WO2005118814A3 - Metabolically engineered cells for the production of polyunsaturated fatty acids - Google Patents

Metabolically engineered cells for the production of polyunsaturated fatty acids Download PDF

Info

Publication number
WO2005118814A3
WO2005118814A3 PCT/DK2005/000372 DK2005000372W WO2005118814A3 WO 2005118814 A3 WO2005118814 A3 WO 2005118814A3 DK 2005000372 W DK2005000372 W DK 2005000372W WO 2005118814 A3 WO2005118814 A3 WO 2005118814A3
Authority
WO
WIPO (PCT)
Prior art keywords
expression
pufas
fatty acids
heterologous
production
Prior art date
Application number
PCT/DK2005/000372
Other languages
French (fr)
Other versions
WO2005118814A2 (en
WO2005118814A9 (en
Inventor
Jochen Forster
Nina Katrina Gunnarsson
Jens Bredal Nielsen
Original Assignee
Fluxome Sciences As
Jochen Forster
Nina Katrina Gunnarsson
Jens Bredal Nielsen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fluxome Sciences As, Jochen Forster, Nina Katrina Gunnarsson, Jens Bredal Nielsen filed Critical Fluxome Sciences As
Priority to NZ552254A priority Critical patent/NZ552254A/en
Priority to PL05746398T priority patent/PL1766023T3/en
Priority to AU2005250074A priority patent/AU2005250074B2/en
Priority to CA002568689A priority patent/CA2568689A1/en
Priority to EP05746398A priority patent/EP1766023B1/en
Priority to DE602005023308T priority patent/DE602005023308D1/en
Priority to JP2007513686A priority patent/JP4955540B2/en
Priority to AT05746398T priority patent/ATE479757T1/en
Priority to DK05746398.6T priority patent/DK1766023T3/en
Priority to BRPI0511236-2A priority patent/BRPI0511236A/en
Publication of WO2005118814A2 publication Critical patent/WO2005118814A2/en
Publication of WO2005118814A9 publication Critical patent/WO2005118814A9/en
Publication of WO2005118814A3 publication Critical patent/WO2005118814A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
    • A23L33/12—Fatty acids or derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
    • A61K8/361—Carboxylic acids having more than seven carbon atoms in an unbroken chain; Salts or anhydrides thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67—Vitamins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6431—Linoleic acids [18:2[n-6]]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6481—Phosphoglycerides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Medicinal Chemistry (AREA)
  • Birds (AREA)
  • Emergency Medicine (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Dermatology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Biophysics (AREA)
  • Polymers & Plastics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cosmetics (AREA)
  • Fats And Perfumes (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The present invention relates to the construction and engineering of cells, more particularly microorganisms for producing PUFAs with four or more double bonds from non-fatty acid substrates through heterologous expression of an oxygen requiring pathway. The invention especially involves improvement of the PUFA content in the host organism through fermentation optimization, e.g. decreasing the temperature and/or designing an optimal medium, or through improving the flux towards fatty acids by metabolic engineering, e.g. through over-expression of fatty acid synthases, over-expression of other enzymes involved in biosynthesis of the precursors for PUFAs, or codon optimization of the heterologous genes, or expression of heterologous enzymes involved in the biosynthesis of the precursor for PUFAs.
PCT/DK2005/000372 2004-06-04 2005-06-03 Metabolically engineered cells for the production of polyunsaturated fatty acids WO2005118814A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
NZ552254A NZ552254A (en) 2004-06-04 2005-06-03 Metabolically engineered cells for the production of polyunsaturated fatty acids
PL05746398T PL1766023T3 (en) 2004-06-04 2005-06-03 Metabolically engineered cells for the production of polyunsaturated fatty acids
AU2005250074A AU2005250074B2 (en) 2004-06-04 2005-06-03 Metabolically engineered cells for the production of polyunsaturated fatty acids
CA002568689A CA2568689A1 (en) 2004-06-04 2005-06-03 Metabolically engineered cells for the production of polyunsaturated fatty acids
EP05746398A EP1766023B1 (en) 2004-06-04 2005-06-03 Metabolically engineered cells for the production of polyunsaturated fatty acids
DE602005023308T DE602005023308D1 (en) 2004-06-04 2005-06-03 METABOLICALLY MANUFACTURED CELLS FOR THE PRODUCTION OF MULTIPLE UNSATURATED FATTY ACIDS
JP2007513686A JP4955540B2 (en) 2004-06-04 2005-06-03 Metabolically modified cells for polyunsaturated fatty acid production
AT05746398T ATE479757T1 (en) 2004-06-04 2005-06-03 CELLS MANUFACTURED BY METABOLIC TECHNOLOGY FOR THE PRODUCTION OF POLYUNSATURATED FATTY ACIDS
DK05746398.6T DK1766023T3 (en) 2004-06-04 2005-06-03 Metabolically engineered cells for the production of polyunsaturated fatty acids
BRPI0511236-2A BRPI0511236A (en) 2004-06-04 2005-06-03 A method for producing four or more double bond polyunsaturated fatty acids, genetically modified saccharomyces cerevisiae which is capable of producing four or more double bond polyunsaturated fatty acids when developed on a substrate other than fatty acid, composition, use of the composition and use of a genetically modified saccharomyces cervisiae

