WO2006081583A3 - Methods and compositions for improving fidelity in a nucleic acid synthesis reaction - Google Patents

Methods and compositions for improving fidelity in a nucleic acid synthesis reaction Download PDF

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Publication number
WO2006081583A3
WO2006081583A3 PCT/US2006/004638 US2006004638W WO2006081583A3 WO 2006081583 A3 WO2006081583 A3 WO 2006081583A3 US 2006004638 W US2006004638 W US 2006004638W WO 2006081583 A3 WO2006081583 A3 WO 2006081583A3
Authority
WO
WIPO (PCT)
Prior art keywords
compositions
synthesis reaction
methods
nucleic acid
acid synthesis
Prior art date
Application number
PCT/US2006/004638
Other languages
French (fr)
Other versions
WO2006081583A2 (en
Inventor
Philip Richard Buzby
Original Assignee
Helicos Biosciences Corp
Philip Richard Buzby
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Helicos Biosciences Corp, Philip Richard Buzby filed Critical Helicos Biosciences Corp
Priority to CA002599477A priority Critical patent/CA2599477A1/en
Priority to AT06734692T priority patent/ATE548466T1/en
Priority to EP06734692A priority patent/EP1848829B1/en
Publication of WO2006081583A2 publication Critical patent/WO2006081583A2/en
Publication of WO2006081583A3 publication Critical patent/WO2006081583A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Abstract

The invention provides methods and compositions for improving the fidelity of a sequencing-by-synthesis reaction by using a nucleotide derivative that forms a hydrogen bond with a complementary nucleotide on a template, but fails to form a phosphodiester bond with the 3' hydroxyl group of a primer under conditions otherwise suitable for a polymerization reaction; thereby blocking incorporation of a mismatched nucleotide.
PCT/US2006/004638 2005-01-28 2006-01-30 Methods and compositions for improving fidelity in a nucleic acid synthesis reaction WO2006081583A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002599477A CA2599477A1 (en) 2005-01-28 2006-01-30 Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
AT06734692T ATE548466T1 (en) 2005-01-28 2006-01-30 METHODS AND COMPOSITIONS FOR IMPROVING THE ACCURACY OF A NUCLEIC ACID SYNTHESIS REACTION
EP06734692A EP1848829B1 (en) 2005-01-28 2006-01-30 Methods and compositions for improving fidelity in a nucleic acid synthesis reaction

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/046,448 US7482120B2 (en) 2005-01-28 2005-01-28 Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US11/046,448 2005-01-28

Publications (2)

Publication Number Publication Date
WO2006081583A2 WO2006081583A2 (en) 2006-08-03
WO2006081583A3 true WO2006081583A3 (en) 2006-10-19

Family

ID=36582013

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/004638 WO2006081583A2 (en) 2005-01-28 2006-01-30 Methods and compositions for improving fidelity in a nucleic acid synthesis reaction

Country Status (5)

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US (1) US7482120B2 (en)
EP (1) EP1848829B1 (en)
AT (1) ATE548466T1 (en)
CA (1) CA2599477A1 (en)
WO (1) WO2006081583A2 (en)

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