WO2006050275A3 - Rapid species-specific detection of pathogens - Google Patents

Rapid species-specific detection of pathogens Download PDF

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Publication number
WO2006050275A3
WO2006050275A3 PCT/US2005/039292 US2005039292W WO2006050275A3 WO 2006050275 A3 WO2006050275 A3 WO 2006050275A3 US 2005039292 W US2005039292 W US 2005039292W WO 2006050275 A3 WO2006050275 A3 WO 2006050275A3
Authority
WO
WIPO (PCT)
Prior art keywords
pathogen
specimen
lysate
pathogens
nucleic acid
Prior art date
Application number
PCT/US2005/039292
Other languages
French (fr)
Other versions
WO2006050275A2 (en
Inventor
Joseph C Liao
David A Haake
Bernard M Churchill
Marc A Suchard
Original Assignee
Univ California
Joseph C Liao
David A Haake
Bernard M Churchill
Marc A Suchard
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California, Joseph C Liao, David A Haake, Bernard M Churchill, Marc A Suchard filed Critical Univ California
Publication of WO2006050275A2 publication Critical patent/WO2006050275A2/en
Publication of WO2006050275A3 publication Critical patent/WO2006050275A3/en
Priority to US11/743,071 priority Critical patent/US7763426B2/en
Priority to US12/788,101 priority patent/US20110027782A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Abstract

The invention provides a method for detecting the presence of a pathogen in a specimen. The method comprises contacting a lysate of the specimen with a capture probe immobilized on a substrate. The lysate can be prepared rapidly from a clinical specimen, obviating the need for specimen culture or bacterial purification prior to assay. The capture probe comprises a first oligonucleotide that specifically hybridizes with a first nucleic acid sequence of the pathogen to be detected. The lysate has been brought into contact with a detector probe that comprises a detectably labeled oligonucleotide that specifically hybridizes with a second nucleic acid sequence of the pathogen. Detection of the second detectable label complexed with the substrate is indicative of the presence of the corresponding pathogen. Variations of the method that optimize detection are described. Also provided are probes and kits for use in the method.
PCT/US2005/039292 2004-11-01 2005-11-01 Rapid species-specific detection of pathogens WO2006050275A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US11/743,071 US7763426B2 (en) 2004-11-01 2007-05-01 Probes and methods for detection of Escheridia coli and antibiotic resistance
US12/788,101 US20110027782A1 (en) 2004-11-01 2010-05-26 Probes and methods for detection of pathogens and antibiotic resistance

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US62390304P 2004-11-01 2004-11-01
US60/623,903 2004-11-01

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/743,071 Continuation-In-Part US7763426B2 (en) 2004-11-01 2007-05-01 Probes and methods for detection of Escheridia coli and antibiotic resistance

Publications (2)

Publication Number Publication Date
WO2006050275A2 WO2006050275A2 (en) 2006-05-11
WO2006050275A3 true WO2006050275A3 (en) 2007-04-12

Family

ID=36319743

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/039292 WO2006050275A2 (en) 2004-11-01 2005-11-01 Rapid species-specific detection of pathogens

Country Status (1)

Country Link
WO (1) WO2006050275A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103773842A (en) * 2013-10-18 2014-05-07 南京农业大学 Specific nanogold DNA probe for detecting urinary tract infection pathogenic bacteria
WO2017088169A1 (en) * 2015-11-27 2017-06-01 Coyote Bioscience Co., Ltd. Methods and systems for nucleic acid amplification

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6348314B1 (en) * 1996-01-24 2002-02-19 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US6391558B1 (en) * 1997-03-18 2002-05-21 Andcare, Inc. Electrochemical detection of nucleic acid sequences

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6348314B1 (en) * 1996-01-24 2002-02-19 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US6391558B1 (en) * 1997-03-18 2002-05-21 Andcare, Inc. Electrochemical detection of nucleic acid sequences

Also Published As

Publication number Publication date
WO2006050275A2 (en) 2006-05-11

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