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US57724504P 2004-06-04 2004-06-04
US60/577,245 2004-06-04

Publications (3)

Publication Number Publication Date
WO2005118814A2 WO2005118814A2 (en) 2005-12-15
WO2005118814A9 WO2005118814A9 (en) 2006-02-09
WO2005118814A3 true WO2005118814A3 (en) 2006-03-09

Family

ID=34956349

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2005/000372 WO2005118814A2 (en) 2004-06-04 2005-06-03 Metabolically engineered cells for the production of polyunsaturated fatty acids

Country Status (14)

Country Link
US (1) US7736884B2 (en)
EP (1) EP1766023B1 (en)
JP (1) JP4955540B2 (en)
AT (1) ATE479757T1 (en)
AU (1) AU2005250074B2 (en)
BR (1) BRPI0511236A (en)
CA (1) CA2568689A1 (en)
DE (1) DE602005023308D1 (en)
DK (1) DK1766023T3 (en)
ES (1) ES2351973T3 (en)
NZ (1) NZ552254A (en)
PL (1) PL1766023T3 (en)
PT (1) PT1766023E (en)
WO (1) WO2005118814A2 (en)

Families Citing this family (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0408215B1 (en) * 2003-03-10 2015-11-03 Broin And Associates Inc process for ethanol production using raw starch
US20050233030A1 (en) * 2004-03-10 2005-10-20 Broin And Associates, Inc. Methods and systems for producing ethanol using raw starch and fractionation
WO2005021761A1 (en) 2003-08-21 2005-03-10 Monsanto Technology Llc Fatty acid desaturases from primula
EP1734947B1 (en) 2004-04-16 2015-04-15 Monsanto Technology, LLC Expression of fatty acid desaturases in corn
ES2715640T3 (en) 2004-04-22 2019-06-05 Commw Scient Ind Res Org Synthesis of long chain polyunsaturated fatty acids by recombinant cells
CN103451246B (en) 2004-04-22 2018-02-16 联邦科学技术研究组织 With recombinant cell synthesis of long-chain polyunsaturated fatty acids
WO2005113785A2 (en) * 2004-05-13 2005-12-01 Novozymes North America, Inc. A process of producing a fermentation product
JP4587451B2 (en) 2004-08-20 2010-11-24 サントリーホールディングス株式会社 Polypeptide having ω3 fatty acid desaturation activity, polynucleotide encoding the polypeptide, and use thereof
GB0503657D0 (en) 2005-02-22 2005-03-30 Fluxome Sciences As Metabolically engineered cells for the production of resveratrol or an oligomeric or glycosidically-bound derivative thereof
DE102005038036A1 (en) * 2005-08-09 2007-02-15 Basf Plant Science Gmbh Process for the preparation of arachidonic acid and / or eicosapentaenoic acid in transgenic crops
US7919289B2 (en) 2005-10-10 2011-04-05 Poet Research, Inc. Methods and systems for producing ethanol using raw starch and selecting plant material
GB0603160D0 (en) * 2006-02-16 2006-03-29 Rothamsted Res Ltd Nucleic acid
AU2013219144B2 (en) * 2006-02-16 2016-08-04 Basf Plant Science Gmbh Nucleic acid
US7695950B2 (en) * 2006-05-17 2010-04-13 E. I. Du Pont De Nemours And Company Δ5 desaturase and its use in making polyunsaturated fatty acids
WO2008000277A2 (en) * 2006-06-29 2008-01-03 Fluxome Sciences A/S Metabolically engineered fungal cells with increased content of polyunsaturated fatty acids
EP2041275B1 (en) * 2006-07-19 2011-05-04 Monsanto Technology, LLC Fatty acid desaturases from tetraselmis suecica
GB0614442D0 (en) 2006-07-20 2006-08-30 Fluxome Sciences As Metabolically engineered cells for the production of pinosylvin
SG10201600157QA (en) 2006-07-21 2016-02-26 Xyleco Inc Conversion systems for biomass
AU2007287585B2 (en) * 2006-08-24 2013-08-29 Basf Plant Science Gmbh Isolation and characterization of a novel Pythium omega 3 desaturase with specificity to all omega 6 fatty acids longer than 18 carbon chains
WO2008025068A1 (en) 2006-08-29 2008-03-06 Commonwealth Scientific And Industrial Research Organisation Synthesis of fatty acids
WO2008073275A2 (en) 2006-12-07 2008-06-19 E. I. Du Pont De Nemours And Company Mutant delta-8 desaturase genes engineered by targeted mutagensis and their use in making polyunsaturated fatty acids
US7709239B2 (en) * 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
