WO2006084068A3 - Method of adjusting the working range of a multi-analyte assay - Google Patents
Method of adjusting the working range of a multi-analyte assay Download PDFInfo
- Publication number
- WO2006084068A3 WO2006084068A3 PCT/US2006/003737 US2006003737W WO2006084068A3 WO 2006084068 A3 WO2006084068 A3 WO 2006084068A3 US 2006003737 W US2006003737 W US 2006003737W WO 2006084068 A3 WO2006084068 A3 WO 2006084068A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- analyte
- scaling
- concentration
- assay
- high end
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
Abstract
The invention features a method of adjusting the concentration of at least one but not all of a plurality of analytes in a fluid sample to match a known working range of detection of an analyte assay system, where each of the plurality of analytes may or may not be present within an expected initial concentration range having a high end and a low end, and at least one analyte has a high end expected concentration range that exceeds the high end of the working range of the assay system. The expected concentration of the high concentration analyte is adjusted by a proportional scaling constant, α, so that the high end of the adjusted expected concentration range is less than or equal to the high end of the working range, without adjusting the expected concentration range of at least one other of the plurality of analytes. Adjustment is preferably accomplished by adding to the solution phase of the assay one or more scaling agents, each scaling agent binding with specificity to an analyte and thereby preventing it from being detected by the assay system, e.g., by competing with binding to immobilized capture agent. This scaling method contrasts with prior methods, in which a concentration of available analyte is offset by a fixed amount to adjust the detectable threshold of the assay. Here, the amount of scaling agent is proportional to a scaling coefficient and the scaling agent is present in the solution phase of the assay at high concentrations relative to analyte. Due to the equilibrium conditions established by the laws of mass transfer, the amount of free analyte remaining in solution in the presence of scaling agent is predictable and finite, and can be measured as a quantitative indicator of the initial concentration of the analyte in the sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP06720171A EP1848998A4 (en) | 2005-02-04 | 2006-02-03 | Method of adjusting the working range of a multi-analyte assay |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US65032405P | 2005-02-04 | 2005-02-04 | |
| US60/650,324 | 2005-02-04 | ||
| US11/221,198 US7396689B2 (en) | 2005-02-04 | 2005-09-06 | Method of adjusting the working range of a multi-analyte assay |
| US11/221,198 | 2005-09-06 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2006084068A2 WO2006084068A2 (en) | 2006-08-10 |
| WO2006084068A3 true WO2006084068A3 (en) | 2007-01-11 |
Family
ID=36777929
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2006/003737 WO2006084068A2 (en) | 2005-02-04 | 2006-02-03 | Method of adjusting the working range of a multi-analyte assay |
Country Status (3)
| Country | Link |
|---|---|
| US (2) | US7396689B2 (en) |
| EP (1) | EP1848998A4 (en) |
| WO (1) | WO2006084068A2 (en) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8481334B1 (en) | 2001-11-06 | 2013-07-09 | Charm Sciences, Inc. | Method of attaching a ligand to a solid support |
| EP1519194A1 (en) * | 2003-09-24 | 2005-03-30 | Roche Diagnostics GmbH | Use of gfap for identification of intracerebral hemorrhage |
| US20060151704A1 (en) * | 2004-12-30 | 2006-07-13 | Cordingley James J | Laser-based material processing methods, system and subsystem for use therein for precision energy control |
| US7396689B2 (en) * | 2005-02-04 | 2008-07-08 | Decision Biomarkers Incorporated | Method of adjusting the working range of a multi-analyte assay |
| US20060264783A1 (en) | 2005-05-09 | 2006-11-23 | Holmes Elizabeth A | Systems and methods for monitoring pharmacological parameters |
| US11287421B2 (en) | 2006-03-24 | 2022-03-29 | Labrador Diagnostics Llc | Systems and methods of sample processing and fluid control in a fluidic system |
