WO2006031815A3 - Methods and compositions for proximity assays - Google Patents

Methods and compositions for proximity assays Download PDF

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Publication number
WO2006031815A3
WO2006031815A3 PCT/US2005/032570 US2005032570W WO2006031815A3 WO 2006031815 A3 WO2006031815 A3 WO 2006031815A3 US 2005032570 W US2005032570 W US 2005032570W WO 2006031815 A3 WO2006031815 A3 WO 2006031815A3
Authority
WO
WIPO (PCT)
Prior art keywords
unit
signal
transmitter
compositions
producing
Prior art date
Application number
PCT/US2005/032570
Other languages
French (fr)
Other versions
WO2006031815A2 (en
Inventor
Sharat Singh
Original Assignee
Monogram Biosciences Inc
Sharat Singh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monogram Biosciences Inc, Sharat Singh filed Critical Monogram Biosciences Inc
Publication of WO2006031815A2 publication Critical patent/WO2006031815A2/en
Publication of WO2006031815A3 publication Critical patent/WO2006031815A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Abstract

The present invention is directed to methods and compositions for performing proximity assays in samples of fixed specimens. In particular, the assays are useful for the detection of multicomponent complexes of biological molecules such as proteins, peptides, hormones, DNA, RNA and the like. In one embodiment, the method comprises providing a signal generating system comprised of a first unit capable of enzymatically producing a transmitter and having a first binding moiety, and a second unit dependent on the transmitter for producing a signal molecule and having a second binding moiety, combining under binding conditions the sample and the signal generating system such that the first and second binding moieties bind to their respective binding sites on different components of the complex such that the second unit is within the effective proximity of the transmitter produced by the first unit so that signal molecules are produced, and analyzing for the presence of signal molecules, whereby the multicomponent complex is detected. Also provided are compositions that have an enzymatic unit for producing a transmitter that then non-enzymatically generates a signal at the second unit.
PCT/US2005/032570 2004-09-13 2005-09-13 Methods and compositions for proximity assays WO2006031815A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US60930104P 2004-09-13 2004-09-13
US60/609,301 2004-09-13

Publications (2)

Publication Number Publication Date
WO2006031815A2 WO2006031815A2 (en) 2006-03-23
WO2006031815A3 true WO2006031815A3 (en) 2006-08-17

Family

ID=36060644

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/032570 WO2006031815A2 (en) 2004-09-13 2005-09-13 Methods and compositions for proximity assays

Country Status (1)

Country Link
WO (1) WO2006031815A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9575066B2 (en) 2006-09-21 2017-02-21 Nestec S.A. Antibody-based arrays for detecting multiple signal transducers in rare circulating cells

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011008990A1 (en) 2009-07-15 2011-01-20 Prometheus Laboratories Inc. Drug selection for gastric cancer therapy using antibody-based arrays
RU2519647C2 (en) * 2007-07-13 2014-06-20 Нестек С.А. Selection of medications for lung cancer therapy by means of antibody-based matrices
CA2923248A1 (en) 2008-02-25 2009-09-03 Nestec S.A. Methods for detecting truncated receptors
EP2388324A1 (en) * 2010-05-20 2011-11-23 Siemens Healthcare Diagnostics Inc. Control material for nucleic acid tests
US9719995B2 (en) 2011-02-03 2017-08-01 Pierian Holdings, Inc. Drug selection for colorectal cancer therapy using receptor tyrosine kinase profiling
WO2013033623A1 (en) 2011-09-02 2013-03-07 Nestec S.A. Profiling of signal pathway proteins to determine therapeutic efficacy
EP3336546B1 (en) * 2016-12-13 2020-07-01 Miltenyi Biotec B.V. & Co. KG Reversible cell labelling with conjugates having two releasable binding sites

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4281061A (en) * 1979-07-27 1981-07-28 Syva Company Double antibody for enhanced sensitivity in immunoassay
US4758080A (en) * 1986-12-18 1988-07-19 Cornell Research Foundation, Inc. Pointspread retinoscope
US5532138A (en) * 1990-04-26 1996-07-02 Behringwerke Ag Method and kits for determining peroxidatively active catalysts
US5843666A (en) * 1994-09-02 1998-12-01 Lumigen, Inc. Chemiluminescent detection methods using dual enzyer-labeled binding partners
US6159699A (en) * 1994-05-10 2000-12-12 Molecular Light Technology Limited Enzyme linked chemiluminescent assay

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4281061A (en) * 1979-07-27 1981-07-28 Syva Company Double antibody for enhanced sensitivity in immunoassay
US4758080A (en) * 1986-12-18 1988-07-19 Cornell Research Foundation, Inc. Pointspread retinoscope
US5532138A (en) * 1990-04-26 1996-07-02 Behringwerke Ag Method and kits for determining peroxidatively active catalysts
US6159699A (en) * 1994-05-10 2000-12-12 Molecular Light Technology Limited Enzyme linked chemiluminescent assay
US5843666A (en) * 1994-09-02 1998-12-01 Lumigen, Inc. Chemiluminescent detection methods using dual enzyer-labeled binding partners

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9575066B2 (en) 2006-09-21 2017-02-21 Nestec S.A. Antibody-based arrays for detecting multiple signal transducers in rare circulating cells

Also Published As

Publication number Publication date
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