WO2006044078A3 - Apparatus and method for analysis of molecules - Google Patents

Apparatus and method for analysis of molecules Download PDF

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Publication number
WO2006044078A3
WO2006044078A3 PCT/US2005/033052 US2005033052W WO2006044078A3 WO 2006044078 A3 WO2006044078 A3 WO 2006044078A3 US 2005033052 W US2005033052 W US 2005033052W WO 2006044078 A3 WO2006044078 A3 WO 2006044078A3
Authority
WO
WIPO (PCT)
Prior art keywords
molecules
analysis
present
methods
throughput
Prior art date
Application number
PCT/US2005/033052
Other languages
French (fr)
Other versions
WO2006044078A2 (en
Inventor
Stephen Turner
Jonas Korlach
Original Assignee
Pacific Biosciences California
Stephen Turner
Jonas Korlach
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/944,106 external-priority patent/US7170050B2/en
Application filed by Pacific Biosciences California, Stephen Turner, Jonas Korlach filed Critical Pacific Biosciences California
Priority to GB0608338A priority Critical patent/GB2423819B/en
Priority to AU2005296200A priority patent/AU2005296200B2/en
Priority to CN2005800314830A priority patent/CN101199241B/en
Priority to CA2579150A priority patent/CA2579150C/en
Priority to EP05812440A priority patent/EP1790202A4/en
Priority to JP2007532480A priority patent/JP2008513782A/en
Priority to EP18184493.7A priority patent/EP3415641B1/en
Publication of WO2006044078A2 publication Critical patent/WO2006044078A2/en
Publication of WO2006044078A3 publication Critical patent/WO2006044078A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/10—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type
    • G02B6/12—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type of the integrated circuit kind
    • G02B6/13—Integrated optical circuits characterised by the manufacturing method
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0896—Nanoscaled
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing

Abstract

The present invention relates to optical confinements, method of preparing and methods of using them for analyzing molecules and/or monitoring chemical reactions. The apparatus and methods embodied in the present invention are particularly useful for high-throughput and low-cost single-molecular analysis.
PCT/US2005/033052 2004-09-17 2005-09-16 Apparatus and method for analysis of molecules WO2006044078A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
GB0608338A GB2423819B (en) 2004-09-17 2005-09-16 Apparatus and method for analysis of molecules
AU2005296200A AU2005296200B2 (en) 2004-09-17 2005-09-16 Apparatus and method for analysis of molecules
CN2005800314830A CN101199241B (en) 2004-09-17 2005-09-16 Device and method for molecule analysis
CA2579150A CA2579150C (en) 2004-09-17 2005-09-16 Apparatus and method for analysis of molecules
EP05812440A EP1790202A4 (en) 2004-09-17 2005-09-16 Apparatus and method for analysis of molecules
JP2007532480A JP2008513782A (en) 2004-09-17 2005-09-16 Apparatus and method for molecular analysis
EP18184493.7A EP3415641B1 (en) 2004-09-17 2005-09-16 Method for analysis of molecules

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US10/944,106 US7170050B2 (en) 2004-09-17 2004-09-17 Apparatus and methods for optical analysis of molecules
US10/944,106 2004-09-17
US64900905P 2005-01-31 2005-01-31
US60/649,009 2005-01-31
US65184605P 2005-02-09 2005-02-09
US60/651,846 2005-02-09

Publications (2)

Publication Number Publication Date
WO2006044078A2 WO2006044078A2 (en) 2006-04-27
WO2006044078A3 true WO2006044078A3 (en) 2008-02-21

Family

ID=36073574

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/033052 WO2006044078A2 (en) 2004-09-17 2005-09-16 Apparatus and method for analysis of molecules

Country Status (8)

Country Link
US (4) US7476503B2 (en)
EP (2) EP1790202A4 (en)
JP (3) JP2008513782A (en)
CN (1) CN101914620B (en)
AU (1) AU2005296200B2 (en)
CA (1) CA2579150C (en)
GB (1) GB2423819B (en)
WO (1) WO2006044078A2 (en)

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