WO2006023927A1 - Method for determining the concentration of analytes in samples by direct mediation of enzymes - Google Patents
Method for determining the concentration of analytes in samples by direct mediation of enzymes Download PDFInfo
- Publication number
- WO2006023927A1 WO2006023927A1 PCT/US2005/029985 US2005029985W WO2006023927A1 WO 2006023927 A1 WO2006023927 A1 WO 2006023927A1 US 2005029985 W US2005029985 W US 2005029985W WO 2006023927 A1 WO2006023927 A1 WO 2006023927A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- glucose
- factor
- pqq
- gdh
- tetrazolium salt
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
- Y10T436/144444—Glucose
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/17—Nitrogen containing
- Y10T436/173076—Nitrite or nitrate
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
Definitions
- This invention relates generally to methods for determining the amount of an analyte in biological fluids. More particularly, the invention includes methods for measuring the glucose content of blood or other fluids.
- the quantitative determination of analytes in biological fluids such as whole blood is of great importance in the diagnosing and treating of certain medical conditions. For example, determining the glucose level in the blood of diabetic individuals, who must frequently check the glucose level in their blood to regulate their diets and medication. Measuring the glucose content of blood can be done by several methods. One method employs an electrochemical biosensor. Another method provides a visual indication of the glucose content such as by developing a color by reaction of an indicator. The present invention is of the later type, although it may have application in electrochemical methods as well.
- Electrochemical methods have been described in many patents. They may be divided into several categories including coulometic, amperometric, and cyclic voltammetry.
- One recent published U.S. patent application is 2003/0094384Al.
- Japanese published application 2000171428A2 describes the preparation of a glucose electrolytic sensor.
- glucose oxidase enzymes have been used widely in dry strips and in other techniques for measuring the glucose contact of blood.
- Various indicators have been employed in glucose sensors, such as benzidine-type indicators and heterocyclic azines. For example, 3,3',5,5'- tetramethylbenzidine and syringaldazine, luminol, o-tolidine, o-dianisitine, among others.
- One family of indicators is that of tetrazolium dye precursors. Examples of patents describing such indicators include U.S. 5,126,275, U.S. 5,322,680, U.S. 5,300,637, U.S. 5,290,536, U.S. 5,360,595 and U.S. 6,586,199. Tetrazolium indicators are used in the invention to be described below.
- PQQ pyrroloquinoline quinone or its derivatives or isomers
- GDH glucose dehydrogenase
- glucose dehydrogenase - co-factor combinations can be used to provide a colorimetric response from tetrazolium indicators when they are in contact with glucose solutions but, without including the mediator that is normally required.
- PQQ-GDH PQQ dependent glucose dehydrogenase
- Other quinone-dependent dehydrogenase enzymes are expected to provide similar performance with their corresponding donor compounds.
- the invention is a reagent solution for measuring the concentration of glucose in blood.
- a glucose dehydrogenase (GDH) dependent on pyrroloquinoline quinone (PQQ) (or its derivatives or isomers) as a co-factor is used with a tetrazolium dye precursor, but without including a mediator such as PMS (phenazine methosulfate) or a diaphorase.
- the invention is a dry test strip containing a GDH, PQQ (or a derivative or isomer) as a co-factor, and a tetrazolium dye precursor, but lacking the usual mediator.
- the strip will be contacted with glucose-containing blood and the developed color measured and correlated with the glucose content.
- the invention also is an improved method of measuring the glucose content of whole blood using the test strips containing GDH and PQQ (or a derivative or isomer) as a co-factor, and a tetrazolium dye precursor in the substantial absence of a mediator.
- Fig. 1 is a graph of the results of Example 1.
- Fig. 2 is a graph of the results of Example 2.
- Fig. 3 is a graph of the results of Example 3.
- Fig. 4 is a graph of the results of Example 4.
