WO2006082058A3 - Cell-free translation system for the production of (chemically) modified proteins - Google Patents

Cell-free translation system for the production of (chemically) modified proteins Download PDF

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Publication number
WO2006082058A3
WO2006082058A3 PCT/EP2006/000926 EP2006000926W WO2006082058A3 WO 2006082058 A3 WO2006082058 A3 WO 2006082058A3 EP 2006000926 W EP2006000926 W EP 2006000926W WO 2006082058 A3 WO2006082058 A3 WO 2006082058A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
translation system
relates
free translation
release factor
Prior art date
Application number
PCT/EP2006/000926
Other languages
French (fr)
Other versions
WO2006082058A2 (en
Inventor
Mathias Sprinzl
Dmitry Agafonov
Kersten Rabe
Original Assignee
Univ Bayreuth
Mathias Sprinzl
Dmitry Agafonov
Kersten Rabe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Bayreuth, Mathias Sprinzl, Dmitry Agafonov, Kersten Rabe filed Critical Univ Bayreuth
Publication of WO2006082058A2 publication Critical patent/WO2006082058A2/en
Publication of WO2006082058A3 publication Critical patent/WO2006082058A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1203—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria

Abstract

The present invention relates to a ceil-free translation system comprising a nonsense-codon suppressing agent and an anti-release factor antibody which precipitates an/ or crosslinks a release factor in said cell-free translation system. The present invention further relates to a kit comprising said cell free translation system. Moreover, the present invention relates to an anti-release factor antibody directed against release factor of Thermus thermophiles. Furthermore, the present invention relates to the use of an anti-release factor antibody for the preparation of a celi-free translation system. Moreover, the present invention relates to a method for the production of an anti-release factor antibody. Furthermore, the present invention provides for a method for the production of alloproteins. Further, the present invention relates to a puromycin derivative having a residue covalently attached directly or via a linker to one of its elements selected from the group consisting of the element N4 of the cytosine-residue of an 51 attached cytidine-residue and the element C5 of the cytosine-residue of an 5' attached cytidine-residue. Moreover, the present invention relates to the use of said puromycin derivative in the cell-free translation system of the present invention, in the method for the production of alloproteins of the present invention. The invention also provides for specific kits described herein.
PCT/EP2006/000926 2005-02-02 2006-02-02 Cell-free translation system for the production of (chemically) modified proteins WO2006082058A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05002201.1 2005-02-02
EP05002201 2005-02-02

Publications (2)

Publication Number Publication Date
WO2006082058A2 WO2006082058A2 (en) 2006-08-10
WO2006082058A3 true WO2006082058A3 (en) 2006-11-09

Family

ID=36570537

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2006/000926 WO2006082058A2 (en) 2005-02-02 2006-02-02 Cell-free translation system for the production of (chemically) modified proteins

Country Status (1)

Country Link
WO (1) WO2006082058A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2997705B1 (en) * 2012-11-08 2015-10-16 Nicolas Ugolin NUCLEOTIDE 3 'BLOCKING TRANSLATION OF PROTEINS (SASB)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4828979A (en) * 1984-11-08 1989-05-09 Life Technologies, Inc. Nucleotide analogs for nucleic acid labeling and detection
US5405950A (en) * 1985-05-15 1995-04-11 Amoco Corporation N4 -substituted cytidines and process for incorporation into an oligonucleotides
EP1350846A1 (en) * 2000-12-07 2003-10-08 Keio University C-terminal modified protein and process for producing the same, modifying agent and translation template to be used in produfing c-terminal modified protein, and method of detecting protein interaction with the use of c-termial modified protein

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4828979A (en) * 1984-11-08 1989-05-09 Life Technologies, Inc. Nucleotide analogs for nucleic acid labeling and detection
US5405950A (en) * 1985-05-15 1995-04-11 Amoco Corporation N4 -substituted cytidines and process for incorporation into an oligonucleotides
EP1350846A1 (en) * 2000-12-07 2003-10-08 Keio University C-terminal modified protein and process for producing the same, modifying agent and translation template to be used in produfing c-terminal modified protein, and method of detecting protein interaction with the use of c-termial modified protein

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
AGAFONOV D E ET AL: "C-terminal modifications of a protein by UAG-encoded incorporation of puromycin during in vitro protein synthesis in the absence of release factor 1", CHEMBIOCHEM 2006 GERMANY, vol. 7, no. 2, 2006, pages 330 - 336, XP002385501, ISSN: 1439-4227 1439-7633 *
AGAFONOV D E ET AL: "Efficient suppression of the amber codon in E. coli in vitro translation system", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 579, no. 10, 11 April 2005 (2005-04-11), pages 2156 - 2160, XP004850720, ISSN: 0014-5793 *
DOI NOBUHIDE ET AL: "Novel fluorescence labeling and high-throughput assay technologies for in vitro analysis of protein interactions", GENOME RESEARCH, vol. 12, no. 3, March 2002 (2002-03-01), pages 487 - 492, XP002394791, ISSN: 1088-9051 *
MIYAMOTO-SATO E ET AL: "SPECIFIC BONDING OF PUROMYCIN TO FULL-LENGTH PROTEIN AT THE C-TERMINUS", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 28, no. 5, 2000, pages 1176 - 1182, XP002908838, ISSN: 0305-1048 *
SHIMIZU Y ET AL: "CELL-FREE TRANSLATION RECONSTITUTED WITH PURIFIED COMPONENTS", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 19, August 2001 (2001-08-01), pages 751 - 755, XP001060378, ISSN: 1087-0156 *
SHORT GLENN F III ET AL: "Effects of release factor 1 on in vitro protein translation and the elaboration of proteins containing unnatural amino acids", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY, EASTON, PA, US, vol. 38, no. 27, 6 July 1999 (1999-07-06), pages 8808 - 8819, XP002195367, ISSN: 0006-2960 *
SZKARADKIEWICZ K ET AL: "RNA aptamers directed against release factor 1 from Thermus thermophilus", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 514, no. 1, 6 March 2002 (2002-03-06), pages 90 - 95, XP004597956, ISSN: 0014-5793 *

Also Published As

Publication number Publication date
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