US8957280B2 (en) * 2007-05-03 2015-02-17 E. I. Du Pont De Nemours And Company Delta-5 desaturases and their use in making polyunsaturated fatty acids
JP2010533003A (en) * 2007-07-13 2010-10-21 オーシャン ニュートリッション カナダ リミテッド D4 desaturase and D5 elongase
US8551744B2 (en) 2007-07-23 2013-10-08 Suntory Holdings Limited Method of preparing a fatty acid composition
AU2008314885B2 (en) 2007-10-26 2013-11-07 Suntory Holdings Limited Novel ATP:citrate lyase genes
US8343753B2 (en) 2007-11-01 2013-01-01 Wake Forest University School Of Medicine Compositions, methods, and kits for polyunsaturated fatty acids from microalgae
US20100010088A1 (en) * 2007-11-01 2010-01-14 Wake Forest University School Of Medicine Compositions and Methods for Prevention and Treatment of Mammalian Diseases
GB0725075D0 (en) * 2007-12-21 2008-01-30 Fluxome Sciences As Metabolically engineered fungal cells with increased content of polyunsaturated fatty acids
EP2108702A1 (en) * 2008-04-07 2009-10-14 Organo Balance GmbH Synthesis of polyunsaturated fatty acids in yeast
EP2310517B1 (en) 2008-07-11 2016-03-16 Institut National De La Recherche Agronomique (INRA) New mutant yeast strains capable of accumulating a large quantity of lipids
CA3082380A1 (en) 2008-11-18 2010-05-27 Commonwealth Scientific And Industrial Research Organisation Recombinant cells comprisng exogenous .delta.6 desaturase and methods for producing oil containing unsaturated fatty acids
WO2010067352A1 (en) * 2008-12-09 2010-06-17 Ben Gurion University Of The Negev Research And Development Authority Polyunsaturated fatty acid elongase
EP2896700B1 (en) * 2009-03-03 2017-08-02 POET Research, Inc. Fermentation system for producing ethanol from xylose
US9068206B1 (en) 2009-03-03 2015-06-30 Poet Research, Inc. System for treatment of biomass to facilitate the production of ethanol
US8450094B1 (en) 2009-03-03 2013-05-28 Poet Research, Inc. System for management of yeast to facilitate the production of ethanol
WO2010102060A2 (en) * 2009-03-03 2010-09-10 Poet Research, Inc. System for pre-treatment of biomass for the production of ethanol
AU2010321249B2 (en) * 2009-11-20 2015-04-09 BASF Agricultural Solutions Seed US LLC Brassica plants comprising mutant FAD3 alleles
EP2327776A1 (en) 2009-11-30 2011-06-01 Institut National De La Recherche Agronomique Method for the production of Very Long Chain Fatty Acids (VLCFA) by fermentation with a recombinant Yarrowia sp
WO2011079388A1 (en) 2009-12-30 2011-07-07 Iogen Energy Corporation Modified yeast strains exhibiting enhanced fermentation of lignocellulosic hydrolysates
CA2791695C (en) 2010-03-02 2018-04-24 Massachusetts Institute Of Technology Microbial engineering for the production of fatty acids and fatty acid derivatives
EP2561050A1 (en) 2010-04-22 2013-02-27 E.I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
GB201008826D0 (en) 2010-05-26 2010-07-14 Fluxome Sciences As Production of metabolites
UA115302C2 (en) * 2010-06-24 2017-10-25 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Lowering saturated fatty acid content of plant seeds
FR2962133B1 (en) * 2010-07-01 2014-09-12 Agronomique Inst Nat Rech OPTIMIZATION OF THE SYNTHESIS AND ACCUMULATION OF LIPIDS
EP3382015B8 (en) * 2010-08-06 2021-11-03 Melt&Marble AB Methods and products for production of wax esters
WO2012037215A2 (en) * 2010-09-15 2012-03-22 Wisconsin Alumni Research Foundation Recombinant yeast with improved ethanol tolerance and related methods of use
US20140206058A1 (en) * 2011-03-08 2014-07-24 Poet Research, Inc. Systems and methods for improving stillage
US20120247066A1 (en) 2011-04-01 2012-10-04 Ice House America, Llc Ice bagging apparatus and methods
CN102220351A (en) * 2011-04-04 2011-10-19 山东农业大学 Function identification and application of delta-5 desaturase gene PinD5 of Phytophthora infestans in potato
CA2833400A1 (en) 2011-05-26 2012-11-29 E.I. Dupont De Nemours And Company Expression of caleosin in recombinant oleaginous microorganisms to increase oil content therein
