WO2006066001A3 - Immobilized degradable cationic polymer for transfecting eukaryotic cells - Google Patents
Immobilized degradable cationic polymer for transfecting eukaryotic cells Download PDFInfo
- Publication number
- WO2006066001A3 WO2006066001A3 PCT/US2005/045429 US2005045429W WO2006066001A3 WO 2006066001 A3 WO2006066001 A3 WO 2006066001A3 US 2005045429 W US2005045429 W US 2005045429W WO 2006066001 A3 WO2006066001 A3 WO 2006066001A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- transfection
- immobilized
- culture device
- eukaryotic cells
- cationic polymer
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/30—Synthetic polymers
Abstract
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP05854197A EP1836293A2 (en) | 2004-12-17 | 2005-12-14 | Immobilized degradable cationic polymer for transfecting eukaryotic cells |
CA002590820A CA2590820A1 (en) | 2004-12-17 | 2005-12-14 | Immobilized degradable cationic polymer for transfecting eukaryotic cells |
AU2005316501A AU2005316501B2 (en) | 2004-12-17 | 2005-12-14 | Immobilized degradable cationic polymer for transfecting eukaryotic cells |
JP2007546895A JP4739352B2 (en) | 2004-12-17 | 2005-12-14 | Solid surface with immobilized degradable cationic polymer for transfecting eukaryotic cells |
KR1020137000063A KR20130010036A (en) | 2004-12-17 | 2005-12-14 | Immobilized degradable cationic polymer for transfecting eukaryotic cells |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US63734404P | 2004-12-17 | 2004-12-17 | |
US60/637,344 | 2004-12-17 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2006066001A2 WO2006066001A2 (en) | 2006-06-22 |
WO2006066001A3 true WO2006066001A3 (en) | 2006-09-08 |
Family
ID=36353906
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2005/045429 WO2006066001A2 (en) | 2004-12-17 | 2005-12-14 | Immobilized degradable cationic polymer for transfecting eukaryotic cells |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP1836293A2 (en) |
JP (1) | JP4739352B2 (en) |
KR (2) | KR101263173B1 (en) |
CN (1) | CN101124316A (en) |
AU (1) | AU2005316501B2 (en) |
CA (1) | CA2590820A1 (en) |
WO (1) | WO2006066001A2 (en) |
Cited By (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8895311B1 (en) | 2001-03-28 | 2014-11-25 | Handylab, Inc. | Methods and systems for control of general purpose microfluidic devices |
US9028773B2 (en) | 2001-09-12 | 2015-05-12 | Handylab, Inc. | Microfluidic devices having a reduced number of input and output connections |
US9051604B2 (en) | 2001-02-14 | 2015-06-09 | Handylab, Inc. | Heat-reduction methods and systems related to microfluidic devices |
US9109229B2 (en) | 2004-07-26 | 2015-08-18 | Pfenex Inc. | Process for improved protein expression by strain engineering |
USD742027S1 (en) | 2011-09-30 | 2015-10-27 | Becton, Dickinson And Company | Single piece reagent holder |
US9186677B2 (en) | 2007-07-13 | 2015-11-17 | Handylab, Inc. | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
US9217143B2 (en) | 2007-07-13 | 2015-12-22 | Handylab, Inc. | Polynucleotide capture materials, and methods of using same |
US9222954B2 (en) | 2011-09-30 | 2015-12-29 | Becton, Dickinson And Company | Unitized reagent strip |
US9238223B2 (en) | 2007-07-13 | 2016-01-19 | Handylab, Inc. | Microfluidic cartridge |
US9259734B2 (en) | 2007-07-13 | 2016-02-16 | Handylab, Inc. | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
US9259735B2 (en) | 2001-03-28 | 2016-02-16 | Handylab, Inc. | Methods and systems for control of microfluidic devices |
US9347586B2 (en) | 2007-07-13 | 2016-05-24 | Handylab, Inc. | Automated pipetting apparatus having a combined liquid pump and pipette head system |
US9394571B2 (en) | 2007-04-27 | 2016-07-19 | Pfenex Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
US9453251B2 (en) | 2002-10-08 | 2016-09-27 | Pfenex Inc. | Expression of mammalian proteins in Pseudomonas fluorescens |
US9572886B2 (en) | 2005-12-22 | 2017-02-21 | Nitto Denko Corporation | Agent for treating myelofibrosis |
US9580719B2 (en) | 2007-04-27 | 2017-02-28 | Pfenex, Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
USD787087S1 (en) | 2008-07-14 | 2017-05-16 | Handylab, Inc. | Housing |
US11959126B2 (en) | 2006-03-24 | 2024-04-16 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7829025B2 (en) | 2001-03-28 | 2010-11-09 | Venture Lending & Leasing Iv, Inc. | Systems and methods for thermal actuation of microfluidic devices |