| US8741230B2 (en) | 2006-03-24 | 2014-06-03 | Theranos, Inc. | Systems and methods of sample processing and fluid control in a fluidic system |
| US8007999B2 (en) | 2006-05-10 | 2011-08-30 | Theranos, Inc. | Real-time detection of influenza virus |
| US20090047673A1 (en) | 2006-08-22 | 2009-02-19 | Cary Robert B | Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids |
| DE502006005987D1 (en) * | 2006-10-20 | 2010-03-11 | Micronas Holding Gmbh | Method and apparatus for determining the concentrations of ligands |
| US20080113391A1 (en) | 2006-11-14 | 2008-05-15 | Ian Gibbons | Detection and quantification of analytes in bodily fluids |
| US8158430B1 (en) | 2007-08-06 | 2012-04-17 | Theranos, Inc. | Systems and methods of fluidic sample processing |
| WO2009137059A1 (en) | 2008-05-05 | 2009-11-12 | Los Alamos National Security, Llc | Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control |
| US9927435B2 (en) * | 2009-10-15 | 2018-03-27 | Robert Bosch Gmbh | Multisite biosensor and associated method |
| BR112012009196B1 (en) | 2009-10-19 | 2021-03-30 | Labrador Diagnostics Llc | SYSTEM FOR MODELING THE PROGRESSION OF A DISEASE WITHIN A POPULATION |
| CA2806306A1 (en) * | 2010-07-23 | 2012-01-26 | Luminex Corporation | Co-coupling to control reactivity of reagents in immunoassays |
| US8956859B1 (en) | 2010-08-13 | 2015-02-17 | Aviex Technologies Llc | Compositions and methods for determining successful immunization by one or more vaccines |
| PT2699698T (en) | 2011-04-20 | 2017-04-11 | Mesa Biotech Inc | Oscillating amplification reaction for nucleic acids |
| US20140308751A1 (en) * | 2013-04-12 | 2014-10-16 | Siemens Healthcare Diagnostics Inc. | Assays for Analyte Homologs |
| WO2015006503A1 (en) * | 2013-07-09 | 2015-01-15 | Smith Lucas David | Device and method of rapid linker mediated label-based immunoassays |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4743542A (en) * | 1985-04-11 | 1988-05-10 | Ortho Diagnostic | Method for forestalling the hook effect in a multi-ligand immunoassay system |
| US20030004571A1 (en) * | 1999-12-01 | 2003-01-02 | Pace Danny R. | External breast prosthesis support |
| US6551788B1 (en) * | 2001-11-28 | 2003-04-22 | Beckman Coulter, Inc. | Particle-based ligand assay with extended dynamic range |
| US20030078737A1 (en) * | 2001-10-24 | 2003-04-24 | Keys Daniel A. | Method and apparatus for increasing the dynamic range and accuracy of binding assays |
| US20050112585A1 (en) * | 2003-11-21 | 2005-05-26 | Dominic Zichi | Method for adjusting the quantification range of individual analytes in a multiplexed assay |
Family Cites Families (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL7807532A (en) * | 1978-07-13 | 1980-01-15 | Akzo Nv | METAL IMMUNO TEST. |
| US4517289A (en) * | 1982-08-18 | 1985-05-14 | Brigham And Women's Hospital | Monoclonal antibodies for human tissue cross-matching |
| CA1339723C (en) | 1988-01-19 | 1998-03-17 | Philip Mcmahon | Immunoassay with multiple test spots |
| US5939272A (en) | 1989-01-10 | 1999-08-17 | Biosite Diagnostics Incorporated | Non-competitive threshold ligand-receptor assays |
| US5028535A (en) | 1989-01-10 | 1991-07-02 | Biosite Diagnostics, Inc. | Threshold ligand-receptor assay |
| CA2072758A1 (en) | 1990-09-14 | 1992-03-15 | Kenneth Francis Buechler | Antibodies to complexes of ligand receptors and ligands and their utility in ligand-receptor assays |
| WO1992012428A1 (en) | 1991-01-11 | 1992-07-23 | Quidel Corporation | A one-step lateral flow nonbibulous assay |
| JPH04351962A (en) | 1991-05-29 | 1992-12-07 | Mochida Pharmaceut Co Ltd | Analysis of specific combination and device thereof |
| US5468648A (en) | 1991-05-29 | 1995-11-21 | Smithkline Diagnostics, Inc. | Interrupted-flow assay device |
| US5877028A (en) | 1991-05-29 | 1999-03-02 | Smithkline Diagnostics, Inc. | Immunochromatographic assay device |
| US5607863A (en) | 1991-05-29 | 1997-03-04 | Smithkline Diagnostics, Inc. | Barrier-controlled assay device |
| US5869345A (en) | 1991-05-29 | 1999-02-09 | Smithkline Diagnostics, Inc. | Opposable-element assay device employing conductive barrier |
| EP0525723B1 (en) | 1991-07-29 | 1997-05-14 | Mochida Pharmaceutical Co., Ltd. | Process and device for specific binding assay |