CN109468241A (en) * 2011-10-19 2019-03-15 麻省理工学院 Engineered microorganism and the method for microbial oil production
US8790901B2 (en) 2011-12-14 2014-07-29 Pronutria, Inc. Microorganisms and methods for producing unsaturated fatty acids
CN102660576B (en) * 2012-04-12 2014-06-11 中国人民解放军军事医学科学院生物工程研究所 Method for producing DHA in mammal cells
EP3266316A1 (en) 2012-06-15 2018-01-10 Commonwealth Scientific and Industrial Research Organisation Production of long chain polyunsaturated fatty acids in plant cells
CN102787128A (en) * 2012-08-28 2012-11-21 江南大学 Desaturase delta9 Des for polyunsaturated fatty acid biosynthesis
NZ709083A (en) 2012-12-24 2020-07-31 Qualitas Health Inc Eicosapentaenoic acid (epa) formulations
US9340767B2 (en) 2013-03-13 2016-05-17 Poet Research, Inc. Propagating an organism and related methods and compositions
US9034631B2 (en) 2013-03-14 2015-05-19 Poet Research, Inc. Systems and methods for yeast propagation
BR112015030015B8 (en) * 2013-05-28 2023-01-10 Poet Res Incorporated METHOD AND SYSTEM FOR PROPAGATION OF ETHANOLGEN FOR USE IN THE PRODUCTION OF A FERMENTATION PRODUCT COMPRISING BIOMASS ETHANOL
US10174296B2 (en) 2013-08-27 2019-01-08 Novogy, Inc. Microorganisms engineered to use unconventional sources of phosphorous or sulfur
MX2016007054A (en) * 2013-12-05 2016-09-06 Reg Life Sciences Llc Microbial production of fatty amines.
MX2016008270A (en) 2013-12-18 2017-05-26 Commw Scient Ind Res Org Lipid comprising long chain polyunsaturated fatty acids.
WO2015196250A1 (en) 2014-06-27 2015-12-30 Commonwealth Scientific And Industrial Research Organisation Lipid comprising docosapentaenoic acid
US9752165B2 (en) * 2014-02-10 2017-09-05 Cellulosic Ethanol Technologies, Llc Processes and systems for recovering oil from fermentation products
AU2015344980B2 (en) 2014-11-14 2021-11-11 Basf Plant Science Company Gmbh Materials and methods for increasing the tocopherol content in seed oil
AU2016269143A1 (en) 2015-05-28 2017-11-30 Evolva Sa Biosynthesis of phenylpropanoids and phenylpropanoid derivatives
US10513718B2 (en) 2017-06-06 2019-12-24 City University Of Hong Kong Method of producing polyunsaturated fatty acid
CA3085746A1 (en) 2017-12-14 2019-06-20 Poet Research, Inc. Method for propagating microorganisms on a medium comprising stillage
CN109234179B (en) * 2018-10-29 2021-11-12 贵州茅台酒股份有限公司 Yeast separation medium in fermented grains and preparation method thereof
WO2020094750A1 (en) * 2018-11-09 2020-05-14 Evonik Operations Gmbh Method for producing a biomass with an increased content of polyunsaturated fatty acids
CN109929870B (en) * 2019-02-20 2021-03-16 天津大学 Application of sugar metabolism and lipid metabolism for synergistically improving yield of fatty acid derivatives synthesized by yarrowia lipolytica
CN111218409A (en) * 2019-11-27 2020-06-02 江西科技师范大学 High-salt-tolerance saccharomyces cerevisiae strain, and construction method and application thereof
CN116034164A (en) * 2020-03-31 2023-04-28 倍耐力轮胎股份公司 Method for engineering oleaginous microorganisms
CN112481147B (en) * 2020-12-10 2022-04-29 科稷达隆(北京)生物技术有限公司 Yeast mutant with function deletion of glutamine synthetase gene and preparation method and application thereof
WO2022169884A2 (en) * 2021-02-02 2022-08-11 Allen Institute Artificial expression constructs for modulating gene expression in gabaergic neurons and astrocytes
CN116121092A (en) * 2022-11-01 2023-05-16 四川大学 Recombinant saccharomyces cerevisiae with enhanced multiple stress resistance, construction method and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020138874A1 (en) * 1998-09-02 2002-09-26 Pradip Mukerji Elongase genes and uses thereof
WO2004005442A1 (en) * 2002-07-03 2004-01-15 Basf Plant Science Gmbh Method for the production of conjugated polyunsaturated fatty acids comprising at least two double bonds in plants

