WO2006017083A3 - Methods and compositions for phenotype identification based on nucleic acid methylation - Google Patents

Methods and compositions for phenotype identification based on nucleic acid methylation Download PDF

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Publication number
WO2006017083A3
WO2006017083A3 PCT/US2005/023846 US2005023846W WO2006017083A3 WO 2006017083 A3 WO2006017083 A3 WO 2006017083A3 US 2005023846 W US2005023846 W US 2005023846W WO 2006017083 A3 WO2006017083 A3 WO 2006017083A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
tissue
target gene
methylation
gene region
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Application number
PCT/US2005/023846
Other languages
French (fr)
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WO2006017083A2 (en
Inventor
Den Boom Dirk Johannes Van
Mathias Ehrich
Original Assignee
Den Boom Dirk Johannes Van
Mathias Ehrich
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Den Boom Dirk Johannes Van, Mathias Ehrich filed Critical Den Boom Dirk Johannes Van
Priority to EP05768312A priority Critical patent/EP1774027A4/en
Publication of WO2006017083A2 publication Critical patent/WO2006017083A2/en
Publication of WO2006017083A3 publication Critical patent/WO2006017083A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

Abstract

Methods and compositions for identifying an unknown phenotype of a tissue that correlates with changes in the methylation state of the tissue comprising, nucleic acid sample from the tissue with a reagent that modifies unmethylated cytosine to produce uracil, amplifying the nucleic acid target gene region using at least one primer that hybridizes to a strand of said nucleic acid target gene region to produce amplified nucleic acids, determining the characteristic methylation state of the nucleic acid target gene region by base specific cleavage and identification of methylation sites and comparing the ratio of methylated cytosine to unmethylated cytosine for each methylation site of the nucleic acid target gene region to the ratio of methylated cytosine to unmethylated cytosine for each methylation site of a tissue nucleic acid sample of the same type having a known phenotype thereby identifying the unknown phenotype.
PCT/US2005/023846 2004-07-09 2005-07-06 Methods and compositions for phenotype identification based on nucleic acid methylation WO2006017083A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP05768312A EP1774027A4 (en) 2004-07-09 2005-07-06 Methods and compositions for phenotype identification based on nucleic acid methylation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/888,359 US7608394B2 (en) 2004-03-26 2004-07-09 Methods and compositions for phenotype identification based on nucleic acid methylation
US10/888,359 2004-07-09

Publications (2)

Publication Number Publication Date
WO2006017083A2 WO2006017083A2 (en) 2006-02-16
WO2006017083A3 true WO2006017083A3 (en) 2007-11-29

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Family Applications (1)

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PCT/US2005/023846 WO2006017083A2 (en) 2004-07-09 2005-07-06 Methods and compositions for phenotype identification based on nucleic acid methylation

Country Status (3)

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US (1) US7608394B2 (en)
EP (1) EP1774027A4 (en)
WO (1) WO2006017083A2 (en)

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Also Published As

Publication number Publication date
US7608394B2 (en) 2009-10-27
EP1774027A4 (en) 2009-05-27
US20060210992A1 (en) 2006-09-21
EP1774027A2 (en) 2007-04-18
WO2006017083A2 (en) 2006-02-16

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