- glucose oxidase In the methods which employ glucose oxidase, those enzymes are reacted with glucose, producing oxidized glucose and hydrogen peroxide.
- the hydrogen peroxide oxidizes an indicator compound in the presence of a peroxidase.
- the oxidized indicator produces a color that is correlated with the glucose content of the blood sample.
- a glucose dehydrogenase is used, along with a co-factor and a tetrazolium dye precursor to produce a colored response proportional to the glucose content of the sample.
- Such reactions are normally described by the following sequence of reactions:
- the mediator may be any one of those familiar to those skilled in the art, such as PMS (phenazine methosulfate), PES (phenazine ethosulfate), DCIP (2,6,-dichlorophenolindophenol), and ferrocene.
- the mediator has been considered to be necessary to obtain a useful response by reducing the tetrazolium indicator to formazan. However, in the present invention, a mediator is not needed and a useful response is obtained in its absence, as will be shown in the examples below.
- the mediator is not required when the glucose dehydrogenase is classified as EC 1.1.99.17 (now ECl.1.5.2) (International Union of Biochemistry and Molecular Biology; Nomenclature and Classification of Enzyme-Catalyzed Reactions; NC-IUBMB).
- Such enzymes are dependent on certain co- factors, in this instance the co-factor is pyrroloquinoline quinone (PQQ) or derivatives or isomers thereof.
- PQQ pyrroloquinoline quinone
- Other glucose dehydrogenase enzymes are dependent on other co ⁇ enzymes or co-factors, such as nicotinamide adenine dinucleotide (NAD), flavin adenine dinueleotide (FAD), and their analogs.
- NAD nicotinamide adenine dinucleotide
- FAD flavin adenine dinueleotide
- Glucose dehydrogenase enzymes are commercially available from Toyoba, Kyowa, Amano, Genzyme, Biozyme, among others and are either native enzymes or recombinant enzymes produced by classic fermentation and/or recombinant methods.
- dehydrogenases require a co-factor, such as NAD, FAD and PQQ and their derivatives or isomers. Since the dehydrogenases have been obtained from their suppliers in combination with a co-factor they have been characterized by their suppliers and their nature is not fully known at this time. Combinations of dehydrogenase enzymes with known co-factors have been tested, with the results shown in the examples. As will be seen, PQQ-dependent GDH enzymes did not require the presence of a mediator.
- glucose dehydrogenase dependent on PQQ did not require a mediator in order to measure reaction with glucose in the presence of a tetrazolium salt indicator. They believe that other dehydrogenase enzymes with quinones as acceptor (EC 1.1.5 class) may behave similarly in oxidizing alcohols, or other CH-OH donor compounds.
- mediators it has been considered necessary to include in a test system a mediator to reoxidize the reduced dehydrogenase-co-factor after the reaction with glucose to form the corresponding lactone.
- a diaphorase or PMS phenazine methosulfate
- PMS phenazine methosulfate
- a mediator is not needed. As will be shown in the examples below, the new reagent system will be seen to behave in a similar manner whether or not mediators are included.
- Tetrazolium indicators are generally described in U.S. patents mentioned earlier. In U.S. 6,200,773 certain tetrazolium dye precursors are listed as being particularly useful in reactions with dehydrogenase-co-factor combinations. Among them is the tetrazolium compound designated WST-4, used in the examples below.
- Example 1
- a strip of 0.8 ⁇ m polyethersulfone membrane was dipped in a solution containing 370 mM phosphate buffer, pH 7, 0.5% polymer (e.g. Teleostatin), surfactants (e.g., 0.1% Cremaphor EL and 0.2% Triton X305), 5.5 mg of Non-NAD(P)-dependent Glucose dehydrogenase [a quinone-dependent GDH designated EC 1.1.99.17 (now EC 1.1.5.2)] from Toyobo, 800 U/mg, and 60 mM of indicator (WST-4 from Dojindo), but containing no mediator.
