WO2006071568A3 - Methods, compositions, and kits for forming labeled polynucleotides - Google Patents

Methods, compositions, and kits for forming labeled polynucleotides Download PDF

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Publication number
WO2006071568A3
WO2006071568A3 PCT/US2005/045615 US2005045615W WO2006071568A3 WO 2006071568 A3 WO2006071568 A3 WO 2006071568A3 US 2005045615 W US2005045615 W US 2005045615W WO 2006071568 A3 WO2006071568 A3 WO 2006071568A3
Authority
WO
WIPO (PCT)
Prior art keywords
labeled
labeled polynucleotides
kits
compositions
forming
Prior art date
Application number
PCT/US2005/045615
Other languages
French (fr)
Other versions
WO2006071568A2 (en
Inventor
Lori K Hennessy
Mark R Andersen
Original Assignee
Applera Corp
Lori K Hennessy
Mark R Andersen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp, Lori K Hennessy, Mark R Andersen filed Critical Applera Corp
Publication of WO2006071568A2 publication Critical patent/WO2006071568A2/en
Publication of WO2006071568A3 publication Critical patent/WO2006071568A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

The present teachings provide novel methods, compositions, and kits for forming multi-labeled polynucleotides. In some embodiments of the present teachings, multiplexed amplification reactions are performed with a plurality of primer pairs, wherein one primer in a given primer pair comprises a distinct label. Additional labeling of the resulting amplicons can be accomplished by using at least one bridge oligonucleotide to ligate a labeled tag oligonucleotide to each labeled extension product, thereby forming a plurality of multi-labeled polynucleotides. Detection of labels such as florophores and mobility modifiers in the plurality of multi-labeled polynucleotides can identify a sample. Such sample identification can be performed using a mobility dependent analysis technique such as capillary electrophoresis, and can applicable in the field of forensics.
PCT/US2005/045615 2004-12-29 2005-12-15 Methods, compositions, and kits for forming labeled polynucleotides WO2006071568A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US64012704P 2004-12-29 2004-12-29
US60/640,127 2004-12-29

Publications (2)

Publication Number Publication Date
WO2006071568A2 WO2006071568A2 (en) 2006-07-06
WO2006071568A3 true WO2006071568A3 (en) 2006-10-05

Family

ID=36423625

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/045615 WO2006071568A2 (en) 2004-12-29 2005-12-15 Methods, compositions, and kits for forming labeled polynucleotides

Country Status (2)

Country Link
US (1) US20060166235A1 (en)
WO (1) WO2006071568A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7862999B2 (en) 2007-01-17 2011-01-04 Affymetrix, Inc. Multiplex targeted amplification using flap nuclease
US8685899B2 (en) 2007-02-14 2014-04-01 Genisphere Inc. Methods, reagents and kits for detection of nucleic acid molecules
US20100190167A1 (en) * 2007-02-14 2010-07-29 Genisphere Inc. Methods, Reagents and Kits for Detection of Nucleic Acid Molecules
US20080293589A1 (en) * 2007-05-24 2008-11-27 Affymetrix, Inc. Multiplex locus specific amplification
CN101144774B (en) * 2007-08-24 2010-05-19 张兹钧 Human STRtyper PCR amplification fluorescence detection reagent kit
US20090233809A1 (en) * 2008-03-04 2009-09-17 Affymetrix, Inc. Resequencing methods for identification of sequence variants
EP2113574A1 (en) 2008-04-28 2009-11-04 Biotype AG Substances and methods for a DNA based profiling assay
CN104031989B (en) * 2014-05-16 2016-01-20 无锡中德美联生物技术有限公司 The test kit of the composite amplification of a kind of human gene group DNA 26 locus
CN105018623B (en) * 2015-08-06 2018-04-20 无锡中德美联生物技术有限公司 Fluorescence labeling composite amplification kit and its application of huamn autosomal and Y chromosome str locus seat are expanded at the same time
US9382582B1 (en) * 2015-08-25 2016-07-05 Tracy Hayden Methods, compositions and kits for enriching for a minor template amplification product in a mixed template amplification reaction
GB201612011D0 (en) * 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
CN106701932B (en) * 2016-12-08 2019-09-27 江苏苏博生物医学股份有限公司 A kind of 21 short tandem repeat composite amplification detection kits using double fluorescence labeling method
GB201721307D0 (en) * 2017-12-19 2018-01-31 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides

Citations (3)

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Publication number Priority date Publication date Assignee Title
US6051377A (en) * 1995-11-30 2000-04-18 Pharmaseq, Inc. Multiplex assay for nucleic acids employing transponders
US6462190B1 (en) * 1999-04-14 2002-10-08 California Institute Of Technology Polynucleotides and kits for detection of an age-related mutation
US6709815B1 (en) * 1997-05-05 2004-03-23 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2187295A (en) * 1994-03-18 1995-10-09 General Hospital Corporation, The Cleaved amplified rflp detection methods
EP1320628A2 (en) * 2000-04-05 2003-06-25 Molecular Staging Inc. Process for allele discrimation utilizing primer extension
US7262030B2 (en) * 2001-05-09 2007-08-28 Virginia Commonwealth University Multiple sequencible and ligatible structures for genomic analysis
WO2005001129A2 (en) * 2003-06-06 2005-01-06 Applera Corporation Mobility cassettes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6051377A (en) * 1995-11-30 2000-04-18 Pharmaseq, Inc. Multiplex assay for nucleic acids employing transponders
US6709815B1 (en) * 1997-05-05 2004-03-23 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
US6462190B1 (en) * 1999-04-14 2002-10-08 California Institute Of Technology Polynucleotides and kits for detection of an age-related mutation

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BARANY F: "THE LIGASE CHAIN REACTION IN A PCR WORLD", PCR METHODS AND APPLICATIONS, COLD SPRING HARBOR, NY, US, vol. 1, no. 1, August 1991 (1991-08-01), pages 5 - 16, XP009024434, ISSN: 1054-9803 *
TOBE V O ET AL: "SINGLE-WELL GENOTYPING OF DIALLELIC SEQUENCE VARIATIONS BY A TWO-COLOR ELISA-BASED OLIGONUCLEOTIDE LIGATION ASSAY", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 24, no. 19, 1996, pages 3728 - 3732, XP000978683, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
US20060166235A1 (en) 2006-07-27
WO2006071568A2 (en) 2006-07-06

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