EP2402089A1 (en) | 2003-07-31 | 2012-01-04 | Handylab, Inc. | Processing particle-containing samples |
US8852862B2 (en) | 2004-05-03 | 2014-10-07 | Handylab, Inc. | Method for processing polynucleotide-containing samples |
US7358223B2 (en) | 2004-10-04 | 2008-04-15 | Nitto Denko Corporation | Biodegradable cationic polymers |
EP2001990B1 (en) | 2006-03-24 | 2016-06-29 | Handylab, Inc. | Integrated system for processing microfluidic samples, and method of using same |
US11806718B2 (en) | 2006-03-24 | 2023-11-07 | Handylab, Inc. | Fluorescence detector for microfluidic diagnostic system |
US7998708B2 (en) | 2006-03-24 | 2011-08-16 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
EP2091647A2 (en) | 2006-11-14 | 2009-08-26 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
WO2008060604A2 (en) | 2006-11-14 | 2008-05-22 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
US9618139B2 (en) | 2007-07-13 | 2017-04-11 | Handylab, Inc. | Integrated heater and magnetic separator |
US8182763B2 (en) | 2007-07-13 | 2012-05-22 | Handylab, Inc. | Rack for sample tubes and reagent holders |
ES2769028T3 (en) | 2011-04-15 | 2020-06-24 | Becton Dickinson Co | Real-time scanning microfluidic thermocycler |
EP2773892B1 (en) | 2011-11-04 | 2020-10-07 | Handylab, Inc. | Polynucleotide sample preparation device |
RU2658773C2 (en) | 2012-02-03 | 2018-06-22 | Бектон, Дикинсон Энд Компани | System and method of implementation of automated assays on plurality of biological samples |
CN102827756B (en) * | 2012-06-25 | 2014-04-09 | 华南农业大学 | Agrobacterium local invasion method applied in soybean hypocotyl explant transformation system, and device thereof |
JP6609246B2 (en) * | 2013-06-28 | 2019-11-20 | エスリス ゲーエムベーハー | Composition for introducing RNA into cells |
RU2718590C2 (en) * | 2014-11-10 | 2020-04-08 | Этрис Гмбх | Induction of osteogenesis by introduction of rna coding bone morphogenetic protein (bmp) |
BR112019006671A2 (en) * | 2016-10-05 | 2019-06-25 | Syngenta Participations Ag | improvements in or related to gene silencing |
CN109735573A (en) * | 2019-02-19 | 2019-05-10 | 武汉普诺赛生命科技有限公司 | A kind of transient transfection reagent and its application method |
EP3798250A1 (en) * | 2019-09-25 | 2021-03-31 | University College Dublin | Hyperbranched cationic polymers useful as nucleic acid delivery vectors for transfecting |
CN112195193A (en) * | 2020-09-30 | 2021-01-08 | 上海交通大学 | Application of single-stranded DNA in gene transfection |
CN114904003B (en) * | 2021-02-09 | 2023-09-29 | 广州立得生物医药科技有限公司 | Use of ionizable cationic lipid analog materials as nucleic acid drug delivery vehicles or transfection reagents |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030215395A1 (en) * | 2002-05-14 | 2003-11-20 | Lei Yu | Controllably degradable polymeric biomolecule or drug carrier and method of synthesizing said carrier |
EP1398042A1 (en) * | 2002-09-10 | 2004-03-17 | Lipotek, Inc | Transfection of nucleic acid |
US20040138154A1 (en) * | 2003-01-13 | 2004-07-15 | Lei Yu | Solid surface for biomolecule delivery and high-throughput assay |
WO2005023312A2 (en) * | 2003-09-03 | 2005-03-17 | Imperial College Innovations Limited | Methods for transfecting cells using dna minor groove binding agents |
US20050176132A1 (en) * | 2004-02-10 | 2005-08-11 | Yasunobu Tanaka | Culture device and method for eukaryotic cell transfection |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020006664A1 (en) * | 1999-09-17 | 2002-01-17 | Sabatini David M. | Arrayed transfection method and uses related thereto |
DE60031997D1 (en) * | 1999-09-17 | 2007-01-04 | Whitehead Biomedical Inst | UMKEHRTRANSFEKTIONSVERFAHREN |
WO2002042447A2 (en) * | 2000-11-03 | 2002-05-30 | Regents Of The University Of Michigan | Surface transfection and expression procedure |
JP2005529959A (en) * | 2002-06-14 | 2005-10-06 | マイラス コーポレイション | Novel method for delivering polynucleotides to cells |
US6878374B2 (en) * | 2003-02-25 | 2005-04-12 | Nitto Denko Corporation | Biodegradable polyacetals |
ATE477325T1 (en) * | 2004-02-18 | 2010-08-15 | Nitto Denko Corp | CULTURE DEVICE AND METHOD FOR TRANSFECTION OF EUKARYONTIC CELLS |
-
2005
- 2005-12-14 CN CNA2005800434531A patent/CN101124316A/en active Pending
