a microarray scanning system
WO2002052038A2 (en) * 2000-12-27 2002-07-04 Geneka Biotechnology Inc. Method for normalizing the relative intensities of detection signals in hybridization arrays

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YANG M.C.K. ET AL.: "A statistical method for flagging weak spots improves normalization and ratio estimates in microarrays", PHYSICAL GENOMICS, vol. 7, August 2001 (2001-08-01), pages 45 - 53, XP002971145 *

Also Published As

Publication number Publication date
WO2006102352A2 (en) 2006-09-28

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