| WO1993015230A1 (en) | 1992-01-22 | 1993-08-05 | Abbott Laboratories | Calibration reagents for semi-quantitative binding assays and devices |
| US5541069A (en) | 1992-02-28 | 1996-07-30 | Quidel Corporation | Assay having improved dose response curve |
| JP3299330B2 (en) | 1993-03-18 | 2002-07-08 | 持田製薬株式会社 | Simple measuring device and method |
| AU693442B2 (en) | 1993-03-30 | 1998-07-02 | Telik, Inc. | Determination of concentration by affinity titration and competitive displacement in drug delivery |
| US5356784A (en) | 1993-03-30 | 1994-10-18 | Terrapin Technologies, Inc. | Determination of concentration by affinity titration |
| JP3479100B2 (en) | 1993-06-02 | 2003-12-15 | 帝国臓器製薬株式会社 | Simple semi-quantitative immunochemical method and apparatus |
| US5756362A (en) | 1993-10-12 | 1998-05-26 | Cornell Research Foundation, Inc. | Liposome-enhanced immunoaggregation assay and test device |
| EP0690306A1 (en) | 1994-06-28 | 1996-01-03 | Mochida Pharmaceutical Co., Ltd. | Method and device for specific binding assay |
| US5521102A (en) | 1994-08-08 | 1996-05-28 | Quidel Corporation | Controlled sensitivity immunochromatographic assay |
| GB2300914B (en) | 1995-04-28 | 1998-04-29 | Tepnel Medical Ltd | Analytical device |
| AU6720096A (en) | 1995-08-09 | 1997-03-05 | Quidel Corporation | Test strip and method for one step lateral flow assay |
| US5874216A (en) | 1996-02-23 | 1999-02-23 | Ensys Environmental Products, Inc. | Indirect label assay device for detecting small molecules and method of use thereof |
| EP0890104B1 (en) | 1996-03-29 | 2001-08-01 | University Of British Columbia | Platelet count assay using platelet granule proteins |
| US5968839A (en) | 1996-05-13 | 1999-10-19 | Metrika, Inc. | Method and device producing a predetermined distribution of detectable change in assays |
| DE19621133A1 (en) * | 1996-05-24 | 1997-11-27 | Boehringer Mannheim Gmbh | Determination method with oligomerized receptors |
| DE19629444A1 (en) | 1996-07-22 | 1998-01-29 | Behringwerke Ag | Increased sensitivity in the immunochemical determination of an analyte |
| US5710005A (en) | 1996-10-29 | 1998-01-20 | Biocode, Inc. | Analyte detection with a gradient lateral flow device |
| US5879951A (en) | 1997-01-29 | 1999-03-09 | Smithkline Diagnostics, Inc. | Opposable-element assay device employing unidirectional flow |
| CZ297165B6 (en) | 1997-04-21 | 2006-09-13 | Randox Laboratories Ltd. A British Company Of Ardmore | Solid state device for performing multi-analyte assays |
| US5939252A (en) | 1997-05-09 | 1999-08-17 | Lennon; Donald J. | Detachable-element assay device |
| US6670196B1 (en) | 1997-05-14 | 2003-12-30 | Biosite, Inc. | Rapid evaluation of the ratio of biological molecules |
| US5985675A (en) | 1997-12-31 | 1999-11-16 | Charm Sciences, Inc. | Test device for detection of an analyte |
| US6319466B1 (en) | 1997-07-16 | 2001-11-20 | Charm Sciences, Inc. | Test device for detecting the presence of a residue analyte in a sample |
| US6180340B1 (en) * | 1997-10-31 | 2001-01-30 | Gen-Probe Incorporated | Extended dynamic range assays |
| US6406921B1 (en) * | 1998-07-14 | 2002-06-18 | Zyomyx, Incorporated | Protein arrays for high-throughput screening |
| US6410341B1 (en) | 1998-08-06 | 2002-06-25 | Spectral Diagnostics, Inc. | Analytical test device and method for use in medical diagnoses |
| US6284472B1 (en) | 1998-10-05 | 2001-09-04 | Dade Behring Inc. | Method for extending the range of an immunoassay |
| US6136610A (en) | 1998-11-23 | 2000-10-24 | Praxsys Biosystems, Inc. | Method and apparatus for performing a lateral flow assay |
| US6680209B1 (en) | 1999-12-06 | 2004-01-20 | Biosite, Incorporated | Human antibodies as diagnostic reagents |
| US6509196B1 (en) | 2000-01-04 | 2003-01-21 | Response Biomedical Corp. | Compensation for non-specific signals in quantitative immunoassays |
| US6316205B1 (en) | 2000-01-28 | 2001-11-13 | Genelabs Diagnostics Pte Ltd. | Assay devices and methods of analyte detection |
| AU2001251413B2 (en) | 2000-04-13 | 2004-07-29 | Bio-Rad Laboratories, Inc. | Multi-analyte diagnostic test for thyroid disorders |
| GB0027064D0 (en) * | 2000-11-06 | 2000-12-20 | Randox Lab Ltd | Multi-analyte immunoassay |