- 2005-12-14 EP EP05854197A patent/EP1836293A2/en not_active Withdrawn
- 2005-12-14 KR KR1020077015797A patent/KR101263173B1/en not_active IP Right Cessation
- 2005-12-14 JP JP2007546895A patent/JP4739352B2/en not_active Expired - Fee Related
- 2005-12-14 WO PCT/US2005/045429 patent/WO2006066001A2/en active Application Filing
- 2005-12-14 AU AU2005316501A patent/AU2005316501B2/en not_active Ceased
- 2005-12-14 CA CA002590820A patent/CA2590820A1/en not_active Abandoned
- 2005-12-14 KR KR1020137000063A patent/KR20130010036A/en not_active Application Discontinuation
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030215395A1 (en) * | 2002-05-14 | 2003-11-20 | Lei Yu | Controllably degradable polymeric biomolecule or drug carrier and method of synthesizing said carrier |
EP1398042A1 (en) * | 2002-09-10 | 2004-03-17 | Lipotek, Inc | Transfection of nucleic acid |
US20040138154A1 (en) * | 2003-01-13 | 2004-07-15 | Lei Yu | Solid surface for biomolecule delivery and high-throughput assay |
WO2005023312A2 (en) * | 2003-09-03 | 2005-03-17 | Imperial College Innovations Limited | Methods for transfecting cells using dna minor groove binding agents |
US20050176132A1 (en) * | 2004-02-10 | 2005-08-11 | Yasunobu Tanaka | Culture device and method for eukaryotic cell transfection |
Cited By (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9051604B2 (en) | 2001-02-14 | 2015-06-09 | Handylab, Inc. | Heat-reduction methods and systems related to microfluidic devices |
US9528142B2 (en) | 2001-02-14 | 2016-12-27 | Handylab, Inc. | Heat-reduction methods and systems related to microfluidic devices |
US9259735B2 (en) | 2001-03-28 | 2016-02-16 | Handylab, Inc. | Methods and systems for control of microfluidic devices |
US8895311B1 (en) | 2001-03-28 | 2014-11-25 | Handylab, Inc. | Methods and systems for control of general purpose microfluidic devices |
US9028773B2 (en) | 2001-09-12 | 2015-05-12 | Handylab, Inc. | Microfluidic devices having a reduced number of input and output connections |
US9458487B2 (en) | 2002-10-08 | 2016-10-04 | Pfenex, Inc. | Expression of mammalian proteins in pseudomonas fluorescens |
US9453251B2 (en) | 2002-10-08 | 2016-09-27 | Pfenex Inc. | Expression of mammalian proteins in Pseudomonas fluorescens |
US9109229B2 (en) | 2004-07-26 | 2015-08-18 | Pfenex Inc. | Process for improved protein expression by strain engineering |
US9572886B2 (en) | 2005-12-22 | 2017-02-21 | Nitto Denko Corporation | Agent for treating myelofibrosis |
US11959126B2 (en) | 2006-03-24 | 2024-04-16 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
US9394571B2 (en) | 2007-04-27 | 2016-07-19 | Pfenex Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
US9580719B2 (en) | 2007-04-27 | 2017-02-28 | Pfenex, Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
US9347586B2 (en) | 2007-07-13 | 2016-05-24 | Handylab, Inc. | Automated pipetting apparatus having a combined liquid pump and pipette head system |
US9259734B2 (en) | 2007-07-13 | 2016-02-16 | Handylab, Inc. | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
US9238223B2 (en) | 2007-07-13 | 2016-01-19 | Handylab, Inc. | Microfluidic cartridge |
US9217143B2 (en) | 2007-07-13 | 2015-12-22 | Handylab, Inc. | Polynucleotide capture materials, and methods of using same |
US9186677B2 (en) | 2007-07-13 | 2015-11-17 | Handylab, Inc. | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
US11845081B2 (en) | 2007-07-13 | 2023-12-19 | Handylab, Inc. | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
USD787087S1 (en) | 2008-07-14 | 2017-05-16 | Handylab, Inc. | Housing |
US9222954B2 (en) | 2011-09-30 | 2015-12-29 | Becton, Dickinson And Company | Unitized reagent strip |
USD742027S1 (en) | 2011-09-30 | 2015-10-27 | Becton, Dickinson And Company | Single piece reagent holder |
Also Published As
Publication number | Publication date |
---|---|
JP4739352B2 (en) | 2011-08-03 |
KR101263173B1 (en) | 2013-05-13 |
JP2008523810A (en) | 2008-07-10 |
KR20130010036A (en) | 2013-01-24 |
WO2006066001A2 (en) | 2006-06-22 |
CA2590820A1 (en) | 2006-06-22 |
AU2005316501B2 (en) | 2010-10-21 |
AU2005316501A1 (en) | 2006-06-22 |
CN101124316A (en) | 2008-02-13 |
EP1836293A2 (en) | 2007-09-26 |
KR20070101859A (en) | 2007-10-17 |
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