- Glucose samples were prepared from 40% hematocrit whole blood samples and the concentration measured using the YSI glucose analyzer (Yellow Springs Instruments) as follows: a) 0 mg/dL human plasma b) 48 mg/dL human plasma c) 101 mg/dL human plasma d) 201 mg/dL human plasma e) 397 mg/dL human plasma f) 607 mg/dL human plasma
- Example 2 The results of Example 1 show that a measurable and substantially linear response was obtained even though no mediator (e.g., PMS) as described in the classic art of dehydrogenase based glucose sensors, was included in the reaction mixture.
- a mediator e.g., PMS
- PET polyethyleneterepthalate
- inactive ingredients e.g., rheology modifying agents, opacifiers, and surfactants.
- a mixture included 0.6 g/L polyacrylate, 0.6g/L polyvinyl alcohol, 1.5 g/L titanium dioxide, lg/L calcium carbonate, 0.001 to 0.006% of Silwet L-7600, Gerepon T-77, Surfonyl DF37, and containing 120 mM indicator (e.g., WST-4) and 6 KU/ mL Non-NAD(P)-dependent Glucose Dehydrogenase (ECl .1.99.17, now ECl.1.5.2 from Bayer, patent pending).
- Another strip was coated with the same mixture to which 5 mM of mediator (PMS) was added.
- Glucose samples were prepared from 40% hematocrit whole blood samples and the concentration of each measured using the YSI glucose analyzer (Yellow Springs Instruments) as follows: a) 1 mg/dL human plasma b) 56 mg/dL human plasma c) 112 mg/dL human plasma d) 224 mg/dL human plasma e) 448 mg/dL human plasma f) 678 mg/dL human plasma
- the mean reflectance of two replicates for each of the glucose samples was recorded at both 7 and 33 seconds after the sample was applied to the glucose reagent.
- Example 2 shows that a measurable and substantially linear response was obtained with the direct mediation method and that the observed response was substantially the same as that of the classic mediation method.
- the speed of the detection was also demonstrated to be substantially the same as the classic detection method as is evidenced by the endpoint reflectance measured at test times of 7 seconds and 33 seconds.
- Example 3 shows that a measurable and substantially linear response was obtained with the direct mediation method and that the observed response was substantially the same as that of the classic mediation method.
- the speed of the detection was also demonstrated to be substantially the same as the classic detection method as is evidenced by the endpoint reflectance measured at test times of 7 seconds and 33 seconds.
- Example 2 was repeated except that a different tetrazolium salt indicator was used.
- a strip of 0.8 ⁇ m polyethersulfone membrane (Osmonics) was dipped in a solution containing NAD as a co-factor with NAD-dependent GDH (EC 1.1.1.47) and a tetrazolium salt indicator, but without a mediator.
- the treated strip was contacted with glucose solutions having known concentrations. It was found that substantially no response was measured, as can be seen in Fig. 4.
- a reagent for measuring the concentration of glucose in blood comprising:
- GDH glucose dehydrogenase
- a reagent of embodiment A wherein said glucose dehydrogenase of (a) is classified according to NC-IUBMB as EC 1.1.99.17 (now EC 1.1.5.2).
- a reagent of embodiment A wherein said co-factor is PQQ.
- a reagent of embodiment A wherein said co-factor is a PQQ derivative or isomer.
- a reagent of embodiment A wherein said tetrazolium salt indicator is an analog of HTC-45.
- a test strip for measuring the glucose in blood comprising:
- GDH glucose dehydrogenase
- GDH and co-factor being capable of reducing to a colored formazan a tetrazolium salt indicator in the substantial absence of a mediator
- test strip of embodiment G wherein said co-factor is PQQ.
- test strip of embodiment G wherein said co-factor is a PQQ derivative or isomer.
- a test strip of embodiment G wherein said tetrazolium salt indicator is WST-4.
