WO2006086209A3 - Genetic analysis by sequence-specific sorting - Google Patents

Genetic analysis by sequence-specific sorting Download PDF

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Publication number
WO2006086209A3
WO2006086209A3 PCT/US2006/003688 US2006003688W WO2006086209A3 WO 2006086209 A3 WO2006086209 A3 WO 2006086209A3 US 2006003688 W US2006003688 W US 2006003688W WO 2006086209 A3 WO2006086209 A3 WO 2006086209A3
Authority
WO
WIPO (PCT)
Prior art keywords
polynucleotides
subpopulation
sequence characteristics
predetermined sequence
sequence
Prior art date
Application number
PCT/US2006/003688
Other languages
French (fr)
Other versions
WO2006086209A2 (en
Inventor
Sydney Brenner
Original Assignee
Compass Genetics Llc
Sydney Brenner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/055,187 external-priority patent/US7217522B2/en
Priority claimed from US11/173,465 external-priority patent/US7407757B2/en
Priority claimed from US11/176,927 external-priority patent/US7393665B2/en
Application filed by Compass Genetics Llc, Sydney Brenner filed Critical Compass Genetics Llc
Publication of WO2006086209A2 publication Critical patent/WO2006086209A2/en
Publication of WO2006086209A3 publication Critical patent/WO2006086209A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Abstract

The invention provides methods for sorting polynucleotides from a population based on predetermined sequence characteristics. In one aspect, the method of the invention is carried out by extending a primer annealed polynucleotides having predetermined sequence characteristics to incorporate a predetermined terminator having a capture moiety, capturing polynucleotides having extended primers by a capture agent that specifically binds to the capture moiety, and melting the captured polynucleotides from the extended primers to form a subpopulation of polynucleotides having the predetermined sequence characteristics. In another aspect, the method of the invention is carried out on a population of tagged polynucleotides so that after a subpopulation is selected, the members of the subpopulation may be simultaneously analyzed using the unique tags on the polynucleotides to convey analytical information to a hybridization array for a readout.
PCT/US2006/003688 2005-02-10 2006-02-03 Genetic analysis by sequence-specific sorting WO2006086209A2 (en)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US11/055,187 US7217522B2 (en) 2004-02-12 2005-02-10 Genetic analysis by sequence-specific sorting
US11/055,187 2005-02-10
US66216705P 2005-03-16 2005-03-16
US60/662,167 2005-03-16
US11/173,465 2005-06-30
US11/173,465 US7407757B2 (en) 2005-02-10 2005-06-30 Genetic analysis by sequence-specific sorting
US11/176,927 US7393665B2 (en) 2005-02-10 2005-07-07 Methods and compositions for tagging and identifying polynucleotides
US11/176,927 2005-07-07

Publications (2)

Publication Number Publication Date
WO2006086209A2 WO2006086209A2 (en) 2006-08-17
WO2006086209A3 true WO2006086209A3 (en) 2007-09-13

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/003688 WO2006086209A2 (en) 2005-02-10 2006-02-03 Genetic analysis by sequence-specific sorting

Country Status (1)

Country Link
WO (1) WO2006086209A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11845054B2 (en) 2010-11-12 2023-12-19 Gen9, Inc. Methods and devices for nucleic acids synthesis

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
WO2009031054A2 (en) * 2007-06-29 2009-03-12 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
DK3594340T3 (en) 2011-08-26 2021-09-20 Gen9 Inc COMPOSITIONS AND METHODS FOR COLLECTING WITH HIGH ACCURACY OF NUCLEIC ACIDS
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
EP3543350B1 (en) 2012-04-24 2021-11-10 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
IL236303B (en) 2012-06-25 2022-07-01 Gen9 Inc Methods for nucleic acid assembly and high throughput sequencing

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030143580A1 (en) * 2001-09-06 2003-07-31 Don Straus Rapid and sensitive detection of molecules
US20030207300A1 (en) * 2000-04-28 2003-11-06 Matray Tracy J. Multiplex analytical platform using molecular tags
US20040259118A1 (en) * 2003-06-23 2004-12-23 Macevicz Stephen C. Methods and compositions for nucleic acid sequence analysis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030207300A1 (en) * 2000-04-28 2003-11-06 Matray Tracy J. Multiplex analytical platform using molecular tags
US20030143580A1 (en) * 2001-09-06 2003-07-31 Don Straus Rapid and sensitive detection of molecules
US20040259118A1 (en) * 2003-06-23 2004-12-23 Macevicz Stephen C. Methods and compositions for nucleic acid sequence analysis

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11845054B2 (en) 2010-11-12 2023-12-19 Gen9, Inc. Methods and devices for nucleic acids synthesis

Also Published As

Publication number Publication date
WO2006086209A2 (en) 2006-08-17

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