WO2006014494A3 - Multiple-label fluorescence imaging using excitation-emission matrices - Google Patents

Multiple-label fluorescence imaging using excitation-emission matrices Download PDF

Info

Publication number
WO2006014494A3
WO2006014494A3 PCT/US2005/023994 US2005023994W WO2006014494A3 WO 2006014494 A3 WO2006014494 A3 WO 2006014494A3 US 2005023994 W US2005023994 W US 2005023994W WO 2006014494 A3 WO2006014494 A3 WO 2006014494A3
Authority
WO
WIPO (PCT)
Prior art keywords
excitation
emission
allows
methods
devices
Prior art date
Application number
PCT/US2005/023994
Other languages
French (fr)
Other versions
WO2006014494A2 (en
Inventor
Timothy C Corcoran
Original Assignee
Timothy C Corcoran
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Timothy C Corcoran filed Critical Timothy C Corcoran
Publication of WO2006014494A2 publication Critical patent/WO2006014494A2/en
Publication of WO2006014494A3 publication Critical patent/WO2006014494A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/021—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using plane or convex mirrors, parallel phase plates, or particular reflectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/12—Generating the spectrum; Monochromators
    • G01J3/14—Generating the spectrum; Monochromators using refracting elements, e.g. prisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6423—Spectral mapping, video display
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/10—Scanning
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/10—Scanning
    • G01N2201/104—Mechano-optical scan, i.e. object and beam moving
    • G01N2201/1042—X, Y scan, i.e. object moving in X, beam in Y

Abstract

Methods and devices are disclosed which apply an excitation-emission matrix (EEM) to a heterogeneous, two-dimensional sample, allowing a considerably larger number of emitting, e.g. fluorescent, labels to be used simultaneously. This may be accomplished by employing a spectroscopic method of excitation-emission matrices which allows discrimination of species with similar emission spectra, and also allows positive identification of energy transfer between emitting species. The methods and devices may employ a novel excitation-light scanning technique which allows imaging of the emission from the heterogeneous sample both in two spatial dimensions (length and width) and in two spectral dimensions (excitation and emission wavelength). This light scanning technique maximizes the throughput of excitation light, increasing the sensitivity and hence the reading speed of the instrument.
PCT/US2005/023994 2004-07-07 2005-07-06 Multiple-label fluorescence imaging using excitation-emission matrices WO2006014494A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US58643504P 2004-07-07 2004-07-07
US60/586,435 2004-07-07

Publications (2)

Publication Number Publication Date
WO2006014494A2 WO2006014494A2 (en) 2006-02-09
WO2006014494A3 true WO2006014494A3 (en) 2008-04-10

Family

ID=35787623

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/023994 WO2006014494A2 (en) 2004-07-07 2005-07-06 Multiple-label fluorescence imaging using excitation-emission matrices

Country Status (2)