| US6696243B2 (en) | 2001-01-23 | 2004-02-24 | Coulter International Corp. | Method for the analysis of soluble analytes |
| US20060084068A1 (en) | 2004-10-07 | 2006-04-20 | Johnson Mark K | Process for detecting a nucleic acid target |
| US7396689B2 (en) * | 2005-02-04 | 2008-07-08 | Decision Biomarkers Incorporated | Method of adjusting the working range of a multi-analyte assay |
-
2005
- 2005-09-06 US US11/221,198 patent/US7396689B2/en not_active Expired - Fee Related
-
2006
- 2006-02-03 EP EP06720171A patent/EP1848998A4/en not_active Withdrawn
- 2006-02-03 WO PCT/US2006/003737 patent/WO2006084068A2/en active Application Filing
-
2008
- 2008-07-03 US US12/217,423 patent/US20090061535A1/en not_active Abandoned
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4743542A (en) * | 1985-04-11 | 1988-05-10 | Ortho Diagnostic | Method for forestalling the hook effect in a multi-ligand immunoassay system |
| US20030004571A1 (en) * | 1999-12-01 | 2003-01-02 | Pace Danny R. | External breast prosthesis support |
| US20030078737A1 (en) * | 2001-10-24 | 2003-04-24 | Keys Daniel A. | Method and apparatus for increasing the dynamic range and accuracy of binding assays |
| US6551788B1 (en) * | 2001-11-28 | 2003-04-22 | Beckman Coulter, Inc. | Particle-based ligand assay with extended dynamic range |
| US20050112585A1 (en) * | 2003-11-21 | 2005-05-26 | Dominic Zichi | Method for adjusting the quantification range of individual analytes in a multiplexed assay |
Also Published As
| Publication number | Publication date |
|---|---|
| EP1848998A4 (en) | 2008-10-01 |
| US7396689B2 (en) | 2008-07-08 |
| EP1848998A2 (en) | 2007-10-31 |
| US20090061535A1 (en) | 2009-03-05 |
| US20060177873A1 (en) | 2006-08-10 |
| WO2006084068A2 (en) | 2006-08-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2006084068A3 (en) | Method of adjusting the working range of a multi-analyte assay | |
| WO2006047182A3 (en) | Method and apparatus for determining an analyte concentration in a sample having interferents | |
| WO2010065781A3 (en) | Lateral flow strip assay with immobilized conjugate | |
| WO2008008785A3 (en) | Ultra-sensitive detection of analytes | |
| WO2006079049A3 (en) | Methods and compositions for increased dynamic range detection of nucleic acid molecules | |
| WO2007092627A3 (en) | Detection of cancer by elevated levels of bcl-2 | |
| WO2005106036A3 (en) | Methods and compositions for assaying analytes | |
| MX355017B (en) | Multiplexed analyses of test samples. | |
| AU1038402A (en) | Assay for directly detecting a rs virus related biological cell in a body fluid sample | |
| WO2008051316A3 (en) | Nanosensors and related technologies | |
| WO2008066607A3 (en) | Device for detection of molecules in biological fluids | |
| WO2008070865A3 (en) | Materials and methods for efficient and accurate detection of analytes | |
| WO2009066731A1 (en) | Measurement reagent, immune nephelometry using the same, and analyte analysis tool | |
| WO2007005666A3 (en) | System and method of analyte detection using differential receptors | |
| BRPI0811536A8 (en) | sucinylacetone detection | |
| WO2007061940A3 (en) | Method for detecting an inflammatory disease or cancer | |
| DE60225802D1 (en) | METHOD AND APPARATUS FOR DETECTING THE WHOLE PROTEIN CONTENT IN LOW PH VALUES | |
| WO2019113075A3 (en) | Microbial-based biosensors | |
| Yildirim et al. | Aptamer based E-coli detection in waste waters by portable optical biosensor system | |
| WO2007092552A3 (en) | Device and methods for detecting and quantifying one or more target agents | |
| WO2007076013A3 (en) | Homocysteine immunoassay | |
| WO2008067008A3 (en) | A method for the quantitative evaluation of sex hormones in a serum sample | |
| WO2020264444A3 (en) | Assay method for point of care quantification of an immunophilin-binding immunosuppressant drug | |
| WO2007061656A3 (en) | Method for detecting ovarian cancer | |
| WO2005074522A3 (en) | Detection of ruminant dna via pcr |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
| NENP | Non-entry into the national phase |
Ref country code: DE |
|
| WWE | Wipo information: entry into national phase |
Ref document number: 2006720171 Country of ref document: EP |