- test strip of embodiment G wherein said tetrazolium salt indicator is an analog of HTC-45.
- a method for measuring glucose in blood comprising:
- a reagent system disposed on said support, said reagent system comprising a glucose dehydrogenase (GDH), as a co-factor for GDH pyrrolo-quinoline quinono (PQQ) or a (PQQ) analog, said GDH and co-factor being capable of reducing a colored formazan a tetrazolium salt indicator in the absence of a mediator, and a tetrazolium salt indicator; (c) measuring the color developed from said tetrazolium salt indicator; and
- GDH glucose dehydrogenase
- step (d) correlating the color developed in step (b) with the glucose content of said blood sample.
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- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
Description
Claims
Priority Applications (9)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007530054A JP2008510490A (en) | 2004-08-24 | 2005-08-22 | A method for measuring the concentration of an analyte in a sample by direct mediation of an enzyme |
EP05794356A EP1831392B1 (en) | 2004-08-24 | 2005-08-22 | Method for determining the concentration of glucose by direct mediation of enzymes |
MX2007002224A MX2007002224A (en) | 2004-08-24 | 2005-08-22 | Method for determining the concentration of analytes in samples by direct mediation of enzymes. |
AT05794356T ATE473291T1 (en) | 2004-08-24 | 2005-08-22 | METHOD FOR DETERMINING THE CONCENTRATION OF GLUCOSE BY DIRECT MEDIATION OF ENZYMES |
BRPI0514542-2A BRPI0514542A (en) | 2004-08-24 | 2005-08-22 | method for determining the concentration of substances analyzed in samples by direct enzyme mediation |
CA002577688A CA2577688A1 (en) | 2004-08-24 | 2005-08-22 | Method for determining the concentration of analytes in samples by direct mediation of enzymes |
US11/660,250 US7867728B2 (en) | 2004-08-24 | 2005-08-22 | Determining the concentration of analytes in sample by direct mediation of enzymes |
DE602005022228T DE602005022228D1 (en) | 2004-08-24 | 2005-08-22 | METHOD FOR DETERMINING THE CONCENTRATION OF GLUCOSE BY DIRECT ENGINATION OF ENZYMES |
NO20071564A NO20071564L (en) | 2004-08-24 | 2007-03-26 | Process for determining the concentration of analytes in samples by direct transfer of enzymes |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US60388104P | 2004-08-24 | 2004-08-24 | |
US60/603,881 | 2004-08-24 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2006023927A1 true WO2006023927A1 (en) | 2006-03-02 |
Family
ID=35482305
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2005/029985 WO2006023927A1 (en) | 2004-08-24 | 2005-08-22 | Method for determining the concentration of analytes in samples by direct mediation of enzymes |
Country Status (13)
Country | Link |
---|---|
US (1) | US7867728B2 (en) |
EP (1) | EP1831392B1 (en) |
JP (1) | JP2008510490A (en) |
CN (1) | CN101006185A (en) |
AT (1) | ATE473291T1 (en) |
BR (1) | BRPI0514542A (en) |
CA (1) | CA2577688A1 (en) |
DE (1) | DE602005022228D1 (en) |
MX (1) | MX2007002224A (en) |
NO (1) | NO20071564L (en) |
RU (1) | RU2007110832A (en) |
TW (1) | TW200621996A (en) |
WO (1) | WO2006023927A1 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9599552B2 (en) | 2012-04-19 | 2017-03-21 | Roche Diabetes Care, Inc. | Devices and methods of determining disturbance variable-corrected analyte concentrations |