Country Link
US (1) US7532326B2 (en)
WO (1) WO2006014494A2 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10339311B4 (en) * 2003-08-27 2006-04-27 Leica Microsystems Cms Gmbh System and method for setting a fluorescence spectral measurement system for microscopy
US7715001B2 (en) * 2006-02-13 2010-05-11 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7995202B2 (en) * 2006-02-13 2011-08-09 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US20090262332A1 (en) * 2008-04-18 2009-10-22 Microvast, Inc. High-throughput spectral imaging and spectroscopy apparatus and methods
US20100291706A1 (en) * 2009-05-15 2010-11-18 Millipore Corporation Dye conjugates and methods of use
US20120088262A1 (en) * 2010-10-06 2012-04-12 Millipore Corporation Cyanine compounds, conjugates and method of use
US20170234728A1 (en) * 2010-12-01 2017-08-17 Mks Technology (D/B/A Snowy Range Instruments) Spectrometer
US10448876B2 (en) * 2011-07-26 2019-10-22 Kci Licensing, Inc. Hyperspectral imaging systems and related methods
US8994945B2 (en) 2011-10-27 2015-03-31 Fluid Imaging Technologies, Inc. Method of treatment analysis with particle imaging
US9182351B2 (en) * 2013-11-26 2015-11-10 Nanometrics Incorporated Optical metrology system for spectral imaging of a sample
US9846122B2 (en) 2013-11-26 2017-12-19 Nanometrics Incorporated Optical metrology system for spectral imaging of a sample
US20160338729A1 (en) * 2015-05-19 2016-11-24 Jim Hassett Catheter system for left heart access
WO2017033224A1 (en) * 2015-08-21 2017-03-02 株式会社日立製作所 Light detection device
US9983115B2 (en) 2015-09-21 2018-05-29 Fluid Imaging Technologies, Inc. System and method for monitoring particles in a fluid using ratiometric cytometry
DE102016116377A1 (en) * 2016-09-01 2018-03-01 Rheinisch-Westfälisch Technische Hochschule (RWTH) Aachen Method and device for detecting process parameters in liquid cultures
JP2018128326A (en) * 2017-02-07 2018-08-16 大塚電子株式会社 Optical spectrum measuring device and method of measuring optical spectrum
US10690923B1 (en) * 2017-06-15 2020-06-23 Facebook Technologies, Llc Scanning system for a tiling display
GB2597202B (en) * 2019-05-22 2023-12-13 Hitachi High Tech Corp Analysis system and analysis method
EP3896431A1 (en) * 2020-04-19 2021-10-20 Bernhard W. Adams Device and method for time-resolving hyperspectral imaging spectroscopy

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5776782A (en) * 1994-02-16 1998-07-07 Hamamatsu Photonics K.K. Method for measuring fluorescence resonance energy transfer
US20010046045A1 (en) * 2000-02-18 2001-11-29 Dong Tian Wei Generation of spatially-averaged excitation-emission map in heterogeneous tissue
US20050123979A1 (en) * 2000-08-24 2005-06-09 Shimon Weiss Ultrahigh resolution multi-color colocalization of single fluorescent probes
US20050280817A1 (en) * 2004-04-02 2005-12-22 Uwe Horchner Polychromic laser scanning system and method of use

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5379065A (en) * 1992-06-22 1995-01-03 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Programmable hyperspectral image mapper with on-array processing
US5631734A (en) * 1994-02-10 1997-05-20 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
US5459325A (en) * 1994-07-19 1995-10-17 Molecular Dynamics, Inc. High-speed fluorescence scanner
US5871628A (en) * 1996-08-22 1999-02-16 The University Of Texas System Automatic sequencer/genotyper having extended spectral response
DE19707225A1 (en) * 1997-02-24 1998-08-27 Bodenseewerk Perkin Elmer Co Light scanner
DE19707226A1 (en) * 1997-02-24 1998-08-27 Bodenseewerk Perkin Elmer Co Light scanner
US6160618A (en) * 1998-06-19 2000-12-12 Board Of Regents, The University Of Texas System Hyperspectral slide reader
US6495818B1 (en) * 1998-07-21 2002-12-17 The Institute For Technology Development Microscopic hyperspectral imaging scanner
US6245507B1 (en) * 1998-08-18 2001-06-12 Orchid Biosciences, Inc. In-line complete hyperspectral fluorescent imaging of nucleic acid molecules
US6337472B1 (en) * 1998-10-19 2002-01-08 The University Of Texas System Board Of Regents Light imaging microscope having spatially resolved images
US6373568B1 (en) * 1999-08-06 2002-04-16 Cambridge Research & Instrumentation, Inc. Spectral imaging system
CA2392228A1 (en) * 1999-11-19 2001-05-25 Ming Xiao Compact spectrofluorometer
US6323944B1 (en) * 1999-11-19 2001-11-27 Jobin Yvon, Inc. Compact spectrofluorometer
US6704104B2 (en) * 2000-03-16 2004-03-09 Spectrumedix Llc Multi-wavelength array reader for biological assay
WO2002027297A1 (en) * 2000-09-28 2002-04-04 Sandia Corporation Pulsed laser linescanner for a backscatter absorption gas imaging system
US6870613B1 (en) * 2001-03-07 2005-03-22 Carolyn Tisone Simultaneous recording of multispectral fluorescence signatures
US20020158211A1 (en) * 2001-04-16 2002-10-31 Dakota Technologies, Inc. Multi-dimensional fluorescence apparatus and method for rapid and highly sensitive quantitative analysis of mixtures
US6490075B1 (en) * 2001-08-16 2002-12-03 The United States Of America As Represented By The Secretary Of The Navy Acousto-optic tunable filter hyperspectral imaging system
US6813018B2 (en) * 2002-11-07 2004-11-02 The Boeing Company Hyperspectral imager
EP1660870A2 (en) * 2003-08-26 2006-05-31 Blueshift Biotechnologies, Inc. Time dependent fluorescence measurements
JP4434882B2 (en) * 2004-08-27 2010-03-17 オリンパス株式会社 Laser scanning fluorescence observation system