Family Cites Families (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR8108820A (en) 1980-09-24 1982-08-24 Cetus Corp DIAGNOSTIC PROCESS AND PROBE
US4582788A (en) * 1982-01-22 1986-04-15 Cetus Corporation HLA typing method and cDNA probes used therein
EP0084796B1 (en) 1982-01-22 1990-05-02 Cetus Corporation Hla typing method and cdna probes used therein
US4683194A (en) * 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CA1338457C (en) 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US5057419A (en) * 1988-09-22 1991-10-15 Rutgers University Genetically engineered plasmid and organisms for the production of specialized oils
US6355861B1 (en) * 1991-10-10 2002-03-12 Rhone-Poulenc Agrochimie Production of gamma linolenic acid by a Δ6-desaturase
JPH0614667A (en) * 1992-03-13 1994-01-25 Lubrizol Corp:The Modification of vegetable oil by using desaturase
CA2149223C (en) 1992-11-17 2007-04-24 Jonathan Edward Lightner Genes for microsomal delta-12 fatty acid desaturases and related enzymes from plants
US6872872B1 (en) * 1992-11-17 2005-03-29 E. I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and related enzymes from plants
US6372965B1 (en) * 1992-11-17 2002-04-16 E.I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and hydroxylases from plants
US5869304A (en) * 1994-10-12 1999-02-09 University Of Kentucky Research Foundation Technique for specifying the fatty acid at the sn2 position of acylglycerol lipids
GB9510927D0 (en) * 1995-05-31 1995-07-26 Ca Nat Research Council Plant and seed oil modification
EP0794250A1 (en) * 1996-03-04 1997-09-10 Soremartec S.A. Isolation and sequencing of the hazel FAd2-N gene
US5850026A (en) * 1996-07-03 1998-12-15 Cargill, Incorporated Canola oil having increased oleic acid and decreased linolenic acid content
US6194167B1 (en) * 1997-02-18 2001-02-27 Washington State University Research Foundation ω-3 fatty acid desaturase
US6432684B1 (en) 1997-04-11 2002-08-13 Abbott Laboratories Human desaturase gene and uses thereof
US5968809A (en) * 1997-04-11 1999-10-19 Abbot Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US5972664A (en) * 1997-04-11 1999-10-26 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
JP4087460B2 (en) 1997-04-11 2008-05-21 カルジーン エル エル シー Methods and compositions for the synthesis of long chain polyunsaturated fatty acids in plants
US6428990B1 (en) * 1997-04-11 2002-08-06 Abbott Laboratories Human desaturase gene and uses thereof
WO1999011806A1 (en) * 1997-09-04 1999-03-11 Rutgers, The State University Of New Jersey Pathogen-resistant transgenic plants and methods of making
GB9724783D0 (en) 1997-11-24 1998-01-21 Inst Arable Crops Research Novel polypeptides
CA2315297A1 (en) 1997-12-23 1999-07-08 University Of Bristol Desaturase
US6492108B1 (en) * 1998-03-26 2002-12-10 Incyte Genomics, Inc. Delta-6 desaturase homologs
AU5785199A (en) * 1998-08-24 2000-03-14 Rutgers, The State University Of New Jersey Synthetic fatty acid desaturase gene for expression in plants
US20030163845A1 (en) 1998-09-02 2003-08-28 Pradip Mukerji Elongase genes and uses thereof
US6403349B1 (en) * 1998-09-02 2002-06-11 Abbott Laboratories Elongase gene and uses thereof
US6913916B1 (en) * 1998-09-02 2005-07-05 Abbott Laboratories Elongase genes and uses thereof
ATE353952T1 (en) 1998-12-07 2007-03-15 Univ Washington DESATURASES AND METHOD OF THEIR USE FOR THE SYNTHESIS OF POLYUNSATURATED FATTY ACIDS
US20020151019A1 (en) * 1999-01-19 2002-10-17 John Shanklin Mutant fatty acid desaturase and methods for directed mutagenesis
US6686186B2 (en) * 1999-01-19 2004-02-03 Brookhaven Science Associates Llc Mutant fatty acid desaturase
WO2000055330A1 (en) 1999-03-18 2000-09-21 The University Of Bristol Polysaturated fatty acid (pufa) elongase from caenorhabditis elegans
KR20020048910A (en) * 1999-05-21 2002-06-24 카길 다우 엘엘씨 Method and materials for the synthesis of organic products
CA2378423A1 (en) 1999-07-06 2001-01-11 Basf Plant Science Gmbh Plants expressing .delta.6-desaturase genes and oils from these plants containing pufas and method for producing unsaturated fatty acids
WO2001004636A1 (en) 1999-07-12 2001-01-18 Ohio University Mammalian cells expressing desaturases and elongases
US7070970B2 (en) * 1999-08-23 2006-07-04 Abbott Laboratories Elongase genes and uses thereof
BR0013607A (en) 1999-08-26 2002-04-30 Calgene Llc Nucleic acid sequences and processes of use for the production of plants with modified polyunsaturated fatty acids
FR2798391B1 (en) 1999-09-15 2001-12-14 Inst Nat Sante Rech Med THYROID ENZYME NADPH OXYDASE, NUCLEIC ACID ENCODING THIS ENZYME AND THEIR APPLICATIONS
DE19950589A1 (en) * 1999-10-20 2001-05-23 Gvs Ges Fuer Erwerb Und Verwer Elongase promoters for tissue-specific expression of transgenes in plants
GB9929897D0 (en) 1999-12-18 2000-02-09 Slabas Antoni R Improvements in or relating to conjugated fatty acids and related compounds
US20030172398A1 (en) * 1999-12-21 2003-09-11 Browse John A. Novel delta-12 desaturase and methods of using it for synthesis of polyunsaturated fatty acids