EP3412763A4 (en) * | 2016-02-04 | 2019-10-23 | Terumo Kabushiki Kaisha | Blood-sugar-level measurement reagent, blood-sugar-level measurement chip, and blood-sugar-level measurement device set |
EP3578634A4 (en) * | 2017-07-14 | 2020-12-23 | Terumo Kabushiki Kaisha | Blood sugar level measurement chip and blood sugar level measurement device set |
JPWO2019171731A1 (en) * | 2018-03-07 | 2021-03-18 | テルモ株式会社 | Methods, reagents and chips for measuring the concentration of analytical objects in biological samples |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20130020807A (en) * | 2008-11-07 | 2013-02-28 | 에프. 호프만-라 로슈 아게 | Fine-grained filler substances for photometric reaction films |
BR112014028616B1 (en) | 2012-06-22 | 2020-12-29 | F. Hoffmann-La Roche Ag | method for detecting at least one analyte in at least one sample of a body fluid, device and test system |
CN110176031B (en) * | 2013-08-09 | 2021-07-06 | 杭州堃博生物科技有限公司 | Method for evaluating registration between finished two-dimensional fluoroscopic image and three-dimensional fluoroscopic projection image of breast |
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EP0990706A1 (en) * | 1998-09-28 | 2000-04-05 | Lifescan, Inc. | Diagnostics based on tetrazolium compounds |
EP1367120A2 (en) * | 2002-05-27 | 2003-12-03 | Toyo Boseki Kabushiki Kaisha | Modified pyrroloquinoline quinone (PQQ) dependent glucose dehydrogenase with superior substrate specificity and stability |
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DE3529094A1 (en) | 1985-03-13 | 1986-09-18 | Miles Laboratories, Inc., Elkhart, Ind. | PROTEINS IMMOBILIZED ON POLYAMIDE OR CELLULOSEHYDRATE AND THE USE THEREOF FOR THE PRODUCTION OF BIOCATALYSTS, TEST STRIPS OR CHROMATOGRAPHY MATERIALS |
US5116729A (en) | 1989-03-10 | 1992-05-26 | Miles Inc. | Stabilization of oxidase enzyme-based test strips |
US5620863A (en) | 1989-08-28 | 1997-04-15 | Lifescan, Inc. | Blood glucose strip having reduced side reactions |
CA2049237C (en) | 1990-09-19 | 1999-10-19 | Gunther Beck | 2-thiazolyl tetrazolium salt indicators |
US5290536A (en) | 1990-09-19 | 1994-03-01 | Miles Inc. | Phenyl substituted 2-thiazolyl tetrazolium salt indicators |
US5300637A (en) | 1990-09-19 | 1994-04-05 | Miles Inc. | 2-benzothiazolyl tetrazolium salt indicators |
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JPH10243786A (en) | 1997-03-03 | 1998-09-14 | Koji Hayade | Modified glucose dehydrogenase |
JPH11243949A (en) | 1998-03-03 | 1999-09-14 | Toyobo Co Ltd | Glucose dehydrogenase having pqq as prosthetic group and its production |
JP3694424B2 (en) | 1998-09-29 | 2005-09-14 | 松下電器産業株式会社 | Glucose sensor |
JP2000350588A (en) | 1999-04-08 | 2000-12-19 | Koji Hayade | Glucose dehydrogenase |
JP2001204473A (en) | 2000-01-21 | 2001-07-31 | Toyobo Co Ltd | New plasmid, transformant containing the same, and method for producing enzyme using the same |
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US20030077702A1 (en) | 2001-10-23 | 2003-04-24 | Rajiv Shah | Method for formulating a glucose oxidase enzyme with a desired property or properties and a glucose oxidase enzyme with the desired property |
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-
2005
- 2005-08-22 EP EP05794356A patent/EP1831392B1/en not_active Not-in-force
- 2005-08-22 JP JP2007530054A patent/JP2008510490A/en not_active Withdrawn
- 2005-08-22 BR BRPI0514542-2A patent/BRPI0514542A/en not_active IP Right Cessation