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5776782A (en) * 1994-02-16 1998-07-07 Hamamatsu Photonics K.K. Method for measuring fluorescence resonance energy transfer
US20010046045A1 (en) * 2000-02-18 2001-11-29 Dong Tian Wei Generation of spatially-averaged excitation-emission map in heterogeneous tissue
US20050123979A1 (en) * 2000-08-24 2005-06-09 Shimon Weiss Ultrahigh resolution multi-color colocalization of single fluorescent probes
US20050280817A1 (en) * 2004-04-02 2005-12-22 Uwe Horchner Polychromic laser scanning system and method of use

Also Published As

Publication number Publication date
US20060007439A1 (en) 2006-01-12
US7532326B2 (en) 2009-05-12
WO2006014494A2 (en) 2006-02-09

Similar Documents

Publication Publication Date Title
WO2006014494A3 (en) Multiple-label fluorescence imaging using excitation-emission matrices
WO2005096749A3 (en) Polychromic laser scanning system and method of use
Niehörster et al. Multi-target spectrally resolved fluorescence lifetime imaging microscopy
US7872752B2 (en) Detector system for unidentified substances
EP1886145A2 (en) Fluorescent detection system and dye set for use therewith
CN101305304A (en) Sensitive emission light gathering and detection system
EP1275954A3 (en) Fluorescence reading apparatus
EP2315005A3 (en) Apparatus and method for fluorescent detection in biological samples
CA2523885A1 (en) Water-based fluorescent ink, recorded image using the same, and judging method
US20150055132A1 (en) Method for calibrating spectroscopy apparatus and equipment for use in the method
Swasey et al. High throughput near infrared screening discovers DNA-templated silver clusters with peak fluorescence beyond 950 nm
WO2008051478A3 (en) Photobleaching by high power pulses
US10712275B2 (en) Hand-held synchronous scan spectrometer for in situ detection of pathogens and mineral deficiency in blood
CN105960466B (en) Non-motorized optical multiplexing for simultaneous detection of DNA-targeted amplicons in polymerase chain reaction solutions
US20100068714A1 (en) Multivariate detection of molecules in biossay
CN115087747A (en) Compositions and methods for spatial analysis of biological materials using time-resolved luminescence measurements
CN109564189A (en) Electrophoretic
Li et al. Autofluorescence detection in analytical chemistry and biochemistry
EP1964610A3 (en) Nucleic acid amplifier
WO2007047555A3 (en) Handheld microarray reader
US10281402B2 (en) Devices and methods for imaging biomolecules
Song et al. The use of a handheld Raman system for virus detection
Huo et al. Dual wavelength excitation fluorescence detector for capillary electrophoresis using a pulsed bi-colour light emitting diode
Kanani et al. Quantitative and Multiplexed Chopper-Based Time-Gated Imaging for Bioanalysis on a Smartphone
Panigrahi et al. Reflective walled sample compartment and optimization of data acquisition geometry towards sensitive fluorescence measurement in fiber-optic setup

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KM KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NG NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SM SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU LV MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
NENP Non-entry into the national phase

Ref country code: DE

WWW Wipo information: withdrawn in national office

Country of ref document: DE

122 Ep: pct application non-entry in european phase