CZ20022502A3 (en) 2000-02-09 2002-10-16 Basf Aktiengesellschaft Elongase gene and process for preparing multiple-unsaturated fatty acids
WO2001075069A1 (en) 2000-03-31 2001-10-11 Idemitsu Petrochemical Co., Ltd. Process for producing lipids and lipid-secreting microorganisms
WO2001079499A1 (en) 2000-04-18 2001-10-25 Commonwealth Scientific And Industrial Research Organisation Method of modifying the content of cottonseed oil
AU2001264161A1 (en) * 2000-05-09 2001-11-20 Bioriginal Food And Science Corp. Production of conjugated linoleic and linolenic acids in plants
ATE510908T1 (en) 2000-09-28 2011-06-15 Bioriginal Food & Science Corp FAD5-2, MEMBER OF THE DESATURASE FAMILY AND USES THEREOF
JP2004512049A (en) 2000-10-26 2004-04-22 キセノン　ジェネティックス　インコーポレイテッド Method for screening compounds that regulate lipid metabolism
EP1339852A2 (en) * 2000-11-22 2003-09-03 Cargill Dow LLC Methods and materials for the synthesis of organic products
CA2429469A1 (en) 2000-11-29 2002-06-06 Xenon Genetics Inc. Human elongase genes and uses thereof
DE10102338A1 (en) * 2001-01-19 2002-07-25 Basf Plant Science Gmbh New expression cassette for plant genes, useful for preparing transgenic plants that have increased production of polyunsaturated fatty acids
DE10102337A1 (en) * 2001-01-19 2002-07-25 Basf Plant Science Gmbh Preparing ester containing polyunsaturated fatty acids, useful e.g. in animal or human nutrition, by transforming organism with desaturase gene from Phaeodactylum tricornatum
US6635451B2 (en) * 2001-01-25 2003-10-21 Abbott Laboratories Desaturase genes and uses thereof
EP1392823B1 (en) 2001-01-25 2011-11-30 Abbott Laboratories Desaturase genes and uses thereof
AU2002255657A1 (en) * 2001-03-02 2002-09-19 Ross Carlson Production of polyhydroxyalkanoates
US7238851B2 (en) 2001-03-12 2007-07-03 The General Hospital Corporation Non-human transgenic mammals expressing an n-3 desaturase gene
GB0107510D0 (en) 2001-03-26 2001-05-16 Univ Bristol New elongase gene and a process for the production of -9-polyunsaturated fatty acids
WO2002081702A1 (en) 2001-04-05 2002-10-17 Idemitsu Technofine Co., Ltd. Fatty acid δ6-position desaturase genes and plasmids and tra nsformant containing these genes
US7045683B2 (en) * 2001-05-04 2006-05-16 Abbott Laboratories Δ4-desaturase genes and uses thereof
US7193134B2 (en) * 2001-06-06 2007-03-20 Bioriginal Food & Science Corp. Flax (Linum usitatissimum L.) fatty acid desaturase
DE10137374A1 (en) 2001-07-31 2003-02-27 Ipk Inst Fuer Pflanzengenetik Process for the production of arachidonic acid in transgenic organisms
US6875595B2 (en) * 2001-09-13 2005-04-05 Divergence, Inc. Nematode fatty acid desaturase-like sequences
US7211656B2 (en) * 2002-01-30 2007-05-01 Abbott Laboratories Desaturase genes, enzymes encoded thereby, and uses thereof
WO2003064638A2 (en) 2002-01-30 2003-08-07 Basf Plant Science Gmbh Elongase gene and method for producing polyunsaturated fatty acids
GB2385852A (en) 2002-02-27 2003-09-03 Rothamsted Ex Station Delta 6-desaturases from Primulaceae
US7700833B2 (en) * 2002-03-01 2010-04-20 Cornell University Process for the production of unsaturated fatty acids
ES2204328B1 (en) * 2002-10-04 2005-09-01 Consejo Sup. De Invest. Cientificas PROCEDURE FOR THE PRODUCTION OF POLINSATURATED FATTY ACIDS WITH LEAVES BY INCORPORATION OF OLEFINIC OR ACETYLENE SUBSTRATES.
ES2525212T3 (en) * 2002-12-19 2014-12-19 University Of Bristol Novel procedure for the production of polyunsaturated fatty acids
AU2004215705B2 (en) * 2003-02-27 2009-11-12 Basf Plant Science Gmbh Method for the production of polyunsaturated fatty acids
US7238482B2 (en) * 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7125672B2 (en) * 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7214491B2 (en) * 2003-05-07 2007-05-08 E. I. Du Pont De Nemours And Company Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts
US7259255B2 (en) * 2003-06-25 2007-08-21 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7267976B2 (en) * 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
US7504259B2 (en) * 2003-11-12 2009-03-17 E. I. Du Pont De Nemours And Company Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast
US7465564B2 (en) * 2004-08-10 2008-12-16 E.I. Du Pont De Nemours And Company Production of γ-linolenic acid in oleaginous yeast
US7198937B2 (en) * 2004-11-04 2007-04-03 E. I. Du Pont De Nemours And Company Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7550286B2 (en) * 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
US7273746B2 (en) * 2004-11-04 2007-09-25 E.I. Dupont De Nemours And Company Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020138874A1 (en) * 1998-09-02 2002-09-26 Pradip Mukerji Elongase genes and uses thereof
WO2004005442A1 (en) * 2002-07-03 2004-01-15 Basf Plant Science Gmbh Method for the production of conjugated polyunsaturated fatty acids comprising at least two double bonds in plants