- 2005-08-22 DE DE602005022228T patent/DE602005022228D1/en active Active
- 2005-08-22 CN CN200580028280.6A patent/CN101006185A/en active Pending
- 2005-08-22 AT AT05794356T patent/ATE473291T1/en not_active IP Right Cessation
- 2005-08-22 US US11/660,250 patent/US7867728B2/en not_active Expired - Fee Related
- 2005-08-22 MX MX2007002224A patent/MX2007002224A/en unknown
- 2005-08-22 WO PCT/US2005/029985 patent/WO2006023927A1/en active Application Filing
- 2005-08-22 RU RU2007110832/13A patent/RU2007110832A/en not_active Application Discontinuation
- 2005-08-22 CA CA002577688A patent/CA2577688A1/en not_active Abandoned
- 2005-08-24 TW TW094128856A patent/TW200621996A/en unknown
-
2007
- 2007-03-26 NO NO20071564A patent/NO20071564L/en not_active Application Discontinuation
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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EP0990706A1 (en) * | 1998-09-28 | 2000-04-05 | Lifescan, Inc. | Diagnostics based on tetrazolium compounds |
EP1367120A2 (en) * | 2002-05-27 | 2003-12-03 | Toyo Boseki Kabushiki Kaisha | Modified pyrroloquinoline quinone (PQQ) dependent glucose dehydrogenase with superior substrate specificity and stability |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9599552B2 (en) | 2012-04-19 | 2017-03-21 | Roche Diabetes Care, Inc. | Devices and methods of determining disturbance variable-corrected analyte concentrations |
EP3412763A4 (en) * | 2016-02-04 | 2019-10-23 | Terumo Kabushiki Kaisha | Blood-sugar-level measurement reagent, blood-sugar-level measurement chip, and blood-sugar-level measurement device set |
US10648015B2 (en) | 2016-02-04 | 2020-05-12 | Terumo Kabushiki Kaisha | Blood glucose measurement reagent, sensor chip, and blood glucose meter set |
US11130982B2 (en) | 2016-02-04 | 2021-09-28 | Terumo Kabushiki Kaisha | Blood glucose measurement reagent, sensor chip, and blood glucose meter set |
EP3578634A4 (en) * | 2017-07-14 | 2020-12-23 | Terumo Kabushiki Kaisha | Blood sugar level measurement chip and blood sugar level measurement device set |
US11719701B2 (en) | 2017-07-14 | 2023-08-08 | Terumo Kabushiki Kaisha | Blood glucose level measuring chip and blood glucose level measuring device set |
JPWO2019171731A1 (en) * | 2018-03-07 | 2021-03-18 | テルモ株式会社 | Methods, reagents and chips for measuring the concentration of analytical objects in biological samples |
EP3736571A4 (en) * | 2018-03-07 | 2021-03-24 | Terumo Kabushiki Kaisha | Method, reagent, and chip, for measuring analyte concentration in biological sample |
JP7208219B2 (en) | 2018-03-07 | 2023-01-18 | テルモ株式会社 | Methods, reagents and chips for measuring analyte concentrations in biological samples |
US11815448B2 (en) | 2018-03-07 | 2023-11-14 | Terumo Kabushiki Kaisha | Method, reagent, and chip for measuring analyte concentration in specimen |
Also Published As
Publication number | Publication date |
---|---|
US7867728B2 (en) | 2011-01-11 |
JP2008510490A (en) | 2008-04-10 |
NO20071564L (en) | 2007-03-26 |
CA2577688A1 (en) | 2006-03-02 |
CN101006185A (en) | 2007-07-25 |
ATE473291T1 (en) | 2010-07-15 |
EP1831392A1 (en) | 2007-09-12 |
TW200621996A (en) | 2006-07-01 |
BRPI0514542A (en) | 2008-06-17 |
RU2007110832A (en) | 2008-10-10 |
MX2007002224A (en) | 2007-05-04 |
DE602005022228D1 (en) | 2010-08-19 |
EP1831392B1 (en) | 2010-07-07 |
US20070275430A1 (en) | 2007-11-29 |
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