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ABBADI A ET AL: "Transgenic oilseeds as sustainable source of nutritionally relevant C20 and C22 polyunsaturated fatty acids?", EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, WILEY VCH VERLAG, WEINHEIM, DE, vol. 103, no. 2, February 2001 (2001-02-01), pages 106 - 113, XP002228744, ISSN: 1438-7697 *
BEAUDOIN FREDERIC ET AL: "Heterologous reconstitution in yeast of the polyunsaturated fatty acid biosynthetic pathway", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 97, no. 12, 6 June 2000 (2000-06-06), pages 6421 - 6426, XP002200201, ISSN: 0027-8424 *
DOMERGUE FREDERIC ET AL: "Acyl carriers used as substrates by the desaturases and elongases involved in very long-chain polyunsaturated fatty acids biosynthesis reconstituted in yeast.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 37, 12 September 2003 (2003-09-12), pages 35115 - 35126, XP002313880, ISSN: 0021-9258 *
QI BAOXIU ET AL: "Production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in plants", NATURE BIOTECHNOLOGY, vol. 22, no. 6, June 2004 (2004-06-01), pages 739 - 745, XP002352107, ISSN: 1087-0156 *
WONGWATHANARAT PRASERT ET AL: "Two fatty acid DELTA9-desaturase genes, ole1 and ole2, from Mortierella alpina complement the yeast ole1 mutation", MICROBIOLOGY (READING), vol. 145, no. 10, October 1999 (1999-10-01), pages 2939 - 2946, XP002352108, ISSN: 1350-0872 *

Also Published As

Publication number Publication date
US7736884B2 (en) 2010-06-15
AU2005250074B2 (en) 2011-04-14
PL1766023T3 (en) 2011-04-29
DE602005023308D1 (en) 2010-10-14
WO2005118814A2 (en) 2005-12-15
AU2005250074A1 (en) 2005-12-15
EP1766023B1 (en) 2010-09-01
ATE479757T1 (en) 2010-09-15
BRPI0511236A (en) 2007-11-27
JP2008501323A (en) 2008-01-24
JP4955540B2 (en) 2012-06-20
PT1766023E (en) 2010-12-09
ES2351973T3 (en) 2011-02-14
EP1766023A2 (en) 2007-03-28
US20060051847A1 (en) 2006-03-09
NZ552254A (en) 2008-12-24
DK1766023T3 (en) 2010-12-13
CA2568689A1 (en) 2005-12-15
WO2005118814A9 (en) 2006-02-09

Similar Documents

Publication Publication Date Title
WO2005118814A3 (en) Metabolically engineered cells for the production of polyunsaturated fatty acids
Kikukawa et al. Arachidonic acid production by the oleaginous fungus Mortierella alpina 1S-4: A review
Gemperlein et al. Polyunsaturated fatty acid production by Yarrowia lipolytica employing designed myxobacterial PUFA synthases
Spagnuolo et al. Oleaginous yeast for biofuel and oleochemical production
Daskalaki et al. Laboratory evolution strategies for improving lipid accumulation in Yarrowia lipolytica
Cui et al. Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp. SD116
Liu et al. Sesamol enhances cell growth and the biosynthesis and accumulation of docosahexaenoic acid in the microalga Crypthecodinium cohnii
Sakuradani et al. Improved production of various polyunsaturated fatty acids through filamentous fungus Mortierella alpina breeding
Shi et al. Application of a delta-6 desaturase with α-linolenic acid preference on eicosapentaenoic acid production in Mortierella alpina
Liu et al. Improved production of arachidonic acid by combined pathway engineering and synthetic enzyme fusion in Yarrowia lipolytica
Sakuradani et al. Single cell oil production by Mortierella alpina
NZ593097A (en) Enzymes and methods for producing omega-3 fatty acids
BRPI0410510A (en) isolated nucleic acid molecules, chimeric gene, yarrowia sp. Y-linolenic, stearidonic, eicosatetraenoic, eicosapentaenoic, dihomo-y-linoleic, docosapentaenoic acid production methods, gene optimization method, microbial oil and gene expression optimization methods
WO2005103253A8 (en) Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
WO2005047480A3 (en) Delta-15 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous plants and yeast
WO2009031766A3 (en) Mutants having capability to produce 1,4-butanediol and method for preparing 1,4-butanediol using the same
Khan et al. Construction of DGLA producing cell factory by genetic modification of Mucor circinelloides
WO2004104167A3 (en) A δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts
WO2013168310A1 (en) Method for producing oxo fatty acid and rare fatty acid
Fazili et al. Mucor circinelloides: a model organism for oleaginous fungi and its potential applications in bioactive lipid production
IL175521A0 (en) Process for the cultivation of microorganisms of the genus thraustochytriales
Shi et al. Identification of a novel C22-∆ 4-producing docosahexaenoic acid (DHA) specific polyunsaturated fatty acid desaturase gene from Isochrysis galbana and its expression in Saccharomyces cerevisiae
AU2011266953A8 (en) Production of Biodiesel by yeast from lignocellulose and glycerol
Tang et al. Characterization of an omega-3 desaturase from Phytophthora parasitica and application for eicosapentaenoic acid production in Mortierella alpina
WO2009157702A3 (en) Method for preparing polylactate and copolymer thereof using a mutant microorganism with enhanced polylactate, and the copolymer producing capability thereof

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KM KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NG NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SM SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
COP Corrected version of pamphlet

Free format text: PAGES 1/91-91/91, SEQUENCE LISTING, REPLACED BY NEW PAGES 1/89-89/89; AFTER RECTIFICATION OF OBVIOUS ERRORS AUTHORIZED BY THE INTERNATIONAL SEARCH AUTHORITY

DPEN Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed from 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2005250074

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2568689

Country of ref document: CA

Ref document number: 2007513686

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: DE

WWW Wipo information: withdrawn in national office

Ref document number: DE

ENP Entry into the national phase

Ref document number: 2005250074

Country of ref document: AU

Date of ref document: 20050603

Kind code of ref document: A

WWP Wipo information: published in national office

Ref document number: 2005250074

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2005746398

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 552254

Country of ref document: NZ

WWE Wipo information: entry into national phase

Ref document number: 23/DELNP/2007

Country of ref document: IN

WWP Wipo information: published in national office

Ref document number: 2005746398

Country of ref document: EP

ENP Entry into the national phase

Ref document number: PI0511236

Country of ref document: BR